Restriction map for
pFN21AE0378
(Circular) (Six-base) MAPSORT of: pFN21AE0378.seq Check: 2433 from: 1 to: 6448
pFN21AE0378 6448 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4258 278
Size: 53 83 186 936 2722 2468
Fragments arranged by size:
2722 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3125 3791 1937
Size: 1188 666 4594
Fragments arranged by size:
4594 1188 666
Acc65I G'GTAC_C
Cuts at: 3769 3769
Size: 6448
AclI AA'CG_TT
Cuts at: 1563 3196 4575 4948 1563
Size: 1633 1379 373 3063
Fragments arranged by size:
3063 1633 1379 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2511 4511 5895 6163 1632
Size: 879 2000 1384 268 1917
Fragments arranged by size:
2000 1917 1384 879 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6251
AflIII A'CryG_T
Cuts at: 1203 3140 3609 5347 6324 1203
Size: 1937 469 1738 977 1327
Fragments arranged by size:
1937 1738 1327 977 469
AgeI A'CCGG_T
Cuts at: 5279 5279
Size: 6448
AhdI GACnn_n'nnGTC
Cuts at: 5177 5177
Size: 6448
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6448
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2187 2190 1184
Size: 1003 3 5442
Fragments arranged by size:
5442 1003 3
AlwNI CAG_nnn'CTG
Cuts at: 3341 4191 5763 3341
Size: 850 1572 4026
Fragments arranged by size:
4026 1572 850
ApaLI G'TGCA_C
Cuts at: 4505 5661 4505
Size: 1156 5292
ApoI r'AATT_y
Cuts at: 1493 2021 2102 2257 2342 2352 2732 3259 3566
Size: 528 81 155 85 10 380 527 307
Cuts at: 3566 3757 3983 4135 6244 1493
Size: 191 226 152 2109 1697
Fragments arranged by size:
2109 1697 528 527 380 307 226 191
155 152 85 81 10
AseI AT'TA_AT
Cuts at: 160 1032 5002 160
Size: 872 3970 1606
Fragments arranged by size:
3970 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6448
AvaI C'yCGr_G
Cuts at: 1870 1943 3773 1870
Size: 73 1830 4545
Fragments arranged by size:
4545 1830 73
BamHI G'GATC_C
Cuts at: 3778 4145 3778
Size: 367 6081
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3439 3769 5224 618
Size: 325 217 627 1652 330 1455 1842
Fragments arranged by size:
1842 1652 1455 627 330 325 217
BanII G_rGCy'C
Cuts at: 1391 3767 1391
Size: 2376 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5477
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4639 4673 1903
Size: 34 2702 34 3678
Fragments arranged by size:
3678 2702 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4340 5550 4340
Size: 1210 5238
BclI T'GATC_A
Cuts at: 1548 2191 3598 1548
Size: 643 1407 4398
Fragments arranged by size:
4398 1407 643
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5059 136
Size: 107 122 71 631 605 3387 1525
Fragments arranged by size:
3387 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2932 6443 2932
Size: 3511 2937
BlpI GC'TnA_GC
Cuts at: 3865 3865
Size: 6448
Bme1580I G_kGCm'C
Cuts at: 1790 4509 5665 1790
Size: 2719 1156 2573
Fragments arranged by size:
2719 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6448
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3390 3516 4179
Size: 416 53 83 399 24 1973 126 663
Cuts at: 4179 5137 442
Size: 958 1753
Fragments arranged by size:
1973 1753 958 663 416 399 126 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5108 1239
Size: 3869 2579
Bpu10I CC'TnA_GC
Cuts at: 2328 2328
Size: 6448
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3701 4573 5438 5736 3701
Size: 872 865 298 4413
Fragments arranged by size:
4413 872 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5111 882
Size: 617 3612 2219
Fragments arranged by size:
3612 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4239 493
Size: 711 3035 2702
Fragments arranged by size:
3035 2702 711
BsaBI GATnn'nnATC
Cuts at: 6442 6442
Size: 6448
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4255 4637 5272 275
Size: 53 83 186 936 2722 382 635 1451
Fragments arranged by size:
2722 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2625 4881 5279 5553 5700 6111 2625
Size: 2256 398 274 147 411 2962
Fragments arranged by size:
2962 2256 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6223
Fragments arranged by size:
6223 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2480 1186
Size: 1294 5154
BseYI C'CCAG_C
Cuts at: 2862 5651 6147 2862
Size: 2789 496 3163
Fragments arranged by size:
3163 2789 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2979 1163
Size: 573 1243 4632
Fragments arranged by size:
4632 1243 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3898 4659 5687 1540
Size: 275 186 1897 761 1028 2301
Fragments arranged by size:
2301 1897 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4509 4594 5665 4509
Size: 85 1071 5292
Fragments arranged by size:
5292 1071 85
BsmI GAATG_Cn'
Cuts at: 2918 3959 4052 2918
Size: 1041 93 5314
Fragments arranged by size:
5314 1041 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3767 4509 4594 5665 1391
Size: 399 1977 742 85 1071 2174
Fragments arranged by size:
2174 1977 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2625 6111 2625
Size: 3486 2962
BspHI T'CATG_A
Cuts at: 4337 6067 4337
Size: 1730 4718
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3803 844
Size: 2959 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4335 1394
Size: 429 2512 3507
Fragments arranged by size:
3507 2512 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2670 3512 4943 5117 65
Size: 1183 1422 842 1431 174 1396
Fragments arranged by size:
1431 1422 1396 1183 842 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5092 5279 1700
Size: 110 3282 187 2869
Fragments arranged by size:
3282 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6448
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6448
BssSI C'ACGA_G
Cuts at: 4508 5520 4508
Size: 1012 5436
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6448
BstBI TT'CG_AA
Cuts at: 5252 5252
Size: 6448
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6448
BstYI r'GATC_y
Cuts at: 1972 2932 3337 3778 4145 4530 4547 5999 6085
Size: 960 405 441 367 385 17 1452 86
Cuts at: 6085 6097 6443 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 960 441 405 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5896
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5709
Fragments arranged by size:
5709 683 56
BtsI GCAGTG_nn'
Cuts at: 2202 3964 4758 4778 2202
Size: 1762 794 20 3872
Fragments arranged by size:
3872 1762 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6448
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6413
DraI TTT'AAA
Cuts at: 2421 2635 2688 3752 2421
Size: 214 53 1064 5117
Fragments arranged by size:
5117 1064 214 53
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5455 809
Size: 507 4139 1802
Fragments arranged by size:
4139 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3895 4784 6413
Size: 54 1425 50 275 18 2065 889 1629
Cuts at: 6413 8
Size: 43
Fragments arranged by size:
2065 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3895 1537
Size: 275 2083 4090
Fragments arranged by size:
4090 2083 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2466 4378 1353
Size: 1113 1912 3423
Fragments arranged by size:
3423 1912 1113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5035 5409 5555 387
Size: 1064 138 3446 374 146 1280
Fragments arranged by size:
3446 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2511 4511 5108 5895 6163 1239
Size: 393 879 2000 597 787 268 1524
Fragments arranged by size:
2000 1524 879 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2257 2342 3757 4135 2257
Size: 85 1415 378 4570
Fragments arranged by size:
4570 1415 378 85
FspI TGC'GCA
Cuts at: 4954 4954
Size: 6448
HaeII r_GCGC'y
Cuts at: 1678 5595 1678
Size: 3917 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2659 2691 5089 5121 5163
Size: 32 135 32 1087 32 2398 32 42
Cuts at: 5163 5195 1373
Size: 32 2626
Fragments arranged by size:
2626 2398 1087 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3792 4038 669
Size: 988 281 1854 246 3079
Fragments arranged by size:
3079 1854 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3223 748
Size: 2475 3973
HpaI GTT'AAC
Cuts at: 4038 4038
Size: 6448
KpnI G_GTAC'C
Cuts at: 3773 3773
Size: 6448
MfeI C'AATT_G
Cuts at: 4047 4047
Size: 6448
MluI A'CGCG_T
Cuts at: 6324 6324
Size: 6448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2702 5562 5746 1305
Size: 1397 2860 184 2007
Fragments arranged by size:
2860 2007 1397 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4626
Fragments arranged by size:
4626 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4406 4765 4924 518
Size: 648 3240 359 159 2042
Fragments arranged by size:
3240 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3864 4541 5689 5934 1606
Size: 2258 677 1148 245 2120
Fragments arranged by size:
2258 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5896
NdeI CA'TA_TG
Cuts at: 387 3478 387
Size: 3091 3357
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6448
NotI GC'GGCC_GC
Cuts at: 3895 3895
Size: 6448
NspI r_CATG'y
Cuts at: 1132 1229 2051 2085 2336 2663 3144 3806 5351
Size: 97 822 34 251 327 481 662 1545
Cuts at: 5351 1132
Size: 2229
Fragments arranged by size:
2229 1545 822 662 481 327 251 97
34
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6448
PciI A'CATG_T
Cuts at: 3140 5347 3140
Size: 2207 4241
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6448
PmeI GTTT'AAAC
Cuts at: 3752 3752
Size: 6448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4517 4549 6030 6062 4517
Size: 32 1481 32 4903
Fragments arranged by size:
4903 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6448
PsiI TTA'TAA
Cuts at: 4018 4018
Size: 6448
PstI C_TGCA'G
Cuts at: 830 2179 3800 830
Size: 1349 1621 3478
Fragments arranged by size:
3478 1621 1349
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6448
SalI G'TCGA_C
Cuts at: 1936 3790 1936
Size: 1854 4594
SbfI CC_TGCA'GG
Cuts at: 3800 3800
Size: 6448
ScaI AGT'ACT
Cuts at: 4696 6408 4696
Size: 1712 4736
SfcI C'TryA_G
Cuts at: 826 1045 2175 3126 3796 4931 5612 5803 826
Size: 219 1130 951 670 1135 681 191 1471
Fragments arranged by size:
1471 1135 1130 951 681 670 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6448
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6448
SmaI CCC'GGG
Cuts at: 1872 3775 1872
Size: 1903 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3680 4552 5453 5715 820
Size: 197 926 1737 872 901 262 1553
Fragments arranged by size:
1737 1553 926 901 872 262 197
SnaBI TAC'GTA
Cuts at: 493 4239 493
Size: 3746 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6448
SphI G_CATG'C
Cuts at: 1132 2051 3806 1132
Size: 919 1755 3774
Fragments arranged by size:
3774 1755 919
SspI AAT'ATT
Cuts at: 5 52 4372 5
Size: 47 4320 2081
Fragments arranged by size:
4320 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6448
StyI C'CwwG_G
Cuts at: 513 1065 3887 513
Size: 552 2822 3074
Fragments arranged by size:
3074 2822 552
SwaI ATTT'AAAT
Cuts at: 2688 2688
Size: 6448
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4475 4492 4645 4830 5275 325
Size: 1255 2895 17 153 185 445 1498
Fragments arranged by size:
2895 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4694 6406 96
Size: 274 80 33 51 1443 2717 1712 138
Fragments arranged by size:
2717 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6408 6440 6408
Size: 32 6416
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6448
XbaI T'CTAG_A
Cuts at: 3784 3784
Size: 6448
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6448
XmaI C'CCGG_G
Cuts at: 1870 3773 1870
Size: 1903 4545
XmnI GAAnn'nnTTC
Cuts at: 2934 3012 4577 2934
Size: 78 1565 4805
Fragments arranged by size:
4805 1565 78
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4256 276
Size: 53 83 186 936 2722 2468
Fragments arranged by size:
2722 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI