Restriction map for
pFN21AE0375
(Circular) (Six-base) MAPSORT of: pFN21AE0375.seq Check: 5018 from: 1 to: 6406
pFN21AE0375 6406 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2371 2371
Size: 6406
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2208 4216 278
Size: 53 83 186 936 672 2008 2468
Fragments arranged by size:
2468 2008 936 672 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3359 3749 1937
Size: 1422 390 4594
Fragments arranged by size:
4594 1422 390
Acc65I G'GTAC_C
Cuts at: 3540 3634 3727 3540
Size: 94 93 6219
Fragments arranged by size:
6219 94 93
AclI AA'CG_TT
Cuts at: 1563 4533 4906 1563
Size: 2970 373 3063
Fragments arranged by size:
3063 2970 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2521 2681 3456 4469 5853 6121 1632
Size: 889 160 775 1013 1384 268 1917
Fragments arranged by size:
1917 1384 1013 889 775 268 160
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6209
AflIII A'CryG_T
Cuts at: 1203 5305 6282 1203
Size: 4102 977 1327
Fragments arranged by size:
4102 1327 977
AgeI A'CCGG_T
Cuts at: 2968 5237 2968
Size: 2269 4137
AhdI GACnn_n'nnGTC
Cuts at: 5135 5135
Size: 6406
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6406
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6406
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3053 3085 3053
Size: 32 6374
AlwNI CAG_nnn'CTG
Cuts at: 4149 5721 4149
Size: 1572 4834
ApaI G_GGCC'C
Cuts at: 3084 3084
Size: 6406
ApaLI G'TGCA_C
Cuts at: 4463 5619 4463
Size: 1156 5250
ApoI r'AATT_y
Cuts at: 1493 2402 2775 3254 3288 3434 3715 3941 4093
Size: 909 373 479 34 146 281 226 152
Cuts at: 4093 6202 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 909 479 373 281 226 152
146 34
AseI AT'TA_AT
Cuts at: 160 1032 4960 160
Size: 872 3928 1606
Fragments arranged by size:
3928 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
AvaI C'yCGr_G
Cuts at: 1870 1943 2610 3731 1870
Size: 73 667 1121 4545
Fragments arranged by size:
4545 1121 667 73
BamHI G'GATC_C
Cuts at: 3736 4103 3736
Size: 367 6039
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2050 2165 2672 3540 3634
Size: 325 217 627 263 115 507 868 94
Cuts at: 3634 3727 5182 618
Size: 93 1455 1842
Fragments arranged by size:
1842 1455 868 627 507 325 263 217
115 94 93
BanII G_rGCy'C
Cuts at: 1391 3084 3597 3725 1391
Size: 1693 513 128 4072
Fragments arranged by size:
4072 1693 513 128
BbeI G_GCGC'C
Cuts at: 2169 2169
Size: 6406
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2200 928
Size: 971 301 5134
Fragments arranged by size:
5134 971 301
BbvCI CC'TCA_GC
Cuts at: 2303 2303
Size: 6406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4597 4631 1903
Size: 34 2660 34 3678
Fragments arranged by size:
3678 2660 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3299 3395 4298 5508 3299
Size: 96 903 1210 4197
Fragments arranged by size:
4197 1210 903 96
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5017 136
Size: 107 122 71 631 605 3345 1525
Fragments arranged by size:
3345 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3136 6401 3136
Size: 3265 3141
BlpI GC'TnA_GC
Cuts at: 3823 3823
Size: 6406
Bme1580I G_kGCm'C
Cuts at: 1790 2055 3084 4467 5623 1790
Size: 265 1029 1383 1156 2573
Fragments arranged by size:
2573 1383 1156 1029 265
BmgBI CAC'GTC
Cuts at: 1318 2317 1318
Size: 999 5407
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2479 2803 4137
Size: 416 53 83 399 24 1062 324 1334
Cuts at: 4137 5095 442
Size: 958 1753
Fragments arranged by size:
1753 1334 1062 958 416 399 324 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2474 2966 2972 5066 1239
Size: 1235 492 6 2094 2579
Fragments arranged by size:
2579 2094 1235 492 6
Bpu10I CC'TnA_GC
Cuts at: 2303 2303
Size: 6406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4531 5396 5694 4531
Size: 865 298 5243
Fragments arranged by size:
5243 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3375 5069 882
Size: 617 1876 1694 2219
Fragments arranged by size:
2219 1876 1694 617
BsaAI yAC'GTr
Cuts at: 493 1204 2745 4197 493
Size: 711 1541 1452 2702
Fragments arranged by size:
2702 1541 1452 711
BsaBI GATnn'nnATC
Cuts at: 6400 6400
Size: 6406
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2166 2205 4213 4595
Size: 53 83 186 936 633 39 2008 382
Cuts at: 4595 5230 275
Size: 635 1451
Fragments arranged by size:
2008 1451 936 635 633 382 186 83
53 39
BsaWI w'CCGG_w
Cuts at: 2968 4839 5237 5511 5658 6069 2968
Size: 1871 398 274 147 411 3305
Fragments arranged by size:
3305 1871 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2318 2348 2472 2502 3053
Size: 30 165 30 915 30 124 30 551
Cuts at: 3053 3083 3669 3699 1178
Size: 30 586 30 3885
Fragments arranged by size:
3885 915 586 551 165 124 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3677 3709 1186
Size: 2491 32 3883
Fragments arranged by size:
3883 2491 32
BseYI C'CCAG_C
Cuts at: 2059 3083 5609 6105 2059
Size: 1024 2526 496 2360
Fragments arranged by size:
2526 2360 1024 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2297 2958 1163
Size: 573 561 661 4611
Fragments arranged by size:
4611 661 573 561
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3100 3856 4617 5645 1540
Size: 275 186 1099 756 761 1028 2301
Fragments arranged by size:
2301 1099 1028 761 756 275 186
BsiHKAI G_wGCw'C
Cuts at: 4467 4552 5623 4467
Size: 85 1071 5250
Fragments arranged by size:
5250 1071 85
BsmI GAATG_Cn'
Cuts at: 2728 3917 4010 2728
Size: 1189 93 5124
Fragments arranged by size:
5124 1189 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2240 2630 2240
Size: 390 6016
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 3084 3597 3725 4467 4552 5623
Size: 399 265 1029 513 128 742 85 1071
Cuts at: 5623 1391
Size: 2174
Fragments arranged by size:
2174 1071 1029 742 513 399 265 128
85
BspEI T'CCGG_A
Cuts at: 6069 6069
Size: 6406
BspHI T'CATG_A
Cuts at: 2910 4295 6025 2910
Size: 1385 1730 3291
Fragments arranged by size:
3291 1730 1385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2371 3761 844
Size: 1527 1390 3489
Fragments arranged by size:
3489 1527 1390
BsrBI CCG'CTC
Cuts at: 1394 1823 4293 1394
Size: 429 2470 3507
Fragments arranged by size:
3507 2470 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2622 3498 4901 5075 65
Size: 1183 1374 876 1403 174 1396
Fragments arranged by size:
1403 1396 1374 1183 876 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2675 2891 2968 5050 5237 1700
Size: 110 865 216 77 2082 187 2869
Fragments arranged by size:
2869 2082 865 216 187 110 77
BsrGI T'GTAC_A
Cuts at: 96 2682 96
Size: 2586 3820
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6406
BssSI C'ACGA_G
Cuts at: 4466 5478 4466
Size: 1012 5394
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6406
BstBI TT'CG_AA
Cuts at: 5210 5210
Size: 6406
BstEII G'GTnAC_C
Cuts at: 1184 2964 1184
Size: 1780 4626
BstYI r'GATC_y
Cuts at: 1972 2473 3136 3736 4103 4488 4505 5957 6043
Size: 501 663 600 367 385 17 1452 86
Cuts at: 6043 6055 6401 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 663 600 501 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3360 3360
Size: 6406
Bsu36I CC'TnA_GG
Cuts at: 3692 3692
Size: 6406
BtgI C'CryG_G
Cuts at: 513 1065 2668 3167 513
Size: 552 1603 499 3752
Fragments arranged by size:
3752 1603 552 499
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5667
Fragments arranged by size:
5667 683 56
BtsI GCAGTG_nn'
Cuts at: 3666 3922 4716 4736 3666
Size: 256 794 20 5336
Fragments arranged by size:
5336 794 256 20
ClaI AT'CG_AT
Cuts at: 1554 2421 1554
Size: 867 5539
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2485 2520 3510 3545 529
Size: 35 1921 35 990 35 3390
Fragments arranged by size:
3390 1921 990 35 35 35
DraI TTT'AAA
Cuts at: 3710 3710
Size: 6406
DraIII CAC_nnn'GTG
Cuts at: 2219 2745 2219
Size: 526 5880
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5413 809
Size: 507 4097 1802
Fragments arranged by size:
4097 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2526 3131 3853
Size: 54 1425 50 275 18 696 605 722
Cuts at: 3853 4742 6371 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 722 696 605 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3853 1537
Size: 275 2041 4090
Fragments arranged by size:
4090 2041 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 2984 4336 1353
Size: 729 902 1352 3423
Fragments arranged by size:
3423 1352 902 729
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4993 5367 5513 387
Size: 1064 138 3404 374 146 1280
Fragments arranged by size:
3404 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2474 2521 2681 2966 2972 3456 4469
Size: 393 842 47 160 285 6 484 1013
Cuts at: 4469 5066 5853 6121 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1013 842 787 597 484 393 285
268 160 47 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2684 2684
Size: 6406
EcoO109I rG'GnC_Cy
Cuts at: 2079 2189 2827 3080 2079
Size: 110 638 253 5405
Fragments arranged by size:
5405 638 253 110
EcoRI G'AATT_C
Cuts at: 2402 3288 3715 4093 2402
Size: 886 427 378 4715
Fragments arranged by size:
4715 886 427 378
FspI TGC'GCA
Cuts at: 4912 4912
Size: 6406
HaeII r_GCGC'y
Cuts at: 1678 2169 5553 1678
Size: 491 3384 2531
Fragments arranged by size:
3384 2531 491
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3128 3160 5047 5079 5121
Size: 32 135 32 1556 32 1887 32 42
Cuts at: 5121 5153 1373
Size: 32 2626
Fragments arranged by size:
2626 1887 1556 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2320 3121 3750 3996 669
Size: 988 281 382 801 629 246 3079
Fragments arranged by size:
3079 988 801 629 382 281 246
HindIII A'AGCT_T
Cuts at: 748 3549 748
Size: 2801 3605
HpaI GTT'AAC
Cuts at: 3996 3996
Size: 6406
KasI G'GCGC_C
Cuts at: 2165 2165
Size: 6406
KpnI G_GTAC'C
Cuts at: 3544 3638 3731 3544
Size: 94 93 6219
Fragments arranged by size:
6219 94 93
MfeI C'AATT_G
Cuts at: 4005 4005
Size: 6406
MluI A'CGCG_T
Cuts at: 6282 6282
Size: 6406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3357 5520 5704 1305
Size: 2052 2163 184 2007
Fragments arranged by size:
2163 2052 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2528 3133 10
Size: 54 1425 343 696 605 3283
Fragments arranged by size:
3283 1425 696 605 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3227 3614 4364 4723 4882 518
Size: 648 2061 387 750 359 159 2042
Fragments arranged by size:
2061 2042 750 648 387 359 159
MspA1I CmG'CkG
Cuts at: 1606 2485 2670 3822 4499 5647 5892 1606
Size: 879 185 1152 677 1148 245 2120
Fragments arranged by size:
2120 1152 1148 879 677 245 185
NarI GG'CG_CC
Cuts at: 2166 2166
Size: 6406
NcoI C'CATG_G
Cuts at: 513 1065 3167 513
Size: 552 2102 3752
Fragments arranged by size:
3752 2102 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6406
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6406
NotI GC'GGCC_GC
Cuts at: 3853 3853
Size: 6406
NspI r_CATG'y
Cuts at: 1132 1229 3613 3764 5309 1132
Size: 97 2384 151 1545 2229
Fragments arranged by size:
2384 2229 1545 151 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6406
PciI A'CATG_T
Cuts at: 5305 5305
Size: 6406
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2724 1108
Size: 1616 4790
PfoI T'CCnGG_A
Cuts at: 2283 2982 2283
Size: 699 5707
PmeI GTTT'AAAC
Cuts at: 3710 3710
Size: 6406
PmlI CAC'GTG
Cuts at: 2745 2745
Size: 6406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2101 2133 3053 3085 4475 4507 5988 6020 2101
Size: 32 920 32 1390 32 1481 32 2487
Fragments arranged by size:
2487 1481 1390 920 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2079 2827 2079
Size: 748 5658
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6406
PsiI TTA'TAA
Cuts at: 2736 3976 2736
Size: 1240 5166
PspOMI G'GGCC_C
Cuts at: 3080 3080
Size: 6406
PstI C_TGCA'G
Cuts at: 830 3592 3758 830
Size: 2762 166 3478
Fragments arranged by size:
3478 2762 166
PvuI CG_AT'CG
Cuts at: 2001 3100 2001
Size: 1099 5307
PvuII CAG'CTG
Cuts at: 2485 2485
Size: 6406
SacII CC_GC'GG
Cuts at: 2671 2671
Size: 6406
SalI G'TCGA_C
Cuts at: 1936 3748 1936
Size: 1812 4594
SanDI GG'GwC_CC
Cuts at: 2079 2079
Size: 6406
SapI GCTCTTCn'nnn_
Cuts at: 2984 2984
Size: 6406
SbfI CC_TGCA'GG
Cuts at: 3758 3758
Size: 6406
ScaI AGT'ACT
Cuts at: 4654 6366 4654
Size: 1712 4694
SfcI C'TryA_G
Cuts at: 826 1045 2407 2852 3588 3754 4889 5570 5761
Size: 219 1362 445 736 166 1135 681 191
Cuts at: 5761 826
Size: 1471
Fragments arranged by size:
1471 1362 1135 736 681 445 219 191
166
SfoI GGC'GCC
Cuts at: 2167 2167
Size: 6406
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6406
SmaI CCC'GGG
Cuts at: 1872 3733 1872
Size: 1861 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4510 5411 5673 820
Size: 197 926 2567 901 262 1553
Fragments arranged by size:
2567 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4197 493
Size: 3704 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6406
SphI G_CATG'C
Cuts at: 1132 3764 1132
Size: 2632 3774
SspI AAT'ATT
Cuts at: 5 52 3482 4330 5
Size: 47 3430 848 2081
Fragments arranged by size:
3430 2081 848 47
StuI AGG'CCT
Cuts at: 1517 2093 2567 2949 1517
Size: 576 474 382 4974
Fragments arranged by size:
4974 576 474 382
StyI C'CwwG_G
Cuts at: 513 1065 2823 3167 3845 513
Size: 552 1758 344 678 3074
Fragments arranged by size:
3074 1758 678 552 344
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2496 4433 4450 4603 4788 5233 325
Size: 1255 916 1937 17 153 185 445 1498
Fragments arranged by size:
1937 1498 1255 916 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2682 3263 4652
Size: 274 80 33 51 1443 705 581 1389
Cuts at: 4652 6364 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1389 705 581 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2472 2504 6366 6398 2472
Size: 32 3862 32 2480
Fragments arranged by size:
3862 2480 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6406
XbaI T'CTAG_A
Cuts at: 3742 3742
Size: 6406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3229 3229
Size: 6406
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6406
XmaI C'CCGG_G
Cuts at: 1870 3731 1870
Size: 1861 4545
XmnI GAAnn'nnTTC
Cuts at: 4535 4535
Size: 6406
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2206 4214 276
Size: 53 83 186 936 672 2008 2468
Fragments arranged by size:
2468 2008 936 672 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BplI BsiWI BstXI
EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PspXI PsrI RsrII SacI SexAI SfiI
SrfI SwaI