(Circular) (Six-base) MAPSORT of: pFN21AE0374.seq Check: 9484 from: 1 to: 7045 pFN21AE0374 7045 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2268 4855 278 Size: 53 83 186 936 732 2587 2468 Fragments arranged by size: 2587 2468 936 732 186 83 53 AccI GT'mk_AC Cuts at: 1937 2082 4388 1937 Size: 145 2306 4594 Fragments arranged by size: 4594 2306 145 Acc65I G'GTAC_C Cuts at: 4366 4366 Size: 7045 AclI AA'CG_TT Cuts at: 1563 3951 5172 5545 1563 Size: 2388 1221 373 3063 Fragments arranged by size: 3063 2388 1221 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5108 6492 6760 1632 Size: 3476 1384 268 1917 Fragments arranged by size: 3476 1917 1384 268 AfeI AGC'GCT Cuts at: 3756 3756 Size: 7045 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6848 AflIII A'CryG_T Cuts at: 1203 3458 5944 6921 1203 Size: 2255 2486 977 1327 Fragments arranged by size: 2486 2255 1327 977 AgeI A'CCGG_T Cuts at: 5876 5876 Size: 7045 AhdI GACnn_n'nnGTC Cuts at: 5774 5774 Size: 7045 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7045 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2156 1184 Size: 972 6073 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2442 2474 3873 3905 4296 4328 2442 Size: 32 1399 32 391 32 5159 Fragments arranged by size: 5159 1399 391 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2339 2684 4788 6360 2339 Size: 345 2104 1572 3024 Fragments arranged by size: 3024 2104 1572 345 ApaLI G'TGCA_C Cuts at: 5102 6258 5102 Size: 1156 5889 ApoI r'AATT_y Cuts at: 1493 3155 4049 4354 4580 4732 6841 1493 Size: 1662 894 305 226 152 2109 1697 Fragments arranged by size: 2109 1697 1662 894 305 226 152 AseI AT'TA_AT Cuts at: 160 1032 4277 5599 160 Size: 872 3245 1322 1606 Fragments arranged by size: 3245 1606 1322 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7045 AvaI C'yCGr_G Cuts at: 1870 1943 2045 4370 1870 Size: 73 102 2325 4545 Fragments arranged by size: 4545 2325 102 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3583 3616 3583 Size: 33 7012 BamHI G'GATC_C Cuts at: 4375 4742 4375 Size: 367 6678 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2118 4366 5821 618 Size: 325 217 627 331 2248 1455 1842 Fragments arranged by size: 2248 1842 1455 627 331 325 217 BanII G_rGCy'C Cuts at: 1391 2094 4364 1391 Size: 703 2270 4072 Fragments arranged by size: 4072 2270 703 BbeI G_GCGC'C Cuts at: 2122 2122 Size: 7045 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6074 BbvCI CC'TCA_GC Cuts at: 3758 3758 Size: 7045 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5236 5270 1903 Size: 34 3299 34 3678 Fragments arranged by size: 3678 3299 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2503 4937 6147 2503 Size: 2434 1210 3401 Fragments arranged by size: 3401 2434 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7045 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5656 136 Size: 107 122 71 631 605 3984 1525 Fragments arranged by size: 3984 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3871 7040 3871 Size: 3169 3876 BlpI GC'TnA_GC Cuts at: 4462 4462 Size: 7045 Bme1580I G_kGCm'C Cuts at: 1790 2214 2578 5106 6262 1790 Size: 424 364 2528 1156 2573 Fragments arranged by size: 2573 2528 1156 424 364 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7045 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2295 2888 4776 Size: 416 53 83 399 24 878 593 1888 Cuts at: 4776 5734 442 Size: 958 1753 Fragments arranged by size: 1888 1753 958 878 593 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7045 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3273 3896 4133 5705 1239 Size: 2034 623 237 1572 2579 Fragments arranged by size: 2579 2034 1572 623 237 Bpu10I CC'TnA_GC Cuts at: 3758 3758 Size: 7045 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3615 4194 5170 6035 6333 3615 Size: 579 976 865 298 4327 Fragments arranged by size: 4327 976 865 579 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2357 2707 5708 882 Size: 617 858 350 3001 2219 Fragments arranged by size: 3001 2219 858 617 350 BsaAI yAC'GTr Cuts at: 493 1204 3459 4836 493 Size: 711 2255 1377 2702 Fragments arranged by size: 2702 2255 1377 711 BsaBI GATnn'nnATC Cuts at: 2805 7039 2805 Size: 4234 2811 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2119 2265 4852 5234 Size: 53 83 186 936 586 146 2587 382 Cuts at: 5234 5869 275 Size: 635 1451 Fragments arranged by size: 2587 1451 936 635 586 382 186 146 83 53 BsaWI w'CCGG_w Cuts at: 5478 5876 6150 6297 6708 5478 Size: 398 274 147 411 5815 Fragments arranged by size: 5815 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6820 Fragments arranged by size: 6820 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7045 BseYI C'CCAG_C Cuts at: 6248 6744 6248 Size: 496 6549 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6472 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2078 3237 4495 5256 6284 1540 Size: 275 186 77 1159 1258 761 1028 2301 Fragments arranged by size: 2301 1258 1159 1028 761 275 186 77 BsiHKAI G_wGCw'C Cuts at: 2094 3251 5106 5191 6262 2094 Size: 1157 1855 85 1071 2877 Fragments arranged by size: 2877 1855 1157 1071 85 BsmI GAATG_Cn' Cuts at: 4556 4649 4556 Size: 93 6952 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2214 2578 3251 4364 5106 5191 Size: 399 304 120 364 673 1113 742 85 Cuts at: 5191 6262 1391 Size: 1071 2174 Fragments arranged by size: 2174 1113 1071 742 673 399 364 304 120 85 BspEI T'CCGG_A Cuts at: 6708 6708 Size: 7045 BspHI T'CATG_A Cuts at: 4934 6664 4934 Size: 1730 5315 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2013 4400 844 Size: 1169 2387 3489 Fragments arranged by size: 3489 2387 1169 BsrBI CCG'CTC Cuts at: 1394 1823 2116 4932 1394 Size: 429 293 2816 3507 Fragments arranged by size: 3507 2816 429 293 BsrDI GCAATG_nn' Cuts at: 65 1248 2509 3159 3682 4124 5540 5714 65 Size: 1183 1261 650 523 442 1416 174 1396 Fragments arranged by size: 1416 1396 1261 1183 650 523 442 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2077 5689 5876 1700 Size: 110 267 3612 187 2869 Fragments arranged by size: 3612 2869 267 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7045 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7045 BssSI C'ACGA_G Cuts at: 2111 5105 6117 2111 Size: 2994 1012 3039 Fragments arranged by size: 3039 2994 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7045 BstBI TT'CG_AA Cuts at: 5849 5849 Size: 7045 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7045 BstXI CCAn_nnnn'nTGG Cuts at: 3076 3478 3076 Size: 402 6643 BstYI r'GATC_y Cuts at: 1972 2450 2653 3487 3871 4375 4742 5127 5144 Size: 478 203 834 384 504 367 385 17 Cuts at: 5144 6596 6682 6694 7040 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 834 504 478 385 384 367 346 203 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5554 Fragments arranged by size: 5554 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3055 487 Size: 683 56 1829 4477 Fragments arranged by size: 4477 1829 683 56 BtsI GCAGTG_nn' Cuts at: 2535 2820 3102 3216 4028 4561 5355 5375 2535 Size: 285 282 114 812 533 794 20 4205 Fragments arranged by size: 4205 812 794 533 285 282 114 20 ClaI AT'CG_AT Cuts at: 1554 3141 1554 Size: 1587 5458 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7010 DraI TTT'AAA Cuts at: 3691 4349 3691 Size: 658 6387 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6052 809 Size: 507 4736 1802 Fragments arranged by size: 4736 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2075 2154 2295 Size: 54 1425 50 275 18 245 79 141 Cuts at: 2295 3210 3234 4492 5381 7010 8 Size: 915 24 1258 889 1629 43 Fragments arranged by size: 1629 1425 1258 915 889 275 245 141 79 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2075 3234 4492 1537 Size: 275 263 1159 1258 4090 Fragments arranged by size: 4090 1258 1159 275 263 EarI CTCTTCn'nnn_ Cuts at: 1353 4975 1353 Size: 3622 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5632 6006 6152 387 Size: 1064 138 4043 374 146 1280 Fragments arranged by size: 4043 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2092 2092 Size: 7045 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3273 3896 4133 5108 5705 6492 6760 Size: 393 1641 623 237 975 597 787 268 Cuts at: 6760 1239 Size: 1524 Fragments arranged by size: 1641 1524 975 787 623 597 393 268 237 EcoRI G'AATT_C Cuts at: 3155 4049 4354 4732 3155 Size: 894 305 378 5468 Fragments arranged by size: 5468 894 378 305 EcoRV GAT'ATC Cuts at: 3589 3889 3589 Size: 300 6745 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2203 3367 4286 4322 2203 Size: 1164 919 36 4926 Fragments arranged by size: 4926 1164 919 36 FspI TGC'GCA Cuts at: 5551 5551 Size: 7045 HaeII r_GCGC'y Cuts at: 1678 2015 2122 3758 6192 1678 Size: 337 107 1636 2434 2531 Fragments arranged by size: 2531 2434 1636 337 107 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2337 2369 2874 2906 3911 Size: 32 135 32 765 32 505 32 1005 Cuts at: 3911 3943 5686 5718 5760 5792 1373 Size: 32 1743 32 42 32 2626 Fragments arranged by size: 2626 1743 1005 765 505 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3525 4389 4635 669 Size: 988 281 1587 864 246 3079 Fragments arranged by size: 3079 1587 988 864 281 246 HindIII A'AGCT_T Cuts at: 748 3323 748 Size: 2575 4470 HpaI GTT'AAC Cuts at: 4635 4635 Size: 7045 KasI G'GCGC_C Cuts at: 2118 2118 Size: 7045 KpnI G_GTAC'C Cuts at: 4370 4370 Size: 7045 MfeI C'AATT_G Cuts at: 4644 4644 Size: 7045 MluI A'CGCG_T Cuts at: 6921 6921 Size: 7045 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2007 2323 2398 2572 3694 3997 6159 6343 Size: 702 316 75 174 1122 303 2162 184 Cuts at: 6343 1305 Size: 2007 Fragments arranged by size: 2162 2007 1122 702 316 303 184 174 75 MscI TGG'CCA Cuts at: 10 64 1489 1832 2156 2297 3212 10 Size: 54 1425 343 324 141 915 3843 Fragments arranged by size: 3843 1425 915 343 324 141 54 MslI CAynn'nnrTG Cuts at: 518 1166 3074 5003 5362 5521 518 Size: 648 1908 1929 359 159 2042 Fragments arranged by size: 2042 1929 1908 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2055 2681 3359 3397 4461 5138 6286 6531 Size: 449 626 678 38 1064 677 1148 245 Cuts at: 6531 1606 Size: 2120 Fragments arranged by size: 2120 1148 1064 678 677 626 449 245 38 NarI GG'CG_CC Cuts at: 2119 2119 Size: 7045 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5554 Fragments arranged by size: 5554 939 552 NdeI CA'TA_TG Cuts at: 387 2317 2947 387 Size: 1930 630 4485 Fragments arranged by size: 4485 1930 630 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7045 NotI GC'GGCC_GC Cuts at: 4492 4492 Size: 7045 NspI r_CATG'y Cuts at: 1132 1229 4403 5948 1132 Size: 97 3174 1545 2229 Fragments arranged by size: 3174 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3316 1348 Size: 1968 5077 PciI A'CATG_T Cuts at: 5944 5944 Size: 7045 PflMI CCAn_nnn'nTGG Cuts at: 1108 2698 1108 Size: 1590 5455 PfoI T'CCnGG_A Cuts at: 3240 4005 4314 3240 Size: 765 309 5971 Fragments arranged by size: 5971 765 309 PmeI GTTT'AAAC Cuts at: 4349 4349 Size: 7045 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2472 2504 5114 5146 6627 6659 2472 Size: 32 2610 32 1481 32 2858 Fragments arranged by size: 2858 2610 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3530 1613 Size: 1917 5128 PsiI TTA'TAA Cuts at: 4615 4615 Size: 7045 PstI C_TGCA'G Cuts at: 830 2060 2127 2168 2335 2542 4397 830 Size: 1230 67 41 167 207 1855 3478 Fragments arranged by size: 3478 1855 1230 207 167 67 41 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7045 PvuII CAG'CTG Cuts at: 2055 2681 3359 3397 2055 Size: 626 678 38 5703 Fragments arranged by size: 5703 678 626 38 SacI G_AGCT'C Cuts at: 2094 2094 Size: 7045 SalI G'TCGA_C Cuts at: 1936 4387 1936 Size: 2451 4594 SbfI CC_TGCA'GG Cuts at: 2127 4397 2127 Size: 2270 4775 ScaI AGT'ACT Cuts at: 2284 5293 7005 2284 Size: 3009 1712 2324 Fragments arranged by size: 3009 2324 1712 SfcI C'TryA_G Cuts at: 826 1045 2056 2083 2123 2164 2331 2538 2907 Size: 219 1011 27 40 41 167 207 369 Cuts at: 2907 4190 4393 5528 6209 6400 826 Size: 1283 203 1135 681 191 1471 Fragments arranged by size: 1471 1283 1135 1011 681 369 219 207 203 191 167 41 40 27 SfoI GGC'GCC Cuts at: 2120 2120 Size: 7045 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7045 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7045 SmaI CCC'GGG Cuts at: 1872 4372 1872 Size: 2500 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3630 4209 5149 6050 6312 820 Size: 197 926 1687 579 940 901 262 1553 Fragments arranged by size: 1687 1553 940 926 901 579 262 197 SnaBI TAC'GTA Cuts at: 493 4836 493 Size: 4343 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7045 SphI G_CATG'C Cuts at: 1132 4403 1132 Size: 3271 3774 SspI AAT'ATT Cuts at: 5 52 2794 4969 5 Size: 47 2742 2175 2081 Fragments arranged by size: 2742 2175 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7045 StyI C'CwwG_G Cuts at: 513 1065 2004 2999 3565 3897 4484 513 Size: 552 939 995 566 332 587 3074 Fragments arranged by size: 3074 995 939 587 566 552 332 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3337 3439 5072 5089 5242 5427 5872 Size: 1255 1757 102 1633 17 153 185 445 Cuts at: 5872 325 Size: 1498 Fragments arranged by size: 1757 1633 1498 1255 445 185 153 102 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2049 2282 5291 Size: 274 80 33 51 1443 72 233 3009 Cuts at: 5291 7003 96 Size: 1712 138 Fragments arranged by size: 3009 1712 1443 274 233 138 80 72 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2622 2654 7005 7037 2622 Size: 32 4351 32 2630 Fragments arranged by size: 4351 2630 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7045 XbaI T'CTAG_A Cuts at: 4381 4381 Size: 7045 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3076 3248 3313 3076 Size: 172 65 6808 Fragments arranged by size: 6808 172 65 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7045 XmaI C'CCGG_G Cuts at: 1870 4370 1870 Size: 2500 4545 XmnI GAAnn'nnTTC Cuts at: 2484 5174 2484 Size: 2690 4355 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2266 4853 276 Size: 53 83 186 936 732 2587 2468 Fragments arranged by size: 2587 2468 936 732 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI