Restriction map for
pFN21AE0374
(Circular) (Six-base) MAPSORT of: pFN21AE0374.seq Check: 9484 from: 1 to: 7045
pFN21AE0374 7045 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2268 4855 278
Size: 53 83 186 936 732 2587 2468
Fragments arranged by size:
2587 2468 936 732 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2082 4388 1937
Size: 145 2306 4594
Fragments arranged by size:
4594 2306 145
Acc65I G'GTAC_C
Cuts at: 4366 4366
Size: 7045
AclI AA'CG_TT
Cuts at: 1563 3951 5172 5545 1563
Size: 2388 1221 373 3063
Fragments arranged by size:
3063 2388 1221 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5108 6492 6760 1632
Size: 3476 1384 268 1917
Fragments arranged by size:
3476 1917 1384 268
AfeI AGC'GCT
Cuts at: 3756 3756
Size: 7045
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6848
AflIII A'CryG_T
Cuts at: 1203 3458 5944 6921 1203
Size: 2255 2486 977 1327
Fragments arranged by size:
2486 2255 1327 977
AgeI A'CCGG_T
Cuts at: 5876 5876
Size: 7045
AhdI GACnn_n'nnGTC
Cuts at: 5774 5774
Size: 7045
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7045
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2156 1184
Size: 972 6073
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2442 2474 3873 3905 4296 4328 2442
Size: 32 1399 32 391 32 5159
Fragments arranged by size:
5159 1399 391 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2339 2684 4788 6360 2339
Size: 345 2104 1572 3024
Fragments arranged by size:
3024 2104 1572 345
ApaLI G'TGCA_C
Cuts at: 5102 6258 5102
Size: 1156 5889
ApoI r'AATT_y
Cuts at: 1493 3155 4049 4354 4580 4732 6841 1493
Size: 1662 894 305 226 152 2109 1697
Fragments arranged by size:
2109 1697 1662 894 305 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4277 5599 160
Size: 872 3245 1322 1606
Fragments arranged by size:
3245 1606 1322 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7045
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 4370 1870
Size: 73 102 2325 4545
Fragments arranged by size:
4545 2325 102 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3583 3616 3583
Size: 33 7012
BamHI G'GATC_C
Cuts at: 4375 4742 4375
Size: 367 6678
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2118 4366 5821 618
Size: 325 217 627 331 2248 1455 1842
Fragments arranged by size:
2248 1842 1455 627 331 325 217
BanII G_rGCy'C
Cuts at: 1391 2094 4364 1391
Size: 703 2270 4072
Fragments arranged by size:
4072 2270 703
BbeI G_GCGC'C
Cuts at: 2122 2122
Size: 7045
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6074
BbvCI CC'TCA_GC
Cuts at: 3758 3758
Size: 7045
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5236 5270 1903
Size: 34 3299 34 3678
Fragments arranged by size:
3678 3299 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2503 4937 6147 2503
Size: 2434 1210 3401
Fragments arranged by size:
3401 2434 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7045
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5656 136
Size: 107 122 71 631 605 3984 1525
Fragments arranged by size:
3984 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3871 7040 3871
Size: 3169 3876
BlpI GC'TnA_GC
Cuts at: 4462 4462
Size: 7045
Bme1580I G_kGCm'C
Cuts at: 1790 2214 2578 5106 6262 1790
Size: 424 364 2528 1156 2573
Fragments arranged by size:
2573 2528 1156 424 364
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7045
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2295 2888 4776
Size: 416 53 83 399 24 878 593 1888
Cuts at: 4776 5734 442
Size: 958 1753
Fragments arranged by size:
1888 1753 958 878 593 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7045
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3273 3896 4133 5705 1239
Size: 2034 623 237 1572 2579
Fragments arranged by size:
2579 2034 1572 623 237
Bpu10I CC'TnA_GC
Cuts at: 3758 3758
Size: 7045
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3615 4194 5170 6035 6333 3615
Size: 579 976 865 298 4327
Fragments arranged by size:
4327 976 865 579 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2357 2707 5708 882
Size: 617 858 350 3001 2219
Fragments arranged by size:
3001 2219 858 617 350
BsaAI yAC'GTr
Cuts at: 493 1204 3459 4836 493
Size: 711 2255 1377 2702
Fragments arranged by size:
2702 2255 1377 711
BsaBI GATnn'nnATC
Cuts at: 2805 7039 2805
Size: 4234 2811
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2119 2265 4852 5234
Size: 53 83 186 936 586 146 2587 382
Cuts at: 5234 5869 275
Size: 635 1451
Fragments arranged by size:
2587 1451 936 635 586 382 186 146
83 53
BsaWI w'CCGG_w
Cuts at: 5478 5876 6150 6297 6708 5478
Size: 398 274 147 411 5815
Fragments arranged by size:
5815 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6820
Fragments arranged by size:
6820 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7045
BseYI C'CCAG_C
Cuts at: 6248 6744 6248
Size: 496 6549
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6472
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2078 3237 4495 5256 6284 1540
Size: 275 186 77 1159 1258 761 1028 2301
Fragments arranged by size:
2301 1258 1159 1028 761 275 186 77
BsiHKAI G_wGCw'C
Cuts at: 2094 3251 5106 5191 6262 2094
Size: 1157 1855 85 1071 2877
Fragments arranged by size:
2877 1855 1157 1071 85
BsmI GAATG_Cn'
Cuts at: 4556 4649 4556
Size: 93 6952
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2214 2578 3251 4364 5106 5191
Size: 399 304 120 364 673 1113 742 85
Cuts at: 5191 6262 1391
Size: 1071 2174
Fragments arranged by size:
2174 1113 1071 742 673 399 364 304
120 85
BspEI T'CCGG_A
Cuts at: 6708 6708
Size: 7045
BspHI T'CATG_A
Cuts at: 4934 6664 4934
Size: 1730 5315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2013 4400 844
Size: 1169 2387 3489
Fragments arranged by size:
3489 2387 1169
BsrBI CCG'CTC
Cuts at: 1394 1823 2116 4932 1394
Size: 429 293 2816 3507
Fragments arranged by size:
3507 2816 429 293
BsrDI GCAATG_nn'
Cuts at: 65 1248 2509 3159 3682 4124 5540 5714 65
Size: 1183 1261 650 523 442 1416 174 1396
Fragments arranged by size:
1416 1396 1261 1183 650 523 442 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2077 5689 5876 1700
Size: 110 267 3612 187 2869
Fragments arranged by size:
3612 2869 267 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7045
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7045
BssSI C'ACGA_G
Cuts at: 2111 5105 6117 2111
Size: 2994 1012 3039
Fragments arranged by size:
3039 2994 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7045
BstBI TT'CG_AA
Cuts at: 5849 5849
Size: 7045
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7045
BstXI CCAn_nnnn'nTGG
Cuts at: 3076 3478 3076
Size: 402 6643
BstYI r'GATC_y
Cuts at: 1972 2450 2653 3487 3871 4375 4742 5127 5144
Size: 478 203 834 384 504 367 385 17
Cuts at: 5144 6596 6682 6694 7040 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 834 504 478 385 384 367
346 203 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5554
Fragments arranged by size:
5554 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3055 487
Size: 683 56 1829 4477
Fragments arranged by size:
4477 1829 683 56
BtsI GCAGTG_nn'
Cuts at: 2535 2820 3102 3216 4028 4561 5355 5375 2535
Size: 285 282 114 812 533 794 20 4205
Fragments arranged by size:
4205 812 794 533 285 282 114 20
ClaI AT'CG_AT
Cuts at: 1554 3141 1554
Size: 1587 5458
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7010
DraI TTT'AAA
Cuts at: 3691 4349 3691
Size: 658 6387
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6052 809
Size: 507 4736 1802
Fragments arranged by size:
4736 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2075 2154 2295
Size: 54 1425 50 275 18 245 79 141
Cuts at: 2295 3210 3234 4492 5381 7010 8
Size: 915 24 1258 889 1629 43
Fragments arranged by size:
1629 1425 1258 915 889 275 245 141
79 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2075 3234 4492 1537
Size: 275 263 1159 1258 4090
Fragments arranged by size:
4090 1258 1159 275 263
EarI CTCTTCn'nnn_
Cuts at: 1353 4975 1353
Size: 3622 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5632 6006 6152 387
Size: 1064 138 4043 374 146 1280
Fragments arranged by size:
4043 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2092 2092
Size: 7045
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3273 3896 4133 5108 5705 6492 6760
Size: 393 1641 623 237 975 597 787 268
Cuts at: 6760 1239
Size: 1524
Fragments arranged by size:
1641 1524 975 787 623 597 393 268
237
EcoRI G'AATT_C
Cuts at: 3155 4049 4354 4732 3155
Size: 894 305 378 5468
Fragments arranged by size:
5468 894 378 305
EcoRV GAT'ATC
Cuts at: 3589 3889 3589
Size: 300 6745
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2203 3367 4286 4322 2203
Size: 1164 919 36 4926
Fragments arranged by size:
4926 1164 919 36
FspI TGC'GCA
Cuts at: 5551 5551
Size: 7045
HaeII r_GCGC'y
Cuts at: 1678 2015 2122 3758 6192 1678
Size: 337 107 1636 2434 2531
Fragments arranged by size:
2531 2434 1636 337 107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2337 2369 2874 2906 3911
Size: 32 135 32 765 32 505 32 1005
Cuts at: 3911 3943 5686 5718 5760 5792 1373
Size: 32 1743 32 42 32 2626
Fragments arranged by size:
2626 1743 1005 765 505 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3525 4389 4635 669
Size: 988 281 1587 864 246 3079
Fragments arranged by size:
3079 1587 988 864 281 246
HindIII A'AGCT_T
Cuts at: 748 3323 748
Size: 2575 4470
HpaI GTT'AAC
Cuts at: 4635 4635
Size: 7045
KasI G'GCGC_C
Cuts at: 2118 2118
Size: 7045
KpnI G_GTAC'C
Cuts at: 4370 4370
Size: 7045
MfeI C'AATT_G
Cuts at: 4644 4644
Size: 7045
MluI A'CGCG_T
Cuts at: 6921 6921
Size: 7045
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2007 2323 2398 2572 3694 3997 6159 6343
Size: 702 316 75 174 1122 303 2162 184
Cuts at: 6343 1305
Size: 2007
Fragments arranged by size:
2162 2007 1122 702 316 303 184 174
75
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2156 2297 3212 10
Size: 54 1425 343 324 141 915 3843
Fragments arranged by size:
3843 1425 915 343 324 141 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3074 5003 5362 5521 518
Size: 648 1908 1929 359 159 2042
Fragments arranged by size:
2042 1929 1908 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2055 2681 3359 3397 4461 5138 6286 6531
Size: 449 626 678 38 1064 677 1148 245
Cuts at: 6531 1606
Size: 2120
Fragments arranged by size:
2120 1148 1064 678 677 626 449 245
38
NarI GG'CG_CC
Cuts at: 2119 2119
Size: 7045
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5554
Fragments arranged by size:
5554 939 552
NdeI CA'TA_TG
Cuts at: 387 2317 2947 387
Size: 1930 630 4485
Fragments arranged by size:
4485 1930 630
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7045
NotI GC'GGCC_GC
Cuts at: 4492 4492
Size: 7045
NspI r_CATG'y
Cuts at: 1132 1229 4403 5948 1132
Size: 97 3174 1545 2229
Fragments arranged by size:
3174 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3316 1348
Size: 1968 5077
PciI A'CATG_T
Cuts at: 5944 5944
Size: 7045
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2698 1108
Size: 1590 5455
PfoI T'CCnGG_A
Cuts at: 3240 4005 4314 3240
Size: 765 309 5971
Fragments arranged by size:
5971 765 309
PmeI GTTT'AAAC
Cuts at: 4349 4349
Size: 7045
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2472 2504 5114 5146 6627 6659 2472
Size: 32 2610 32 1481 32 2858
Fragments arranged by size:
2858 2610 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3530 1613
Size: 1917 5128
PsiI TTA'TAA
Cuts at: 4615 4615
Size: 7045
PstI C_TGCA'G
Cuts at: 830 2060 2127 2168 2335 2542 4397 830
Size: 1230 67 41 167 207 1855 3478
Fragments arranged by size:
3478 1855 1230 207 167 67 41
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7045
PvuII CAG'CTG
Cuts at: 2055 2681 3359 3397 2055
Size: 626 678 38 5703
Fragments arranged by size:
5703 678 626 38
SacI G_AGCT'C
Cuts at: 2094 2094
Size: 7045
SalI G'TCGA_C
Cuts at: 1936 4387 1936
Size: 2451 4594
SbfI CC_TGCA'GG
Cuts at: 2127 4397 2127
Size: 2270 4775
ScaI AGT'ACT
Cuts at: 2284 5293 7005 2284
Size: 3009 1712 2324
Fragments arranged by size:
3009 2324 1712
SfcI C'TryA_G
Cuts at: 826 1045 2056 2083 2123 2164 2331 2538 2907
Size: 219 1011 27 40 41 167 207 369
Cuts at: 2907 4190 4393 5528 6209 6400 826
Size: 1283 203 1135 681 191 1471
Fragments arranged by size:
1471 1283 1135 1011 681 369 219 207
203 191 167 41 40 27
SfoI GGC'GCC
Cuts at: 2120 2120
Size: 7045
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7045
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7045
SmaI CCC'GGG
Cuts at: 1872 4372 1872
Size: 2500 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3630 4209 5149 6050 6312 820
Size: 197 926 1687 579 940 901 262 1553
Fragments arranged by size:
1687 1553 940 926 901 579 262 197
SnaBI TAC'GTA
Cuts at: 493 4836 493
Size: 4343 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7045
SphI G_CATG'C
Cuts at: 1132 4403 1132
Size: 3271 3774
SspI AAT'ATT
Cuts at: 5 52 2794 4969 5
Size: 47 2742 2175 2081
Fragments arranged by size:
2742 2175 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7045
StyI C'CwwG_G
Cuts at: 513 1065 2004 2999 3565 3897 4484 513
Size: 552 939 995 566 332 587 3074
Fragments arranged by size:
3074 995 939 587 566 552 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3337 3439 5072 5089 5242 5427 5872
Size: 1255 1757 102 1633 17 153 185 445
Cuts at: 5872 325
Size: 1498
Fragments arranged by size:
1757 1633 1498 1255 445 185 153 102
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2049 2282 5291
Size: 274 80 33 51 1443 72 233 3009
Cuts at: 5291 7003 96
Size: 1712 138
Fragments arranged by size:
3009 1712 1443 274 233 138 80 72
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2622 2654 7005 7037 2622
Size: 32 4351 32 2630
Fragments arranged by size:
4351 2630 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7045
XbaI T'CTAG_A
Cuts at: 4381 4381
Size: 7045
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3076 3248 3313 3076
Size: 172 65 6808
Fragments arranged by size:
6808 172 65
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7045
XmaI C'CCGG_G
Cuts at: 1870 4370 1870
Size: 2500 4545
XmnI GAAnn'nnTTC
Cuts at: 2484 5174 2484
Size: 2690 4355
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2266 4853 276
Size: 53 83 186 936 732 2587 2468
Fragments arranged by size:
2587 2468 936 732 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI
SwaI