Restriction map for
pFN21AE0372
(Circular) (Six-base) MAPSORT of: pFN21AE0372.seq Check: 201 from: 1 to: 6400
pFN21AE0372 6400 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3396 4210 278
Size: 53 83 186 936 1860 814 2468
Fragments arranged by size:
2468 1860 936 814 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2653 3186 3220 3743 1937
Size: 716 533 34 523 4594
Fragments arranged by size:
4594 716 533 523 34
Acc65I G'GTAC_C
Cuts at: 3721 3721
Size: 6400
AclI AA'CG_TT
Cuts at: 1563 4527 4900 1563
Size: 2964 373 3063
Fragments arranged by size:
3063 2964 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2094 3192 4463 5847 6115 1632
Size: 462 1098 1271 1384 268 1917
Fragments arranged by size:
1917 1384 1271 1098 462 268
AflII C'TTAA_G
Cuts at: 820 1017 3397 820
Size: 197 2380 3823
Fragments arranged by size:
3823 2380 197
AflIII A'CryG_T
Cuts at: 1203 2477 3638 5299 6276 1203
Size: 1274 1161 1661 977 1327
Fragments arranged by size:
1661 1327 1274 1161 977
AgeI A'CCGG_T
Cuts at: 5231 5231
Size: 6400
AhdI GACnn_n'nnGTC
Cuts at: 5129 5129
Size: 6400
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6400
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6400
AlwNI CAG_nnn'CTG
Cuts at: 2132 4143 5715 2132
Size: 2011 1572 2817
Fragments arranged by size:
2817 2011 1572
ApaLI G'TGCA_C
Cuts at: 4457 5613 4457
Size: 1156 5244
ApoI r'AATT_y
Cuts at: 1493 2405 2761 3194 3709 3935 4087 6196 1493
Size: 912 356 433 515 226 152 2109 1697
Fragments arranged by size:
2109 1697 912 515 433 356 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2855 4954 160
Size: 872 1823 2099 1606
Fragments arranged by size:
2099 1823 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6400
AvaI C'yCGr_G
Cuts at: 1870 1943 3725 1870
Size: 73 1782 4545
Fragments arranged by size:
4545 1782 73
BamHI G'GATC_C
Cuts at: 3730 4097 3730
Size: 367 6033
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3721 5176 618
Size: 325 217 627 1934 1455 1842
Fragments arranged by size:
1934 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2084 2474 3719 1391
Size: 693 390 1245 4072
Fragments arranged by size:
4072 1245 693 390
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2008 2802 928
Size: 971 109 794 4526
Fragments arranged by size:
4526 971 794 109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4591 4625 1903
Size: 34 2654 34 3678
Fragments arranged by size:
3678 2654 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4292 5502 4292
Size: 1210 5190
BclI T'GATC_A
Cuts at: 1548 2518 2859 1548
Size: 970 341 5089
Fragments arranged by size:
5089 970 341
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5011 136
Size: 107 122 71 631 605 3339 1525
Fragments arranged by size:
3339 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2557 6395 2557
Size: 3838 2562
BlpI GC'TnA_GC
Cuts at: 3817 3817
Size: 6400
Bme1580I G_kGCm'C
Cuts at: 1790 2305 4461 5617 1790
Size: 515 2156 1156 2573
Fragments arranged by size:
2573 2156 1156 515
BmgBI CAC'GTC
Cuts at: 1318 3599 1318
Size: 2281 4119
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4131 5089 442
Size: 416 53 83 399 24 2714 958 1753
Fragments arranged by size:
2714 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6400
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2178 3162 2178
Size: 984 5416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2403 2982 3135 5060 1239
Size: 1164 579 153 1925 2579
Fragments arranged by size:
2579 1925 1164 579 153
Bpu10I CC'TnA_GC
Cuts at: 3137 3137
Size: 6400
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2660 4525 5390 5688 2660
Size: 1865 865 298 3372
Fragments arranged by size:
3372 1865 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2380 5063 882
Size: 617 881 2683 2219
Fragments arranged by size:
2683 2219 881 617
BsaAI yAC'GTr
Cuts at: 493 1204 2480 4191 493
Size: 711 1276 1711 2702
Fragments arranged by size:
2702 1711 1276 711
BsaBI GATnn'nnATC
Cuts at: 6394 6394
Size: 6400
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3393 4207 4589 5224
Size: 53 83 186 936 1860 814 382 635
Cuts at: 5224 275
Size: 1451
Fragments arranged by size:
1860 1451 936 814 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4833 5231 5505 5652 6063 4833
Size: 398 274 147 411 5170
Fragments arranged by size:
5170 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3111 3141 3225 3255 1178
Size: 30 165 30 1708 30 84 30 4323
Fragments arranged by size:
4323 1708 165 84 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2510 2685 3138 1186
Size: 1324 175 453 4448
Fragments arranged by size:
4448 1324 453 175
BseYI C'CCAG_C
Cuts at: 5603 6099 5603
Size: 496 5904
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5827
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3850 4611 5639 1540
Size: 275 186 1849 761 1028 2301
Fragments arranged by size:
2301 1849 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2084 4461 4546 5617 2084
Size: 2377 85 1071 2867
Fragments arranged by size:
2867 2377 1071 85
BsmI GAATG_Cn'
Cuts at: 3182 3911 4004 3182
Size: 729 93 5578
Fragments arranged by size:
5578 729 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2305 2474 3719 4461 4546 5617
Size: 399 294 221 169 1245 742 85 1071
Cuts at: 5617 1391
Size: 2174
Fragments arranged by size:
2174 1245 1071 742 399 294 221 169
85
BspEI T'CCGG_A
Cuts at: 6063 6063
Size: 6400
BspHI T'CATG_A
Cuts at: 4289 6019 4289
Size: 1730 4670
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3755 844
Size: 2911 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4287 1394
Size: 429 2464 3507
Fragments arranged by size:
3507 2464 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2103 3087 4895 5069 65
Size: 1183 855 984 1808 174 1396
Fragments arranged by size:
1808 1396 1183 984 855 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5044 5231 1700
Size: 110 3234 187 2869
Fragments arranged by size:
3234 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6400
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6400
BssSI C'ACGA_G
Cuts at: 4460 5472 4460
Size: 1012 5388
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2894 794
Size: 2100 4300
BstBI TT'CG_AA
Cuts at: 5204 5204
Size: 6400
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6400
BstYI r'GATC_y
Cuts at: 1972 2557 3730 4097 4482 4499 5951 6037 6049
Size: 585 1173 367 385 17 1452 86 12
Cuts at: 6049 6395 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1173 585 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3221 3221
Size: 6400
Bsu36I CC'TnA_GG
Cuts at: 3115 3115
Size: 6400
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4909
Fragments arranged by size:
4909 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5661
Fragments arranged by size:
5661 683 56
BtsI GCAGTG_nn'
Cuts at: 3299 3916 4710 4730 3299
Size: 617 794 20 4969
Fragments arranged by size:
4969 794 617 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6400
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6365
DraI TTT'AAA
Cuts at: 3704 3704
Size: 6400
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3392 5407 809
Size: 507 2076 2015 1802
Fragments arranged by size:
2076 2015 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2749 3280 3529
Size: 54 1425 50 275 18 919 531 249
Cuts at: 3529 3847 4736 6365 8
Size: 318 889 1629 43
Fragments arranged by size:
1629 1425 919 889 531 318 275 249
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3847 1537
Size: 275 2035 4090
Fragments arranged by size:
4090 2035 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2979 4330 1353
Size: 1626 1351 3423
Fragments arranged by size:
3423 1626 1351
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4987 5361 5507 387
Size: 1064 138 3398 374 146 1280
Fragments arranged by size:
3398 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2082 2082
Size: 6400
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2403 2982 3135 3192 4463 5060
Size: 393 462 309 579 153 57 1271 597
Cuts at: 5060 5847 6115 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1271 787 597 579 462 393 309
268 153 57
EcoRI G'AATT_C
Cuts at: 3709 4087 3709
Size: 378 6022
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2383 2922 2383
Size: 539 5861
FspI TGC'GCA
Cuts at: 4906 4906
Size: 6400
HaeII r_GCGC'y
Cuts at: 1678 5547 1678
Size: 3869 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2867 2899 3588 3620 5041
Size: 32 135 32 1295 32 689 32 1421
Cuts at: 5041 5073 5115 5147 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1421 1295 689 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3646 3744 3990 669
Size: 988 281 1708 98 246 3079
Fragments arranged by size:
3079 1708 988 281 246 98
HindIII A'AGCT_T
Cuts at: 748 3054 748
Size: 2306 4094
HpaI GTT'AAC
Cuts at: 3646 3990 3646
Size: 344 6056
KpnI G_GTAC'C
Cuts at: 3725 3725
Size: 6400
MfeI C'AATT_G
Cuts at: 2292 3300 3999 2292
Size: 1008 699 4693
Fragments arranged by size:
4693 1008 699
MluI A'CGCG_T
Cuts at: 6276 6276
Size: 6400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2293 3547 5514 5698 1305
Size: 988 1254 1967 184 2007
Fragments arranged by size:
2007 1967 1254 988 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2751 3282 10
Size: 54 1425 343 919 531 3128
Fragments arranged by size:
3128 1425 919 531 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2369 2456 2952 2993 3443 4358 4717
Size: 648 1203 87 496 41 450 915 359
Cuts at: 4717 4876 518
Size: 159 2042
Fragments arranged by size:
2042 1203 915 648 496 450 359 159
87 41
MspA1I CmG'CkG
Cuts at: 1606 2381 3816 4493 5641 5886 1606
Size: 775 1435 677 1148 245 2120
Fragments arranged by size:
2120 1435 1148 775 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4909
Fragments arranged by size:
4909 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6400
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6400
NotI GC'GGCC_GC
Cuts at: 3847 3847
Size: 6400
NspI r_CATG'y
Cuts at: 1132 1229 3642 3758 5303 1132
Size: 97 2413 116 1545 2229
Fragments arranged by size:
2413 2229 1545 116 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6400
PciI A'CATG_T
Cuts at: 3638 5299 3638
Size: 1661 4739
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3505 1108
Size: 2397 4003
PfoI T'CCnGG_A
Cuts at: 2177 2177
Size: 6400
PmeI GTTT'AAAC
Cuts at: 3704 3704
Size: 6400
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4469 4501 5982 6014 4469
Size: 32 1481 32 4855
Fragments arranged by size:
4855 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6400
PsiI TTA'TAA
Cuts at: 3062 3213 3970 3062
Size: 151 757 5492
Fragments arranged by size:
5492 757 151
PstI C_TGCA'G
Cuts at: 830 2890 3752 830
Size: 2060 862 3478
Fragments arranged by size:
3478 2060 862
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6400
PvuII CAG'CTG
Cuts at: 2381 2381
Size: 6400
SacI G_AGCT'C
Cuts at: 2084 2084
Size: 6400
SalI G'TCGA_C
Cuts at: 1936 3742 1936
Size: 1806 4594
SbfI CC_TGCA'GG
Cuts at: 3752 3752
Size: 6400
ScaI AGT'ACT
Cuts at: 4648 6360 4648
Size: 1712 4688
SfcI C'TryA_G
Cuts at: 826 1045 2650 2886 3748 4883 5564 5755 826
Size: 219 1605 236 862 1135 681 191 1471
Fragments arranged by size:
1605 1471 1135 862 681 236 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6400
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6400
SmaI CCC'GGG
Cuts at: 1872 3727 1872
Size: 1855 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2639 3397 4504 5405 5667 820
Size: 197 926 696 758 1107 901 262 1553
Fragments arranged by size:
1553 1107 926 901 758 696 262 197
SnaBI TAC'GTA
Cuts at: 493 4191 493
Size: 3698 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6400
SphI G_CATG'C
Cuts at: 1132 3758 1132
Size: 2626 3774
SspI AAT'ATT
Cuts at: 5 52 2680 2984 4324 5
Size: 47 2628 304 1340 2081
Fragments arranged by size:
2628 2081 1340 304 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6400
StyI C'CwwG_G
Cuts at: 513 1065 2004 2073 2165 3839 513
Size: 552 939 69 92 1674 3074
Fragments arranged by size:
3074 1674 939 552 92 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2590 4427 4444 4597 4782 5227 325
Size: 1255 1010 1837 17 153 185 445 1498
Fragments arranged by size:
1837 1498 1255 1010 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4646 6358 96
Size: 274 80 33 51 1443 2669 1712 138
Fragments arranged by size:
2669 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6360 6392 6360
Size: 32 6368
Tth111I GACn'n_nGTC
Cuts at: 1316 3308 1316
Size: 1992 4408
XbaI T'CTAG_A
Cuts at: 3736 3736
Size: 6400
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2185 2276 3502 2185
Size: 91 1226 5083
Fragments arranged by size:
5083 1226 91
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6400
XmaI C'CCGG_G
Cuts at: 1870 3725 1870
Size: 1855 4545
XmnI GAAnn'nnTTC
Cuts at: 4529 4529
Size: 6400
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3394 4208 276
Size: 53 83 186 936 1860 814 2468
Fragments arranged by size:
2468 1860 936 814 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstXI DraIII EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI