Restriction map for
pFN21AE0371
(Circular) (Six-base) MAPSORT of: pFN21AE0371.seq Check: 5056 from: 1 to: 6391
pFN21AE0371 6391 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4201 278
Size: 53 83 186 936 2665 2468
Fragments arranged by size:
2665 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3678 3734 1937
Size: 1741 56 4594
Fragments arranged by size:
4594 1741 56
Acc65I G'GTAC_C
Cuts at: 2275 3712 2275
Size: 1437 4954
AclI AA'CG_TT
Cuts at: 1563 4518 4891 1563
Size: 2955 373 3063
Fragments arranged by size:
3063 2955 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3402 4454 5838 6106 1632
Size: 1770 1052 1384 268 1917
Fragments arranged by size:
1917 1770 1384 1052 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6194
AflIII A'CryG_T
Cuts at: 1203 2187 3258 3369 5290 6267 1203
Size: 984 1071 111 1921 977 1327
Fragments arranged by size:
1921 1327 1071 984 977 111
AgeI A'CCGG_T
Cuts at: 5222 5222
Size: 6391
AhdI GACnn_n'nnGTC
Cuts at: 5120 5120
Size: 6391
AleI CACnn'nnGTG
Cuts at: 1166 2186 1166
Size: 1020 5371
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6391
AlwNI CAG_nnn'CTG
Cuts at: 2977 3196 4134 5706 2977
Size: 219 938 1572 3662
Fragments arranged by size:
3662 1572 938 219
ApaI G_GGCC'C
Cuts at: 2030 2063 3550 2030
Size: 33 1487 4871
Fragments arranged by size:
4871 1487 33
ApaLI G'TGCA_C
Cuts at: 2582 4448 5604 2582
Size: 1866 1156 3369
Fragments arranged by size:
3369 1866 1156
ApoI r'AATT_y
Cuts at: 1493 3700 3926 4078 6187 1493
Size: 2207 226 152 2109 1697
Fragments arranged by size:
2207 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4945 160
Size: 872 3913 1606
Fragments arranged by size:
3913 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
AvaI C'yCGr_G
Cuts at: 1870 1943 3293 3395 3716 1870
Size: 73 1350 102 321 4545
Fragments arranged by size:
4545 1350 321 102 73
BamHI G'GATC_C
Cuts at: 2232 3721 4088 2232
Size: 1489 367 4535
Fragments arranged by size:
4535 1489 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2047 2275 3125 3712 5167
Size: 325 217 627 260 228 850 587 1455
Cuts at: 5167 618
Size: 1842
Fragments arranged by size:
1842 1455 850 627 587 325 260 228
217
BanII G_rGCy'C
Cuts at: 1391 2030 2042 2063 3210 3330 3550 3710 1391
Size: 639 12 21 1147 120 220 160 4072
Fragments arranged by size:
4072 1147 639 220 160 120 21 12
BbeI G_GCGC'C
Cuts at: 2051 2051
Size: 6391
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2353 2914 928
Size: 971 454 561 4405
Fragments arranged by size:
4405 971 561 454
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2197 2231 4582 4616 1903
Size: 34 260 34 2351 34 3678
Fragments arranged by size:
3678 2351 260 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4283 5493 4283
Size: 1210 5181
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6391
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3039 3545 5002
Size: 107 122 71 631 605 1367 506 1457
Cuts at: 5002 136
Size: 1525
Fragments arranged by size:
1525 1457 1367 631 605 506 122 107
71
BglII A'GATC_T
Cuts at: 2706 6386 2706
Size: 3680 2711
BlpI GC'TnA_GC
Cuts at: 3300 3808 3300
Size: 508 5883
Bme1580I G_kGCm'C
Cuts at: 1790 2030 2063 2586 3185 3550 4452 5608 1790
Size: 240 33 523 599 365 902 1156 2573
Fragments arranged by size:
2573 1156 902 599 523 365 240 33
BmgBI CAC'GTC
Cuts at: 1318 2188 2494 3109 1318
Size: 870 306 615 4600
Fragments arranged by size:
4600 870 615 306
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4122 5080 442
Size: 416 53 83 399 24 2705 958 1753
Fragments arranged by size:
2705 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2365 3692 5051 1239
Size: 1126 1327 1359 2579
Fragments arranged by size:
2579 1359 1327 1126
Bpu10I CC'TnA_GC
Cuts at: 3035 3035
Size: 6391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4516 5381 5679 4516
Size: 865 298 5228
Fragments arranged by size:
5228 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2476 2525 2858 5054 882
Size: 617 977 49 333 2196 2219
Fragments arranged by size:
2219 2196 977 617 333 49
BsaAI yAC'GTr
Cuts at: 493 1204 3682 4182 493
Size: 711 2478 500 2702
Fragments arranged by size:
2702 2478 711 500
BsaBI GATnn'nnATC
Cuts at: 6385 6385
Size: 6391
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2048 3472 4198 4580
Size: 53 83 186 936 515 1424 726 382
Cuts at: 4580 5215 275
Size: 635 1451
Fragments arranged by size:
1451 1424 936 726 635 515 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4824 5222 5496 5643 6054 4824
Size: 398 274 147 411 5161
Fragments arranged by size:
5161 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3578 3608 1178
Size: 30 165 30 2175 30 3961
Fragments arranged by size:
3961 2175 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2117 2237 2685 1186
Size: 841 90 120 448 4892
Fragments arranged by size:
4892 841 448 120 90
BseYI C'CCAG_C
Cuts at: 3240 3410 5594 6090 3240
Size: 170 2184 496 3541
Fragments arranged by size:
3541 2184 496 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2119 2326 2402 1163
Size: 573 383 207 76 5152
Fragments arranged by size:
5152 573 383 207 76
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2565 2667 2697 3841 4602 5630
Size: 275 186 564 102 30 1144 761 1028
Cuts at: 5630 1540
Size: 2301
Fragments arranged by size:
2301 1144 1028 761 564 275 186 102
30
BsiHKAI G_wGCw'C
Cuts at: 2586 4452 4537 5608 2586
Size: 1866 85 1071 3369
Fragments arranged by size:
3369 1866 1071 85
BsmI GAATG_Cn'
Cuts at: 3902 3995 3902
Size: 93 6298
BsmBI CGTCTCn'nnnn_
Cuts at: 2639 3451 3463 2639
Size: 812 12 5567
Fragments arranged by size:
5567 812 12
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2042 2063 2586 3185 3210 3330
Size: 399 240 12 21 523 599 25 120
Cuts at: 3330 3550 3710 4452 4537 5608 1391
Size: 220 160 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 599 523 399 240 220
160 120 85 25 21 12
BspEI T'CCGG_A
Cuts at: 6054 6054
Size: 6391
BspHI T'CATG_A
Cuts at: 3231 4280 6010 3231
Size: 1049 1730 3612
Fragments arranged by size:
3612 1730 1049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3204 3746 844
Size: 2360 542 3489
Fragments arranged by size:
3489 2360 542
BsrBI CCG'CTC
Cuts at: 1394 1823 2949 3039 4278 1394
Size: 429 1126 90 1239 3507
Fragments arranged by size:
3507 1239 1126 429 90
BsrDI GCAATG_nn'
Cuts at: 65 1248 3520 4886 5060 65
Size: 1183 2272 1366 174 1396
Fragments arranged by size:
2272 1396 1366 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2369 5035 5222 1700
Size: 110 559 2666 187 2869
Fragments arranged by size:
2869 2666 559 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6391
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6391
BssSI C'ACGA_G
Cuts at: 4451 5463 4451
Size: 1012 5379
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6391
BstBI TT'CG_AA
Cuts at: 5195 5195
Size: 6391
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6391
BstXI CCAn_nnnn'nTGG
Cuts at: 2545 2557 3670 2545
Size: 12 1113 5266
Fragments arranged by size:
5266 1113 12
BstYI r'GATC_y
Cuts at: 1972 2232 2412 2503 2706 3721 4088 4473 4490
Size: 260 180 91 203 1015 367 385 17
Cuts at: 4490 5942 6028 6040 6386 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1015 385 367 346 260 203
180 91 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2565 3072 513
Size: 552 1500 507 3832
Fragments arranged by size:
3832 1500 552 507
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2222 487
Size: 683 56 996 4656
Fragments arranged by size:
4656 996 683 56
BtsI GCAGTG_nn'
Cuts at: 2202 3224 3441 3907 4701 4721 2202
Size: 1022 217 466 794 20 3872
Fragments arranged by size:
3872 1022 794 466 217 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6391
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6356
DraI TTT'AAA
Cuts at: 3695 3695
Size: 6391
DraIII CAC_nnn'GTG
Cuts at: 3658 3658
Size: 6391
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2663 5398 809
Size: 507 1347 2735 1802
Fragments arranged by size:
2735 1802 1347 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2086 2169 2547
Size: 54 1425 50 275 18 256 83 378
Cuts at: 2547 2562 2694 2824 3438 3838 4727 6356 8
Size: 15 132 130 614 400 889 1629 43
Fragments arranged by size:
1629 1425 889 614 400 378 275 256
132 130 83 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2562 2694 3838 1537
Size: 275 750 132 1144 4090
Fragments arranged by size:
4090 1144 750 275 132
EarI CTCTTCn'nnn_
Cuts at: 1353 2243 2765 2861 2906 4321 1353
Size: 890 522 96 45 1415 3423
Fragments arranged by size:
3423 1415 890 522 96 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2166 3367 4978 5352 5498 387
Size: 1064 138 577 1201 1611 374 146 1280
Fragments arranged by size:
1611 1280 1201 1064 577 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2365 3402 3692 4454 5051 5838 6106
Size: 393 733 1037 290 762 597 787 268
Cuts at: 6106 1239
Size: 1524
Fragments arranged by size:
1524 1037 787 762 733 597 393 290
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2429 3671 2429
Size: 1242 5149
EcoO109I rG'GnC_Cy
Cuts at: 2026 2027 2060 2974 3193 3546 2026
Size: 1 33 914 219 353 4871
Fragments arranged by size:
4871 914 353 219 33 1
EcoRI G'AATT_C
Cuts at: 3700 4078 3700
Size: 378 6013
FspI TGC'GCA
Cuts at: 4897 4897
Size: 6391
HaeII r_GCGC'y
Cuts at: 1678 2051 5538 1678
Size: 373 3487 2531
Fragments arranged by size:
3487 2531 373
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2384 2416 5032 5064 5106
Size: 32 135 32 812 32 2616 32 42
Cuts at: 5106 5138 1373
Size: 32 2626
Fragments arranged by size:
2626 2616 812 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3616 3735 3981 669
Size: 988 281 1678 119 246 3079
Fragments arranged by size:
3079 1678 988 281 246 119
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6391
HpaI GTT'AAC
Cuts at: 3981 3981
Size: 6391
KasI G'GCGC_C
Cuts at: 2047 2047
Size: 6391
KpnI G_GTAC'C
Cuts at: 2279 3716 2279
Size: 1437 4954
MfeI C'AATT_G
Cuts at: 3990 3990
Size: 6391
MluI A'CGCG_T
Cuts at: 6267 6267
Size: 6391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2647 5505 5689 1305
Size: 1342 2858 184 2007
Fragments arranged by size:
2858 2007 1342 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2549 2826 3440 10
Size: 54 1425 343 717 277 614 2961
Fragments arranged by size:
2961 1425 717 614 343 277 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 2207 2555 4349 4708 4867 518
Size: 648 1020 21 348 1794 359 159 2042
Fragments arranged by size:
2042 1794 1020 648 359 348 159 21
MspA1I CmG'CkG
Cuts at: 1606 2148 3427 3807 4484 5632 5877 1606
Size: 542 1279 380 677 1148 245 2120
Fragments arranged by size:
2120 1279 1148 677 542 380 245
NarI GG'CG_CC
Cuts at: 2048 2048
Size: 6391
NcoI C'CATG_G
Cuts at: 513 1065 3072 513
Size: 552 2007 3832
Fragments arranged by size:
3832 2007 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6391
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6391
NotI GC'GGCC_GC
Cuts at: 3838 3838
Size: 6391
NspI r_CATG'y
Cuts at: 1132 1229 2654 3262 3652 3749 5294 1132
Size: 97 1425 608 390 97 1545 2229
Fragments arranged by size:
2229 1545 1425 608 390 97 97
PasI CC'CwG_GG
Cuts at: 1348 2023 2064 3133 1348
Size: 675 41 1069 4606
Fragments arranged by size:
4606 1069 675 41
PciI A'CATG_T
Cuts at: 3258 5290 3258
Size: 2032 4359
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6391
PfoI T'CCnGG_A
Cuts at: 2859 2859
Size: 6391
PmeI GTTT'AAAC
Cuts at: 3695 3695
Size: 6391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4460 4492 5973 6005 4460
Size: 32 1481 32 4846
Fragments arranged by size:
4846 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3193 3193
Size: 6391
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6391
PsiI TTA'TAA
Cuts at: 3961 3961
Size: 6391
PspOMI G'GGCC_C
Cuts at: 2026 2059 3546 2026
Size: 33 1487 4871
Fragments arranged by size:
4871 1487 33
PstI C_TGCA'G
Cuts at: 830 2690 3743 830
Size: 1860 1053 3478
Fragments arranged by size:
3478 1860 1053
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6391
SalI G'TCGA_C
Cuts at: 1936 3733 1936
Size: 1797 4594
SapI GCTCTTCn'nnn_
Cuts at: 2906 2906
Size: 6391
SbfI CC_TGCA'GG
Cuts at: 3743 3743
Size: 6391
ScaI AGT'ACT
Cuts at: 4639 6351 4639
Size: 1712 4679
SexAI A'CCwGG_T
Cuts at: 2510 2510
Size: 6391
SfcI C'TryA_G
Cuts at: 826 1045 2599 2686 2989 3739 4874 5555 5746
Size: 219 1554 87 303 750 1135 681 191
Cuts at: 5746 826
Size: 1471
Fragments arranged by size:
1554 1471 1135 750 681 303 219 191
87
SfoI GGC'GCC
Cuts at: 2049 2049
Size: 6391
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6391
SmaI CCC'GGG
Cuts at: 1872 3295 3397 3718 1872
Size: 1423 102 321 4545
Fragments arranged by size:
4545 1423 321 102
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4495 5396 5658 820
Size: 197 926 2552 901 262 1553
Fragments arranged by size:
2552 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4182 493
Size: 3689 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6391
SphI G_CATG'C
Cuts at: 1132 2654 3652 3749 1132
Size: 1522 998 97 3774
Fragments arranged by size:
3774 1522 998 97
SspI AAT'ATT
Cuts at: 5 52 4315 5
Size: 47 4263 2081
Fragments arranged by size:
4263 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6391
StyI C'CwwG_G
Cuts at: 513 1065 2031 3024 3072 3321 3830 513
Size: 552 966 993 48 249 509 3074
Fragments arranged by size:
3074 993 966 552 509 249 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3113 3233 3403 4418 4435 4588 4773
Size: 1255 1533 120 170 1015 17 153 185
Cuts at: 4773 5218 325
Size: 445 1498
Fragments arranged by size:
1533 1498 1255 1015 445 185 170 153
120 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2751 4637 6349
Size: 274 80 33 51 1443 774 1886 1712
Cuts at: 6349 96
Size: 138
Fragments arranged by size:
1886 1712 1443 774 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2068 2100 3359 3391 3644 3676 6351 6383 2068
Size: 32 1259 32 253 32 2675 32 2076
Fragments arranged by size:
2675 2076 1259 253 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6391
XbaI T'CTAG_A
Cuts at: 3727 3727
Size: 6391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2974 3100 3169 3397 2974
Size: 126 69 228 5968
Fragments arranged by size:
5968 228 126 69
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6391
XmaI C'CCGG_G
Cuts at: 1870 3293 3395 3716 1870
Size: 1423 102 321 4545
Fragments arranged by size:
4545 1423 321 102
XmnI GAAnn'nnTTC
Cuts at: 4520 4520
Size: 6391
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4199 276
Size: 53 83 186 936 2665 2468
Fragments arranged by size:
2665 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI PvuII RsrII SacI SacII SanDI SfiI SrfI
SwaI