Restriction map for
pFN21AE0369
(Circular) (Six-base) MAPSORT of: pFN21AE0369.seq Check: 4037 from: 1 to: 6364
pFN21AE0369 6364 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4174 278
Size: 53 83 186 936 2638 2468
Fragments arranged by size:
2638 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3707 1937
Size: 1770 4594
Acc65I G'GTAC_C
Cuts at: 3685 3685
Size: 6364
AclI AA'CG_TT
Cuts at: 1563 4491 4864 1563
Size: 2928 373 3063
Fragments arranged by size:
3063 2928 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2705 3233 3414 4427 5811 6079 1632
Size: 1073 528 181 1013 1384 268 1917
Fragments arranged by size:
1917 1384 1073 1013 528 268 181
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6167
AflIII A'CryG_T
Cuts at: 1203 2858 5263 6240 1203
Size: 1655 2405 977 1327
Fragments arranged by size:
2405 1655 1327 977
AgeI A'CCGG_T
Cuts at: 5195 5195
Size: 6364
AhdI GACnn_n'nnGTC
Cuts at: 5093 5093
Size: 6364
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2108 1184
Size: 924 5440
AlwNI CAG_nnn'CTG
Cuts at: 2158 2329 3539 4107 5679 2158
Size: 171 1210 568 1572 2843
Fragments arranged by size:
2843 1572 1210 568 171
ApaI G_GGCC'C
Cuts at: 2023 2023
Size: 6364
ApaLI G'TGCA_C
Cuts at: 4421 5577 4421
Size: 1156 5208
ApoI r'AATT_y
Cuts at: 1493 3242 3491 3673 3899 4051 6160 1493
Size: 1749 249 182 226 152 2109 1697
Fragments arranged by size:
2109 1749 1697 249 226 182 152
AseI AT'TA_AT
Cuts at: 160 1032 4918 160
Size: 872 3886 1606
Fragments arranged by size:
3886 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
AvaI C'yCGr_G
Cuts at: 1870 1943 2123 2359 3689 1870
Size: 73 180 236 1330 4545
Fragments arranged by size:
4545 1330 236 180 73
BamHI G'GATC_C
Cuts at: 3694 4061 3694
Size: 367 5997
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3685 5140 618
Size: 325 217 627 1898 1455 1842
Fragments arranged by size:
1898 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2023 2148 3683 1391
Size: 632 125 1535 4072
Fragments arranged by size:
4072 1535 632 125
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5393
BbvCI CC'TCA_GC
Cuts at: 2381 2460 2381
Size: 79 6285
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4555 4589 1903
Size: 34 2618 34 3678
Fragments arranged by size:
3678 2618 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4256 5466 4256
Size: 1210 5154
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6364
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4975 136
Size: 107 122 71 631 605 3303 1525
Fragments arranged by size:
3303 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6359 6359
Size: 6364
BlpI GC'TnA_GC
Cuts at: 3781 3781
Size: 6364
Bme1580I G_kGCm'C
Cuts at: 1790 2023 2238 2353 2858 4425 5581 1790
Size: 233 215 115 505 1567 1156 2573
Fragments arranged by size:
2573 1567 1156 505 233 215 115
BmgBI CAC'GTC
Cuts at: 1318 2272 1318
Size: 954 5410
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2290 2652 4095
Size: 416 53 83 399 24 873 362 1443
Cuts at: 4095 5053 442
Size: 958 1753
Fragments arranged by size:
1753 1443 958 873 416 399 362 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5024 1239
Size: 3785 2579
Bpu10I CC'TnA_GC
Cuts at: 2381 2460 3319 2381
Size: 79 859 5426
Fragments arranged by size:
5426 859 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2816 4489 5354 5652 2816
Size: 1673 865 298 3528
Fragments arranged by size:
3528 1673 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2820 5027 882
Size: 617 1321 2207 2219
Fragments arranged by size:
2219 2207 1321 617
BsaAI yAC'GTr
Cuts at: 493 1204 4155 493
Size: 711 2951 2702
Fragments arranged by size:
2951 2702 711
BsaBI GATnn'nnATC
Cuts at: 6358 6358
Size: 6364
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4171 4553 5188 275
Size: 53 83 186 936 2638 382 635 1451
Fragments arranged by size:
2638 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4797 5195 5469 5616 6027 4797
Size: 398 274 147 411 5134
Fragments arranged by size:
5134 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6139
Fragments arranged by size:
6139 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2309 3638 1186
Size: 1123 1329 3912
Fragments arranged by size:
3912 1329 1123
BseYI C'CCAG_C
Cuts at: 2593 5567 6063 2593
Size: 2974 496 2894
Fragments arranged by size:
2974 2894 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2294 2903 1163
Size: 573 558 609 4624
Fragments arranged by size:
4624 609 573 558
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2203 3814 4575 5603 1540
Size: 275 186 202 1611 761 1028 2301
Fragments arranged by size:
2301 1611 1028 761 275 202 186
BsiHKAI G_wGCw'C
Cuts at: 2148 4425 4510 5581 2148
Size: 2277 85 1071 2931
Fragments arranged by size:
2931 2277 1071 85
BsmI GAATG_Cn'
Cuts at: 3875 3968 3875
Size: 93 6271
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2148 2238 2353 2858 3683 4425
Size: 399 233 125 90 115 505 825 742
Cuts at: 4425 4510 5581 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 825 742 505 399 233 125
115 90 85
BspEI T'CCGG_A
Cuts at: 6027 6027
Size: 6364
BspHI T'CATG_A
Cuts at: 2805 4253 5983 2805
Size: 1448 1730 3186
Fragments arranged by size:
3186 1730 1448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2061 2985 3719 844
Size: 1217 924 734 3489
Fragments arranged by size:
3489 1217 924 734
BsrBI CCG'CTC
Cuts at: 1394 1823 4251 1394
Size: 429 2428 3507
Fragments arranged by size:
3507 2428 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4859 5033 65
Size: 1183 3611 174 1396
Fragments arranged by size:
3611 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5008 5195 1700
Size: 110 3198 187 2869
Fragments arranged by size:
3198 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6364
BssHII G'CGCG_C
Cuts at: 1925 2316 1925
Size: 391 5973
BssSI C'ACGA_G
Cuts at: 4424 5436 4424
Size: 1012 5352
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6364
BstBI TT'CG_AA
Cuts at: 5168 5168
Size: 6364
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6364
BstXI CCAn_nnnn'nTGG
Cuts at: 3056 3056
Size: 6364
BstYI r'GATC_y
Cuts at: 1972 3084 3208 3694 4061 4446 4463 5915 6001
Size: 1112 124 486 367 385 17 1452 86
Cuts at: 6001 6013 6359 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1112 486 385 367 346 124
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2391 2466 3018 3132 513
Size: 552 939 387 75 552 114 3745
Fragments arranged by size:
3745 939 552 552 387 114 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3436 487
Size: 683 56 2210 3415
Fragments arranged by size:
3415 2210 683 56
BtsI GCAGTG_nn'
Cuts at: 2448 2540 3880 4674 4694 2448
Size: 92 1340 794 20 4118
Fragments arranged by size:
4118 1340 794 92 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6364
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6329
DraI TTT'AAA
Cuts at: 3367 3668 3367
Size: 301 6063
DraIII CAC_nnn'GTG
Cuts at: 2403 2403
Size: 6364
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5371 809
Size: 507 4055 1802
Fragments arranged by size:
4055 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2289 2334 2394
Size: 54 1425 50 275 18 459 45 60
Cuts at: 2394 2451 3015 3811 4700 6329 8
Size: 57 564 796 889 1629 43
Fragments arranged by size:
1629 1425 889 796 564 459 275 60
57 54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3811 1537
Size: 275 1999 4090
Fragments arranged by size:
4090 1999 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2216 2421 2549 3296 4294 1353
Size: 863 205 128 747 998 3423
Fragments arranged by size:
3423 998 863 747 205 128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2138 4951 5325 5471 387
Size: 1064 138 549 2813 374 146 1280
Fragments arranged by size:
2813 1280 1064 549 374 146 138
EcoICRI GAG'CTC
Cuts at: 2146 2146
Size: 6364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2705 3233 3414 4427 5024 5811 6079
Size: 393 1073 528 181 1013 597 787 268
Cuts at: 6079 1239
Size: 1524
Fragments arranged by size:
1524 1073 1013 787 597 528 393 268
181
EcoNI CCTnn'n_nnAGG
Cuts at: 2384 2384
Size: 6364
EcoO109I rG'GnC_Cy
Cuts at: 2019 2020 2300 2019
Size: 1 280 6083
Fragments arranged by size:
6083 280 1
EcoRI G'AATT_C
Cuts at: 3673 4051 3673
Size: 378 5986
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2746 3532 2746
Size: 786 5578
FspI TGC'GCA
Cuts at: 4870 4870
Size: 6364
HaeII r_GCGC'y
Cuts at: 1678 2514 5511 1678
Size: 836 2997 2531
Fragments arranged by size:
2997 2531 836
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3455 3487 5005 5037 5079
Size: 32 135 32 1883 32 1518 32 42
Cuts at: 5079 5111 1373
Size: 32 2626
Fragments arranged by size:
2626 1883 1518 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3708 3954 669
Size: 988 281 1770 246 3079
Fragments arranged by size:
3079 1770 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6364
HpaI GTT'AAC
Cuts at: 3954 3954
Size: 6364
KpnI G_GTAC'C
Cuts at: 3689 3689
Size: 6364
MfeI C'AATT_G
Cuts at: 3963 3963
Size: 6364
MluI A'CGCG_T
Cuts at: 2858 6240 2858
Size: 3382 2982
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5478 5662 1305
Size: 4173 184 2007
Fragments arranged by size:
4173 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2336 2396 2453 10
Size: 54 1425 343 504 60 57 3921
Fragments arranged by size:
3921 1425 504 343 60 57 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2925 4322 4681 4840 518
Size: 648 1759 1397 359 159 2042
Fragments arranged by size:
2042 1759 1397 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2098 3780 4457 5605 5850 1606
Size: 492 1682 677 1148 245 2120
Fragments arranged by size:
2120 1682 1148 677 492 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2466 3132 513
Size: 552 939 462 666 3745
Fragments arranged by size:
3745 939 666 552 462
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6364
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6364
NotI GC'GGCC_GC
Cuts at: 3811 3811
Size: 6364
NspI r_CATG'y
Cuts at: 1132 1229 3722 5267 1132
Size: 97 2493 1545 2229
Fragments arranged by size:
2493 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6364
PciI A'CATG_T
Cuts at: 5263 5263
Size: 6364
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2392 1108
Size: 1284 5080
PmeI GTTT'AAAC
Cuts at: 3668 3668
Size: 6364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4433 4465 5946 5978 4433
Size: 32 1481 32 4819
Fragments arranged by size:
4819 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2300 2300
Size: 6364
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6364
PsiI TTA'TAA
Cuts at: 3934 3934
Size: 6364
PspOMI G'GGCC_C
Cuts at: 2019 2019
Size: 6364
PstI C_TGCA'G
Cuts at: 830 2058 2097 2133 2325 2443 2535 2996 3716
Size: 1228 39 36 192 118 92 461 720
Cuts at: 3716 830
Size: 3478
Fragments arranged by size:
3478 1228 720 461 192 118 92 39
36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6364
SacI G_AGCT'C
Cuts at: 2148 2148
Size: 6364
SalI G'TCGA_C
Cuts at: 1936 3706 1936
Size: 1770 4594
SanDI GG'GwC_CC
Cuts at: 2300 2300
Size: 6364
SapI GCTCTTCn'nnn_
Cuts at: 2421 3296 2421
Size: 875 5489
SbfI CC_TGCA'GG
Cuts at: 2058 3716 2058
Size: 1658 4706
ScaI AGT'ACT
Cuts at: 2573 4612 6324 2573
Size: 2039 1712 2613
Fragments arranged by size:
2613 2039 1712
SfcI C'TryA_G
Cuts at: 826 1045 2054 2093 2129 2321 2439 2531 2992
Size: 219 1009 39 36 192 118 92 461
Cuts at: 2992 3546 3712 4847 5528 5719 826
Size: 554 166 1135 681 191 1471
Fragments arranged by size:
1471 1135 1009 681 554 461 219 192
191 166 118 92 39 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6364
SmaI CCC'GGG
Cuts at: 1872 2125 2361 3691 1872
Size: 253 236 1330 4545
Fragments arranged by size:
4545 1330 253 236
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2831 4468 5369 5631 820
Size: 197 926 888 1637 901 262 1553
Fragments arranged by size:
1637 1553 926 901 888 262 197
SnaBI TAC'GTA
Cuts at: 493 4155 493
Size: 3662 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6364
SphI G_CATG'C
Cuts at: 1132 3722 1132
Size: 2590 3774
SrfI GCCC'GGGC
Cuts at: 2125 2125
Size: 6364
SspI AAT'ATT
Cuts at: 5 52 4288 5
Size: 47 4236 2081
Fragments arranged by size:
4236 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6364
StyI C'CwwG_G
Cuts at: 513 1065 2004 2454 2466 3132 3803 513
Size: 552 939 450 12 666 671 3074
Fragments arranged by size:
3074 939 671 666 552 450 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2189 3062 4391 4408 4561 4746 5191
Size: 1255 609 873 1329 17 153 185 445
Cuts at: 5191 325
Size: 1498
Fragments arranged by size:
1498 1329 1255 873 609 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2571 4610 6322
Size: 274 80 33 51 1443 594 2039 1712
Cuts at: 6322 96
Size: 138
Fragments arranged by size:
2039 1712 1443 594 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6324 6356 6324
Size: 32 6332
Tth111I GACn'n_nGTC
Cuts at: 1316 3236 1316
Size: 1920 4444
XbaI T'CTAG_A
Cuts at: 3700 3700
Size: 6364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2906 3070 2011
Size: 895 164 5305
Fragments arranged by size:
5305 895 164
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6364
XmaI C'CCGG_G
Cuts at: 1870 2123 2359 3689 1870
Size: 253 236 1330 4545
Fragments arranged by size:
4545 1330 253 236
XmnI GAAnn'nnTTC
Cuts at: 2543 4493 2543
Size: 1950 4414
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4172 276
Size: 53 83 186 936 2638 2468
Fragments arranged by size:
2638 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacII SexAI SfiI
SfoI SwaI