Restriction map for
pFN21AE0367
(Circular) (Six-base) MAPSORT of: pFN21AE0367.seq Check: 8655 from: 1 to: 6967
pFN21AE0367 6967 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4777 278
Size: 53 83 186 936 3241 2468
Fragments arranged by size:
3241 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4310 1937
Size: 2373 4594
Acc65I G'GTAC_C
Cuts at: 4288 4288
Size: 6967
AclI AA'CG_TT
Cuts at: 1563 5094 5467 1563
Size: 3531 373 3063
Fragments arranged by size:
3531 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2020 2492 3053 3571 5030 6414 6682 1632
Size: 388 472 561 518 1459 1384 268 1917
Fragments arranged by size:
1917 1459 1384 561 518 472 388 268
AfeI AGC'GCT
Cuts at: 2684 2684
Size: 6967
AflII C'TTAA_G
Cuts at: 820 1017 2435 820
Size: 197 1418 5352
Fragments arranged by size:
5352 1418 197
AflIII A'CryG_T
Cuts at: 1203 5866 6843 1203
Size: 4663 977 1327
Fragments arranged by size:
4663 1327 977
AgeI A'CCGG_T
Cuts at: 5798 5798
Size: 6967
AhdI GACnn_n'nnGTC
Cuts at: 2392 5696 2392
Size: 3304 3663
AleI CACnn'nnGTG
Cuts at: 1166 2317 1166
Size: 1151 5816
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2307 2778 1184
Size: 1123 471 5373
Fragments arranged by size:
5373 1123 471
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2999 3031 2999
Size: 32 6935
AlwNI CAG_nnn'CTG
Cuts at: 4120 4710 6282 4120
Size: 590 1572 4805
Fragments arranged by size:
4805 1572 590
ApaLI G'TGCA_C
Cuts at: 5024 6180 5024
Size: 1156 5811
ApoI r'AATT_y
Cuts at: 1493 2673 2701 2824 2920 3780 4276 4502 4654
Size: 1180 28 123 96 860 496 226 152
Cuts at: 4654 6763 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1180 860 496 226 152 123
96 28
AseI AT'TA_AT
Cuts at: 160 1032 5521 160
Size: 872 4489 1606
Fragments arranged by size:
4489 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6967
AvaI C'yCGr_G
Cuts at: 1870 1943 2184 4292 1870
Size: 73 241 2108 4545
Fragments arranged by size:
4545 2108 241 73
AvrII C'CTAG_G
Cuts at: 3787 3787
Size: 6967
BamHI G'GATC_C
Cuts at: 4297 4664 4297
Size: 367 6600
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4288 5743 618
Size: 325 217 627 2501 1455 1842
Fragments arranged by size:
2501 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4286 1391
Size: 2895 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2450 2906 3571 928
Size: 971 551 456 665 4324
Fragments arranged by size:
4324 971 665 551 456
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5158 5192 1903
Size: 34 3221 34 3678
Fragments arranged by size:
3678 3221 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2346 4859 6069 2346
Size: 2513 1210 3244
Fragments arranged by size:
3244 2513 1210
BclI T'GATC_A
Cuts at: 1548 2085 2124 1548
Size: 537 39 6391
Fragments arranged by size:
6391 537 39
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5578 136
Size: 107 122 71 631 605 3906 1525
Fragments arranged by size:
3906 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3323 6962 3323
Size: 3639 3328
BlpI GC'TnA_GC
Cuts at: 4384 4384
Size: 6967
Bme1580I G_kGCm'C
Cuts at: 1790 2767 5028 6184 1790
Size: 977 2261 1156 2573
Fragments arranged by size:
2573 2261 1156 977
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6967
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2794 4698 5656
Size: 416 53 83 399 24 1377 1904 958
Cuts at: 5656 442
Size: 1753
Fragments arranged by size:
1904 1753 1377 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6967
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4216 4216
Size: 6967
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5627 1239
Size: 4388 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2097 5092 5957 6255 2097
Size: 2995 865 298 2809
Fragments arranged by size:
2995 2809 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5630 882
Size: 617 4131 2219
Fragments arranged by size:
4131 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4758 493
Size: 711 3554 2702
Fragments arranged by size:
3554 2702 711
BsaBI GATnn'nnATC
Cuts at: 2658 6961 2658
Size: 4303 2664
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4774 5156 5791 275
Size: 53 83 186 936 3241 382 635 1451
Fragments arranged by size:
3241 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5400 5798 6072 6219 6630 5400
Size: 398 274 147 411 5737
Fragments arranged by size:
5737 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6742
Fragments arranged by size:
6742 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4189 1186
Size: 3003 3964
BseYI C'CCAG_C
Cuts at: 6170 6666 6170
Size: 496 6471
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3503 4106 1163
Size: 573 1767 603 4024
Fragments arranged by size:
4024 1767 603 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4417 5178 6206 1540
Size: 275 186 2416 761 1028 2301
Fragments arranged by size:
2416 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5028 5113 6184 5028
Size: 85 1071 5811
Fragments arranged by size:
5811 1071 85
BsmI GAATG_Cn'
Cuts at: 4478 4571 4478
Size: 93 6874
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2767 4286 5028 5113 6184 1391
Size: 399 977 1519 742 85 1071 2174
Fragments arranged by size:
2174 1519 1071 977 742 399 85
BspEI T'CCGG_A
Cuts at: 6630 6630
Size: 6967
BspHI T'CATG_A
Cuts at: 4856 6586 4856
Size: 1730 5237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3299 4322 844
Size: 2455 1023 3489
Fragments arranged by size:
3489 2455 1023
BsrBI CCG'CTC
Cuts at: 1394 1823 4854 1394
Size: 429 3031 3507
Fragments arranged by size:
3507 3031 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5462 5636 65
Size: 1183 4214 174 1396
Fragments arranged by size:
4214 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5611 5798 1700
Size: 110 3801 187 2869
Fragments arranged by size:
3801 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6967
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6967
BssSI C'ACGA_G
Cuts at: 5027 6039 5027
Size: 1012 5955
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6967
BstBI TT'CG_AA
Cuts at: 5771 5771
Size: 6967
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6967
BstYI r'GATC_y
Cuts at: 1972 2935 3323 4297 4664 5049 5066 6518 6604
Size: 963 388 974 367 385 17 1452 86
Cuts at: 6604 6616 6962 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 974 963 388 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6415
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6228
Fragments arranged by size:
6228 683 56
BtsI GCAGTG_nn'
Cuts at: 3491 4483 5277 5297 3491
Size: 992 794 20 5161
Fragments arranged by size:
5161 992 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6967
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6932
DraI TTT'AAA
Cuts at: 2026 2980 4271 2026
Size: 954 1291 4722
Fragments arranged by size:
4722 1291 954
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2428 5974 809
Size: 507 1112 3546 1802
Fragments arranged by size:
3546 1802 1112 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3507 4414 5303
Size: 54 1425 50 275 18 1677 907 889
Cuts at: 5303 6932 8
Size: 1629 43
Fragments arranged by size:
1677 1629 1425 907 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4414 1537
Size: 275 2602 4090
Fragments arranged by size:
4090 2602 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2039 2229 2751 3892 3995 4207 4897 1353
Size: 686 190 522 1141 103 212 690 3423
Fragments arranged by size:
3423 1141 690 686 522 212 190 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2243 5554 5928 6074 387
Size: 1064 138 654 3311 374 146 1280
Fragments arranged by size:
3311 1280 1064 654 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 2492 3053 3571 5030 5627 6414
Size: 393 388 472 561 518 1459 597 787
Cuts at: 6414 6682 1239
Size: 268 1524
Fragments arranged by size:
1524 1459 787 597 561 518 472 393
388 268
EcoO109I rG'GnC_Cy
Cuts at: 3235 3235
Size: 6967
EcoRI G'AATT_C
Cuts at: 4276 4654 4276
Size: 378 6589
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3478 3478
Size: 6967
FspI TGC'GCA
Cuts at: 5473 5473
Size: 6967
HaeII r_GCGC'y
Cuts at: 1678 2686 6114 1678
Size: 1008 3428 2531
Fragments arranged by size:
3428 2531 1008
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2378 2410 5608 5640 5682
Size: 32 135 32 806 32 3198 32 42
Cuts at: 5682 5714 1373
Size: 32 2626
Fragments arranged by size:
3198 2626 806 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4051 4311 4557 669
Size: 988 281 2113 260 246 3079
Fragments arranged by size:
3079 2113 988 281 260 246
HindIII A'AGCT_T
Cuts at: 748 2990 748
Size: 2242 4725
HpaI GTT'AAC
Cuts at: 4051 4557 4051
Size: 506 6461
KpnI G_GTAC'C
Cuts at: 4292 4292
Size: 6967
MfeI C'AATT_G
Cuts at: 4566 4566
Size: 6967
MluI A'CGCG_T
Cuts at: 6843 6843
Size: 6967
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6081 6265 1305
Size: 4776 184 2007
Fragments arranged by size:
4776 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3509 10
Size: 54 1425 343 1677 3468
Fragments arranged by size:
3468 1677 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2199 2289 2317 3116 3317 3641 3818
Size: 648 1033 90 28 799 201 324 177
Cuts at: 3818 4925 5284 5443 518
Size: 1107 359 159 2042
Fragments arranged by size:
2042 1107 1033 799 648 359 324 201
177 159 90 28
MspA1I CmG'CkG
Cuts at: 1606 4117 4383 5060 6208 6453 1606
Size: 2511 266 677 1148 245 2120
Fragments arranged by size:
2511 2120 1148 677 266 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6415
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6967
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6967
NotI GC'GGCC_GC
Cuts at: 4414 4414
Size: 6967
NspI r_CATG'y
Cuts at: 1132 1229 4325 5870 1132
Size: 97 3096 1545 2229
Fragments arranged by size:
3096 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6967
PciI A'CATG_T
Cuts at: 5866 5866
Size: 6967
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3954 4185 1108
Size: 2846 231 3890
Fragments arranged by size:
3890 2846 231
PfoI T'CCnGG_A
Cuts at: 2534 4022 2534
Size: 1488 5479
PmeI GTTT'AAAC
Cuts at: 4271 4271
Size: 6967
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5036 5068 6549 6581 5036
Size: 32 1481 32 5422
Fragments arranged by size:
5422 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3235 3235
Size: 6967
PshAI GACnn'nnGTC
Cuts at: 1613 3620 1613
Size: 2007 4960
PsiI TTA'TAA
Cuts at: 4537 4537
Size: 6967
PstI C_TGCA'G
Cuts at: 830 4319 830
Size: 3489 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6967
PvuII CAG'CTG
Cuts at: 4117 4117
Size: 6967
SalI G'TCGA_C
Cuts at: 1936 4309 1936
Size: 2373 4594
SapI GCTCTTCn'nnn_
Cuts at: 2751 2751
Size: 6967
SbfI CC_TGCA'GG
Cuts at: 4319 4319
Size: 6967
ScaI AGT'ACT
Cuts at: 5215 6927 5215
Size: 1712 5255
SfcI C'TryA_G
Cuts at: 826 1045 4315 5450 6131 6322 826
Size: 219 3270 1135 681 191 1471
Fragments arranged by size:
3270 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6967
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6967
SmaI CCC'GGG
Cuts at: 1872 4294 1872
Size: 2422 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2112 2435 5071 5972 6234 820
Size: 197 926 169 323 2636 901 262 1553
Fragments arranged by size:
2636 1553 926 901 323 262 197 169
SnaBI TAC'GTA
Cuts at: 493 4758 493
Size: 4265 2702
SpeI A'CTAG_T
Cuts at: 152 2252 152
Size: 2100 4867
SphI G_CATG'C
Cuts at: 1132 4325 1132
Size: 3193 3774
SspI AAT'ATT
Cuts at: 5 52 2571 2616 3264 4891 5
Size: 47 2519 45 648 1627 2081
Fragments arranged by size:
2519 2081 1627 648 47 45
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6967
StyI C'CwwG_G
Cuts at: 513 1065 3787 4406 513
Size: 552 2722 619 3074
Fragments arranged by size:
3074 2722 619 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2776 3386 4994 5011 5164 5349 5794
Size: 1255 1196 610 1608 17 153 185 445
Cuts at: 5794 325
Size: 1498
Fragments arranged by size:
1608 1498 1255 1196 610 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5213 6925 96
Size: 274 80 33 51 1443 3236 1712 138
Fragments arranged by size:
3236 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3344 3376 3888 3920 6927 6959 3344
Size: 32 512 32 3007 32 3352
Fragments arranged by size:
3352 3007 512 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6967
XbaI T'CTAG_A
Cuts at: 2650 3076 4303 2650
Size: 426 1227 5314
Fragments arranged by size:
5314 1227 426
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6967
XmaI C'CCGG_G
Cuts at: 1870 4292 1870
Size: 2422 4545
XmnI GAAnn'nnTTC
Cuts at: 2745 5096 2745
Size: 2351 4616
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4775 276
Size: 53 83 186 936 3241 2468
Fragments arranged by size:
3241 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI