Restriction map for
pFN21AE0364
(Circular) (Six-base) MAPSORT of: pFN21AE0364.seq Check: 6361 from: 1 to: 6775
pFN21AE0364 6775 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3712 3712
Size: 6775
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4585 278
Size: 53 83 186 936 3049 2468
Fragments arranged by size:
3049 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3297 4118 1937
Size: 1360 821 4594
Fragments arranged by size:
4594 1360 821
Acc65I G'GTAC_C
Cuts at: 4096 4096
Size: 6775
AclI AA'CG_TT
Cuts at: 1563 4902 5275 1563
Size: 3339 373 3063
Fragments arranged by size:
3339 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2330 2501 4838 6222 6490 1632
Size: 698 171 2337 1384 268 1917
Fragments arranged by size:
2337 1917 1384 698 268 171
AfeI AGC'GCT
Cuts at: 3269 3269
Size: 6775
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6578
AflIII A'CryG_T
Cuts at: 1203 2126 3687 3747 5674 6651 1203
Size: 923 1561 60 1927 977 1327
Fragments arranged by size:
1927 1561 1327 977 923 60
AgeI A'CCGG_T
Cuts at: 5606 5606
Size: 6775
AhdI GACnn_n'nnGTC
Cuts at: 5504 5504
Size: 6775
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6775
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6775
AlwNI CAG_nnn'CTG
Cuts at: 2087 2834 4518 6090 2087
Size: 747 1684 1572 2772
Fragments arranged by size:
2772 1684 1572 747
ApaI G_GGCC'C
Cuts at: 2479 2671 3355 4012 2479
Size: 192 684 657 5242
Fragments arranged by size:
5242 684 657 192
ApaLI G'TGCA_C
Cuts at: 4832 5988 4832
Size: 1156 5619
ApoI r'AATT_y
Cuts at: 1493 4084 4310 4462 6571 1493
Size: 2591 226 152 2109 1697
Fragments arranged by size:
2591 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5329 160
Size: 872 4297 1606
Fragments arranged by size:
4297 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6775
AvaI C'yCGr_G
Cuts at: 1870 1943 2454 2670 2890 3537 3656 4100 1870
Size: 73 511 216 220 647 119 444 4545
Fragments arranged by size:
4545 647 511 444 220 216 119 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3157 3190 3157
Size: 33 6742
BamHI G'GATC_C
Cuts at: 2119 3039 4105 4472 2119
Size: 920 1066 367 4422
Fragments arranged by size:
4422 1066 920 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2182 4096 5551 618
Size: 325 217 627 395 1914 1455 1842
Fragments arranged by size:
1914 1842 1455 627 395 325 217
BanII G_rGCy'C
Cuts at: 1391 2479 2663 2671 2794 3159 3355 4012 4094
Size: 1088 184 8 123 365 196 657 82
Cuts at: 4094 1391
Size: 4072
Fragments arranged by size:
4072 1088 657 365 196 184 123 82
8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3944 928
Size: 971 2045 3759
Fragments arranged by size:
3759 2045 971
BbvCI CC'TCA_GC
Cuts at: 2759 2759
Size: 6775
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4966 5000 1903
Size: 34 3029 34 3678
Fragments arranged by size:
3678 3029 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4667 5877 4667
Size: 1210 5565
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6775
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2077 2191 2239
Size: 107 122 71 631 605 405 114 48
Cuts at: 2239 2465 2483 5386 136
Size: 226 18 2903 1525
Fragments arranged by size:
2903 1525 631 605 405 226 122 114
107 71 48 18
BglII A'GATC_T
Cuts at: 3055 6770 3055
Size: 3715 3060
BlpI GC'TnA_GC
Cuts at: 2195 2487 2657 4192 2195
Size: 292 170 1535 4778
Fragments arranged by size:
4778 1535 292 170
Bme1580I G_kGCm'C
Cuts at: 1790 2187 2398 2479 2671 3355 4012 4836 5992
Size: 397 211 81 192 684 657 824 1156
Cuts at: 5992 1790
Size: 2573
Fragments arranged by size:
2573 1156 824 684 657 397 211 192
81
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6775
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2822 4006 4506
Size: 416 53 83 399 24 1405 1184 500
Cuts at: 4506 5464 442
Size: 958 1753
Fragments arranged by size:
1753 1405 1184 958 500 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3809 1056
Size: 2753 4022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2729 4087 5435 1239
Size: 1490 1358 1348 2579
Fragments arranged by size:
2579 1490 1358 1348
Bpu10I CC'TnA_GC
Cuts at: 2759 2759
Size: 6775
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2347 4900 5765 6063 2347
Size: 2553 865 298 3059
Fragments arranged by size:
3059 2553 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3248 5438 882
Size: 617 1749 2190 2219
Fragments arranged by size:
2219 2190 1749 617
BsaAI yAC'GTr
Cuts at: 493 1204 3019 4566 493
Size: 711 1815 1547 2702
Fragments arranged by size:
2702 1815 1547 711
BsaBI GATnn'nnATC
Cuts at: 6769 6769
Size: 6775
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3903 4582 4964 5599
Size: 53 83 186 936 2370 679 382 635
Cuts at: 5599 275
Size: 1451
Fragments arranged by size:
2370 1451 936 679 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3198 5208 5606 5880 6027 6438 3198
Size: 2010 398 274 147 411 3535
Fragments arranged by size:
3535 2010 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2211 2241 3698 3728 3824
Size: 30 165 30 808 30 1457 30 96
Cuts at: 3824 3854 1178
Size: 30 4099
Fragments arranged by size:
4099 1457 808 165 96 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2726 3365 1186
Size: 1540 639 4596
Fragments arranged by size:
4596 1540 639
BseYI C'CCAG_C
Cuts at: 2491 2578 2593 2849 3183 5978 6474 2491
Size: 87 15 256 334 2795 496 2792
Fragments arranged by size:
2795 2792 496 334 256 87 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3425 3821 1163
Size: 573 1689 396 4117
Fragments arranged by size:
4117 1689 573 396
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3652 4225 4986 6014 1540
Size: 275 186 1651 573 761 1028 2301
Fragments arranged by size:
2301 1651 1028 761 573 275 186
BsiHKAI G_wGCw'C
Cuts at: 2377 2663 3159 4836 4921 5992 2377
Size: 286 496 1677 85 1071 3160
Fragments arranged by size:
3160 1677 1071 496 286 85
BsmI GAATG_Cn'
Cuts at: 3387 4286 4379 3387
Size: 899 93 5783
Fragments arranged by size:
5783 899 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 2377 2398 2479 2663 2671 2794
Size: 399 397 190 21 81 184 8 123
Cuts at: 2794 3159 3355 4012 4094 4836 4921 5992 1391
Size: 365 196 657 82 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 657 399 397 365 196
190 184 123 85 82 81 21 8
BspEI T'CCGG_A
Cuts at: 6438 6438
Size: 6775
BspHI T'CATG_A
Cuts at: 4664 6394 4664
Size: 1730 5045
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2850 3712 4130 844
Size: 2006 862 418 3489
Fragments arranged by size:
3489 2006 862 418
BsrBI CCG'CTC
Cuts at: 1394 1823 2368 2935 4662 1394
Size: 429 545 567 1727 3507
Fragments arranged by size:
3507 1727 567 545 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3228 3235 5270 5444 65
Size: 1183 1980 7 2035 174 1396
Fragments arranged by size:
2035 1980 1396 1183 174 7
BsrFI r'CCGG_y
Cuts at: 1700 1810 2266 2331 2423 5419 5606 1700
Size: 110 456 65 92 2996 187 2869
Fragments arranged by size:
2996 2869 456 187 110 92 65
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6775
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6775
BssSI C'ACGA_G
Cuts at: 2376 3698 4835 5847 2376
Size: 1322 1137 1012 3304
Fragments arranged by size:
3304 1322 1137 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2653 794
Size: 1859 4916
BstBI TT'CG_AA
Cuts at: 5579 5579
Size: 6775
BstEII G'GTnAC_C
Cuts at: 1184 2495 3913 1184
Size: 1311 1418 4046
Fragments arranged by size:
4046 1418 1311
BstXI CCAn_nnnn'nTGG
Cuts at: 2610 2610
Size: 6775
BstYI r'GATC_y
Cuts at: 1972 2119 2292 2715 3039 3055 3285 4105 4472
Size: 147 173 423 324 16 230 820 367
Cuts at: 4472 4857 4874 6326 6412 6424 6770 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 820 423 385 367 346 324
230 173 147 86 17 16 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2188 3529 3618 3906 513
Size: 552 939 184 1341 89 288 3382
Fragments arranged by size:
3382 1341 939 552 288 184 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6036
Fragments arranged by size:
6036 683 56
BtsI GCAGTG_nn'
Cuts at: 4291 5085 5105 4291
Size: 794 20 5961
Fragments arranged by size:
5961 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6775
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6740
DraI TTT'AAA
Cuts at: 4079 4079
Size: 6775
DraIII CAC_nnn'GTG
Cuts at: 2653 2830 3703 2653
Size: 177 873 5725
Fragments arranged by size:
5725 873 177
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5782 809
Size: 507 4466 1802
Fragments arranged by size:
4466 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2078 2148 2286
Size: 54 1425 50 275 18 248 70 138
Cuts at: 2286 2586 2626 3615 3814 4222 5111 6740 8
Size: 300 40 989 199 408 889 1629 43
Fragments arranged by size:
1629 1425 989 889 408 300 275 248
199 138 70 54 50 43 40 18
EagI C'GGCC_G
Cuts at: 1537 1812 4222 1537
Size: 275 2410 4090
Fragments arranged by size:
4090 2410 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2882 4705 1353
Size: 1529 1823 3423
Fragments arranged by size:
3423 1823 1529
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3203 3440 5362 5736 5882 387
Size: 1064 138 1614 237 1922 374 146 1280
Fragments arranged by size:
1922 1614 1280 1064 374 237 146 138
EcoICRI GAG'CTC
Cuts at: 2661 3157 2661
Size: 496 6279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2330 2501 2729 4087 4838 5435 6222
Size: 393 698 171 228 1358 751 597 787
Cuts at: 6222 6490 1239
Size: 268 1524
Fragments arranged by size:
1524 1358 787 751 698 597 393 268
228 171
EcoNI CCTnn'n_nnAGG
Cuts at: 2778 2778
Size: 6775
EcoO109I rG'GnC_Cy
Cuts at: 2059 2414 2476 2667 3351 3352 2059
Size: 355 62 191 684 1 5482
Fragments arranged by size:
5482 684 355 191 62 1
EcoRI G'AATT_C
Cuts at: 4084 4462 4084
Size: 378 6397
FspI TGC'GCA
Cuts at: 3566 5281 3566
Size: 1715 5060
HaeII r_GCGC'y
Cuts at: 1678 2819 3271 3346 5922 1678
Size: 1141 452 75 2576 2531
Fragments arranged by size:
2576 2531 1141 452 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3427 3459 3674 3706 5416
Size: 32 135 32 1855 32 215 32 1710
Cuts at: 5416 5448 5490 5522 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1855 1710 215 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3331 3745 3982 4119 4365 669
Size: 988 281 1393 414 237 137 246 3079
Fragments arranged by size:
3079 1393 988 414 281 246 237 137
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6775
HpaI GTT'AAC
Cuts at: 3745 4365 3745
Size: 620 6155
KpnI G_GTAC'C
Cuts at: 4100 4100
Size: 6775
MfeI C'AATT_G
Cuts at: 4374 4374
Size: 6775
MluI A'CGCG_T
Cuts at: 6651 6651
Size: 6775
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3528 5889 6073 1305
Size: 2223 2361 184 2007
Fragments arranged by size:
2361 2223 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2080 2150 2288 2588 3617
Size: 54 1425 343 248 70 138 300 1029
Cuts at: 3617 10
Size: 3168
Fragments arranged by size:
3168 1425 1029 343 300 248 138 70
54
MslI CAynn'nnrTG
Cuts at: 518 1166 4733 5092 5251 518
Size: 648 3567 359 159 2042
Fragments arranged by size:
3567 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2207 2748 2981 3531 4191 4868 6016 6261
Size: 601 541 233 550 660 677 1148 245
Cuts at: 6261 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 660 601 550 541 245
233
NaeI GCC'GGC
Cuts at: 2268 2333 2425 2268
Size: 65 92 6618
Fragments arranged by size:
6618 92 65
NcoI C'CATG_G
Cuts at: 513 1065 2004 2188 3618 3906 513
Size: 552 939 184 1430 288 3382
Fragments arranged by size:
3382 1430 939 552 288 184
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6775
NgoMIV G'CCGG_C
Cuts at: 2266 2331 2423 2266
Size: 65 92 6618
Fragments arranged by size:
6618 92 65
NheI G'CTAG_C
Cuts at: 1052 3805 1052
Size: 2753 4022
NotI GC'GGCC_GC
Cuts at: 4222 4222
Size: 6775
NspI r_CATG'y
Cuts at: 1132 1229 2130 4133 5678 1132
Size: 97 901 2003 1545 2229
Fragments arranged by size:
2229 2003 1545 901 97
PasI CC'CwG_GG
Cuts at: 1348 2178 3355 1348
Size: 830 1177 4768
Fragments arranged by size:
4768 1177 830
PciI A'CATG_T
Cuts at: 2126 5674 2126
Size: 3548 3227
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3022 1108
Size: 1914 4861
PfoI T'CCnGG_A
Cuts at: 2697 3897 2697
Size: 1200 5575
PmeI GTTT'AAAC
Cuts at: 4079 4079
Size: 6775
PmlI CAC'GTG
Cuts at: 3019 3019
Size: 6775
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4844 4876 6357 6389 4844
Size: 32 1481 32 5230
Fragments arranged by size:
5230 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6775
PsiI TTA'TAA
Cuts at: 4345 4345
Size: 6775
PspOMI G'GGCC_C
Cuts at: 2475 2667 3351 4008 2475
Size: 192 684 657 5242
Fragments arranged by size:
5242 684 657 192
PstI C_TGCA'G
Cuts at: 830 2733 2946 4127 830
Size: 1903 213 1181 3478
Fragments arranged by size:
3478 1903 1181 213
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6775
PvuII CAG'CTG
Cuts at: 2207 2981 2207
Size: 774 6001
SacI G_AGCT'C
Cuts at: 2663 3159 2663
Size: 496 6279
SacII CC_GC'GG
Cuts at: 3532 3532
Size: 6775
SalI G'TCGA_C
Cuts at: 1936 4117 1936
Size: 2181 4594
SbfI CC_TGCA'GG
Cuts at: 4127 4127
Size: 6775
ScaI AGT'ACT
Cuts at: 5023 6735 5023
Size: 1712 5063
SfcI C'TryA_G
Cuts at: 826 1045 2729 2942 3462 4123 5258 5939 6130
Size: 219 1684 213 520 661 1135 681 191
Cuts at: 6130 826
Size: 1471
Fragments arranged by size:
1684 1471 1135 681 661 520 219 213
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6775
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6775
SmaI CCC'GGG
Cuts at: 1872 2456 2672 4102 1872
Size: 584 216 1430 4545
Fragments arranged by size:
4545 1430 584 216
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2326 4879 5780 6042 820
Size: 197 926 383 2553 901 262 1553
Fragments arranged by size:
2553 1553 926 901 383 262 197
SnaBI TAC'GTA
Cuts at: 493 4566 493
Size: 4073 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6775
SphI G_CATG'C
Cuts at: 1132 4133 1132
Size: 3001 3774
SrfI GCCC'GGGC
Cuts at: 2456 2456
Size: 6775
SspI AAT'ATT
Cuts at: 5 52 4699 5
Size: 47 4647 2081
Fragments arranged by size:
4647 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6775
StyI C'CwwG_G
Cuts at: 513 1065 2004 2188 3495 3618 3906 4214 513
Size: 552 939 184 1307 123 288 308 3074
Fragments arranged by size:
3074 1307 939 552 308 288 184 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 2170 2483 2841 3263 4802 4819
Size: 1255 417 173 313 358 422 1539 17
Cuts at: 4819 4972 5157 5602 325
Size: 153 185 445 1498
Fragments arranged by size:
1539 1498 1255 445 422 417 358 313
185 173 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3994 5021 6733
Size: 274 80 33 51 1443 2017 1027 1712
Cuts at: 6733 96
Size: 138
Fragments arranged by size:
2017 1712 1443 1027 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2211 2243 3195 3227 6735 6767 2211
Size: 32 952 32 3508 32 2219
Fragments arranged by size:
3508 2219 952 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6775
XbaI T'CTAG_A
Cuts at: 4111 4111
Size: 6775
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2256 3073 3700 2256
Size: 817 627 5331
Fragments arranged by size:
5331 817 627
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6775
XmaI C'CCGG_G
Cuts at: 1870 2454 2670 4100 1870
Size: 584 216 1430 4545
Fragments arranged by size:
4545 1430 584 216
XmnI GAAnn'nnTTC
Cuts at: 4904 4904
Size: 6775
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4583 276
Size: 53 83 186 936 3049 2468
Fragments arranged by size:
3049 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PpuMI PspXI PsrI RsrII SanDI
SapI SexAI SfiI SfoI SwaI