Restriction map for
pFN21AE0360
(Circular) (Six-base) MAPSORT of: pFN21AE0360.seq Check: 6205 from: 1 to: 6682
pFN21AE0360 6682 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4492 278
Size: 53 83 186 936 2956 2468
Fragments arranged by size:
2956 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2433 4025 1937
Size: 496 1592 4594
Fragments arranged by size:
4594 1592 496
Acc65I G'GTAC_C
Cuts at: 3043 4003 3043
Size: 960 5722
AclI AA'CG_TT
Cuts at: 1563 4809 5182 1563
Size: 3246 373 3063
Fragments arranged by size:
3246 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2168 2268 2984 4745 6129 6397 1632
Size: 536 100 716 1761 1384 268 1917
Fragments arranged by size:
1917 1761 1384 716 536 268 100
AfeI AGC'GCT
Cuts at: 2903 2903
Size: 6682
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6485
AflIII A'CryG_T
Cuts at: 1203 2922 3891 5581 6558 1203
Size: 1719 969 1690 977 1327
Fragments arranged by size:
1719 1690 1327 977 969
AgeI A'CCGG_T
Cuts at: 5513 5513
Size: 6682
AhdI GACnn_n'nnGTC
Cuts at: 2429 5411 2429
Size: 2982 3700
AleI CACnn'nnGTG
Cuts at: 1166 3110 1166
Size: 1944 4738
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6682
AlwNI CAG_nnn'CTG
Cuts at: 3552 4425 5997 3552
Size: 873 1572 4237
Fragments arranged by size:
4237 1572 873
ApaLI G'TGCA_C
Cuts at: 2878 3575 4739 5895 2878
Size: 697 1164 1156 3665
Fragments arranged by size:
3665 1164 1156 697
ApoI r'AATT_y
Cuts at: 1493 3633 3991 4217 4369 6478 1493
Size: 2140 358 226 152 2109 1697
Fragments arranged by size:
2140 2109 1697 358 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5236 160
Size: 872 4204 1606
Fragments arranged by size:
4204 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6682
AvaI C'yCGr_G
Cuts at: 1870 1943 2778 4007 1870
Size: 73 835 1229 4545
Fragments arranged by size:
4545 1229 835 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3656 3689 3656
Size: 33 6649
BamHI G'GATC_C
Cuts at: 4012 4379 4012
Size: 367 6315
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2216 3043 4003 5458 618
Size: 325 217 627 429 827 960 1455 1842
Fragments arranged by size:
1842 1455 960 827 627 429 325 217
BanII G_rGCy'C
Cuts at: 1391 3182 3270 4001 1391
Size: 1791 88 731 4072
Fragments arranged by size:
4072 1791 731 88
BbeI G_GCGC'C
Cuts at: 2220 2220
Size: 6682
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2156 928
Size: 971 257 5454
Fragments arranged by size:
5454 971 257
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3707 3741 4873 4907 1903
Size: 34 1770 34 1132 34 3678
Fragments arranged by size:
3678 1770 1132 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4574 5784 4574
Size: 1210 5472
BclI T'GATC_A
Cuts at: 1548 3003 1548
Size: 1455 5227
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2913 5293 136
Size: 107 122 71 631 605 1241 2380 1525
Fragments arranged by size:
2380 1525 1241 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6677 6677
Size: 6682
BlpI GC'TnA_GC
Cuts at: 4099 4099
Size: 6682
Bme1580I G_kGCm'C
Cuts at: 1790 2882 3579 4743 5899 1790
Size: 1092 697 1164 1156 2573
Fragments arranged by size:
2573 1164 1156 1092 697
BmgBI CAC'GTC
Cuts at: 1318 3894 1318
Size: 2576 4106
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2595 2826 3540
Size: 416 53 83 399 24 1178 231 714
Cuts at: 3540 4413 5371 442
Size: 873 958 1753
Fragments arranged by size:
1753 1178 958 873 714 416 399 231
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6682
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3922 3922
Size: 6682
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2411 2517 2606 5342 1239
Size: 1172 106 89 2736 2579
Fragments arranged by size:
2736 2579 1172 106 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2384 3785 3892 4807 5672 5970 2384
Size: 1401 107 915 865 298 3096
Fragments arranged by size:
3096 1401 915 865 298 107
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2033 5345 882
Size: 617 534 3312 2219
Fragments arranged by size:
3312 2219 617 534
BsaAI yAC'GTr
Cuts at: 493 1204 2713 2932 3791 4473 493
Size: 711 1509 219 859 682 2702
Fragments arranged by size:
2702 1509 859 711 682 219
BsaBI GATnn'nnATC
Cuts at: 3666 6676 3666
Size: 3010 3672
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2217 4489 4871 5506
Size: 53 83 186 936 684 2272 382 635
Cuts at: 5506 275
Size: 1451
Fragments arranged by size:
2272 1451 936 684 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5115 5513 5787 5934 6345 5115
Size: 398 274 147 411 5452
Fragments arranged by size:
5452 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6457
Fragments arranged by size:
6457 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3722 3872 3875 3932 1186
Size: 2536 150 3 57 3936
Fragments arranged by size:
3936 2536 150 57 3
BseYI C'CCAG_C
Cuts at: 2024 2267 3684 5885 6381 2024
Size: 243 1417 2201 496 2325
Fragments arranged by size:
2325 2201 1417 496 243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2918 3070 1163
Size: 573 1182 152 4775
Fragments arranged by size:
4775 1182 573 152
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4132 4893 5921 1540
Size: 275 186 2131 761 1028 2301
Fragments arranged by size:
2301 2131 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2882 3182 3270 3579 4743 4828 5899 2882
Size: 300 88 309 1164 85 1071 3665
Fragments arranged by size:
3665 1164 1071 309 300 88 85
BsmI GAATG_Cn'
Cuts at: 2722 2856 3059 3458 4193 4286 2722
Size: 134 203 399 735 93 5118
Fragments arranged by size:
5118 735 399 203 134 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2882 3182 3270 3579 4001 4743 4828
Size: 399 1092 300 88 309 422 742 85
Cuts at: 4828 5899 1391
Size: 1071 2174
Fragments arranged by size:
2174 1092 1071 742 422 399 309 300
88 85
BspEI T'CCGG_A
Cuts at: 6345 6345
Size: 6682
BspHI T'CATG_A
Cuts at: 3174 4571 6301 3174
Size: 1397 1730 3555
Fragments arranged by size:
3555 1730 1397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4037 844
Size: 3193 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4569 1394
Size: 429 2746 3507
Fragments arranged by size:
3507 2746 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3492 5177 5351 65
Size: 1183 2244 1685 174 1396
Fragments arranged by size:
2244 1685 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5326 5513 1700
Size: 110 3516 187 2869
Fragments arranged by size:
3516 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3018 3664 96
Size: 2922 646 3114
Fragments arranged by size:
3114 2922 646
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6682
BssSI C'ACGA_G
Cuts at: 3907 4742 5754 3907
Size: 835 1012 4835
Fragments arranged by size:
4835 1012 835
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6682
BstBI TT'CG_AA
Cuts at: 5486 5486
Size: 6682
BstEII G'GTnAC_C
Cuts at: 1184 3622 1184
Size: 2438 4244
BstYI r'GATC_y
Cuts at: 1972 2346 4012 4379 4764 4781 6233 6319 6331
Size: 374 1666 367 385 17 1452 86 12
Cuts at: 6331 6677 1972
Size: 346 1977
Fragments arranged by size:
1977 1666 1452 385 374 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2828 513
Size: 552 939 824 4367
Fragments arranged by size:
4367 939 824 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2156 487
Size: 683 56 930 5013
Fragments arranged by size:
5013 930 683 56
BtsI GCAGTG_nn'
Cuts at: 4198 4992 5012 4198
Size: 794 20 5868
Fragments arranged by size:
5868 794 20
ClaI AT'CG_AT
Cuts at: 1554 3717 1554
Size: 2163 4519
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2040 2075 529
Size: 35 1476 35 5136
Fragments arranged by size:
5136 1476 35 35
DraI TTT'AAA
Cuts at: 3986 3986
Size: 6682
DraIII CAC_nnn'GTG
Cuts at: 2257 2257
Size: 6682
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2093 5689 809
Size: 507 777 3596 1802
Fragments arranged by size:
3596 1802 777 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2207 2831
Size: 54 1425 50 275 18 177 200 624
Cuts at: 2831 4129 5018 6647 8
Size: 1298 889 1629 43
Fragments arranged by size:
1629 1425 1298 889 624 275 200 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4129 1537
Size: 275 2317 4090
Fragments arranged by size:
4090 2317 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2459 3221 3726 4612 1353
Size: 1106 762 505 886 3423
Fragments arranged by size:
3423 1106 886 762 505
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2798 5269 5643 5789 387
Size: 1064 138 1209 2471 374 146 1280
Fragments arranged by size:
2471 1280 1209 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3180 3268 3180
Size: 88 6594
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2268 2411 2517 2606 2984 4745
Size: 393 536 100 143 106 89 378 1761
Cuts at: 4745 5342 6129 6397 1239
Size: 597 787 268 1524
Fragments arranged by size:
1761 1524 787 597 536 393 378 268
143 106 100 89
EcoNI CCTnn'n_nnAGG
Cuts at: 3910 3910
Size: 6682
EcoO109I rG'GnC_Cy
Cuts at: 3325 3839 3903 3325
Size: 514 64 6104
Fragments arranged by size:
6104 514 64
EcoRI G'AATT_C
Cuts at: 3991 4369 3991
Size: 378 6304
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2536 2536
Size: 6682
FspI TGC'GCA
Cuts at: 5188 5188
Size: 6682
HaeII r_GCGC'y
Cuts at: 1678 2220 2905 3207 5829 1678
Size: 542 685 302 2622 2531
Fragments arranged by size:
2622 2531 685 542 302
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2415 2447 3345 3377 5323
Size: 32 135 32 843 32 898 32 1946
Cuts at: 5323 5355 5397 5429 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1946 898 843 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4026 4272 669
Size: 988 281 2088 246 3079
Fragments arranged by size:
3079 2088 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3807 748
Size: 3059 3623
HpaI GTT'AAC
Cuts at: 4272 4272
Size: 6682
KasI G'GCGC_C
Cuts at: 2216 2216
Size: 6682
KpnI G_GTAC'C
Cuts at: 3047 4007 3047
Size: 960 5722
MfeI C'AATT_G
Cuts at: 3402 4281 3402
Size: 879 5803
MluI A'CGCG_T
Cuts at: 6558 6558
Size: 6682
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2662 3237 3457 5796 5980 1305
Size: 1357 575 220 2339 184 2007
Fragments arranged by size:
2339 2007 1357 575 220 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2833 10
Size: 54 1425 343 177 824 3859
Fragments arranged by size:
3859 1425 824 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2308 2462 2633 2990 3026 3110 3239
Size: 648 1142 154 171 357 36 84 129
Cuts at: 3239 3500 3793 4640 4999 5158 518
Size: 261 293 847 359 159 2042
Fragments arranged by size:
2042 1142 847 648 359 357 293 261
171 159 154 129 84 36
MspA1I CmG'CkG
Cuts at: 1606 3410 4098 4775 5923 6168 1606
Size: 1804 688 677 1148 245 2120
Fragments arranged by size:
2120 1804 1148 688 677 245
NarI GG'CG_CC
Cuts at: 2217 2217
Size: 6682
NcoI C'CATG_G
Cuts at: 513 1065 2004 2828 513
Size: 552 939 824 4367
Fragments arranged by size:
4367 939 824 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6682
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6682
NotI GC'GGCC_GC
Cuts at: 4129 4129
Size: 6682
NspI r_CATG'y
Cuts at: 1132 1229 2926 3039 4040 5585 1132
Size: 97 1697 113 1001 1545 2229
Fragments arranged by size:
2229 1697 1545 1001 113 97
PasI CC'CwG_GG
Cuts at: 1348 3628 1348
Size: 2280 4402
PciI A'CATG_T
Cuts at: 2922 5581 2922
Size: 2659 4023
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2257 1108
Size: 1149 5533
PmeI GTTT'AAAC
Cuts at: 3986 3986
Size: 6682
PmlI CAC'GTG
Cuts at: 2932 3791 2932
Size: 859 5823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4751 4783 6264 6296 4751
Size: 32 1481 32 5137
Fragments arranged by size:
5137 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3325 3839 3325
Size: 514 6168
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6682
PsiI TTA'TAA
Cuts at: 4252 4252
Size: 6682
PstI C_TGCA'G
Cuts at: 830 2485 4034 830
Size: 1655 1549 3478
Fragments arranged by size:
3478 1655 1549
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6682
PvuII CAG'CTG
Cuts at: 3410 3410
Size: 6682
SacI G_AGCT'C
Cuts at: 3182 3270 3182
Size: 88 6594
SalI G'TCGA_C
Cuts at: 1936 4024 1936
Size: 2088 4594
SanDI GG'GwC_CC
Cuts at: 3325 3839 3325
Size: 514 6168
SbfI CC_TGCA'GG
Cuts at: 4034 4034
Size: 6682
ScaI AGT'ACT
Cuts at: 4930 6642 4930
Size: 1712 4970
SfcI C'TryA_G
Cuts at: 826 1045 2166 2481 4030 5165 5846 6037 826
Size: 219 1121 315 1549 1135 681 191 1471
Fragments arranged by size:
1549 1471 1135 1121 681 315 219 191
SfoI GGC'GCC
Cuts at: 2218 2218
Size: 6682
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6682
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6682
SmaI CCC'GGG
Cuts at: 1872 4009 1872
Size: 2137 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2399 3800 3871 4786 5687 5949
Size: 197 926 456 1401 71 915 901 262
Cuts at: 5949 820
Size: 1553
Fragments arranged by size:
1553 1401 926 915 901 456 262 197
71
SnaBI TAC'GTA
Cuts at: 493 4473 493
Size: 3980 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6682
SphI G_CATG'C
Cuts at: 1132 4040 1132
Size: 2908 3774
SspI AAT'ATT
Cuts at: 5 52 4606 5
Size: 47 4554 2081
Fragments arranged by size:
4554 2081 47
StuI AGG'CCT
Cuts at: 1517 3144 1517
Size: 1627 5055
StyI C'CwwG_G
Cuts at: 513 1065 2004 2520 2828 3130 3391 3852 4121
Size: 552 939 516 308 302 261 461 269
Cuts at: 4121 513
Size: 3074
Fragments arranged by size:
3074 939 552 516 461 308 302 269
261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2331 3145 4709 4726 4879 5064 5509
Size: 1255 751 814 1564 17 153 185 445
Cuts at: 5509 325
Size: 1498
Fragments arranged by size:
1564 1498 1255 814 751 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3018 3585 3664
Size: 274 80 33 51 1443 1041 567 79
Cuts at: 3664 3701 4928 6640 96
Size: 37 1227 1712 138
Fragments arranged by size:
1712 1443 1227 1041 567 274 138 80
79 51 37 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6642 6674 6642
Size: 32 6650
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6682
XbaI T'CTAG_A
Cuts at: 4018 4018
Size: 6682
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2590 2983 3112 3943 2590
Size: 393 129 831 5329
Fragments arranged by size:
5329 831 393 129
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6682
XmaI C'CCGG_G
Cuts at: 1870 4007 1870
Size: 2137 4545
XmnI GAAnn'nnTTC
Cuts at: 4811 4811
Size: 6682
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4490 276
Size: 53 83 186 936 2956 2468
Fragments arranged by size:
2956 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI
PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI