Restriction map for
pFN21AE0359
(Circular) (Six-base) MAPSORT of: pFN21AE0359.seq Check: 556 from: 1 to: 6451
pFN21AE0359 6451 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4261 278
Size: 53 83 186 936 2725 2468
Fragments arranged by size:
2725 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3794 1937
Size: 1857 4594
Acc65I G'GTAC_C
Cuts at: 2112 3772 2112
Size: 1660 4791
AclI AA'CG_TT
Cuts at: 1563 4578 4951 1563
Size: 3015 373 3063
Fragments arranged by size:
3063 3015 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4514 5898 6166 1632
Size: 2882 1384 268 1917
Fragments arranged by size:
2882 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6254
AflIII A'CryG_T
Cuts at: 1203 2538 5350 6327 1203
Size: 1335 2812 977 1327
Fragments arranged by size:
2812 1335 1327 977
AgeI A'CCGG_T
Cuts at: 5282 5282
Size: 6451
AhdI GACnn_n'nnGTC
Cuts at: 5180 5180
Size: 6451
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6451
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6451
AlwNI CAG_nnn'CTG
Cuts at: 2645 3557 4194 5766 2645
Size: 912 637 1572 3330
Fragments arranged by size:
3330 1572 912 637
ApaLI G'TGCA_C
Cuts at: 4508 5664 4508
Size: 1156 5295
ApoI r'AATT_y
Cuts at: 1493 2510 3394 3501 3545 3760 3986 4138 6247
Size: 1017 884 107 44 215 226 152 2109
Cuts at: 6247 1493
Size: 1697
Fragments arranged by size:
2109 1697 1017 884 226 215 152 107
44
AseI AT'TA_AT
Cuts at: 160 1032 5005 160
Size: 872 3973 1606
Fragments arranged by size:
3973 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6451
AvaI C'yCGr_G
Cuts at: 1870 1943 3776 1870
Size: 73 1833 4545
Fragments arranged by size:
4545 1833 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2768 2801 2768
Size: 33 6418
BamHI G'GATC_C
Cuts at: 3781 4148 3781
Size: 367 6084
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2064 2096 2112 2128 3772
Size: 325 217 627 277 32 16 16 1644
Cuts at: 3772 5227 618
Size: 1455 1842
Fragments arranged by size:
1842 1644 1455 627 325 277 217 32
16 16
BanII G_rGCy'C
Cuts at: 1391 2261 2286 3770 1391
Size: 870 25 1484 4072
Fragments arranged by size:
4072 1484 870 25
BbeI G_GCGC'C
Cuts at: 2132 2132
Size: 6451
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3468 928
Size: 971 1569 3911
Fragments arranged by size:
3911 1569 971
BbvCI CC'TCA_GC
Cuts at: 2192 2192
Size: 6451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4642 4676 1903
Size: 34 50 34 2621 34 3678
Fragments arranged by size:
3678 2621 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4343 5553 4343
Size: 1210 5241
BclI T'GATC_A
Cuts at: 1548 3595 1548
Size: 2047 4404
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2126 2223 5062
Size: 107 122 71 631 605 454 97 2839
Cuts at: 5062 136
Size: 1525
Fragments arranged by size:
2839 1525 631 605 454 122 107 97
71
BglII A'GATC_T
Cuts at: 6446 6446
Size: 6451
BlpI GC'TnA_GC
Cuts at: 3740 3868 3740
Size: 128 6323
Bme1580I G_kGCm'C
Cuts at: 1790 4512 5668 1790
Size: 2722 1156 2573
Fragments arranged by size:
2722 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6451
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3110 4182 5140
Size: 416 53 83 399 24 1693 1072 958
Cuts at: 5140 442
Size: 1753
Fragments arranged by size:
1753 1693 1072 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2667 2718 3339 5111 1239
Size: 1428 51 621 1772 2579
Fragments arranged by size:
2579 1772 1428 621 51
Bpu10I CC'TnA_GC
Cuts at: 2192 2192
Size: 6451
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2941 4576 5441 5739 2941
Size: 1635 865 298 3653
Fragments arranged by size:
3653 1635 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5114 882
Size: 617 3615 2219
Fragments arranged by size:
3615 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4242 493
Size: 711 3038 2702
Fragments arranged by size:
3038 2702 711
BsaBI GATnn'nnATC
Cuts at: 2820 6445 2820
Size: 3625 2826
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2129 4258 4640 5275
Size: 53 83 186 936 596 2129 382 635
Cuts at: 5275 275
Size: 1451
Fragments arranged by size:
2129 1451 936 635 596 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4884 5282 5556 5703 6114 4884
Size: 398 274 147 411 5221
Fragments arranged by size:
5221 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6226
Fragments arranged by size:
6226 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2127 2132 2266 2269 3201 1186
Size: 856 85 5 134 3 932 4436
Fragments arranged by size:
4436 932 856 134 85 5 3
BseYI C'CCAG_C
Cuts at: 5654 6150 5654
Size: 496 5955
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3096 1163
Size: 573 1360 4518
Fragments arranged by size:
4518 1360 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2213 3901 4662 5690 1540
Size: 275 186 212 1688 761 1028 2301
Fragments arranged by size:
2301 1688 1028 761 275 212 186
BsiHKAI G_wGCw'C
Cuts at: 4512 4597 5668 4512
Size: 85 1071 5295
Fragments arranged by size:
5295 1071 85
BsmI GAATG_Cn'
Cuts at: 3962 4055 3962
Size: 93 6358
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2261 2286 3770 4512 4597 5668 1391
Size: 399 471 25 1484 742 85 1071 2174
Fragments arranged by size:
2174 1484 1071 742 471 399 85 25
BspEI T'CCGG_A
Cuts at: 6114 6114
Size: 6451
BspHI T'CATG_A
Cuts at: 3457 4340 6070 3457
Size: 883 1730 3838
Fragments arranged by size:
3838 1730 883
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3806 844
Size: 2962 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4338 1394
Size: 429 2515 3507
Fragments arranged by size:
3507 2515 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4946 5120 65
Size: 1183 3698 174 1396
Fragments arranged by size:
3698 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2149 5095 5282 1700
Size: 110 339 2946 187 2869
Fragments arranged by size:
2946 2869 339 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6451
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6451
BssSI C'ACGA_G
Cuts at: 4511 5523 4511
Size: 1012 5439
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6451
BstBI TT'CG_AA
Cuts at: 5255 5255
Size: 6451
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6451
BstXI CCAn_nnnn'nTGG
Cuts at: 2891 2891
Size: 6451
BstYI r'GATC_y
Cuts at: 1972 3487 3781 4148 4533 4550 6002 6088 6100
Size: 1515 294 367 385 17 1452 86 12
Cuts at: 6100 6446 1972
Size: 346 1977
Fragments arranged by size:
1977 1515 1452 385 367 346 294 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2030 2030
Size: 6451
BtgI C'CryG_G
Cuts at: 513 1065 2165 2218 2286 513
Size: 552 1100 53 68 4678
Fragments arranged by size:
4678 1100 552 68 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5712
Fragments arranged by size:
5712 683 56
BtsI GCAGTG_nn'
Cuts at: 2466 3967 4761 4781 2466
Size: 1501 794 20 4136
Fragments arranged by size:
4136 1501 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6451
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6416
DraI TTT'AAA
Cuts at: 3755 3755
Size: 6451
DraIII CAC_nnn'GTG
Cuts at: 3422 3422
Size: 6451
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5458 809
Size: 507 4142 1802
Fragments arranged by size:
4142 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2210 2274 3898
Size: 54 1425 50 275 18 380 64 1624
Cuts at: 3898 4787 6416 8
Size: 889 1629 43
Fragments arranged by size:
1629 1624 1425 889 380 275 64 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2210 3898 1537
Size: 275 398 1688 4090
Fragments arranged by size:
4090 1688 398 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4381 1353
Size: 3028 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5038 5412 5558 387
Size: 1064 138 3449 374 146 1280
Fragments arranged by size:
3449 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2667 2718 3339 4514 5111 5898 6166
Size: 393 1035 51 621 1175 597 787 268
Cuts at: 6166 1239
Size: 1524
Fragments arranged by size:
1524 1175 1035 787 621 597 393 268
51
EcoNI CCTnn'n_nnAGG
Cuts at: 2910 2910
Size: 6451
EcoO109I rG'GnC_Cy
Cuts at: 2454 2454
Size: 6451
EcoRI G'AATT_C
Cuts at: 3545 3760 4138 3545
Size: 215 378 5858
Fragments arranged by size:
5858 378 215
FspI TGC'GCA
Cuts at: 3401 4957 3401
Size: 1556 4895
HaeII r_GCGC'y
Cuts at: 1678 2132 5598 1678
Size: 454 3466 2531
Fragments arranged by size:
3466 2531 454
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2473 2505 2525 2557 2978
Size: 32 135 32 901 32 20 32 421
Cuts at: 2978 3010 3071 3103 5092 5124 5166 5198 1373
Size: 32 61 32 1989 32 42 32 2626
Fragments arranged by size:
2626 1989 901 421 135 61 42 32
32 32 32 32 32 32 32 20
HincII GTy'rAC
Cuts at: 669 1657 1938 3795 4041 669
Size: 988 281 1857 246 3079
Fragments arranged by size:
3079 1857 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2844 748
Size: 2096 4355
HpaI GTT'AAC
Cuts at: 4041 4041
Size: 6451
KasI G'GCGC_C
Cuts at: 2128 2128
Size: 6451
KpnI G_GTAC'C
Cuts at: 2116 3776 2116
Size: 1660 4791
MfeI C'AATT_G
Cuts at: 4050 4050
Size: 6451
MluI A'CGCG_T
Cuts at: 6327 6327
Size: 6451
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2557 3203 5565 5749 1305
Size: 1252 646 2362 184 2007
Fragments arranged by size:
2362 2007 1252 646 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4629
Fragments arranged by size:
4629 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2496 2826 2829 2889 4409 4768 4927
Size: 648 1330 330 3 60 1520 359 159
Cuts at: 4927 518
Size: 2042
Fragments arranged by size:
2042 1520 1330 648 359 330 159 60
3
MspA1I CmG'CkG
Cuts at: 1606 2070 2167 2220 3867 4544 5692 5937 1606
Size: 464 97 53 1647 677 1148 245 2120
Fragments arranged by size:
2120 1647 1148 677 464 245 97 53
NarI GG'CG_CC
Cuts at: 2129 2129
Size: 6451
NcoI C'CATG_G
Cuts at: 513 1065 2286 513
Size: 552 1221 4678
Fragments arranged by size:
4678 1221 552
NdeI CA'TA_TG
Cuts at: 387 2998 3229 387
Size: 2611 231 3609
Fragments arranged by size:
3609 2611 231
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6451
NotI GC'GGCC_GC
Cuts at: 3898 3898
Size: 6451
NruI TCG'CGA
Cuts at: 2057 2057
Size: 6451
NspI r_CATG'y
Cuts at: 1132 1229 2542 3406 3809 5354 1132
Size: 97 1313 864 403 1545 2229
Fragments arranged by size:
2229 1545 1313 864 403 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6451
PciI A'CATG_T
Cuts at: 2538 5350 2538
Size: 2812 3639
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6451
PmeI GTTT'AAAC
Cuts at: 3755 3755
Size: 6451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4520 4552 6033 6065 4520
Size: 32 1481 32 4906
Fragments arranged by size:
4906 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6451
PsiI TTA'TAA
Cuts at: 4021 4021
Size: 6451
PstI C_TGCA'G
Cuts at: 830 3241 3803 830
Size: 2411 562 3478
Fragments arranged by size:
3478 2411 562
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6451
SacII CC_GC'GG
Cuts at: 2168 2221 2168
Size: 53 6398
SalI G'TCGA_C
Cuts at: 1936 3793 1936
Size: 1857 4594
SbfI CC_TGCA'GG
Cuts at: 3803 3803
Size: 6451
ScaI AGT'ACT
Cuts at: 4699 6411 4699
Size: 1712 4739
SexAI A'CCwGG_T
Cuts at: 3418 3418
Size: 6451
SfcI C'TryA_G
Cuts at: 826 1045 3237 3799 4934 5615 5806 826
Size: 219 2192 562 1135 681 191 1471
Fragments arranged by size:
2192 1471 1135 681 562 219 191
SfoI GGC'GCC
Cuts at: 2130 2130
Size: 6451
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6451
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6451
SmaI CCC'GGG
Cuts at: 1872 3778 1872
Size: 1906 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2920 4555 5456 5718 820
Size: 197 926 977 1635 901 262 1553
Fragments arranged by size:
1635 1553 977 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4242 493
Size: 3749 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6451
SphI G_CATG'C
Cuts at: 1132 3809 1132
Size: 2677 3774
SspI AAT'ATT
Cuts at: 5 52 4375 5
Size: 47 4323 2081
Fragments arranged by size:
4323 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6451
StyI C'CwwG_G
Cuts at: 513 1065 2286 3687 3890 513
Size: 552 1221 1401 203 3074
Fragments arranged by size:
3074 1401 1221 552 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4478 4495 4648 4833 5278 325
Size: 1255 2898 17 153 185 445 1498
Fragments arranged by size:
2898 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4697 6409 96
Size: 274 80 33 51 1443 2720 1712 138
Fragments arranged by size:
2720 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3408 3440 3520 3552 6411 6443 3408
Size: 32 80 32 2859 32 3416
Fragments arranged by size:
3416 2859 80 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2367 1316
Size: 1051 5400
XbaI T'CTAG_A
Cuts at: 3787 3787
Size: 6451
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6451
XmaI C'CCGG_G
Cuts at: 1870 3776 1870
Size: 1906 4545
XmnI GAAnn'nnTTC
Cuts at: 2901 4580 2901
Size: 1679 4772
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4259 276
Size: 53 83 186 936 2725 2468
Fragments arranged by size:
2725 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NruI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BfrBI BplI
BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI
NaeI NgoMIV NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SanDI SapI SfiI
SrfI SwaI XcmI