Restriction map for
pFN21AE0357
(Circular) (Six-base) MAPSORT of: pFN21AE0357.seq Check: 3998 from: 1 to: 6508
pFN21AE0357 6508 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4318 278
Size: 53 83 186 936 2782 2468
Fragments arranged by size:
2782 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3851 1937
Size: 1914 4594
Acc65I G'GTAC_C
Cuts at: 2280 3829 2280
Size: 1549 4959
AclI AA'CG_TT
Cuts at: 1563 4635 5008 1563
Size: 3072 373 3063
Fragments arranged by size:
3072 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2913 2974 2985 3228 3653 4571 5955 6223
Size: 1281 61 11 243 425 918 1384 268
Cuts at: 6223 1632
Size: 1917
Fragments arranged by size:
1917 1384 1281 918 425 268 243 61
11
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6311
AflIII A'CryG_T
Cuts at: 1203 3283 5407 6384 1203
Size: 2080 2124 977 1327
Fragments arranged by size:
2124 2080 1327 977
AgeI A'CCGG_T
Cuts at: 5339 5339
Size: 6508
AhdI GACnn_n'nnGTC
Cuts at: 5237 5237
Size: 6508
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6508
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6508
AlwNI CAG_nnn'CTG
Cuts at: 3593 4251 5823 3593
Size: 658 1572 4278
Fragments arranged by size:
4278 1572 658
ApaLI G'TGCA_C
Cuts at: 4565 5721 4565
Size: 1156 5352
ApoI r'AATT_y
Cuts at: 1493 2485 2494 3817 4043 4195 6304 1493
Size: 992 9 1323 226 152 2109 1697
Fragments arranged by size:
2109 1697 1323 992 226 152 9
AseI AT'TA_AT
Cuts at: 160 1032 2858 5062 160
Size: 872 1826 2204 1606
Fragments arranged by size:
2204 1826 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
AvaI C'yCGr_G
Cuts at: 1870 1943 2580 3833 1870
Size: 73 637 1253 4545
Fragments arranged by size:
4545 1253 637 73
AvrII C'CTAG_G
Cuts at: 3560 3560
Size: 6508
BamHI G'GATC_C
Cuts at: 3838 4205 3838
Size: 367 6141
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2280 3829 5284 618
Size: 325 217 627 493 1549 1455 1842
Fragments arranged by size:
1842 1549 1455 627 493 325 217
BanII G_rGCy'C
Cuts at: 1391 3827 1391
Size: 2436 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5537
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4699 4733 1903
Size: 34 2762 34 3678
Fragments arranged by size:
3678 2762 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4400 5610 4400
Size: 1210 5298
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6508
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5119 136
Size: 107 122 71 631 605 3447 1525
Fragments arranged by size:
3447 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2321 3017 6503 2321
Size: 696 3486 2326
Fragments arranged by size:
3486 2326 696
BlpI GC'TnA_GC
Cuts at: 3925 3925
Size: 6508
Bme1580I G_kGCm'C
Cuts at: 1790 4569 5725 1790
Size: 2779 1156 2573
Fragments arranged by size:
2779 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6508
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2702 4239 5197
Size: 416 53 83 399 24 1285 1537 958
Cuts at: 5197 442
Size: 1753
Fragments arranged by size:
1753 1537 1285 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 2603 5168 1239
Size: 1076 288 2565 2579
Fragments arranged by size:
2579 2565 1076 288
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4633 5498 5796 4633
Size: 865 298 5345
Fragments arranged by size:
5345 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5171 882
Size: 617 3672 2219
Fragments arranged by size:
3672 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4299 493
Size: 711 3095 2702
Fragments arranged by size:
3095 2702 711
BsaBI GATnn'nnATC
Cuts at: 6502 6502
Size: 6508
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4315 4697 5332 275
Size: 53 83 186 936 2782 382 635 1451
Fragments arranged by size:
2782 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4941 5339 5613 5760 6171 4941
Size: 398 274 147 411 5278
Fragments arranged by size:
5278 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2947 2977 1178
Size: 30 165 30 1544 30 4709
Fragments arranged by size:
4709 1544 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2568 1186
Size: 1382 5126
BseYI C'CCAG_C
Cuts at: 5711 6207 5711
Size: 496 6012
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5935
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3958 4719 5747 1540
Size: 275 186 1957 761 1028 2301
Fragments arranged by size:
2301 1957 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4569 4654 5725 4569
Size: 85 1071 5352
Fragments arranged by size:
5352 1071 85
BsmI GAATG_Cn'
Cuts at: 4019 4112 4019
Size: 93 6415
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3827 4569 4654 5725 1391
Size: 399 2037 742 85 1071 2174
Fragments arranged by size:
2174 2037 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6171 6171
Size: 6508
BspHI T'CATG_A
Cuts at: 3060 4397 6127 3060
Size: 1337 1730 3441
Fragments arranged by size:
3441 1730 1337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2151 3601 3863 844
Size: 1307 1450 262 3489
Fragments arranged by size:
3489 1450 1307 262
BsrBI CCG'CTC
Cuts at: 1394 1823 4395 1394
Size: 429 2572 3507
Fragments arranged by size:
3507 2572 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2146 3578 3648 5003 5177 65
Size: 1183 898 1432 70 1355 174 1396
Fragments arranged by size:
1432 1396 1355 1183 898 174 70
BsrFI r'CCGG_y
Cuts at: 1700 1810 5152 5339 1700
Size: 110 3342 187 2869
Fragments arranged by size:
3342 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3226 96
Size: 3130 3378
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6508
BssSI C'ACGA_G
Cuts at: 4568 5580 4568
Size: 1012 5496
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6508
BstBI TT'CG_AA
Cuts at: 5312 5312
Size: 6508
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6508
BstYI r'GATC_y
Cuts at: 1972 2321 3017 3047 3838 4205 4590 4607 6059
Size: 349 696 30 791 367 385 17 1452
Cuts at: 6059 6145 6157 6503 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 791 696 385 367 349 346
86 30 17 12
Bsu36I CC'TnA_GG
Cuts at: 3674 3674
Size: 6508
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5017
Fragments arranged by size:
5017 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5769
Fragments arranged by size:
5769 683 56
BtsI GCAGTG_nn'
Cuts at: 4024 4818 4838 4024
Size: 794 20 5694
Fragments arranged by size:
5694 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6508
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2258 2293 3160 3195 529
Size: 35 1694 35 867 35 3842
Fragments arranged by size:
3842 1694 867 35 35 35
DraI TTT'AAA
Cuts at: 2236 3812 2236
Size: 1576 4932
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5515 809
Size: 507 4199 1802
Fragments arranged by size:
4199 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3955 4844 6473
Size: 54 1425 50 275 18 2125 889 1629
Cuts at: 6473 8
Size: 43
Fragments arranged by size:
2125 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3955 1537
Size: 275 2143 4090
Fragments arranged by size:
4090 2143 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 2423 2799 3629 4438 1353
Size: 668 402 376 830 809 3423
Fragments arranged by size:
3423 830 809 668 402 376
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5095 5469 5615 387
Size: 1064 138 3506 374 146 1280
Fragments arranged by size:
3506 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 2603 2913 2974 2985 3228 3653
Size: 393 683 288 310 61 11 243 425
Cuts at: 3653 4571 5168 5955 6223 1239
Size: 918 597 787 268 1524
Fragments arranged by size:
1524 918 787 683 597 425 393 310
288 268 243 61 11
EcoRI G'AATT_C
Cuts at: 2485 3817 4195 2485
Size: 1332 378 4798
Fragments arranged by size:
4798 1332 378
FspI TGC'GCA
Cuts at: 5014 5014
Size: 6508
HaeII r_GCGC'y
Cuts at: 1678 5655 1678
Size: 3977 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2306 2338 2947 2979 5149
Size: 32 135 32 734 32 609 32 2170
Cuts at: 5149 5181 5223 5255 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2170 734 609 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3055 3852 4098 669
Size: 988 281 1117 797 246 3079
Fragments arranged by size:
3079 1117 988 797 281 246
HindIII A'AGCT_T
Cuts at: 748 3340 748
Size: 2592 3916
HpaI GTT'AAC
Cuts at: 4098 4098
Size: 6508
KpnI G_GTAC'C
Cuts at: 2284 3833 2284
Size: 1549 4959
MfeI C'AATT_G
Cuts at: 2526 3525 4107 2526
Size: 999 582 4927
Fragments arranged by size:
4927 999 582
MluI A'CGCG_T
Cuts at: 6384 6384
Size: 6508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3225 3251 5622 5806 1305
Size: 1920 26 2371 184 2007
Fragments arranged by size:
2371 2007 1920 184 26
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4686
Fragments arranged by size:
4686 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4466 4825 4984 518
Size: 648 3300 359 159 2042
Fragments arranged by size:
3300 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2558 3924 4601 5749 5994 1606
Size: 952 1366 677 1148 245 2120
Fragments arranged by size:
2120 1366 1148 952 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5017
Fragments arranged by size:
5017 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6508
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6508
NotI GC'GGCC_GC
Cuts at: 3955 3955
Size: 6508
NspI r_CATG'y
Cuts at: 1132 1229 3227 3866 5411 1132
Size: 97 1998 639 1545 2229
Fragments arranged by size:
2229 1998 1545 639 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6508
PciI A'CATG_T
Cuts at: 5407 5407
Size: 6508
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6508
PmeI GTTT'AAAC
Cuts at: 3812 3812
Size: 6508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4577 4609 6090 6122 4577
Size: 32 1481 32 4963
Fragments arranged by size:
4963 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3141 1613
Size: 1528 4980
PsiI TTA'TAA
Cuts at: 4078 4078
Size: 6508
PstI C_TGCA'G
Cuts at: 830 3598 3860 830
Size: 2768 262 3478
Fragments arranged by size:
3478 2768 262
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6508
PvuII CAG'CTG
Cuts at: 2558 2558
Size: 6508
SalI G'TCGA_C
Cuts at: 1936 3850 1936
Size: 1914 4594
SbfI CC_TGCA'GG
Cuts at: 3860 3860
Size: 6508
ScaI AGT'ACT
Cuts at: 3767 4756 6468 3767
Size: 989 1712 3807
Fragments arranged by size:
3807 1712 989
SfcI C'TryA_G
Cuts at: 826 1045 3594 3856 4991 5672 5863 826
Size: 219 2549 262 1135 681 191 1471
Fragments arranged by size:
2549 1471 1135 681 262 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6508
SmaI CCC'GGG
Cuts at: 1872 3835 1872
Size: 1963 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4612 5513 5775 820
Size: 197 926 2669 901 262 1553
Fragments arranged by size:
2669 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4299 493
Size: 3806 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6508
SphI G_CATG'C
Cuts at: 1132 3866 1132
Size: 2734 3774
SspI AAT'ATT
Cuts at: 5 52 4432 5
Size: 47 4380 2081
Fragments arranged by size:
4380 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6508
StyI C'CwwG_G
Cuts at: 513 1065 2004 2021 3560 3947 513
Size: 552 939 17 1539 387 3074
Fragments arranged by size:
3074 1539 939 552 387 17
SwaI ATTT'AAAT
Cuts at: 2236 2236
Size: 6508
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2717 3462 4535 4552 4705 4890 5335
Size: 1255 1137 745 1073 17 153 185 445
Cuts at: 5335 325
Size: 1498
Fragments arranged by size:
1498 1255 1137 1073 745 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3226 3765 4754
Size: 274 80 33 51 1443 1249 539 989
Cuts at: 4754 6466 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1249 989 539 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2965 2997 6468 6500 2965
Size: 32 3471 32 2973
Fragments arranged by size:
3471 2973 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6508
XbaI T'CTAG_A
Cuts at: 2489 3844 2489
Size: 1355 5153
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6508
XmaI C'CCGG_G
Cuts at: 1870 3833 1870
Size: 1963 4545
XmnI GAAnn'nnTTC
Cuts at: 3683 4637 3683
Size: 954 5554
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4316 276
Size: 53 83 186 936 2782 2468
Fragments arranged by size:
2782 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI XcmI