Restriction map for
pFN21AE0356
(Circular) (Six-base) MAPSORT of: pFN21AE0356.seq Check: 6406 from: 1 to: 6220
pFN21AE0356 6220 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4030 278
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3563 1937
Size: 1626 4594
Acc65I G'GTAC_C
Cuts at: 3541 3541
Size: 6220
AclI AA'CG_TT
Cuts at: 1563 2933 4347 4720 1563
Size: 1370 1414 373 3063
Fragments arranged by size:
3063 1414 1370 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2006 2260 2575 2925 2943 4283 5667 5935
Size: 374 254 315 350 18 1340 1384 268
Cuts at: 5935 1632
Size: 1917
Fragments arranged by size:
1917 1384 1340 374 350 315 268 254
18
AflII C'TTAA_G
Cuts at: 820 1017 2807 820
Size: 197 1790 4233
Fragments arranged by size:
4233 1790 197
AflIII A'CryG_T
Cuts at: 1203 2548 5119 6096 1203
Size: 1345 2571 977 1327
Fragments arranged by size:
2571 1345 1327 977
AgeI A'CCGG_T
Cuts at: 3136 5051 3136
Size: 1915 4305
AhdI GACnn_n'nnGTC
Cuts at: 4949 4949
Size: 6220
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2090 3390 1184
Size: 906 1300 4014
Fragments arranged by size:
4014 1300 906
AlwNI CAG_nnn'CTG
Cuts at: 3963 5535 3963
Size: 1572 4648
ApaLI G'TGCA_C
Cuts at: 4277 5433 4277
Size: 1156 5064
ApoI r'AATT_y
Cuts at: 1493 2717 2747 3301 3529 3755 3907 6016 1493
Size: 1224 30 554 228 226 152 2109 1697
Fragments arranged by size:
2109 1697 1224 554 228 226 152 30
AseI AT'TA_AT
Cuts at: 160 1032 4774 160
Size: 872 3742 1606
Fragments arranged by size:
3742 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 3545 1870
Size: 73 1602 4545
Fragments arranged by size:
4545 1602 73
AvrII C'CTAG_G
Cuts at: 3046 3046
Size: 6220
BamHI G'GATC_C
Cuts at: 2979 3550 3917 2979
Size: 571 367 5282
Fragments arranged by size:
5282 571 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3541 4996 618
Size: 325 217 627 1754 1455 1842
Fragments arranged by size:
1842 1754 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2131 2541 3235 3539 1391
Size: 622 118 410 694 304 4072
Fragments arranged by size:
4072 694 622 410 304 118
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3051 3267 928
Size: 971 1152 216 3881
Fragments arranged by size:
3881 1152 971 216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4411 4445 1903
Size: 34 2474 34 3678
Fragments arranged by size:
3678 2474 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4112 5322 4112
Size: 1210 5010
BclI T'GATC_A
Cuts at: 1548 2121 1548
Size: 573 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2017 2601 4831
Size: 107 122 71 631 605 345 584 2230
Cuts at: 4831 136
Size: 1525
Fragments arranged by size:
2230 1525 631 605 584 345 122 107
71
BglII A'GATC_T
Cuts at: 2911 6215 2911
Size: 3304 2916
BlpI GC'TnA_GC
Cuts at: 3637 3637
Size: 6220
Bme1580I G_kGCm'C
Cuts at: 1790 4281 5437 1790
Size: 2491 1156 2573
Fragments arranged by size:
2573 2491 1156
BmgBI CAC'GTC
Cuts at: 1318 2531 1318
Size: 1213 5007
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2183 3951 4909
Size: 416 53 83 399 24 766 1768 958
Cuts at: 4909 442
Size: 1753
Fragments arranged by size:
1768 1753 958 766 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2555 2900 3277 3417 4880 1239
Size: 1316 345 377 140 1463 2579
Fragments arranged by size:
2579 1463 1316 377 345 140
Bpu10I CC'TnA_GC
Cuts at: 2657 3006 3080 2657
Size: 349 74 5797
Fragments arranged by size:
5797 349 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2712 3304 3427 3461 4345 5210 5508 2712
Size: 592 123 34 884 865 298 3424
Fragments arranged by size:
3424 884 865 592 298 123 34
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4883 882
Size: 617 3384 2219
Fragments arranged by size:
3384 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4011 493
Size: 711 2807 2702
Fragments arranged by size:
2807 2702 711
BsaBI GATnn'nnATC
Cuts at: 3107 6214 3107
Size: 3107 3113
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4027 4409 5044 275
Size: 53 83 186 936 2494 382 635 1451
Fragments arranged by size:
2494 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2793 3136 4653 5051 5325 5472 5883 2793
Size: 343 1517 398 274 147 411 3130
Fragments arranged by size:
3130 1517 411 398 343 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5995
Fragments arranged by size:
5995 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2122 2125 1186
Size: 865 71 3 5281
Fragments arranged by size:
5281 865 71 3
BseYI C'CCAG_C
Cuts at: 2847 3453 5423 5919 2847
Size: 606 1970 496 3148
Fragments arranged by size:
3148 1970 606 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2117 2130 1163
Size: 573 381 13 5253
Fragments arranged by size:
5253 573 381 13
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3670 4431 5459 1540
Size: 275 186 1669 761 1028 2301
Fragments arranged by size:
2301 1669 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2541 4281 4366 5437 2541
Size: 1740 85 1071 3324
Fragments arranged by size:
3324 1740 1071 85
BsmI GAATG_Cn'
Cuts at: 2445 3122 3731 3824 2445
Size: 677 609 93 4841
Fragments arranged by size:
4841 677 609 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2131 2541 3235 3539 4281 4366
Size: 399 223 118 410 694 304 742 85
Cuts at: 4366 5437 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 694 410 399 304 223
118 85
BspEI T'CCGG_A
Cuts at: 5883 5883
Size: 6220
BspHI T'CATG_A
Cuts at: 2577 4109 5839 2577
Size: 1532 1730 2958
Fragments arranged by size:
2958 1730 1532
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3208 3575 844
Size: 2364 367 3489
Fragments arranged by size:
3489 2364 367
BsrBI CCG'CTC
Cuts at: 1394 1823 4107 1394
Size: 429 2284 3507
Fragments arranged by size:
3507 2284 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2514 4715 4889 65
Size: 1183 1266 2201 174 1396
Fragments arranged by size:
2201 1396 1266 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3136 4864 5051 1700
Size: 110 1326 1728 187 2869
Fragments arranged by size:
2869 1728 1326 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6220
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6220
BssSI C'ACGA_G
Cuts at: 4280 5292 4280
Size: 1012 5208
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2153 794
Size: 1359 4861
BstBI TT'CG_AA
Cuts at: 5024 5024
Size: 6220
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6220
BstYI r'GATC_y
Cuts at: 1972 2323 2911 2979 3550 3917 4302 4319 5771
Size: 351 588 68 571 367 385 17 1452
Cuts at: 5771 5857 5869 6215 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 588 571 385 367 351 346
86 68 17 12
Bsu36I CC'TnA_GG
Cuts at: 3267 3267
Size: 6220
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4729
Fragments arranged by size:
4729 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5481
Fragments arranged by size:
5481 683 56
BtsI GCAGTG_nn'
Cuts at: 2652 3405 3736 4530 4550 2652
Size: 753 331 794 20 4322
Fragments arranged by size:
4322 794 753 331 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6185
DraI TTT'AAA
Cuts at: 3524 3524
Size: 6220
DraIII CAC_nnn'GTG
Cuts at: 3105 3105
Size: 6220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3269 5227 809
Size: 507 1953 1958 1802
Fragments arranged by size:
1958 1953 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2170 3667 4556
Size: 54 1425 50 275 18 340 1497 889
Cuts at: 4556 6185 8
Size: 1629 43
Fragments arranged by size:
1629 1497 1425 889 340 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3667 1537
Size: 275 1855 4090
Fragments arranged by size:
4090 1855 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3240 4150 1353
Size: 1887 910 3423
Fragments arranged by size:
3423 1887 910
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3113 4807 5181 5327 387
Size: 1064 138 1524 1694 374 146 1280
Fragments arranged by size:
1694 1524 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2539 2539
Size: 6220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2006 2260 2555 2575 2900 2925 2943
Size: 393 374 254 295 20 325 25 18
Cuts at: 2943 3277 3417 4283 4880 5667 5935 1239
Size: 334 140 866 597 787 268 1524
Fragments arranged by size:
1524 866 787 597 393 374 334 325
295 268 254 140 25 20 18
EcoRI G'AATT_C
Cuts at: 3529 3907 3529
Size: 378 5842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 6220
FspI TGC'GCA
Cuts at: 4726 4726
Size: 6220
HaeII r_GCGC'y
Cuts at: 1678 2848 5367 1678
Size: 1170 2519 2531
Fragments arranged by size:
2531 2519 1170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2106 2138 2945 2977 3018
Size: 32 135 32 534 32 807 32 41
Cuts at: 3018 3050 3176 3208 4861 4893 4935 4967 1373
Size: 32 126 32 1653 32 42 32 2626
Fragments arranged by size:
2626 1653 807 534 135 126 42 41
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3564 3810 669
Size: 988 281 1626 246 3079
Fragments arranged by size:
3079 1626 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6220
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KpnI G_GTAC'C
Cuts at: 3545 3545
Size: 6220
MfeI C'AATT_G
Cuts at: 3819 3819
Size: 6220
MluI A'CGCG_T
Cuts at: 6096 6096
Size: 6220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2236 2595 2694 5334 5518 1305
Size: 931 359 99 2640 184 2007
Fragments arranged by size:
2640 2007 931 359 184 99
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2172 10
Size: 54 1425 343 340 4058
Fragments arranged by size:
4058 1425 343 340 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2465 2468 3101 3321 4178 4537 4696
Size: 648 1299 3 633 220 857 359 159
Cuts at: 4696 518
Size: 2042
Fragments arranged by size:
2042 1299 857 648 633 359 220 159
3
MspA1I CmG'CkG
Cuts at: 1606 3344 3636 4313 5461 5706 1606
Size: 1738 292 677 1148 245 2120
Fragments arranged by size:
2120 1738 1148 677 292 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4729
Fragments arranged by size:
4729 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6220
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6220
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 2071 2552 3578 5123 1132
Size: 97 842 481 1026 1545 2229
Fragments arranged by size:
2229 1545 1026 842 481 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6220
PciI A'CATG_T
Cuts at: 2548 5119 2548
Size: 2571 3649
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6220
PfoI T'CCnGG_A
Cuts at: 2787 2975 2787
Size: 188 6032
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4289 4321 5802 5834 4289
Size: 32 1481 32 4675
Fragments arranged by size:
4675 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6220
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 6220
PstI C_TGCA'G
Cuts at: 830 3205 3572 830
Size: 2375 367 3478
Fragments arranged by size:
3478 2375 367
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
PvuII CAG'CTG
Cuts at: 3344 3344
Size: 6220
SacI G_AGCT'C
Cuts at: 2541 2541
Size: 6220
SalI G'TCGA_C
Cuts at: 1936 3562 1936
Size: 1626 4594
SapI GCTCTTCn'nnn_
Cuts at: 3240 3240
Size: 6220
SbfI CC_TGCA'GG
Cuts at: 3205 3572 3205
Size: 367 5853
ScaI AGT'ACT
Cuts at: 4468 6180 4468
Size: 1712 4508
SexAI A'CCwGG_T
Cuts at: 2706 2706
Size: 6220
SfcI C'TryA_G
Cuts at: 826 1045 3201 3568 4703 5384 5575 826
Size: 219 2156 367 1135 681 191 1471
Fragments arranged by size:
2156 1471 1135 681 367 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6220
SmaI CCC'GGG
Cuts at: 1872 3547 1872
Size: 1675 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2727 2807 3283 3442 3476 4324
Size: 197 926 784 80 476 159 34 848
Cuts at: 4324 5225 5487 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 901 848 784 476 262 197
159 80 34
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 3578 1132
Size: 2446 3774
SspI AAT'ATT
Cuts at: 5 52 2457 3226 4144 5
Size: 47 2405 769 918 2081
Fragments arranged by size:
2405 2081 918 769 47
StuI AGG'CCT
Cuts at: 1517 3448 1517
Size: 1931 4289
StyI C'CwwG_G
Cuts at: 513 1065 2004 3046 3659 513
Size: 552 939 1042 613 3074
Fragments arranged by size:
3074 1042 939 613 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4247 4264 4417 4602 5047 325
Size: 1255 2667 17 153 185 445 1498
Fragments arranged by size:
2667 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4466 6178 96
Size: 274 80 33 51 1443 2489 1712 138
Fragments arranged by size:
2489 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3198 3230 3483 3515 6180 6212 3198
Size: 32 253 32 2665 32 3206
Fragments arranged by size:
3206 2665 253 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6220
XbaI T'CTAG_A
Cuts at: 3556 3556
Size: 6220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2165 2165
Size: 6220
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6220
XmaI C'CCGG_G
Cuts at: 1870 3545 1870
Size: 1675 4545
XmnI GAAnn'nnTTC
Cuts at: 2456 2933 4349 2456
Size: 477 1416 4327
Fragments arranged by size:
4327 1416 477
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4028 276
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI