Restriction map for
pFN21AE0354
(Circular) (Six-base) MAPSORT of: pFN21AE0354.seq Check: 8623 from: 1 to: 6862
pFN21AE0354 6862 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4076 4076
Size: 6862
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2460 4672 278
Size: 53 83 186 936 924 2212 2468
Fragments arranged by size:
2468 2212 936 924 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4205 1937
Size: 2268 4594
Acc65I G'GTAC_C
Cuts at: 4183 4183
Size: 6862
AclI AA'CG_TT
Cuts at: 1563 4989 5362 1563
Size: 3426 373 3063
Fragments arranged by size:
3426 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4925 6309 6577 1632
Size: 3293 1384 268 1917
Fragments arranged by size:
3293 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6665
AflIII A'CryG_T
Cuts at: 1203 2292 2781 3357 5761 6738 1203
Size: 1089 489 576 2404 977 1327
Fragments arranged by size:
2404 1327 1089 977 576 489
AgeI A'CCGG_T
Cuts at: 5693 5693
Size: 6862
AhdI GACnn_n'nnGTC
Cuts at: 3619 5591 3619
Size: 1972 4890
AleI CACnn'nnGTG
Cuts at: 1166 2291 2828 3584 1166
Size: 1125 537 756 4444
Fragments arranged by size:
4444 1125 756 537
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6862
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2948 2980 2948
Size: 32 6830
AlwNI CAG_nnn'CTG
Cuts at: 3679 3792 3805 4096 4605 6177 3679
Size: 113 13 291 509 1572 4364
Fragments arranged by size:
4364 1572 509 291 113 13
ApaI G_GGCC'C
Cuts at: 2539 3044 3213 3248 3635 3757 2539
Size: 505 169 35 387 122 5644
Fragments arranged by size:
5644 505 387 169 122 35
ApaLI G'TGCA_C
Cuts at: 3274 4919 6075 3274
Size: 1645 1156 4061
Fragments arranged by size:
4061 1645 1156
ApoI r'AATT_y
Cuts at: 1493 4171 4397 4549 6658 1493
Size: 2678 226 152 2109 1697
Fragments arranged by size:
2678 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5416 160
Size: 872 4384 1606
Fragments arranged by size:
4384 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6862
AvaI C'yCGr_G
Cuts at: 1870 1943 4187 1870
Size: 73 2244 4545
Fragments arranged by size:
4545 2244 73
BamHI G'GATC_C
Cuts at: 2133 4192 4559 2133
Size: 2059 367 4436
Fragments arranged by size:
4436 2059 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2070 2720 3312 3511 4183
Size: 325 217 627 283 650 592 199 672
Cuts at: 4183 5638 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 672 650 627 592 325 283
217 199
BanII G_rGCy'C
Cuts at: 1391 2539 3029 3044 3213 3248 3635 3757 3786
Size: 1148 490 15 169 35 387 122 29
Cuts at: 3786 4181 1391
Size: 395 4072
Fragments arranged by size:
4072 1148 490 395 387 169 122 35
29 15
BbeI G_GCGC'C
Cuts at: 2074 2724 3316 2074
Size: 650 592 5620
Fragments arranged by size:
5620 650 592
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2280 2410 3553 928
Size: 971 381 130 1143 4237
Fragments arranged by size:
4237 1143 971 381 130
BbvCI CC'TCA_GC
Cuts at: 3452 3938 3452
Size: 486 6376
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2066 2100 5053 5087 1903
Size: 34 129 34 2953 34 3678
Fragments arranged by size:
3678 2953 129 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2364 4754 5964 2364
Size: 2390 1210 3262
Fragments arranged by size:
3262 2390 1210
BclI T'GATC_A
Cuts at: 1548 3603 1548
Size: 2055 4807
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5473 136
Size: 107 122 71 631 605 3801 1525
Fragments arranged by size:
3801 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3012 6857 3012
Size: 3845 3017
BlpI GC'TnA_GC
Cuts at: 2253 3023 4279 2253
Size: 770 1256 4836
Fragments arranged by size:
4836 1256 770
Bme1580I G_kGCm'C
Cuts at: 1790 2539 2801 3044 3213 3241 3248 3278 3635
Size: 749 262 243 169 28 7 30 357
Cuts at: 3635 3757 4923 6079 1790
Size: 122 1166 1156 2573
Fragments arranged by size:
2573 1166 1156 749 357 262 243 169
122 30 28 7
BmgBI CAC'GTC
Cuts at: 1318 2293 3070 3469 3601 1318
Size: 975 777 399 132 4579
Fragments arranged by size:
4579 975 777 399 132
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4593 5551 442
Size: 416 53 83 399 24 3176 958 1753
Fragments arranged by size:
3176 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6862
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3928 3928
Size: 6862
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2567 2905 3194 3611 3668 3701 3705 3761
Size: 1328 338 289 417 57 33 4 56
Cuts at: 3761 3893 5522 1239
Size: 132 1629 2579
Fragments arranged by size:
2579 1629 1328 417 338 289 132 57
56 33 4
Bpu10I CC'TnA_GC
Cuts at: 3452 3785 3938 3452
Size: 333 153 6376
Fragments arranged by size:
6376 333 153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4987 5852 6150 4987
Size: 865 298 5699
Fragments arranged by size:
5699 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5525 882
Size: 617 4026 2219
Fragments arranged by size:
4026 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3358 4653 493
Size: 711 2154 1295 2702
Fragments arranged by size:
2702 2154 1295 711
BsaBI GATnn'nnATC
Cuts at: 6856 6856
Size: 6862
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2071 2415 2457 2721
Size: 53 83 186 936 538 344 42 264
Cuts at: 2721 3313 4669 5051 5686 275
Size: 592 1356 382 635 1451
Fragments arranged by size:
1451 1356 936 635 592 538 382 344
264 186 83 53 42
BsaWI w'CCGG_w
Cuts at: 5295 5693 5967 6114 6525 5295
Size: 398 274 147 411 5632
Fragments arranged by size:
5632 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2296 2326 3641 3653 3671
Size: 30 165 30 893 30 1315 12 18
Cuts at: 3671 3683 1178
Size: 12 4357
Fragments arranged by size:
4357 1315 893 165 30 30 30 18
12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2981 3200 3389 3704 1186
Size: 877 918 219 189 315 4344
Fragments arranged by size:
4344 918 877 315 219 189
BseYI C'CCAG_C
Cuts at: 2054 2191 2904 3037 3044 3090 3222 3247 3382
Size: 137 713 133 7 46 132 25 135
Cuts at: 3382 3652 6065 6561 2054
Size: 270 2413 496 2355
Fragments arranged by size:
2413 2355 713 496 270 137 135 133
132 46 25 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3029 1163
Size: 573 1293 4996
Fragments arranged by size:
4996 1293 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3442 3585 4312 5073 6101 1540
Size: 275 186 1441 143 727 761 1028 2301
Fragments arranged by size:
2301 1441 1028 761 727 275 186 143
BsiHKAI G_wGCw'C
Cuts at: 2649 3278 3521 4923 5008 6079 2649
Size: 629 243 1402 85 1071 3432
Fragments arranged by size:
3432 1402 1071 629 243 85
BsmI GAATG_Cn'
Cuts at: 3958 4373 4466 3958
Size: 415 93 6354
Fragments arranged by size:
6354 415 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2539 2649 2801 3029 3044 3213 3241
Size: 399 749 110 152 228 15 169 28
Cuts at: 3241 3248 3278 3521 3635 3757 3786 4181 4923
Size: 7 30 243 114 122 29 395 742
Cuts at: 4923 5008 6079 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 749 742 399 395 243 228
169 152 122 114 110 85 30 29
28 15 7
BspEI T'CCGG_A
Cuts at: 6525 6525
Size: 6862
BspHI T'CATG_A
Cuts at: 4751 6481 4751
Size: 1730 5132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3624 3648 4076 4217 844
Size: 2780 24 428 141 3489
Fragments arranged by size:
3489 2780 428 141 24
BsrBI CCG'CTC
Cuts at: 1394 1823 3444 4039 4749 1394
Size: 429 1621 595 710 3507
Fragments arranged by size:
3507 1621 710 595 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3732 5357 5531 65
Size: 1183 2484 1625 174 1396
Fragments arranged by size:
2484 1625 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3306 3530 5506 5693 1700
Size: 110 1496 224 1976 187 2869
Fragments arranged by size:
2869 1976 1496 224 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6862
BssHII G'CGCG_C
Cuts at: 1925 3433 1925
Size: 1508 5354
BssSI C'ACGA_G
Cuts at: 2039 2714 4922 5934 2039
Size: 675 2208 1012 2967
Fragments arranged by size:
2967 2208 1012 675
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3805 3953 4077 794
Size: 3011 148 124 3579
Fragments arranged by size:
3579 3011 148 124
BstBI TT'CG_AA
Cuts at: 5666 5666
Size: 6862
BstEII G'GTnAC_C
Cuts at: 1184 2026 1184
Size: 842 6020
BstYI r'GATC_y
Cuts at: 1972 2133 3012 4192 4559 4944 4961 6413 6499
Size: 161 879 1180 367 385 17 1452 86
Cuts at: 6499 6511 6857 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1180 879 385 367 346 161
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2569 2855 3585 513
Size: 552 939 565 286 730 3790
Fragments arranged by size:
3790 939 730 565 552 286
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2946 487
Size: 683 56 1720 4403
Fragments arranged by size:
4403 1720 683 56
BtsI GCAGTG_nn'
Cuts at: 2023 2724 3815 4081 4378 5172 5192 2023
Size: 701 1091 266 297 794 20 3693
Fragments arranged by size:
3693 1091 794 701 297 266 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6862
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6827
DraI TTT'AAA
Cuts at: 4166 4166
Size: 6862
DraIII CAC_nnn'GTG
Cuts at: 2809 2925 2809
Size: 116 6746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5869 809
Size: 507 4553 1802
Fragments arranged by size:
4553 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2443 2596 2700
Size: 54 1425 50 275 18 613 153 104
Cuts at: 2700 3582 4149 4309 5198 6827 8
Size: 882 567 160 889 1629 43
Fragments arranged by size:
1629 1425 889 882 613 567 275 160
153 104 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3582 4309 1537
Size: 275 1770 727 4090
Fragments arranged by size:
4090 1770 727 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2754 4792 1353
Size: 1401 2038 3423
Fragments arranged by size:
3423 2038 1401
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5449 5823 5969 387
Size: 1064 138 3860 374 146 1280
Fragments arranged by size:
3860 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2567 2905 3194 3611 3668 3701 3705
Size: 393 935 338 289 417 57 33 4
Cuts at: 3705 3761 3893 4925 5522 6309 6577 1239
Size: 56 132 1032 597 787 268 1524
Fragments arranged by size:
1524 1032 935 787 597 417 393 338
289 268 132 57 56 33 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2549 2702 2771 2549
Size: 153 69 6640
Fragments arranged by size:
6640 153 69
EcoO109I rG'GnC_Cy
Cuts at: 2235 2536 2764 2880 3041 3209 3210 3244 3328
Size: 301 228 116 161 168 1 34 84
Cuts at: 3328 3631 3632 3753 3789 2235
Size: 303 1 121 36 5308
Fragments arranged by size:
5308 303 301 228 168 161 121 116
84 36 34 1 1
EcoRI G'AATT_C
Cuts at: 4171 4549 4171
Size: 378 6484
FspI TGC'GCA
Cuts at: 5368 5368
Size: 6862
HaeII r_GCGC'y
Cuts at: 1678 2074 2724 3316 3459 3574 6009 1678
Size: 396 650 592 143 115 2435 2531
Fragments arranged by size:
2531 2435 650 592 396 143 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3136 3168 3605 3637 5503
Size: 32 135 32 1564 32 437 32 1866
Cuts at: 5503 5535 5577 5609 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1866 1564 437 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4206 4452 669
Size: 988 281 2268 246 3079
Fragments arranged by size:
3079 2268 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6862
HpaI GTT'AAC
Cuts at: 4452 4452
Size: 6862
KasI G'GCGC_C
Cuts at: 2070 2720 3312 2070
Size: 650 592 5620
Fragments arranged by size:
5620 650 592
KpnI G_GTAC'C
Cuts at: 4187 4187
Size: 6862
MfeI C'AATT_G
Cuts at: 4461 4461
Size: 6862
MluI A'CGCG_T
Cuts at: 2781 6738 2781
Size: 3957 2905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2041 3639 5976 6160 1305
Size: 736 1598 2337 184 2007
Fragments arranged by size:
2337 2007 1598 736 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2702 4151 10
Size: 54 1425 343 870 1449 2721
Fragments arranged by size:
2721 1449 1425 870 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2291 2604 2828 3584 4098 4820 5179
Size: 648 1125 313 224 756 514 722 359
Cuts at: 5179 5338 518
Size: 159 2042
Fragments arranged by size:
2042 1125 756 722 648 514 359 313
224 159
MspA1I CmG'CkG
Cuts at: 1606 2191 2448 2521 2895 3090 3480 3667 3802
Size: 585 257 73 374 195 390 187 135
Cuts at: 3802 3982 4278 4955 6103 6348 1606
Size: 180 296 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 585 390 374 296 257
245 195 187 180 135 73
NaeI GCC'GGC
Cuts at: 3532 3532
Size: 6862
NarI GG'CG_CC
Cuts at: 2071 2721 3313 2071
Size: 650 592 5620
Fragments arranged by size:
5620 650 592
NcoI C'CATG_G
Cuts at: 513 1065 2004 2855 513
Size: 552 939 851 4520
Fragments arranged by size:
4520 939 851 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6862
NgoMIV G'CCGG_C
Cuts at: 3530 3530
Size: 6862
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6862
NotI GC'GGCC_GC
Cuts at: 4309 4309
Size: 6862
NspI r_CATG'y
Cuts at: 1132 1229 4220 5765 1132
Size: 97 2991 1545 2229
Fragments arranged by size:
2991 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3213 1348
Size: 1865 4997
PciI A'CATG_T
Cuts at: 5761 5761
Size: 6862
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2041 2698 3505 4096 1108
Size: 933 657 807 591 3874
Fragments arranged by size:
3874 933 807 657 591
PfoI T'CCnGG_A
Cuts at: 2383 2544 3870 2383
Size: 161 1326 5375
Fragments arranged by size:
5375 1326 161
PmeI GTTT'AAAC
Cuts at: 4166 4166
Size: 6862
PmlI CAC'GTG
Cuts at: 3358 3358
Size: 6862
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2246 2278 4931 4963 6444 6476 2246
Size: 32 2653 32 1481 32 2632
Fragments arranged by size:
2653 2632 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3789 3789
Size: 6862
PshAI GACnn'nnGTC
Cuts at: 1613 2295 1613
Size: 682 6180
PsiI TTA'TAA
Cuts at: 4432 4432
Size: 6862
PspOMI G'GGCC_C
Cuts at: 2535 3040 3209 3244 3631 3753 2535
Size: 505 169 35 387 122 5644
Fragments arranged by size:
5644 505 387 169 122 35
PstI C_TGCA'G
Cuts at: 830 2079 3235 3621 3810 4088 4214 830
Size: 1249 1156 386 189 278 126 3478
Fragments arranged by size:
3478 1249 1156 386 278 189 126
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6862
PvuII CAG'CTG
Cuts at: 2191 2895 3667 3802 3982 2191
Size: 704 772 135 180 5071
Fragments arranged by size:
5071 772 704 180 135
SalI G'TCGA_C
Cuts at: 1936 4204 1936
Size: 2268 4594
SbfI CC_TGCA'GG
Cuts at: 4214 4214
Size: 6862
ScaI AGT'ACT
Cuts at: 5110 6822 5110
Size: 1712 5150
SexAI A'CCwGG_T
Cuts at: 3504 3504
Size: 6862
SfcI C'TryA_G
Cuts at: 826 1045 2075 3231 3617 3806 4084 4210 5345
Size: 219 1030 1156 386 189 278 126 1135
Cuts at: 5345 6026 6217 826
Size: 681 191 1471
Fragments arranged by size:
1471 1156 1135 1030 681 386 278 219
191 189 126
SfoI GGC'GCC
Cuts at: 2072 2722 3314 2072
Size: 650 592 5620
Fragments arranged by size:
5620 650 592
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6862
SgrAI Cr'CCGG_yG
Cuts at: 1700 3530 1700
Size: 1830 5032
SmaI CCC'GGG
Cuts at: 1872 4189 1872
Size: 2317 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4966 5867 6129 820
Size: 197 926 3023 901 262 1553
Fragments arranged by size:
3023 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4653 493
Size: 4160 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6862
SphI G_CATG'C
Cuts at: 1132 4220 1132
Size: 3088 3774
SspI AAT'ATT
Cuts at: 5 52 4786 5
Size: 47 4734 2081
Fragments arranged by size:
4734 2081 47
StuI AGG'CCT
Cuts at: 1517 2555 3722 1517
Size: 1038 1167 4657
Fragments arranged by size:
4657 1167 1038
StyI C'CwwG_G
Cuts at: 513 1065 2004 2176 2703 2826 2855 3082 3324
Size: 552 939 172 527 123 29 227 242
Cuts at: 3324 4301 513
Size: 977 3074
Fragments arranged by size:
3074 977 939 552 527 242 227 172
123 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3082 4889 4906 5059 5244 5689 325
Size: 1255 1502 1807 17 153 185 445 1498
Fragments arranged by size:
1807 1502 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5108 6820 96
Size: 274 80 33 51 1443 3131 1712 138
Fragments arranged by size:
3131 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2771 2803 6822 6854 2771
Size: 32 4019 32 2779
Fragments arranged by size:
4019 2779 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6862
XbaI T'CTAG_A
Cuts at: 4198 4198
Size: 6862
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 4097 2038
Size: 2059 4803
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6862
XmaI C'CCGG_G
Cuts at: 1870 4187 1870
Size: 2317 4545
XmnI GAAnn'nnTTC
Cuts at: 2528 4991 2528
Size: 2463 4399
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2458 4670 276
Size: 53 83 186 936 924 2212 2468
Fragments arranged by size:
2468 2212 936 924 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI
BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PspXI PsrI RsrII SacI SacII SanDI
SapI SfiI SrfI SwaI