Restriction map for
pFN21AE0353
(Circular) (Six-base) MAPSORT of: pFN21AE0353.seq Check: 7630 from: 1 to: 6334
pFN21AE0353 6334 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4144 278
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2943 3677 1937
Size: 1006 734 4594
Fragments arranged by size:
4594 1006 734
Acc65I G'GTAC_C
Cuts at: 3655 3655
Size: 6334
AclI AA'CG_TT
Cuts at: 1563 4461 4834 1563
Size: 2898 373 3063
Fragments arranged by size:
3063 2898 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3402 4397 5781 6049 1632
Size: 1770 995 1384 268 1917
Fragments arranged by size:
1917 1770 1384 995 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6137
AflIII A'CryG_T
Cuts at: 1203 5233 6210 1203
Size: 4030 977 1327
Fragments arranged by size:
4030 1327 977
AgeI A'CCGG_T
Cuts at: 5165 5165
Size: 6334
AhdI GACnn_n'nnGTC
Cuts at: 5063 5063
Size: 6334
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6334
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2179 2673 1184
Size: 995 494 4845
Fragments arranged by size:
4845 995 494
AlwNI CAG_nnn'CTG
Cuts at: 2235 3049 3425 4077 5649 2235
Size: 814 376 652 1572 2920
Fragments arranged by size:
2920 1572 814 652 376
ApaLI G'TGCA_C
Cuts at: 4391 5547 4391
Size: 1156 5178
ApoI r'AATT_y
Cuts at: 1493 3623 3643 3869 4021 6130 1493
Size: 2130 20 226 152 2109 1697
Fragments arranged by size:
2130 2109 1697 226 152 20
AseI AT'TA_AT
Cuts at: 160 1032 4888 160
Size: 872 3856 1606
Fragments arranged by size:
3856 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
AvaI C'yCGr_G
Cuts at: 1870 1943 3659 1870
Size: 73 1716 4545
Fragments arranged by size:
4545 1716 73
BamHI G'GATC_C
Cuts at: 2433 3664 4031 2433
Size: 1231 367 4736
Fragments arranged by size:
4736 1231 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3655 5110 618
Size: 325 217 627 1868 1455 1842
Fragments arranged by size:
1868 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2353 3653 1391
Size: 962 1300 4072
Fragments arranged by size:
4072 1300 962
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2253 2633 928
Size: 971 354 380 4629
Fragments arranged by size:
4629 971 380 354
BbvCI CC'TCA_GC
Cuts at: 2127 2127
Size: 6334
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2861 2895 4525 4559 1903
Size: 34 924 34 1630 34 3678
Fragments arranged by size:
3678 1630 924 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2620 4226 5436 2620
Size: 1606 1210 3518
Fragments arranged by size:
3518 1606 1210
BclI T'GATC_A
Cuts at: 1548 2226 1548
Size: 678 5656
BfrBI ATG'CAT
Cuts at: 2210 2210
Size: 6334
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4945 136
Size: 107 122 71 631 605 3273 1525
Fragments arranged by size:
3273 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2368 2495 3205 3269 6329 2368
Size: 127 710 64 3060 2373
Fragments arranged by size:
3060 2373 710 127 64
BlpI GC'TnA_GC
Cuts at: 3751 3751
Size: 6334
Bme1580I G_kGCm'C
Cuts at: 1790 3432 4395 5551 1790
Size: 1642 963 1156 2573
Fragments arranged by size:
2573 1642 1156 963
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6334
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3400 3435 4065
Size: 416 53 83 399 24 1983 35 630
Cuts at: 4065 5023 442
Size: 958 1753
Fragments arranged by size:
1983 1753 958 630 416 399 83 53
35 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4994 1239
Size: 3755 2579
Bpu10I CC'TnA_GC
Cuts at: 2127 2303 2127
Size: 176 6158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4459 5324 5622 4459
Size: 865 298 5171
Fragments arranged by size:
5171 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4997 882
Size: 617 3498 2219
Fragments arranged by size:
3498 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4125 493
Size: 711 2921 2702
Fragments arranged by size:
2921 2702 711
BsaBI GATnn'nnATC
Cuts at: 6328 6328
Size: 6334
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4141 4523 5158 275
Size: 53 83 186 936 2608 382 635 1451
Fragments arranged by size:
2608 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4767 5165 5439 5586 5997 4767
Size: 398 274 147 411 5104
Fragments arranged by size:
5104 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2563 2593 2758 2788 3109
Size: 30 165 30 1160 30 165 30 321
Cuts at: 3109 3139 1178
Size: 30 4373
Fragments arranged by size:
4373 1160 321 165 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2396 2425 2435 1186
Size: 1210 29 10 5085
Fragments arranged by size:
5085 1210 29 10
BseYI C'CCAG_C
Cuts at: 2805 5537 6033 2805
Size: 2732 496 3106
Fragments arranged by size:
3106 2732 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3317 1163
Size: 573 1581 4180
Fragments arranged by size:
4180 1581 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3784 4545 5573 1540
Size: 275 186 1783 761 1028 2301
Fragments arranged by size:
2301 1783 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4395 4480 5551 4395
Size: 85 1071 5178
Fragments arranged by size:
5178 1071 85
BsmI GAATG_Cn'
Cuts at: 2109 2210 3845 3938 2109
Size: 101 1635 93 4505
Fragments arranged by size:
4505 1635 101 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2353 3432 3653 4395 4480 5551 1391
Size: 399 563 1079 221 742 85 1071 2174
Fragments arranged by size:
2174 1079 1071 742 563 399 221 85
BspEI T'CCGG_A
Cuts at: 5997 5997
Size: 6334
BspHI T'CATG_A
Cuts at: 2517 4223 5953 2517
Size: 1706 1730 2898
Fragments arranged by size:
2898 1730 1706
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3689 844
Size: 2845 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4221 1394
Size: 429 2398 3507
Fragments arranged by size:
3507 2398 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2655 2828 4829 5003 65
Size: 1183 1407 173 2001 174 1396
Fragments arranged by size:
2001 1407 1396 1183 174 173
BsrFI r'CCGG_y
Cuts at: 1700 1810 4978 5165 1700
Size: 110 3168 187 2869
Fragments arranged by size:
3168 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6334
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6334
BssSI C'ACGA_G
Cuts at: 4394 5406 4394
Size: 1012 5322
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6334
BstBI TT'CG_AA
Cuts at: 5138 5138
Size: 6334
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6334
BstXI CCAn_nnnn'nTGG
Cuts at: 2596 2596
Size: 6334
BstYI r'GATC_y
Cuts at: 1972 2368 2433 2495 3205 3269 3664 4031 4416
Size: 396 65 62 710 64 395 367 385
Cuts at: 4416 4433 5885 5971 5983 6329 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 710 396 395 385 367 346
86 65 64 62 17 12
Bsu36I CC'TnA_GG
Cuts at: 3187 3187
Size: 6334
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4843
Fragments arranged by size:
4843 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5595
Fragments arranged by size:
5595 683 56
BtsI GCAGTG_nn'
Cuts at: 2752 2816 3037 3217 3850 4644 4664 2752
Size: 64 221 180 633 794 20 4422
Fragments arranged by size:
4422 794 633 221 180 64 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2046 2081 529
Size: 35 1482 35 4782
Fragments arranged by size:
4782 1482 35 35
DraI TTT'AAA
Cuts at: 3638 3638
Size: 6334
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5341 809
Size: 507 4025 1802
Fragments arranged by size:
4025 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3781 4670 6299
Size: 54 1425 50 275 18 1951 889 1629
Cuts at: 6299 8
Size: 43
Fragments arranged by size:
1951 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3781 1537
Size: 275 1969 4090
Fragments arranged by size:
4090 1969 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2096 2423 2830 4264 1353
Size: 743 327 407 1434 3423
Fragments arranged by size:
3423 1434 743 407 327
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4921 5295 5441 387
Size: 1064 138 3332 374 146 1280
Fragments arranged by size:
3332 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3402 4397 4994 5781 6049 1239
Size: 393 1770 995 597 787 268 1524
Fragments arranged by size:
1770 1524 995 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2451 2451
Size: 6334
EcoO109I rG'GnC_Cy
Cuts at: 2307 2771 3175 2307
Size: 464 404 5466
Fragments arranged by size:
5466 464 404
EcoRI G'AATT_C
Cuts at: 3643 4021 3643
Size: 378 5956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3313 3313
Size: 6334
FspI TGC'GCA
Cuts at: 4840 4840
Size: 6334
HaeII r_GCGC'y
Cuts at: 1678 5481 1678
Size: 3803 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 2347 2379 3056
Size: 32 135 32 563 32 180 32 677
Cuts at: 3056 3088 4975 5007 5049 5081 1373
Size: 32 1887 32 42 32 2626
Fragments arranged by size:
2626 1887 677 563 180 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3553 3678 3924 669
Size: 988 281 1615 125 246 3079
Fragments arranged by size:
3079 1615 988 281 246 125
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6334
HpaI GTT'AAC
Cuts at: 3924 3924
Size: 6334
KpnI G_GTAC'C
Cuts at: 3659 3659
Size: 6334
MfeI C'AATT_G
Cuts at: 3933 3933
Size: 6334
MluI A'CGCG_T
Cuts at: 6210 6210
Size: 6334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5448 5632 1305
Size: 4143 184 2007
Fragments arranged by size:
4143 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4512
Fragments arranged by size:
4512 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2255 4292 4651 4810 518
Size: 648 1089 2037 359 159 2042
Fragments arranged by size:
2042 2037 1089 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3750 4427 5575 5820 1606
Size: 2144 677 1148 245 2120
Fragments arranged by size:
2144 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4843
Fragments arranged by size:
4843 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6334
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6334
NotI GC'GGCC_GC
Cuts at: 3781 3781
Size: 6334
NsiI A_TGCA'T
Cuts at: 2212 2212
Size: 6334
NspI r_CATG'y
Cuts at: 1132 1229 3692 5237 1132
Size: 97 2463 1545 2229
Fragments arranged by size:
2463 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3021 1348
Size: 1673 4661
PciI A'CATG_T
Cuts at: 5233 5233
Size: 6334
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6334
PmeI GTTT'AAAC
Cuts at: 3638 3638
Size: 6334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4403 4435 5916 5948 4403
Size: 32 1481 32 4789
Fragments arranged by size:
4789 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3175 3175
Size: 6334
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6334
PsiI TTA'TAA
Cuts at: 2733 3904 2733
Size: 1171 5163
PstI C_TGCA'G
Cuts at: 830 3087 3224 3686 830
Size: 2257 137 462 3478
Fragments arranged by size:
3478 2257 462 137
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6334
SalI G'TCGA_C
Cuts at: 1936 3676 1936
Size: 1740 4594
SapI GCTCTTCn'nnn_
Cuts at: 2830 2830
Size: 6334
SbfI CC_TGCA'GG
Cuts at: 3686 3686
Size: 6334
ScaI AGT'ACT
Cuts at: 4582 6294 4582
Size: 1712 4622
SfcI C'TryA_G
Cuts at: 826 1045 3083 3220 3682 4817 5498 5689 826
Size: 219 2038 137 462 1135 681 191 1471
Fragments arranged by size:
2038 1471 1135 681 462 219 191 137
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6334
SmaI CCC'GGG
Cuts at: 1872 3661 1872
Size: 1789 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4438 5339 5601 820
Size: 197 926 2495 901 262 1553
Fragments arranged by size:
2495 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4125 493
Size: 3632 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6334
SphI G_CATG'C
Cuts at: 1132 3692 1132
Size: 2560 3774
SspI AAT'ATT
Cuts at: 5 52 2694 2795 4258 5
Size: 47 2642 101 1463 2081
Fragments arranged by size:
2642 2081 1463 101 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6334
StyI C'CwwG_G
Cuts at: 513 1065 2004 2927 3773 513
Size: 552 939 923 846 3074
Fragments arranged by size:
3074 939 923 846 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2015 4361 4378 4531 4716 5161 325
Size: 1255 435 2346 17 153 185 445 1498
Fragments arranged by size:
2346 1498 1255 445 435 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4580 6292 96
Size: 274 80 33 51 1443 2603 1712 138
Fragments arranged by size:
2603 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2563 2595 6294 6326 2563
Size: 32 3699 32 2571
Fragments arranged by size:
3699 2571 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6334
XbaI T'CTAG_A
Cuts at: 3670 3670
Size: 6334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2500 3074 2500
Size: 574 5760
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6334
XmaI C'CCGG_G
Cuts at: 1870 3659 1870
Size: 1789 4545
XmnI GAAnn'nnTTC
Cuts at: 2694 4463 2694
Size: 1769 4565
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4142 276
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI