Restriction map for
pFN21AE0351
(Circular) (Six-base) MAPSORT of: pFN21AE0351.seq Check: 3837 from: 1 to: 7159
pFN21AE0351 7159 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2890 4301 2890
Size: 1411 5748
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4969 278
Size: 53 83 186 936 3433 2468
Fragments arranged by size:
3433 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2159 4502 1937
Size: 222 2343 4594
Fragments arranged by size:
4594 2343 222
Acc65I G'GTAC_C
Cuts at: 2718 2962 4480 2718
Size: 244 1518 5397
Fragments arranged by size:
5397 1518 244
AclI AA'CG_TT
Cuts at: 1563 5286 5659 1563
Size: 3723 373 3063
Fragments arranged by size:
3723 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2591 2939 3058 3497 4436 5222 6606 6874
Size: 959 348 119 439 939 786 1384 268
Cuts at: 6874 1632
Size: 1917
Fragments arranged by size:
1917 1384 959 939 786 439 348 268
119
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6962
AflIII A'CryG_T
Cuts at: 1203 6058 7035 1203
Size: 4855 977 1327
Fragments arranged by size:
4855 1327 977
AgeI A'CCGG_T
Cuts at: 5990 5990
Size: 7159
AhdI GACnn_n'nnGTC
Cuts at: 5888 5888
Size: 7159
AleI CACnn'nnGTG
Cuts at: 1166 2528 1166
Size: 1362 5797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2839 1184
Size: 1655 5504
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2399 2431 2399
Size: 32 7127
AlwNI CAG_nnn'CTG
Cuts at: 2618 2929 3046 3337 4402 4902 6474 2618
Size: 311 117 291 1065 500 1572 3303
Fragments arranged by size:
3303 1572 1065 500 311 291 117
ApaI G_GGCC'C
Cuts at: 4081 4081
Size: 7159
ApaLI G'TGCA_C
Cuts at: 2843 5216 6372 2843
Size: 2373 1156 3630
Fragments arranged by size:
3630 2373 1156
ApoI r'AATT_y
Cuts at: 1493 4468 4694 4846 6955 1493
Size: 2975 226 152 2109 1697
Fragments arranged by size:
2975 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5713 160
Size: 872 4681 1606
Fragments arranged by size:
4681 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7159
AvaI C'yCGr_G
Cuts at: 1870 1943 2478 3000 3168 3706 4080 4098 4169
Size: 73 535 522 168 538 374 18 71
Cuts at: 4169 4484 1870
Size: 315 4545
Fragments arranged by size:
4545 538 535 522 374 315 168 73
71 18
BamHI G'GATC_C
Cuts at: 3546 4489 4856 3546
Size: 943 367 5849
Fragments arranged by size:
5849 943 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2667 2718 2962 2996 4385
Size: 325 217 627 880 51 244 34 1389
Cuts at: 4385 4480 5935 618
Size: 95 1455 1842
Fragments arranged by size:
1842 1455 1389 880 627 325 244 217
95 51 34
BanII G_rGCy'C
Cuts at: 1391 2681 3507 3558 3696 3927 4081 4260 4478
Size: 1290 826 51 138 231 154 179 218
Cuts at: 4478 1391
Size: 4072
Fragments arranged by size:
4072 1290 826 231 218 179 154 138
51
BbeI G_GCGC'C
Cuts at: 2671 2671
Size: 7159
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4338 928
Size: 971 2439 3749
Fragments arranged by size:
3749 2439 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4168 4202 5350 5384 1903
Size: 34 2231 34 1148 34 3678
Fragments arranged by size:
3678 2231 1148 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5051 6261 5051
Size: 1210 5949
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7159
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2737 5770 136
Size: 107 122 71 631 605 1065 3033 1525
Fragments arranged by size:
3033 1525 1065 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7154 7154
Size: 7159
BlpI GC'TnA_GC
Cuts at: 2273 2660 3252 4576 2273
Size: 387 592 1324 4856
Fragments arranged by size:
4856 1324 592 387
Bme1580I G_kGCm'C
Cuts at: 1790 2612 2733 2847 3001 4081 4453 5220 6376
Size: 822 121 114 154 1080 372 767 1156
Cuts at: 6376 1790
Size: 2573
Fragments arranged by size:
2573 1156 1080 822 767 372 154 121
114
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7159
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4890 5848 442
Size: 416 53 83 399 24 3473 958 1753
Fragments arranged by size:
3473 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 2288 2573 2579 2665 3167 3215 3260
Size: 872 177 285 6 86 502 48 45
Cuts at: 3260 3320 3440 3604 3746 3806 3815 4244 5819
Size: 60 120 164 142 60 9 429 1575
Cuts at: 5819 1239
Size: 2579
Fragments arranged by size:
2579 1575 872 502 429 285 177 164
142 120 86 60 60 48 45 9
6
Bpu10I CC'TnA_GC
Cuts at: 2885 3501 2885
Size: 616 6543
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2795 3412 5284 6149 6447 2795
Size: 617 1872 865 298 3507
Fragments arranged by size:
3507 1872 865 617 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2937 5822 882
Size: 617 1438 2885 2219
Fragments arranged by size:
2885 2219 1438 617
BsaAI yAC'GTr
Cuts at: 493 1204 2143 3096 4950 493
Size: 711 939 953 1854 2702
Fragments arranged by size:
2702 1854 953 939 711
BsaBI GATnn'nnATC
Cuts at: 2502 7153 2502
Size: 4651 2508
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2668 3945 4966 5348
Size: 53 83 186 936 1135 1277 1021 382
Cuts at: 5348 5983 275
Size: 635 1451
Fragments arranged by size:
1451 1277 1135 1021 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2364 4422 5592 5990 6264 6411 6822 2364
Size: 2058 1170 398 274 147 411 2701
Fragments arranged by size:
2701 2058 1170 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 3581 3611 3719
Size: 30 165 30 597 30 1551 30 108
Cuts at: 3719 3749 3955 3985 1178
Size: 30 206 30 4352
Fragments arranged by size:
4352 1551 597 206 165 108 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2411 2414 2689 2846 2885 3006 3556 3706
Size: 1225 3 275 157 39 121 550 150
Cuts at: 3706 1186
Size: 4639
Fragments arranged by size:
4639 1225 550 275 157 150 121 39
3
BseYI C'CCAG_C
Cuts at: 2109 2714 3046 3902 4109 6362 6858 2109
Size: 605 332 856 207 2253 496 2410
Fragments arranged by size:
2410 2253 856 605 496 332 207
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2886 3296 3821 4172 4391 1163
Size: 573 1150 410 525 351 219 3931
Fragments arranged by size:
3931 1150 573 525 410 351 219
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2163 4609 5370 6398 1540
Size: 275 186 162 2446 761 1028 2301
Fragments arranged by size:
2446 2301 1028 761 275 186 162
BsiHKAI G_wGCw'C
Cuts at: 2525 2681 2847 3177 3507 4260 5220 5305 6376
Size: 156 166 330 330 753 960 85 1071
Cuts at: 6376 2525
Size: 3308
Fragments arranged by size:
3308 1071 960 753 330 330 166 156
85
BsmI GAATG_Cn'
Cuts at: 4145 4670 4763 4145
Size: 525 93 6541
Fragments arranged by size:
6541 525 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2525 2612 2681 2733 2847 3001 3177
Size: 399 735 87 69 52 114 154 176
Cuts at: 3177 3507 3558 3696 3927 4081 4260 4453 4478
Size: 330 51 138 231 154 179 193 25
Cuts at: 4478 5220 5305 6376 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 735 399 330 231 193
179 176 154 154 138 114 87 85
69 52 51 25
BspEI T'CCGG_A
Cuts at: 2364 6822 2364
Size: 4458 2701
BspHI T'CATG_A
Cuts at: 5048 6778 5048
Size: 1730 5429
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2072 2112 2890 2955 3615 4301 4514 844
Size: 1228 40 778 65 660 686 213 3489
Fragments arranged by size:
3489 1228 778 686 660 213 65 40
BsrBI CCG'CTC
Cuts at: 1394 1823 2664 5046 1394
Size: 429 841 2382 3507
Fragments arranged by size:
3507 2382 841 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2695 5654 5828 65
Size: 1183 1447 2959 174 1396
Fragments arranged by size:
2959 1447 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5803 5990 1700
Size: 110 3993 187 2869
Fragments arranged by size:
3993 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7159
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7159
BssSI C'ACGA_G
Cuts at: 3733 5219 6231 3733
Size: 1486 1012 4661
Fragments arranged by size:
4661 1486 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2929 794
Size: 2135 5024
BstBI TT'CG_AA
Cuts at: 5963 5963
Size: 7159
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7159
BstYI r'GATC_y
Cuts at: 1972 3546 3561 4276 4489 4856 5241 5258 6710
Size: 1574 15 715 213 367 385 17 1452
Cuts at: 6710 6796 6808 7154 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1574 1452 715 385 367 346 213
86 17 15 12
Bsu36I CC'TnA_GG
Cuts at: 2672 2735 2829 4116 4403 2672
Size: 63 94 1287 287 5428
Fragments arranged by size:
5428 1287 287 94 63
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5668
Fragments arranged by size:
5668 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6420
Fragments arranged by size:
6420 683 56
BtsI GCAGTG_nn'
Cuts at: 4049 4675 5469 5489 4049
Size: 626 794 20 5719
Fragments arranged by size:
5719 794 626 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7159
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3256 3291 529
Size: 35 2692 35 4397
Fragments arranged by size:
4397 2692 35 35
DraI TTT'AAA
Cuts at: 3772 4463 3772
Size: 691 6468
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6166 809
Size: 507 4850 1802
Fragments arranged by size:
4850 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2035 2838 2907
Size: 54 1425 50 275 18 205 803 69
Cuts at: 2907 2982 3022 3180 3316 3986 4267 4606 5495
Size: 75 40 158 136 670 281 339 889
Cuts at: 5495 7124 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 803 670 339 281 275
205 158 136 75 69 54 50 43
40 18
EagI C'GGCC_G
Cuts at: 1537 1812 4606 1537
Size: 275 2794 4090
Fragments arranged by size:
4090 2794 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3512 4154 5089 1353
Size: 2159 642 935 3423
Fragments arranged by size:
3423 2159 935 642
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5746 6120 6266 387
Size: 1064 138 4157 374 146 1280
Fragments arranged by size:
4157 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2679 3505 4258 2679
Size: 826 753 5580
Fragments arranged by size:
5580 826 753
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2288 2573 2579 2591 2665 2939
Size: 393 479 177 285 6 12 74 274
Cuts at: 2939 3058 3167 3215 3260 3320 3440 3497 3604
Size: 119 109 48 45 60 120 57 107
Cuts at: 3604 3746 3806 3815 4244 4436 5222 5819 6606
Size: 142 60 9 429 192 786 597 787
Cuts at: 6606 6874 1239
Size: 268 1524
Fragments arranged by size:
1524 787 786 597 479 429 393 285
274 268 192 177 142 120 119 109
107 74 60 60 57 48 45 12
9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2921 2951 2921
Size: 30 7129
EcoO109I rG'GnC_Cy
Cuts at: 2086 2577 2739 2914 2926 3121 3142 3152 3486
Size: 491 162 175 12 195 21 10 334
Cuts at: 3486 3711 3792 3826 4174 4399 2086
Size: 225 81 34 348 225 4846
Fragments arranged by size:
4846 491 348 334 225 225 195 175
162 81 34 21 12 10
EcoRI G'AATT_C
Cuts at: 4468 4846 4468
Size: 378 6781
FspI TGC'GCA
Cuts at: 5665 5665
Size: 7159
HaeII r_GCGC'y
Cuts at: 1678 2671 3217 3293 3758 4345 6306 1678
Size: 993 546 76 465 587 1961 2531
Fragments arranged by size:
2531 1961 993 587 546 465 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2276 2308 5800
Size: 32 135 32 428 32 244 32 3492
Cuts at: 5800 5832 5874 5906 1373
Size: 32 42 32 2626
Fragments arranged by size:
3492 2626 428 244 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2160 4503 4749 669
Size: 988 281 222 2343 246 3079
Fragments arranged by size:
3079 2343 988 281 246 222
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7159
HpaI GTT'AAC
Cuts at: 4749 4749
Size: 7159
KasI G'GCGC_C
Cuts at: 2667 2667
Size: 7159
KpnI G_GTAC'C
Cuts at: 2722 2966 4484 2722
Size: 244 1518 5397
Fragments arranged by size:
5397 1518 244
MfeI C'AATT_G
Cuts at: 4758 4758
Size: 7159
MluI A'CGCG_T
Cuts at: 7035 7035
Size: 7159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4335 6273 6457 1305
Size: 3030 1938 184 2007
Fragments arranged by size:
3030 2007 1938 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2037 2909 2984 3182 4269
Size: 54 1425 343 205 872 75 198 1087
Cuts at: 4269 10
Size: 2900
Fragments arranged by size:
2900 1425 1087 872 343 205 198 75
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 5117 5476 5635 518
Size: 648 1362 2589 359 159 2042
Fragments arranged by size:
2589 2042 1362 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2155 2528 2714 2935 3835 3910 4198 4575
Size: 549 373 186 221 900 75 288 377
Cuts at: 4575 5252 6400 6645 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 900 677 549 377 373 288
245 221 186 75
NarI GG'CG_CC
Cuts at: 2668 2668
Size: 7159
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5668
Fragments arranged by size:
5668 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7159
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7159
NotI GC'GGCC_GC
Cuts at: 4606 4606
Size: 7159
NspI r_CATG'y
Cuts at: 1132 1229 4517 6062 1132
Size: 97 3288 1545 2229
Fragments arranged by size:
3288 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2389 1348
Size: 1041 6118
PciI A'CATG_T
Cuts at: 6058 6058
Size: 7159
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2638 3031 3325 1108
Size: 1530 393 294 4942
Fragments arranged by size:
4942 1530 393 294
PfoI T'CCnGG_A
Cuts at: 2088 3549 3647 2088
Size: 1461 98 5600
Fragments arranged by size:
5600 1461 98
PmeI GTTT'AAAC
Cuts at: 4463 4463
Size: 7159
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5228 5260 6741 6773 5228
Size: 32 1481 32 5614
Fragments arranged by size:
5614 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2086 2577 3121 3142 3152 3711 2086
Size: 491 544 21 10 559 5534
Fragments arranged by size:
5534 559 544 491 21 10
PshAI GACnn'nnGTC
Cuts at: 1613 2205 1613
Size: 592 6567
PsiI TTA'TAA
Cuts at: 4729 4729
Size: 7159
PspOMI G'GGCC_C
Cuts at: 4077 4077
Size: 7159
PstI C_TGCA'G
Cuts at: 830 2808 2934 2952 3744 4056 4398 4511 830
Size: 1978 126 18 792 312 342 113 3478
Fragments arranged by size:
3478 1978 792 342 312 126 113 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7159
PvuII CAG'CTG
Cuts at: 2155 2714 2935 3835 3910 2155
Size: 559 221 900 75 5404
Fragments arranged by size:
5404 900 559 221 75
SacI G_AGCT'C
Cuts at: 2681 3507 4260 2681
Size: 826 753 5580
Fragments arranged by size:
5580 826 753
SalI G'TCGA_C
Cuts at: 1936 2158 4501 1936
Size: 222 2343 4594
Fragments arranged by size:
4594 2343 222
SanDI GG'GwC_CC
Cuts at: 3121 3121
Size: 7159
SapI GCTCTTCn'nnn_
Cuts at: 3512 3512
Size: 7159
SbfI CC_TGCA'GG
Cuts at: 2952 4398 4511 2952
Size: 1446 113 5600
Fragments arranged by size:
5600 1446 113
ScaI AGT'ACT
Cuts at: 5407 7119 5407
Size: 1712 5447
SfcI C'TryA_G
Cuts at: 826 1045 2150 2804 2930 2948 3740 3850 4052
Size: 219 1105 654 126 18 792 110 202
Cuts at: 4052 4394 4507 5642 6323 6514 826
Size: 342 113 1135 681 191 1471
Fragments arranged by size:
1471 1135 1105 792 681 654 342 219
202 191 126 113 110 18
SfoI GGC'GCC
Cuts at: 2669 2669
Size: 7159
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7159
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7159
SmaI CCC'GGG
Cuts at: 1872 3002 3170 4082 4100 4486 1872
Size: 1130 168 912 18 386 4545
Fragments arranged by size:
4545 1130 912 386 168 18
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2810 3391 5263 6164 6426 820
Size: 197 926 867 581 1872 901 262 1553
Fragments arranged by size:
1872 1553 926 901 867 581 262 197
SnaBI TAC'GTA
Cuts at: 493 4950 493
Size: 4457 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7159
SphI G_CATG'C
Cuts at: 1132 4517 1132
Size: 3385 3774
SrfI GCCC'GGGC
Cuts at: 3002 3002
Size: 7159
SspI AAT'ATT
Cuts at: 5 52 5083 5
Size: 47 5031 2081
Fragments arranged by size:
5031 2081 47
StuI AGG'CCT
Cuts at: 1517 2057 2228 2879 3731 4441 1517
Size: 540 171 651 852 710 4235
Fragments arranged by size:
4235 852 710 651 540 171
StyI C'CwwG_G
Cuts at: 513 1065 2004 2910 3918 4133 4445 4598 513
Size: 552 939 906 1008 215 312 153 3074
Fragments arranged by size:
3074 1008 939 906 552 312 215 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5186 5203 5356 5541 5986 325
Size: 1255 3606 17 153 185 445 1498
Fragments arranged by size:
3606 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4068 5405 7117
Size: 274 80 33 51 1443 2091 1337 1712
Cuts at: 7117 96
Size: 138
Fragments arranged by size:
2091 1712 1443 1337 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2194 2226 3113 3145 3719 3751 3955 3987 7119
Size: 32 887 32 574 32 204 32 3132
Cuts at: 7119 7151 2194
Size: 32 2202
Fragments arranged by size:
3132 2202 887 574 204 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2294 1316
Size: 978 6181
XbaI T'CTAG_A
Cuts at: 4429 4495 4429
Size: 66 7093
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3190 3274 3190
Size: 84 7075
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7159
XmaI C'CCGG_G
Cuts at: 1870 3000 3168 4080 4098 4484 1870
Size: 1130 168 912 18 386 4545
Fragments arranged by size:
4545 1130 912 386 168 18
XmnI GAAnn'nnTTC
Cuts at: 2372 5288 2372
Size: 2916 4243
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4967 276
Size: 53 83 186 936 3433 2468
Fragments arranged by size:
3433 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SexAI SfiI SwaI