Restriction map for
pFN21AE0348
(Circular) (Six-base) MAPSORT of: pFN21AE0348.seq Check: 9246 from: 1 to: 6214
pFN21AE0348 6214 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2251 2843 2851 2976 2251
Size: 592 8 125 5489
Fragments arranged by size:
5489 592 125 8
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4024 278
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2916 3399 3557 1937
Size: 979 483 158 4594
Fragments arranged by size:
4594 979 483 158
Acc65I G'GTAC_C
Cuts at: 3535 3535
Size: 6214
AclI AA'CG_TT
Cuts at: 1563 4341 4714 1563
Size: 2778 373 3063
Fragments arranged by size:
3063 2778 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 2561 4277 5661 5929 1632
Size: 695 234 1716 1384 268 1917
Fragments arranged by size:
1917 1716 1384 695 268 234
AfeI AGC'GCT
Cuts at: 2891 2891
Size: 6214
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6017
AflIII A'CryG_T
Cuts at: 1203 5113 6090 1203
Size: 3910 977 1327
Fragments arranged by size:
3910 1327 977
AgeI A'CCGG_T
Cuts at: 5045 5045
Size: 6214
AhdI GACnn_n'nnGTC
Cuts at: 4943 4943
Size: 6214
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6214
AlwNI CAG_nnn'CTG
Cuts at: 2284 2305 2473 2548 2617 2788 3957 5529 2284
Size: 21 168 75 69 171 1169 1572 2969
Fragments arranged by size:
2969 1572 1169 171 168 75 69 21
ApaI G_GGCC'C
Cuts at: 3464 3464
Size: 6214
ApaLI G'TGCA_C
Cuts at: 3104 4271 5427 3104
Size: 1167 1156 3891
Fragments arranged by size:
3891 1167 1156
ApoI r'AATT_y
Cuts at: 1493 3025 3523 3749 3901 6010 1493
Size: 1532 498 226 152 2109 1697
Fragments arranged by size:
2109 1697 1532 498 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4768 160
Size: 872 3736 1606
Fragments arranged by size:
3736 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
AvaI C'yCGr_G
Cuts at: 1870 1943 2597 3539 1870
Size: 73 654 942 4545
Fragments arranged by size:
4545 942 654 73
BamHI G'GATC_C
Cuts at: 3544 3911 3544
Size: 367 5847
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2408 2802 3017 3258 3535
Size: 325 217 627 621 394 215 241 277
Cuts at: 3535 4990 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 621 394 325 277 241
217 215
BanII G_rGCy'C
Cuts at: 1391 2166 3464 3533 1391
Size: 775 1298 69 4072
Fragments arranged by size:
4072 1298 775 69
BbeI G_GCGC'C
Cuts at: 3262 3262
Size: 6214
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5243
BbvCI CC'TCA_GC
Cuts at: 2654 2654
Size: 6214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3158 3192 4405 4439 1903
Size: 34 50 34 1137 34 1213 34 3678
Fragments arranged by size:
3678 1213 1137 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4106 5316 4106
Size: 1210 5004
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6214
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4825 136
Size: 107 122 71 631 605 3153 1525
Fragments arranged by size:
3153 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6209 6209
Size: 6214
BlpI GC'TnA_GC
Cuts at: 2466 2684 3250 3631 2466
Size: 218 566 381 5049
Fragments arranged by size:
5049 566 381 218
Bme1580I G_kGCm'C
Cuts at: 1790 2411 3108 3340 3464 4275 5431 1790
Size: 621 697 232 124 811 1156 2573
Fragments arranged by size:
2573 1156 811 697 621 232 124
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6214
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3166 3175 3945
Size: 416 53 83 399 24 1749 9 770
Cuts at: 3945 4903 442
Size: 958 1753
Fragments arranged by size:
1753 1749 958 770 416 399 83 53
24 9
BmtI G_CTAG'C
Cuts at: 1056 2021 1056
Size: 965 5249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2173 2192 2294 2372 2552 2555 2639 2731
Size: 934 19 102 78 180 3 84 92
Cuts at: 2731 3372 4874 1239
Size: 641 1502 2579
Fragments arranged by size:
2579 1502 934 641 180 102 92 84
78 19 3
Bpu10I CC'TnA_GC
Cuts at: 2654 2654
Size: 6214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2359 4339 5204 5502 2359
Size: 1980 865 298 3071
Fragments arranged by size:
3071 1980 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2191 2504 2615 3060 4877 882
Size: 617 692 313 111 445 1817 2219
Fragments arranged by size:
2219 1817 692 617 445 313 111
BsaAI yAC'GTr
Cuts at: 493 1204 4005 493
Size: 711 2801 2702
Fragments arranged by size:
2801 2702 711
BsaBI GATnn'nnATC
Cuts at: 6208 6208
Size: 6214
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3259 3369 3396 4021
Size: 53 83 186 936 1726 110 27 625
Cuts at: 4021 4403 5038 275
Size: 382 635 1451
Fragments arranged by size:
1726 1451 936 635 625 382 186 110
83 53 27
BsaWI w'CCGG_w
Cuts at: 4647 5045 5319 5466 5877 4647
Size: 398 274 147 411 4984
Fragments arranged by size:
4984 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5989
Fragments arranged by size:
5989 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2651 1186
Size: 844 621 4749
Fragments arranged by size:
4749 844 621
BseYI C'CCAG_C
Cuts at: 2013 2256 2404 3070 3074 3088 3464 5417 5913
Size: 243 148 666 4 14 376 1953 496
Cuts at: 5913 2013
Size: 2314
Fragments arranged by size:
2314 1953 666 496 376 243 148 14
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 2898 1163
Size: 573 483 679 4479
Fragments arranged by size:
4479 679 573 483
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3664 4425 5453 1540
Size: 275 186 1663 761 1028 2301
Fragments arranged by size:
2301 1663 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3084 3108 4275 4360 5431 3084
Size: 24 1167 85 1071 3867
Fragments arranged by size:
3867 1167 1071 85 24
BsmI GAATG_Cn'
Cuts at: 3725 3818 3725
Size: 93 6121
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2166 2411 3084 3108 3340 3464 3533
Size: 399 376 245 673 24 232 124 69
Cuts at: 3533 4275 4360 5431 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 673 399 376 245 232
124 85 69 24
BspEI T'CCGG_A
Cuts at: 5877 5877
Size: 6214
BspHI T'CATG_A
Cuts at: 4103 5833 4103
Size: 1730 4484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2251 2347 2843 2851 2976 3569 844
Size: 1407 96 496 8 125 593 3489
Fragments arranged by size:
3489 1407 593 496 125 96 8
BsrBI CCG'CTC
Cuts at: 1394 1823 4101 1394
Size: 429 2278 3507
Fragments arranged by size:
3507 2278 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3100 4709 4883 65
Size: 1183 1852 1609 174 1396
Fragments arranged by size:
1852 1609 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4858 5045 1700
Size: 110 3048 187 2869
Fragments arranged by size:
3048 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6214
BssHII G'CGCG_C
Cuts at: 1925 2592 1925
Size: 667 5547
BssSI C'ACGA_G
Cuts at: 3167 4274 5286 3167
Size: 1107 1012 4095
Fragments arranged by size:
4095 1107 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2284 2776 2809 794
Size: 1490 492 33 4199
Fragments arranged by size:
4199 1490 492 33
BstBI TT'CG_AA
Cuts at: 5018 5018
Size: 6214
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6214
BstXI CCAn_nnnn'nTGG
Cuts at: 2065 2533 2894 2065
Size: 468 361 5385
Fragments arranged by size:
5385 468 361
BstYI r'GATC_y
Cuts at: 1972 2439 2883 3544 3911 4296 4313 5765 5851
Size: 467 444 661 367 385 17 1452 86
Cuts at: 5851 5863 6209 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 661 467 444 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2181 2181
Size: 6214
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5662
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5475
Fragments arranged by size:
5475 683 56
BtsI GCAGTG_nn'
Cuts at: 2231 2282 2306 2430 2983 3211 3473 3730 4524
Size: 51 24 124 553 228 262 257 794
Cuts at: 4524 4544 2231
Size: 20 3901
Fragments arranged by size:
3901 794 553 262 257 228 124 51
24 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6214
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6179
DraI TTT'AAA
Cuts at: 3518 3518
Size: 6214
DraIII CAC_nnn'GTG
Cuts at: 2301 2809 3479 2301
Size: 508 670 5036
Fragments arranged by size:
5036 670 508
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2518 5221 809
Size: 507 1202 2703 1802
Fragments arranged by size:
2703 1802 1202 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2125 2223 2643
Size: 54 1425 50 275 18 295 98 420
Cuts at: 2643 3661 4550 6179 8
Size: 1018 889 1629 43
Fragments arranged by size:
1629 1425 1018 889 420 295 275 98
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3661 1537
Size: 275 1849 4090
Fragments arranged by size:
4090 1849 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4144 1353
Size: 2791 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2105 4801 5175 5321 387
Size: 1064 138 516 2696 374 146 1280
Fragments arranged by size:
2696 1280 1064 516 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2173 2192 2294 2327 2372 2552 2555
Size: 393 541 19 102 33 45 180 3
Cuts at: 2555 2561 2639 2731 3372 4277 4874 5661 5929
Size: 6 78 92 641 905 597 787 268
Cuts at: 5929 1239
Size: 1524
Fragments arranged by size:
1524 905 787 641 597 541 393 268
180 102 92 78 45 33 19 6
3
EcoNI CCTnn'n_nnAGG
Cuts at: 2756 2756
Size: 6214
EcoO109I rG'GnC_Cy
Cuts at: 2177 2614 3460 3461 2177
Size: 437 846 1 4930
Fragments arranged by size:
4930 846 437 1
EcoRI G'AATT_C
Cuts at: 3523 3901 3523
Size: 378 5836
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 6214
FspI TGC'GCA
Cuts at: 4720 4720
Size: 6214
HaeII r_GCGC'y
Cuts at: 1678 2700 2893 3007 3262 5361 1678
Size: 1022 193 114 255 2099 2531
Fragments arranged by size:
2531 2099 1022 255 193 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4855 4887 4929 4961 1373
Size: 32 135 32 3283 32 42 32 2626
Fragments arranged by size:
3283 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3558 3804 669
Size: 988 281 1620 246 3079
Fragments arranged by size:
3079 1620 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6214
HpaI GTT'AAC
Cuts at: 3804 3804
Size: 6214
KasI G'GCGC_C
Cuts at: 3258 3258
Size: 6214
KpnI G_GTAC'C
Cuts at: 3539 3539
Size: 6214
MfeI C'AATT_G
Cuts at: 3813 3813
Size: 6214
MluI A'CGCG_T
Cuts at: 6090 6090
Size: 6214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2223 5328 5512 1305
Size: 918 3105 184 2007
Fragments arranged by size:
3105 2007 918 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2225 2645 10
Size: 54 1425 343 393 420 3579
Fragments arranged by size:
3579 1425 420 393 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2063 2927 4172 4531 4690 518
Size: 648 897 864 1245 359 159 2042
Fragments arranged by size:
2042 1245 897 864 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2272 2545 2785 3034 3074 3246 3444 3630
Size: 666 273 240 249 40 172 198 186
Cuts at: 3630 4307 5455 5700 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 666 273 249 245 240
198 186 172 40
NarI GG'CG_CC
Cuts at: 3259 3259
Size: 6214
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5662
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6214
NheI G'CTAG_C
Cuts at: 1052 2017 1052
Size: 965 5249
NotI GC'GGCC_GC
Cuts at: 3661 3661
Size: 6214
NspI r_CATG'y
Cuts at: 1132 1229 3572 5117 1132
Size: 97 2343 1545 2229
Fragments arranged by size:
2343 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6214
PciI A'CATG_T
Cuts at: 5113 5113
Size: 6214
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2788 1108
Size: 1680 4534
PfoI T'CCnGG_A
Cuts at: 2868 2868
Size: 6214
PmeI GTTT'AAAC
Cuts at: 3518 3518
Size: 6214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2849 2881 4283 4315 5796 5828 2849
Size: 32 1402 32 1481 32 3235
Fragments arranged by size:
3235 1481 1402 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3321 1613
Size: 1708 4506
PsiI TTA'TAA
Cuts at: 3784 3784
Size: 6214
PspOMI G'GGCC_C
Cuts at: 3460 3460
Size: 6214
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3203 3235 3203
Size: 32 6182
PstI C_TGCA'G
Cuts at: 830 2031 2289 2760 2973 3282 3566 830
Size: 1201 258 471 213 309 284 3478
Fragments arranged by size:
3478 1201 471 309 284 258 213
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6214
PvuII CAG'CTG
Cuts at: 2272 2545 2785 3074 2272
Size: 273 240 289 5412
Fragments arranged by size:
5412 289 273 240
SalI G'TCGA_C
Cuts at: 1936 3556 1936
Size: 1620 4594
SbfI CC_TGCA'GG
Cuts at: 2760 3566 2760
Size: 806 5408
ScaI AGT'ACT
Cuts at: 4462 6174 4462
Size: 1712 4502
SexAI A'CCwGG_T
Cuts at: 2805 2805
Size: 6214
SfcI C'TryA_G
Cuts at: 826 1045 2027 2131 2285 2552 2756 2969 3278
Size: 219 982 104 154 267 204 213 309
Cuts at: 3278 3562 4697 5378 5569 826
Size: 284 1135 681 191 1471
Fragments arranged by size:
1471 1135 982 681 309 284 267 219
213 204 191 154 104
SfoI GGC'GCC
Cuts at: 3260 3260
Size: 6214
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6214
SmaI CCC'GGG
Cuts at: 1872 3541 1872
Size: 1669 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2338 2597 4318 5219 5481 820
Size: 197 926 395 259 1721 901 262 1553
Fragments arranged by size:
1721 1553 926 901 395 262 259 197
SnaBI TAC'GTA
Cuts at: 493 4005 493
Size: 3512 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6214
SphI G_CATG'C
Cuts at: 1132 3572 1132
Size: 2440 3774
SspI AAT'ATT
Cuts at: 5 52 4138 5
Size: 47 4086 2081
Fragments arranged by size:
4086 2081 47
StuI AGG'CCT
Cuts at: 1517 3173 1517
Size: 1656 4558
StyI C'CwwG_G
Cuts at: 513 1065 3653 513
Size: 552 2588 3074
Fragments arranged by size:
3074 2588 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2270 2468 4241 4258 4411 4596 5041
Size: 1255 690 198 1773 17 153 185 445
Cuts at: 5041 325
Size: 1498
Fragments arranged by size:
1773 1498 1255 690 445 198 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3423 4460 6172
Size: 274 80 33 51 1443 1446 1037 1712
Cuts at: 6172 96
Size: 138
Fragments arranged by size:
1712 1446 1443 1037 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2100 2132 6174 6206 2100
Size: 32 4042 32 2108
Fragments arranged by size:
4042 2108 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6214
XbaI T'CTAG_A
Cuts at: 3550 3550
Size: 6214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2062 2401 2530 3347 2062
Size: 339 129 817 4929
Fragments arranged by size:
4929 817 339 129
XhoI C'TCGA_G
Cuts at: 1943 2597 1943
Size: 654 5560
XmaI C'CCGG_G
Cuts at: 1870 3539 1870
Size: 1669 4545
XmnI GAAnn'nnTTC
Cuts at: 2957 4343 2957
Size: 1386 4828
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4022 276
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI RsrII SacI SacII
SanDI SapI SfiI SrfI SwaI