Restriction map for
pFN21AE0347
(Circular) (Six-base) MAPSORT of: pFN21AE0347.seq Check: 9887 from: 1 to: 7264
pFN21AE0347 7264 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2605 4044 2605
Size: 1439 5825
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5074 278
Size: 53 83 186 936 3538 2468
Fragments arranged by size:
3538 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4607 1937
Size: 2670 4594
Acc65I G'GTAC_C
Cuts at: 4585 4585
Size: 7264
AclI AA'CG_TT
Cuts at: 1563 5391 5764 1563
Size: 3828 373 3063
Fragments arranged by size:
3828 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3011 3252 3542 3837 4562 5327 6711 6979
Size: 1379 241 290 295 725 765 1384 268
Cuts at: 6979 1632
Size: 1917
Fragments arranged by size:
1917 1384 1379 765 725 295 290 268
241
AfeI AGC'GCT
Cuts at: 3596 3596
Size: 7264
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7067
AflIII A'CryG_T
Cuts at: 1203 3405 3579 4455 6163 7140 1203
Size: 2202 174 876 1708 977 1327
Fragments arranged by size:
2202 1708 1327 977 876 174
AgeI A'CCGG_T
Cuts at: 6095 6095
Size: 7264
AhdI GACnn_n'nnGTC
Cuts at: 5993 5993
Size: 7264
AleI CACnn'nnGTG
Cuts at: 1166 4454 1166
Size: 3288 3976
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7264
AlwNI CAG_nnn'CTG
Cuts at: 4489 5007 6579 4489
Size: 518 1572 5174
Fragments arranged by size:
5174 1572 518
ApaI G_GGCC'C
Cuts at: 3013 3926 4201 3013
Size: 913 275 6076
Fragments arranged by size:
6076 913 275
ApaLI G'TGCA_C
Cuts at: 4408 4442 5321 6477 4408
Size: 34 879 1156 5195
Fragments arranged by size:
5195 1156 879 34
ApoI r'AATT_y
Cuts at: 1493 4025 4573 4799 4951 7060 1493
Size: 2532 548 226 152 2109 1697
Fragments arranged by size:
2532 2109 1697 548 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5818 160
Size: 872 4786 1606
Fragments arranged by size:
4786 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7264
AvaI C'yCGr_G
Cuts at: 1870 1943 2376 4223 4265 4589 1870
Size: 73 433 1847 42 324 4545
Fragments arranged by size:
4545 1847 433 324 73 42
BamHI G'GATC_C
Cuts at: 4594 4961 4594
Size: 367 6897
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3615 4475 4585 6040 618
Size: 325 217 627 1828 860 110 1455 1842
Fragments arranged by size:
1842 1828 1455 860 627 325 217 110
BanII G_rGCy'C
Cuts at: 1391 3013 3366 3567 3926 4201 4419 4583 1391
Size: 1622 353 201 359 275 218 164 4072
Fragments arranged by size:
4072 1622 359 353 275 218 201 164
BbeI G_GCGC'C
Cuts at: 3619 3619
Size: 7264
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2064 3151 928
Size: 971 165 1087 5041
Fragments arranged by size:
5041 1087 971 165
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2442 2476 2804 2838 4399 4433 5455
Size: 34 505 34 328 34 1561 34 1022
Cuts at: 5455 5489 1903
Size: 34 3678
Fragments arranged by size:
3678 1561 1022 505 328 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5156 6366 5156
Size: 1210 6054
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7264
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2388 5875 136
Size: 107 122 71 631 605 716 3487 1525
Fragments arranged by size:
3487 1525 716 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7259 7259
Size: 7264
BlpI GC'TnA_GC
Cuts at: 2195 4681 2195
Size: 2486 4778
Bme1580I G_kGCm'C
Cuts at: 1790 3013 3533 3926 4201 4412 4446 4480 5325
Size: 1223 520 393 275 211 34 34 845
Cuts at: 5325 6481 1790
Size: 1156 2573
Fragments arranged by size:
2573 1223 1156 845 520 393 275 211
34 34
BmgBI CAC'GTC
Cuts at: 1318 3886 4456 1318
Size: 2568 570 4126
Fragments arranged by size:
4126 2568 570
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2252 2852 3042
Size: 416 53 83 399 24 835 600 190
Cuts at: 3042 4995 5953 442
Size: 1953 958 1753
Fragments arranged by size:
1953 1753 958 835 600 416 399 190
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2323 2942 2988 3536 3589 4166 4525
Size: 899 185 619 46 548 53 577 359
Cuts at: 4525 5924 1239
Size: 1399 2579
Fragments arranged by size:
2579 1399 899 619 577 548 359 185
53 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3185 3413 5389 6254 6552 3185
Size: 228 1976 865 298 3897
Fragments arranged by size:
3897 1976 865 298 228
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2824 5927 882
Size: 617 1325 3103 2219
Fragments arranged by size:
3103 2219 1325 617
BsaAI yAC'GTr
Cuts at: 493 1204 3877 5055 493
Size: 711 2673 1178 2702
Fragments arranged by size:
2702 2673 1178 711
BsaBI GATnn'nnATC
Cuts at: 3243 3423 7258 3243
Size: 180 3835 3249
Fragments arranged by size:
3835 3249 180
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2043 2543 3606 3616
Size: 53 83 186 936 510 500 1063 10
Cuts at: 3616 5071 5453 6088 275
Size: 1455 382 635 1451
Fragments arranged by size:
1455 1451 1063 936 635 510 500 382
186 83 53 10
BsaWI w'CCGG_w
Cuts at: 5697 6095 6369 6516 6927 5697
Size: 398 274 147 411 6034
Fragments arranged by size:
6034 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2081 2111 2321 2351 1178
Size: 30 165 30 678 30 210 30 6091
Fragments arranged by size:
6091 678 210 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 3842 1186
Size: 865 1791 4608
Fragments arranged by size:
4608 1791 865
BseYI C'CCAG_C
Cuts at: 2305 2489 2957 4492 6467 6963 2305
Size: 184 468 1535 1975 496 2606
Fragments arranged by size:
2606 1975 1535 496 468 184
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3117 3421 1163
Size: 573 1381 304 5006
Fragments arranged by size:
5006 1381 573 304
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3546 4076 4714 5475 6503 1540
Size: 275 186 1545 530 638 761 1028 2301
Fragments arranged by size:
2301 1545 1028 761 638 530 275 186
BsiHKAI G_wGCw'C
Cuts at: 4412 4419 4446 5325 5410 6481 4412
Size: 7 27 879 85 1071 5195
Fragments arranged by size:
5195 1071 879 85 27 7
BsmI GAATG_Cn'
Cuts at: 4775 4868 4775
Size: 93 7171
BsmBI CGTCTCn'nnnn_
Cuts at: 2437 2896 2437
Size: 459 6805
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3013 3366 3533 3567 3926 4201 4412
Size: 399 1223 353 167 34 359 275 211
Cuts at: 4412 4419 4446 4480 4583 5325 5410 6481 1391
Size: 7 27 34 103 742 85 1071 2174
Fragments arranged by size:
2174 1223 1071 742 399 359 353 275
211 167 103 85 34 34 27 7
BspEI T'CCGG_A
Cuts at: 6927 6927
Size: 7264
BspHI T'CATG_A
Cuts at: 4380 5153 6883 4380
Size: 773 1730 4761
Fragments arranged by size:
4761 1730 773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2605 4044 4619 844
Size: 1761 1439 575 3489
Fragments arranged by size:
3489 1761 1439 575
BsrBI CCG'CTC
Cuts at: 1394 1823 5151 1394
Size: 429 3328 3507
Fragments arranged by size:
3507 3328 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3834 5759 5933 65
Size: 1183 2586 1925 174 1396
Fragments arranged by size:
2586 1925 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2045 2382 3492 4011 4071 5908 6095
Size: 110 235 337 1110 519 60 1837 187
Cuts at: 6095 1700
Size: 2869
Fragments arranged by size:
2869 1837 1110 519 337 235 187 110
60
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7264
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7264
BssSI C'ACGA_G
Cuts at: 2687 3481 3857 5324 6336 2687
Size: 794 376 1467 1012 3615
Fragments arranged by size:
3615 1467 1012 794 376
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3444 794
Size: 2650 4614
BstBI TT'CG_AA
Cuts at: 4023 6068 4023
Size: 2045 5219
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7264
BstXI CCAn_nnnn'nTGG
Cuts at: 2795 2795
Size: 7264
BstYI r'GATC_y
Cuts at: 1972 4425 4594 4961 5346 5363 6815 6901 6913
Size: 2453 169 367 385 17 1452 86 12
Cuts at: 6913 7259 1972
Size: 346 1977
Fragments arranged by size:
2453 1977 1452 385 367 346 169 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2415 2474 2727 3013 4471 513
Size: 552 1350 59 253 286 1458 3306
Fragments arranged by size:
3306 1458 1350 552 286 253 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2235 2814 3739 487
Size: 683 56 1009 579 925 4012
Fragments arranged by size:
4012 1009 925 683 579 56
BtsI GCAGTG_nn'
Cuts at: 4780 5574 5594 4780
Size: 794 20 6450
Fragments arranged by size:
6450 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7264
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7229
DraI TTT'AAA
Cuts at: 4568 4568
Size: 7264
DraIII CAC_nnn'GTG
Cuts at: 3444 3444
Size: 7264
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2258 6271 809
Size: 507 942 4013 1802
Fragments arranged by size:
4013 1802 942 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2477 2871 3543
Size: 54 1425 50 275 18 647 394 672
Cuts at: 3543 4073 4711 5600 7229 8
Size: 530 638 889 1629 43
Fragments arranged by size:
1629 1425 889 672 647 638 530 394
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3543 4073 4711 1537
Size: 275 1731 530 638 4090
Fragments arranged by size:
4090 1731 638 530 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2279 4022 4535 5194 1353
Size: 926 1743 513 659 3423
Fragments arranged by size:
3423 1743 926 659 513
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3960 5851 6225 6371 387
Size: 1064 138 2371 1891 374 146 1280
Fragments arranged by size:
2371 1891 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4417 4417
Size: 7264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2323 2942 2988 3011 3252 3536
Size: 393 506 185 619 46 23 241 284
Cuts at: 3536 3542 3589 3837 4166 4525 4562 5327 5924
Size: 6 47 248 329 359 37 765 597
Cuts at: 5924 6711 6979 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 765 619 597 506 393 359
329 284 268 248 241 185 47 46
37 23 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2579 3998 2579
Size: 1419 5845
EcoO109I rG'GnC_Cy
Cuts at: 2079 2436 2683 3043 3922 4197 4500 2079
Size: 357 247 360 879 275 303 4843
Fragments arranged by size:
4843 879 360 357 303 275 247
EcoRI G'AATT_C
Cuts at: 4573 4951 4573
Size: 378 6886
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4567 4567
Size: 7264
FspI TGC'GCA
Cuts at: 5770 5770
Size: 7264
HaeII r_GCGC'y
Cuts at: 1678 3199 3598 3619 6411 1678
Size: 1521 399 21 2792 2531
Fragments arranged by size:
2792 2531 1521 399 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 5905 5937 5979
Size: 32 135 32 509 32 3792 32 42
Cuts at: 5979 6011 1373
Size: 32 2626
Fragments arranged by size:
3792 2626 509 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2074 4608 4854 669
Size: 988 281 136 2534 246 3079
Fragments arranged by size:
3079 2534 988 281 246 136
HindIII A'AGCT_T
Cuts at: 748 3554 748
Size: 2806 4458
HpaI GTT'AAC
Cuts at: 4854 4854
Size: 7264
KasI G'GCGC_C
Cuts at: 3615 3615
Size: 7264
KpnI G_GTAC'C
Cuts at: 4589 4589
Size: 7264
MfeI C'AATT_G
Cuts at: 4863 4863
Size: 7264
MluI A'CGCG_T
Cuts at: 7140 7140
Size: 7264
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2124 2982 3823 4485 4546 6378 6562 1305
Size: 819 858 841 662 61 1832 184 2007
Fragments arranged by size:
2007 1832 858 841 819 662 184 61
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2873 10
Size: 54 1425 343 1041 4401
Fragments arranged by size:
4401 1425 1041 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2028 2732 4454 5222 5581 5740 518
Size: 648 862 704 1722 768 359 159 2042
Fragments arranged by size:
2042 1722 862 768 704 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2309 2989 3155 3542 4680 5357 6505 6750
Size: 703 680 166 387 1138 677 1148 245
Cuts at: 6750 1606
Size: 2120
Fragments arranged by size:
2120 1148 1138 703 680 677 387 245
166
NaeI GCC'GGC
Cuts at: 2047 2384 4013 2047
Size: 337 1629 5298
Fragments arranged by size:
5298 1629 337
NarI GG'CG_CC
Cuts at: 3616 3616
Size: 7264
NcoI C'CATG_G
Cuts at: 513 1065 2474 2727 513
Size: 552 1409 253 5050
Fragments arranged by size:
5050 1409 552 253
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7264
NgoMIV G'CCGG_C
Cuts at: 2045 2382 4011 2045
Size: 337 1629 5298
Fragments arranged by size:
5298 1629 337
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7264
NotI GC'GGCC_GC
Cuts at: 4711 4711
Size: 7264
NruI TCG'CGA
Cuts at: 2164 3758 2164
Size: 1594 5670
NspI r_CATG'y
Cuts at: 1132 1229 2779 3378 3409 3583 4560 4622 6167
Size: 97 1550 599 31 174 977 62 1545
Cuts at: 6167 1132
Size: 2229
Fragments arranged by size:
2229 1550 1545 977 599 174 97 62
31
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7264
PciI A'CATG_T
Cuts at: 3405 3579 6163 3405
Size: 174 2584 4506
Fragments arranged by size:
4506 2584 174
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2920 1108
Size: 1812 5452
PfoI T'CCnGG_A
Cuts at: 2115 2277 4143 4502 2115
Size: 162 1866 359 4877
Fragments arranged by size:
4877 1866 359 162
PmeI GTTT'AAAC
Cuts at: 4568 4568
Size: 7264
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5333 5365 6846 6878 5333
Size: 32 1481 32 5719
Fragments arranged by size:
5719 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2436 2683 4500 2436
Size: 247 1817 5200
Fragments arranged by size:
5200 1817 247
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7264
PsiI TTA'TAA
Cuts at: 4834 4834
Size: 7264
PspOMI G'GGCC_C
Cuts at: 3009 3922 4197 3009
Size: 913 275 6076
Fragments arranged by size:
6076 913 275
PspXI vC'TCGA_Gb
Cuts at: 4265 4265
Size: 7264
PstI C_TGCA'G
Cuts at: 830 2087 2827 3645 4275 4616 830
Size: 1257 740 818 630 341 3478
Fragments arranged by size:
3478 1257 818 740 630 341
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7264
PvuII CAG'CTG
Cuts at: 2309 2989 3155 2309
Size: 680 166 6418
Fragments arranged by size:
6418 680 166
SacI G_AGCT'C
Cuts at: 4419 4419
Size: 7264
SalI G'TCGA_C
Cuts at: 1936 4606 1936
Size: 2670 4594
SapI GCTCTTCn'nnn_
Cuts at: 2279 4022 2279
Size: 1743 5521
SbfI CC_TGCA'GG
Cuts at: 3645 4616 3645
Size: 971 6293
ScaI AGT'ACT
Cuts at: 5512 7224 5512
Size: 1712 5552
SexAI A'CCwGG_T
Cuts at: 2432 2432
Size: 7264
SfcI C'TryA_G
Cuts at: 826 1045 2083 2823 3641 4271 4612 5747 6428
Size: 219 1038 740 818 630 341 1135 681
Cuts at: 6428 6619 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1038 818 740 681 630 341
219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2388 2388
Size: 7264
SfoI GGC'GCC
Cuts at: 3617 3617
Size: 7264
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7264
SgrAI Cr'CCGG_yG
Cuts at: 1700 2045 1700
Size: 345 6919
SmaI CCC'GGG
Cuts at: 1872 4225 4591 1872
Size: 2353 366 4545
Fragments arranged by size:
4545 2353 366
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3200 3392 4265 5368 6269 6531
Size: 197 926 1257 192 873 1103 901 262
Cuts at: 6531 820
Size: 1553
Fragments arranged by size:
1553 1257 1103 926 901 873 262 197
192
SnaBI TAC'GTA
Cuts at: 493 5055 493
Size: 4562 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7264
SphI G_CATG'C
Cuts at: 1132 2779 4622 1132
Size: 1647 1843 3774
Fragments arranged by size:
3774 1843 1647
SspI AAT'ATT
Cuts at: 5 52 3910 5188 5
Size: 47 3858 1278 2081
Fragments arranged by size:
3858 2081 1278 47
StuI AGG'CCT
Cuts at: 1517 2359 3647 3993 1517
Size: 842 1288 346 4788
Fragments arranged by size:
4788 1288 842 346
StyI C'CwwG_G
Cuts at: 513 1065 2474 2727 4056 4703 513
Size: 552 1409 253 1329 647 3074
Fragments arranged by size:
3074 1409 1329 647 552 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3205 3246 5291 5308 5461 5646 6091
Size: 1255 1625 41 2045 17 153 185 445
Cuts at: 6091 325
Size: 1498
Fragments arranged by size:
2045 1625 1498 1255 445 185 153 41
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4315 5510 7222
Size: 274 80 33 51 1443 2338 1195 1712
Cuts at: 7222 96
Size: 138
Fragments arranged by size:
2338 1712 1443 1195 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2345 2377 2675 2707 7224 7256 2345
Size: 32 298 32 4517 32 2353
Fragments arranged by size:
4517 2353 298 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7264
XbaI T'CTAG_A
Cuts at: 4600 4600
Size: 7264
XhoI C'TCGA_G
Cuts at: 1943 4265 1943
Size: 2322 4942
XmaI C'CCGG_G
Cuts at: 1870 4223 4589 1870
Size: 2353 366 4545
Fragments arranged by size:
4545 2353 366
XmnI GAAnn'nnTTC
Cuts at: 5393 5393
Size: 7264
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5072 276
Size: 53 83 186 936 3538 2468
Fragments arranged by size:
3538 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NsiI PacI
PmlI PsrI RsrII SacII SanDI SrfI SwaI XcmI