Restriction map for
pFN21AE0346
(Circular) (Six-base) MAPSORT of: pFN21AE0346.seq Check: 4672 from: 1 to: 6610
pFN21AE0346 6610 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2476 2476
Size: 6610
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2099 2763 4420 278
Size: 53 83 186 936 563 664 1657 2468
Fragments arranged by size:
2468 1657 936 664 563 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3953 1937
Size: 2016 4594
Acc65I G'GTAC_C
Cuts at: 3931 3931
Size: 6610
AclI AA'CG_TT
Cuts at: 1563 4737 5110 1563
Size: 3174 373 3063
Fragments arranged by size:
3174 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2087 3398 4673 6057 6325 1632
Size: 455 1311 1275 1384 268 1917
Fragments arranged by size:
1917 1384 1311 1275 455 268
AfeI AGC'GCT
Cuts at: 2674 2916 2674
Size: 242 6368
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6413
AflIII A'CryG_T
Cuts at: 1203 2287 5509 6486 1203
Size: 1084 3222 977 1327
Fragments arranged by size:
3222 1327 1084 977
AgeI A'CCGG_T
Cuts at: 5441 5441
Size: 6610
AhdI GACnn_n'nnGTC
Cuts at: 3166 5339 3166
Size: 2173 4437
AleI CACnn'nnGTG
Cuts at: 1166 3215 1166
Size: 2049 4561
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2062 2281 1184
Size: 878 219 5513
Fragments arranged by size:
5513 878 219
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2420 2452 2420
Size: 32 6578
AlwNI CAG_nnn'CTG
Cuts at: 2056 3184 4353 5925 2056
Size: 1128 1169 1572 2741
Fragments arranged by size:
2741 1572 1169 1128
ApaLI G'TGCA_C
Cuts at: 3083 4667 5823 3083
Size: 1584 1156 3870
Fragments arranged by size:
3870 1584 1156
ApoI r'AATT_y
Cuts at: 1493 2276 3919 4145 4297 6406 1493
Size: 783 1643 226 152 2109 1697
Fragments arranged by size:
2109 1697 1643 783 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5164 160
Size: 872 4132 1606
Fragments arranged by size:
4132 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6610
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 2161 3935 1870
Size: 73 67 151 1774 4545
Fragments arranged by size:
4545 1774 151 73 67
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2310 2343 2310
Size: 33 6577
BamHI G'GATC_C
Cuts at: 3940 4307 3940
Size: 367 6243
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2183 2393 2488 2846 2884
Size: 325 217 627 396 210 95 358 38
Cuts at: 2884 3100 3113 3880 3931 5386 618
Size: 216 13 767 51 1455 1842
Fragments arranged by size:
1842 1455 767 627 396 358 325 217
216 210 95 51 38 13
BanII G_rGCy'C
Cuts at: 1391 2162 2306 2569 3060 3132 3314 3595 3929
Size: 771 144 263 491 72 182 281 334
Cuts at: 3929 1391
Size: 4072
Fragments arranged by size:
4072 771 491 334 281 263 182 144
72
BbeI G_GCGC'C
Cuts at: 2397 2397
Size: 6610
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3246 3621 928
Size: 971 1347 375 3917
Fragments arranged by size:
3917 1347 971 375
BbvCI CC'TCA_GC
Cuts at: 3466 3466
Size: 6610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2747 2781 3590 3624 4801 4835 1903
Size: 34 810 34 809 34 1177 34 3678
Fragments arranged by size:
3678 1177 810 809 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2338 4502 5712 2338
Size: 2164 1210 3236
Fragments arranged by size:
3236 2164 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6610
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2243 2402 3146
Size: 107 122 71 631 605 571 159 744
Cuts at: 3146 5221 136
Size: 2075 1525
Fragments arranged by size:
2075 1525 744 631 605 571 159 122
107 71
BglII A'GATC_T
Cuts at: 6605 6605
Size: 6610
BlpI GC'TnA_GC
Cuts at: 3005 3572 4027 3005
Size: 567 455 5588
Fragments arranged by size:
5588 567 455
Bme1580I G_kGCm'C
Cuts at: 1790 2287 2493 2889 3087 3116 3344 4671 5827
Size: 497 206 396 198 29 228 1327 1156
Cuts at: 5827 1790
Size: 2573
Fragments arranged by size:
2573 1327 1156 497 396 228 206 198
29
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6610
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3535 4341 5299
Size: 416 53 83 399 24 2118 806 958
Cuts at: 5299 442
Size: 1753
Fragments arranged by size:
2118 1753 958 806 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2034 1056
Size: 978 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2434 2689 2758 3185 3447 3863 5270
Size: 857 338 255 69 427 262 416 1407
Cuts at: 5270 1239
Size: 2579
Fragments arranged by size:
2579 1407 857 427 416 338 262 255
69
Bpu10I CC'TnA_GC
Cuts at: 3363 3466 3656 3363
Size: 103 190 6317
Fragments arranged by size:
6317 190 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4735 5600 5898 4735
Size: 865 298 5447
Fragments arranged by size:
5447 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2171 2459 5273 882
Size: 617 672 288 2814 2219
Fragments arranged by size:
2814 2219 672 617 288
BsaAI yAC'GTr
Cuts at: 493 1204 2173 2288 2326 2473 3820 4401 493
Size: 711 969 115 38 147 1347 581 2702
Fragments arranged by size:
2702 1347 969 711 581 147 115 38
BsaBI GATnn'nnATC
Cuts at: 6604 6604
Size: 6610
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2096 2394 2712 2760
Size: 53 83 186 936 563 298 318 48
Cuts at: 2760 4417 4799 5434 275
Size: 1657 382 635 1451
Fragments arranged by size:
1657 1451 936 635 563 382 318 298
186 83 53 48
BsaWI w'CCGG_w
Cuts at: 5043 5441 5715 5862 6273 5043
Size: 398 274 147 411 5380
Fragments arranged by size:
5380 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2556 2586 3420 3450 1178
Size: 30 165 30 1153 30 834 30 4338
Fragments arranged by size:
4338 1153 834 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2363 2795 3002 3122 3860 1186
Size: 1177 432 207 120 738 3936
Fragments arranged by size:
3936 1177 738 432 207 120
BseYI C'CCAG_C
Cuts at: 2655 3263 3277 3872 5813 6309 2655
Size: 608 14 595 1941 496 2956
Fragments arranged by size:
2956 1941 608 595 496 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3497 3875 1163
Size: 573 1761 378 3898
Fragments arranged by size:
3898 1761 573 378
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4060 4821 5849 1540
Size: 275 186 2059 761 1028 2301
Fragments arranged by size:
2301 2059 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2178 2220 3087 3314 4671 4756 5827 2178
Size: 42 867 227 1357 85 1071 2961
Fragments arranged by size:
2961 1357 1071 867 227 85 42
BsmI GAATG_Cn'
Cuts at: 4121 4214 4121
Size: 93 6517
BsmBI CGTCTCn'nnnn_
Cuts at: 2087 2087
Size: 6610
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2162 2178 2220 2287 2306 2493 2569
Size: 399 372 16 42 67 19 187 76
Cuts at: 2569 2889 3060 3087 3116 3132 3314 3344 3595
Size: 320 171 27 29 16 182 30 251
Cuts at: 3595 3929 4671 4756 5827 1391
Size: 334 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 372 334 320 251
187 182 171 85 76 67 42 30
29 27 19 16 16
BspEI T'CCGG_A
Cuts at: 6273 6273
Size: 6610
BspHI T'CATG_A
Cuts at: 4499 6229 4499
Size: 1730 4880
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2476 3965 844
Size: 1632 1489 3489
Fragments arranged by size:
3489 1632 1489
BsrBI CCG'CTC
Cuts at: 1394 1823 4497 1394
Size: 429 2674 3507
Fragments arranged by size:
3507 2674 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2025 2763 2835 5105 5279 65
Size: 1183 777 738 72 2270 174 1396
Fragments arranged by size:
2270 1396 1183 777 738 174 72
BsrFI r'CCGG_y
Cuts at: 1700 1810 2720 5254 5441 1700
Size: 110 910 2534 187 2869
Fragments arranged by size:
2869 2534 910 187 110
BsrGI T'GTAC_A
Cuts at: 96 3346 96
Size: 3250 3360
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6610
BssSI C'ACGA_G
Cuts at: 4670 5682 4670
Size: 1012 5598
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2846 3862 794
Size: 2052 1016 3542
Fragments arranged by size:
3542 2052 1016
BstBI TT'CG_AA
Cuts at: 5414 5414
Size: 6610
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6610
BstXI CCAn_nnnn'nTGG
Cuts at: 2200 2200
Size: 6610
BstYI r'GATC_y
Cuts at: 1972 3627 3940 4307 4692 4709 6161 6247 6259
Size: 1655 313 367 385 17 1452 86 12
Cuts at: 6259 6605 1972
Size: 346 1977
Fragments arranged by size:
1977 1655 1452 385 367 346 313 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3389 3389
Size: 6610
BtgI C'CryG_G
Cuts at: 513 1065 2004 2187 2331 2400 2795 3042 3633
Size: 552 939 183 144 69 395 247 591
Cuts at: 3633 513
Size: 3490
Fragments arranged by size:
3490 939 591 552 395 247 183 144
69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2803 487
Size: 683 56 1577 4294
Fragments arranged by size:
4294 1577 683 56
BtsI GCAGTG_nn'
Cuts at: 2099 2724 3149 3438 3750 4126 4920 4940 2099
Size: 625 425 289 312 376 794 20 3769
Fragments arranged by size:
3769 794 625 425 376 312 289 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6610
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6575
DraI TTT'AAA
Cuts at: 3914 3914
Size: 6610
DraIII CAC_nnn'GTG
Cuts at: 3820 3820
Size: 6610
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5617 809
Size: 507 4301 1802
Fragments arranged by size:
4301 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2190 2235 2601
Size: 54 1425 50 275 18 360 45 366
Cuts at: 2601 2718 2814 3045 3219 3898 4057 4946 6575
Size: 117 96 231 174 679 159 889 1629
Cuts at: 6575 8
Size: 43
Fragments arranged by size:
1629 1425 889 679 366 360 275 231
174 159 117 96 54 50 45 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4057 1537
Size: 275 2245 4090
Fragments arranged by size:
4090 2245 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2139 2630 3020 3065 4540 1353
Size: 786 491 390 45 1475 3423
Fragments arranged by size:
3423 1475 786 491 390 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5197 5571 5717 387
Size: 1064 138 3608 374 146 1280
Fragments arranged by size:
3608 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3312 3312
Size: 6610
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2096 2434 2689 2758 3185 3398
Size: 393 455 9 338 255 69 427 213
Cuts at: 3398 3447 3863 4673 5270 6057 6325 1239
Size: 49 416 810 597 787 268 1524
Fragments arranged by size:
1524 810 787 597 455 427 416 393
338 268 255 213 69 49 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3366 3366
Size: 6610
EcoO109I rG'GnC_Cy
Cuts at: 3393 3892 3393
Size: 499 6111
EcoRI G'AATT_C
Cuts at: 3919 4297 3919
Size: 378 6232
FspI TGC'GCA
Cuts at: 5116 5116
Size: 6610
HaeII r_GCGC'y
Cuts at: 1678 2110 2397 2676 2918 3449 5757 1678
Size: 432 287 279 242 531 2308 2531
Fragments arranged by size:
2531 2308 531 432 287 279 242
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2894 2926 3152 3184 5251
Size: 32 135 32 1322 32 226 32 2067
Cuts at: 5251 5283 5325 5357 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2067 1322 226 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3954 4200 669
Size: 988 281 2016 246 3079
Fragments arranged by size:
3079 2016 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6610
HpaI GTT'AAC
Cuts at: 4200 4200
Size: 6610
KasI G'GCGC_C
Cuts at: 2393 2393
Size: 6610
KpnI G_GTAC'C
Cuts at: 3935 3935
Size: 6610
MfeI C'AATT_G
Cuts at: 4209 4209
Size: 6610
MluI A'CGCG_T
Cuts at: 6486 6486
Size: 6610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5724 5908 1305
Size: 4419 184 2007
Fragments arranged by size:
4419 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2192 2816 3047 3221 3900
Size: 54 1425 343 360 624 231 174 679
Cuts at: 3900 10
Size: 2720
Fragments arranged by size:
2720 1425 679 624 360 343 231 174
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2044 3215 4568 4927 5086 518
Size: 648 878 1171 1353 359 159 2042
Fragments arranged by size:
2042 1353 1171 878 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2074 2240 2843 2938 2972 3334 3787 4026
Size: 468 166 603 95 34 362 453 239
Cuts at: 4026 4703 5851 6096 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 603 468 453 362 245
239 166 95 34
NaeI GCC'GGC
Cuts at: 2722 2722
Size: 6610
NarI GG'CG_CC
Cuts at: 2394 2394
Size: 6610
NcoI C'CATG_G
Cuts at: 513 1065 2004 2331 3042 3633 513
Size: 552 939 327 711 591 3490
Fragments arranged by size:
3490 939 711 591 552 327
NdeI CA'TA_TG
Cuts at: 387 3903 387
Size: 3516 3094
NgoMIV G'CCGG_C
Cuts at: 2720 2720
Size: 6610
NheI G'CTAG_C
Cuts at: 1052 2030 1052
Size: 978 5632
NotI GC'GGCC_GC
Cuts at: 4057 4057
Size: 6610
NspI r_CATG'y
Cuts at: 1132 1229 3968 5513 1132
Size: 97 2739 1545 2229
Fragments arranged by size:
2739 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3831 1348
Size: 2483 4127
PciI A'CATG_T
Cuts at: 5509 5509
Size: 6610
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6610
PmeI GTTT'AAAC
Cuts at: 3914 3914
Size: 6610
PmlI CAC'GTG
Cuts at: 2288 2473 2288
Size: 185 6425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4679 4711 6192 6224 4679
Size: 32 1481 32 5065
Fragments arranged by size:
5065 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3892 3892
Size: 6610
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6610
PsiI TTA'TAA
Cuts at: 4180 4180
Size: 6610
PstI C_TGCA'G
Cuts at: 830 3962 830
Size: 3132 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6610
PvuII CAG'CTG
Cuts at: 2843 2972 3334 2843
Size: 129 362 6119
Fragments arranged by size:
6119 362 129
SacI G_AGCT'C
Cuts at: 3314 3314
Size: 6610
SalI G'TCGA_C
Cuts at: 1936 3952 1936
Size: 2016 4594
SapI GCTCTTCn'nnn_
Cuts at: 3065 3065
Size: 6610
SbfI CC_TGCA'GG
Cuts at: 3962 3962
Size: 6610
ScaI AGT'ACT
Cuts at: 4858 6570 4858
Size: 1712 4898
SfcI C'TryA_G
Cuts at: 826 1045 2944 3329 3958 5093 5774 5965 826
Size: 219 1899 385 629 1135 681 191 1471
Fragments arranged by size:
1899 1471 1135 681 629 385 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2243 2243
Size: 6610
SfoI GGC'GCC
Cuts at: 2395 2395
Size: 6610
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6610
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6610
SmaI CCC'GGG
Cuts at: 1872 2012 3937 1872
Size: 140 1925 4545
Fragments arranged by size:
4545 1925 140
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4714 5615 5877 820
Size: 197 926 2771 901 262 1553
Fragments arranged by size:
2771 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2326 4401 493
Size: 1833 2075 2702
Fragments arranged by size:
2702 2075 1833
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6610
SphI G_CATG'C
Cuts at: 1132 3968 1132
Size: 2836 3774
SspI AAT'ATT
Cuts at: 5 52 4534 5
Size: 47 4482 2081
Fragments arranged by size:
4482 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6610
StyI C'CwwG_G
Cuts at: 513 1065 2004 2193 2331 3042 3190 3633 4049
Size: 552 939 189 138 711 148 443 416
Cuts at: 4049 513
Size: 3074
Fragments arranged by size:
3074 939 711 552 443 416 189 148
138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2135 3697 4637 4654 4807 4992 5437
Size: 1255 555 1562 940 17 153 185 445
Cuts at: 5437 325
Size: 1498
Fragments arranged by size:
1562 1498 1255 940 555 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3346 4856 6568
Size: 274 80 33 51 1443 1369 1510 1712
Cuts at: 6568 96
Size: 138
Fragments arranged by size:
1712 1510 1443 1369 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2556 2588 3420 3452 3729 3761 6570 6602 2556
Size: 32 832 32 277 32 2809 32 2564
Fragments arranged by size:
2809 2564 832 277 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6610
XbaI T'CTAG_A
Cuts at: 3946 3946
Size: 6610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2032 3817 3838 2032
Size: 1785 21 4804
Fragments arranged by size:
4804 1785 21
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6610
XmaI C'CCGG_G
Cuts at: 1870 2010 3935 1870
Size: 140 1925 4545
Fragments arranged by size:
4545 1925 140
XmnI GAAnn'nnTTC
Cuts at: 4739 4739
Size: 6610
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2097 2761 4418 276
Size: 53 83 186 936 563 664 1657 2468
Fragments arranged by size:
2468 1657 936 664 563 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BfrBI BplI BsiWI BstZ17I EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI