Restriction map for
pFN21AE0345
(Circular) (Six-base) MAPSORT of: pFN21AE0345.seq Check: 1688 from: 1 to: 6412
pFN21AE0345 6412 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3328 3328
Size: 6412
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4222 278
Size: 53 83 186 936 2686 2468
Fragments arranged by size:
2686 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3755 1937
Size: 1818 4594
Acc65I G'GTAC_C
Cuts at: 3307 3733 3307
Size: 426 5986
AclI AA'CG_TT
Cuts at: 1563 4539 4912 1563
Size: 2976 373 3063
Fragments arranged by size:
3063 2976 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3259 4475 5859 6127 1632
Size: 1627 1216 1384 268 1917
Fragments arranged by size:
1917 1627 1384 1216 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6215
AflIII A'CryG_T
Cuts at: 1203 5311 6288 1203
Size: 4108 977 1327
Fragments arranged by size:
4108 1327 977
AgeI A'CCGG_T
Cuts at: 5243 5243
Size: 6412
AhdI GACnn_n'nnGTC
Cuts at: 5141 5141
Size: 6412
AleI CACnn'nnGTG
Cuts at: 1166 3545 1166
Size: 2379 4033
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6412
AlwNI CAG_nnn'CTG
Cuts at: 3529 3575 4155 5727 3529
Size: 46 580 1572 4214
Fragments arranged by size:
4214 1572 580 46
ApaI G_GGCC'C
Cuts at: 2856 3073 2856
Size: 217 6195
ApaLI G'TGCA_C
Cuts at: 4469 5625 4469
Size: 1156 5256
ApoI r'AATT_y
Cuts at: 1493 3721 3947 4099 6208 1493
Size: 2228 226 152 2109 1697
Fragments arranged by size:
2228 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4966 160
Size: 872 3934 1606
Fragments arranged by size:
3934 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6412
AvaI C'yCGr_G
Cuts at: 1870 1943 2901 2921 3116 3737 1870
Size: 73 958 20 195 621 4545
Fragments arranged by size:
4545 958 621 195 73 20
BamHI G'GATC_C
Cuts at: 3246 3742 4109 3246
Size: 496 367 5549
Fragments arranged by size:
5549 496 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2404 2675 3307 3594 3733
Size: 325 217 627 617 271 632 287 139
Cuts at: 3733 5188 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 632 627 617 325 287 271
217 139
BanII G_rGCy'C
Cuts at: 1391 2235 2467 2856 2921 3073 3510 3731 1391
Size: 844 232 389 65 152 437 221 4072
Fragments arranged by size:
4072 844 437 389 232 221 152 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5441
BbvCI CC'TCA_GC
Cuts at: 2086 2307 2754 2086
Size: 221 447 5744
Fragments arranged by size:
5744 447 221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2239 2273 3657 3691 4603 4637 1903
Size: 34 302 34 1384 34 912 34 3678
Fragments arranged by size:
3678 1384 912 302 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4304 5514 4304
Size: 1210 5202
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6412
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3390 5023 136
Size: 107 122 71 631 605 1718 1633 1525
Fragments arranged by size:
1718 1633 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3075 6407 3075
Size: 3332 3080
BlpI GC'TnA_GC
Cuts at: 2778 2846 3829 2778
Size: 68 983 5361
Fragments arranged by size:
5361 983 68
Bme1580I G_kGCm'C
Cuts at: 1790 2680 2856 3073 3146 3368 3428 4473 5629
Size: 890 176 217 73 222 60 1045 1156
Cuts at: 5629 1790
Size: 2573
Fragments arranged by size:
2573 1156 1045 890 222 217 176 73
60
BmgBI CAC'GTC
Cuts at: 1318 2818 3487 1318
Size: 1500 669 4243
Fragments arranged by size:
4243 1500 669
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2829 4143 5101
Size: 416 53 83 399 24 1412 1314 958
Cuts at: 5101 442
Size: 1753
Fragments arranged by size:
1753 1412 1314 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3605 3667 5072 1239
Size: 2366 62 1405 2579
Fragments arranged by size:
2579 2366 1405 62
Bpu10I CC'TnA_GC
Cuts at: 2086 2307 2754 2086
Size: 221 447 5744
Fragments arranged by size:
5744 447 221
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4537 5402 5700 4537
Size: 865 298 5249
Fragments arranged by size:
5249 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3581 5075 882
Size: 617 2082 1494 2219
Fragments arranged by size:
2219 2082 1494 617
BsaAI yAC'GTr
Cuts at: 493 1204 4203 493
Size: 711 2999 2702
Fragments arranged by size:
2999 2702 711
BsaBI GATnn'nnATC
Cuts at: 6406 6406
Size: 6412
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4219 4601 5236 275
Size: 53 83 186 936 2686 382 635 1451
Fragments arranged by size:
2686 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2964 3399 4845 5243 5517 5664 6075 2964
Size: 435 1446 398 274 147 411 3301
Fragments arranged by size:
3301 1446 435 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2805 2835 2882 2912 3026
Size: 30 165 30 1402 30 47 30 114
Cuts at: 3026 3056 3086 3116 3203 3233 1178
Size: 30 30 30 87 30 4357
Fragments arranged by size:
4357 1402 165 114 87 47 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3518 1186
Size: 2332 4080
BseYI C'CCAG_C
Cuts at: 2026 2480 3060 3284 3419 3663 5615 6111 2026
Size: 454 580 224 135 244 1952 496 2327
Fragments arranged by size:
2327 1952 580 496 454 244 224 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3432 3500 1163
Size: 573 1696 68 4075
Fragments arranged by size:
4075 1696 573 68
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2736 3862 4623 5651 1540
Size: 275 186 735 1126 761 1028 2301
Fragments arranged by size:
2301 1126 1028 761 735 275 186
BsiHKAI G_wGCw'C
Cuts at: 2156 2793 4473 4558 5629 2156
Size: 637 1680 85 1071 2939
Fragments arranged by size:
2939 1680 1071 637 85
BsmI GAATG_Cn'
Cuts at: 2245 3923 4016 2245
Size: 1678 93 4641
Fragments arranged by size:
4641 1678 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2651 2808 2651
Size: 157 6255
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2156 2235 2467 2680 2793 2856 2921
Size: 399 366 79 232 213 113 63 65
Cuts at: 2921 3073 3146 3368 3428 3510 3731 4473 4558
Size: 152 73 222 60 82 221 742 85
Cuts at: 4558 5629 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 366 232 222 221
213 152 113 85 82 79 73 65
63 60
BspEI T'CCGG_A
Cuts at: 3399 6075 3399
Size: 2676 3736
BspHI T'CATG_A
Cuts at: 2235 4301 6031 2235
Size: 2066 1730 2616
Fragments arranged by size:
2616 2066 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3328 3767 844
Size: 2484 439 3489
Fragments arranged by size:
3489 2484 439
BsrBI CCG'CTC
Cuts at: 1394 1823 2845 3231 3352 4299 1394
Size: 429 1022 386 121 947 3507
Fragments arranged by size:
3507 1022 947 429 386 121
BsrDI GCAATG_nn'
Cuts at: 65 1248 2208 4907 5081 65
Size: 1183 960 2699 174 1396
Fragments arranged by size:
2699 1396 1183 960 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3704 5056 5243 1700
Size: 110 1894 1352 187 2869
Fragments arranged by size:
2869 1894 1352 187 110
BsrGI T'GTAC_A
Cuts at: 96 2115 2826 96
Size: 2019 711 3682
Fragments arranged by size:
3682 2019 711
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6412
BssSI C'ACGA_G
Cuts at: 4472 5484 4472
Size: 1012 5400
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2210 3575 794
Size: 1416 1365 3631
Fragments arranged by size:
3631 1416 1365
BstBI TT'CG_AA
Cuts at: 5216 5216
Size: 6412
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6412
BstXI CCAn_nnnn'nTGG
Cuts at: 2447 3010 3451 2447
Size: 563 441 5408
Fragments arranged by size:
5408 563 441
BstYI r'GATC_y
Cuts at: 1972 2415 3075 3246 3742 4109 4494 4511 5963
Size: 443 660 171 496 367 385 17 1452
Cuts at: 5963 6049 6061 6407 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 660 496 443 385 367 346
171 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2040 2130 2136 2511 3438 3498 513
Size: 552 975 90 6 375 927 60 3427
Fragments arranged by size:
3427 975 927 552 375 90 60 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3610 487
Size: 683 56 2384 3289
Fragments arranged by size:
3289 2384 683 56
BtsI GCAGTG_nn'
Cuts at: 2156 2174 3144 3184 3444 3576 3928 4722 4742
Size: 18 970 40 260 132 352 794 20
Cuts at: 4742 2156
Size: 3826
Fragments arranged by size:
3826 970 794 352 260 132 40 20
18
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6412
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6377
DraI TTT'AAA
Cuts at: 3716 3716
Size: 6412
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3395 5419 809
Size: 507 2079 2024 1802
Fragments arranged by size:
2079 2024 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2133 2664
Size: 54 1425 50 275 18 207 96 531
Cuts at: 2664 2733 3391 3441 3453 3859 4748 6377 8
Size: 69 658 50 12 406 889 1629 43
Fragments arranged by size:
1629 1425 889 658 531 406 275 207
96 69 54 50 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2733 3859 1537
Size: 275 921 1126 4090
Fragments arranged by size:
4090 1126 921 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2276 2477 4342 1353
Size: 923 201 1865 3423
Fragments arranged by size:
3423 1865 923 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3178 4999 5373 5519 387
Size: 1064 138 1589 1821 374 146 1280
Fragments arranged by size:
1821 1589 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3259 3605 3667 4475 5072 5859 6127
Size: 393 1627 346 62 808 597 787 268
Cuts at: 6127 1239
Size: 1524
Fragments arranged by size:
1627 1524 808 787 597 393 346 268
62
EcoO109I rG'GnC_Cy
Cuts at: 2758 2932 2985 2998 2758
Size: 174 53 13 6172
Fragments arranged by size:
6172 174 53 13
EcoRI G'AATT_C
Cuts at: 3721 4099 3721
Size: 378 6034
FspI TGC'GCA
Cuts at: 4918 4918
Size: 6412
HaeII r_GCGC'y
Cuts at: 1678 5559 1678
Size: 3881 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2174 2206 3263 3295 5053
Size: 32 135 32 602 32 1057 32 1758
Cuts at: 5053 5085 5127 5159 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1758 1057 602 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3756 4002 669
Size: 988 281 1818 246 3079
Fragments arranged by size:
3079 1818 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6412
HpaI GTT'AAC
Cuts at: 4002 4002
Size: 6412
KpnI G_GTAC'C
Cuts at: 3311 3737 3311
Size: 426 5986
MfeI C'AATT_G
Cuts at: 4011 4011
Size: 6412
MluI A'CGCG_T
Cuts at: 6288 6288
Size: 6412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5526 5710 1305
Size: 4221 184 2007
Fragments arranged by size:
4221 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2666 3455 10
Size: 54 1425 343 834 789 2967
Fragments arranged by size:
2967 1425 834 789 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3545 3629 4370 4729 4888 518
Size: 648 2379 84 741 359 159 2042
Fragments arranged by size:
2379 2042 741 648 359 159 84
MspA1I CmG'CkG
Cuts at: 1606 2138 2443 2491 2641 2753 2797 2850 3243
Size: 532 305 48 150 112 44 53 393
Cuts at: 3243 3671 3828 4505 5653 5898 1606
Size: 428 157 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 532 428 393 305 245
157 150 112 53 48 44
NaeI GCC'GGC
Cuts at: 3706 3706
Size: 6412
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5860
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6412
NgoMIV G'CCGG_C
Cuts at: 3704 3704
Size: 6412
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6412
NotI GC'GGCC_GC
Cuts at: 3859 3859
Size: 6412
NspI r_CATG'y
Cuts at: 1132 1229 2833 3770 5315 1132
Size: 97 1604 937 1545 2229
Fragments arranged by size:
2229 1604 1545 937 97
PasI CC'CwG_GG
Cuts at: 1348 2460 1348
Size: 1112 5300
PciI A'CATG_T
Cuts at: 5311 5311
Size: 6412
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2251 1108
Size: 1143 5269
PfoI T'CCnGG_A
Cuts at: 3683 3683
Size: 6412
PmeI GTTT'AAAC
Cuts at: 3716 3716
Size: 6412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4481 4513 5994 6026 4481
Size: 32 1481 32 4867
Fragments arranged by size:
4867 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2758 2985 2998 2758
Size: 227 13 6172
Fragments arranged by size:
6172 227 13
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6412
PsiI TTA'TAA
Cuts at: 3982 3982
Size: 6412
PspOMI G'GGCC_C
Cuts at: 2852 3069 2852
Size: 217 6195
PstI C_TGCA'G
Cuts at: 830 3580 3764 830
Size: 2750 184 3478
Fragments arranged by size:
3478 2750 184
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6412
PvuII CAG'CTG
Cuts at: 2491 2641 2753 3671 2491
Size: 150 112 918 5232
Fragments arranged by size:
5232 918 150 112
RsrII CG'GwC_CG
Cuts at: 2550 2550
Size: 6412
SacII CC_GC'GG
Cuts at: 2139 2139
Size: 6412
SalI G'TCGA_C
Cuts at: 1936 3754 1936
Size: 1818 4594
SanDI GG'GwC_CC
Cuts at: 2998 2998
Size: 6412
SbfI CC_TGCA'GG
Cuts at: 3764 3764
Size: 6412
ScaI AGT'ACT
Cuts at: 4660 6372 4660
Size: 1712 4700
SfcI C'TryA_G
Cuts at: 826 1045 3576 3760 4895 5576 5767 826
Size: 219 2531 184 1135 681 191 1471
Fragments arranged by size:
2531 1471 1135 681 219 191 184
SfiI GGCCn_nnn'nGGCC
Cuts at: 3390 3390
Size: 6412
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6412
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6412
SmaI CCC'GGG
Cuts at: 1872 2903 3739 1872
Size: 1031 836 4545
Fragments arranged by size:
4545 1031 836
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4516 5417 5679 820
Size: 197 926 2573 901 262 1553
Fragments arranged by size:
2573 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4203 493
Size: 3710 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6412
SphI G_CATG'C
Cuts at: 1132 3770 1132
Size: 2638 3774
SspI AAT'ATT
Cuts at: 5 52 4336 5
Size: 47 4284 2081
Fragments arranged by size:
4284 2081 47
StuI AGG'CCT
Cuts at: 1517 2684 1517
Size: 1167 5245
StyI C'CwwG_G
Cuts at: 513 1065 2094 3222 3851 513
Size: 552 1029 1128 629 3074
Fragments arranged by size:
3074 1128 1029 629 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3620 4439 4456 4609 4794 5239 325
Size: 1255 2040 819 17 153 185 445 1498
Fragments arranged by size:
2040 1498 1255 819 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2115 2826 4658
Size: 274 80 33 51 1443 138 711 1832
Cuts at: 4658 6370 96
Size: 1712 138
Fragments arranged by size:
1832 1712 1443 711 274 138 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2805 2837 3086 3118 6372 6404 2805
Size: 32 249 32 3254 32 2813
Fragments arranged by size:
3254 2813 249 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6412
XbaI T'CTAG_A
Cuts at: 3748 3748
Size: 6412
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2128 2488 2128
Size: 360 6052
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6412
XmaI C'CCGG_G
Cuts at: 1870 2901 3737 1870
Size: 1031 836 4545
Fragments arranged by size:
4545 1031 836
XmnI GAAnn'nnTTC
Cuts at: 4541 4541
Size: 6412
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4220 276
Size: 53 83 186 936 2686 2468
Fragments arranged by size:
2686 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacII SalI SanDI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI SacI SapI SexAI SfoI SrfI SwaI