Restriction map for
pFN21AE0342
(Circular) (Six-base) MAPSORT of: pFN21AE0342.seq Check: 8174 from: 1 to: 6580
pFN21AE0342 6580 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2572 2854 2572
Size: 282 6298
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2271 4390 278
Size: 53 83 186 936 735 2119 2468
Fragments arranged by size:
2468 2119 936 735 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3923 1937
Size: 1986 4594
Acc65I G'GTAC_C
Cuts at: 3901 3901
Size: 6580
AclI AA'CG_TT
Cuts at: 1563 4707 5080 1563
Size: 3144 373 3063
Fragments arranged by size:
3144 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2245 3059 4643 6027 6295 1632
Size: 613 814 1584 1384 268 1917
Fragments arranged by size:
1917 1584 1384 814 613 268
AfeI AGC'GCT
Cuts at: 2359 2359
Size: 6580
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6383
AflIII A'CryG_T
Cuts at: 1203 2810 3318 5479 6456 1203
Size: 1607 508 2161 977 1327
Fragments arranged by size:
2161 1607 1327 977 508
AgeI A'CCGG_T
Cuts at: 3174 5411 3174
Size: 2237 4343
AhdI GACnn_n'nnGTC
Cuts at: 5309 5309
Size: 6580
AleI CACnn'nnGTG
Cuts at: 1166 3491 1166
Size: 2325 4255
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2613 1184
Size: 1429 5151
AlwNI CAG_nnn'CTG
Cuts at: 2587 3013 3401 4323 5895 2587
Size: 426 388 922 1572 3272
Fragments arranged by size:
3272 1572 922 426 388
ApaI G_GGCC'C
Cuts at: 2033 3289 3804 2033
Size: 1256 515 4809
Fragments arranged by size:
4809 1256 515
ApaLI G'TGCA_C
Cuts at: 2840 4637 5793 2840
Size: 1797 1156 3627
Fragments arranged by size:
3627 1797 1156
ApoI r'AATT_y
Cuts at: 1493 3889 4115 4267 6376 1493
Size: 2396 226 152 2109 1697
Fragments arranged by size:
2396 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5134 160
Size: 872 4102 1606
Fragments arranged by size:
4102 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
AvaI C'yCGr_G
Cuts at: 1870 1943 2174 2645 3414 3624 3905 1870
Size: 73 231 471 769 210 281 4545
Fragments arranged by size:
4545 769 471 281 231 210 73
BamHI G'GATC_C
Cuts at: 3910 4277 3910
Size: 367 6213
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2095 2396 3329 3335 3481
Size: 325 217 627 308 301 933 6 146
Cuts at: 3481 3901 5356 618
Size: 420 1455 1842
Fragments arranged by size:
1842 1455 933 627 420 325 308 301
217 146 6
BanII G_rGCy'C
Cuts at: 1391 2033 2181 3020 3289 3664 3804 3899 1391
Size: 642 148 839 269 375 140 95 4072
Fragments arranged by size:
4072 839 642 375 269 148 140 95
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3047 928
Size: 971 1148 4461
Fragments arranged by size:
4461 1148 971
BbvCI CC'TCA_GC
Cuts at: 2169 2169
Size: 6580
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4771 4805 1903
Size: 34 2834 34 3678
Fragments arranged by size:
3678 2834 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4472 5682 4472
Size: 1210 5370
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6580
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3144 5191 136
Size: 107 122 71 631 605 1472 2047 1525
Fragments arranged by size:
2047 1525 1472 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2217 6575 2217
Size: 4358 2222
BlpI GC'TnA_GC
Cuts at: 2547 3014 3997 2547
Size: 467 983 5130
Fragments arranged by size:
5130 983 467
Bme1580I G_kGCm'C
Cuts at: 1790 2033 2074 2100 2401 2541 2844 3289 3486
Size: 243 41 26 301 140 303 445 197
Cuts at: 3486 3804 4641 5797 1790
Size: 318 837 1156 2573
Fragments arranged by size:
2573 1156 837 445 318 303 301 243
197 140 41 26
BmgBI CAC'GTC
Cuts at: 1318 2515 2811 3319 1318
Size: 1197 296 508 4579
Fragments arranged by size:
4579 1197 508 296
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3292 3870 4311
Size: 416 53 83 399 24 1875 578 441
Cuts at: 4311 5269 442
Size: 958 1753
Fragments arranged by size:
1875 1753 958 578 441 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6580
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2249 2591 2705 3176 3798 5240 1239
Size: 902 108 342 114 471 622 1442 2579
Fragments arranged by size:
2579 1442 902 622 471 342 114 108
Bpu10I CC'TnA_GC
Cuts at: 2075 2169 2075
Size: 94 6486
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2576 4705 5570 5868 2576
Size: 2129 865 298 3288
Fragments arranged by size:
3288 2129 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5243 882
Size: 617 3744 2219
Fragments arranged by size:
3744 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4371 493
Size: 711 3167 2702
Fragments arranged by size:
3167 2702 711
BsaBI GATnn'nnATC
Cuts at: 3752 6574 3752
Size: 2822 3758
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2268 2748 3411 4387
Size: 53 83 186 936 735 480 663 976
Cuts at: 4387 4769 5404 275
Size: 382 635 1451
Fragments arranged by size:
1451 976 936 735 663 635 480 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2892 3174 3210 3576 5013 5411 5685 5832 6243
Size: 282 36 366 1437 398 274 147 411
Cuts at: 6243 2892
Size: 3229
Fragments arranged by size:
3229 1437 411 398 366 282 274 147
36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6355
Fragments arranged by size:
6355 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2138 2479 3038 3128 1186
Size: 952 341 559 90 4638
Fragments arranged by size:
4638 952 559 341 90
BseYI C'CCAG_C
Cuts at: 2144 2706 3167 3510 5783 6279 2144
Size: 562 461 343 2273 496 2445
Fragments arranged by size:
2445 2273 562 496 461 343
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6007
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3698 4030 4791 5819 1540
Size: 275 186 1697 332 761 1028 2301
Fragments arranged by size:
2301 1697 1028 761 332 275 186
BsiHKAI G_wGCw'C
Cuts at: 2618 2844 3264 3777 4641 4726 5797 2618
Size: 226 420 513 864 85 1071 3401
Fragments arranged by size:
3401 1071 864 513 420 226 85
BsmI GAATG_Cn'
Cuts at: 3553 3684 4091 4184 3553
Size: 131 407 93 5949
Fragments arranged by size:
5949 407 131 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2095 2918 3616 2095
Size: 823 698 5059
Fragments arranged by size:
5059 823 698
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2074 2100 2181 2401 2541 2618
Size: 399 243 41 26 81 220 140 77
Cuts at: 2618 2844 3020 3264 3289 3486 3664 3777 3804
Size: 226 176 244 25 197 178 113 27
Cuts at: 3804 3899 4641 4726 5797 1391
Size: 95 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 244 243 226 220
197 178 176 140 113 95 85 81
77 41 27 26 25
BspEI T'CCGG_A
Cuts at: 3210 3576 6243 3210
Size: 366 2667 3547
Fragments arranged by size:
3547 2667 366
BspHI T'CATG_A
Cuts at: 4469 6199 4469
Size: 1730 4850
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2572 2854 3104 3201 3935 844
Size: 1728 282 250 97 734 3489
Fragments arranged by size:
3489 1728 734 282 250 97
BsrBI CCG'CTC
Cuts at: 1394 1823 2560 4467 1394
Size: 429 737 1907 3507
Fragments arranged by size:
3507 1907 737 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2988 5075 5249 65
Size: 1183 1740 2087 174 1396
Fragments arranged by size:
2087 1740 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3174 3332 3697 5224 5411 1700
Size: 110 1364 158 365 1527 187 2869
Fragments arranged by size:
2869 1527 1364 365 187 158 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6580
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6580
BssSI C'ACGA_G
Cuts at: 2502 3185 3518 4640 5652 2502
Size: 683 333 1122 1012 3430
Fragments arranged by size:
3430 1122 1012 683 333
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2242 2587 794
Size: 1448 345 4787
Fragments arranged by size:
4787 1448 345
BstBI TT'CG_AA
Cuts at: 5384 5384
Size: 6580
BstEII G'GTnAC_C
Cuts at: 1184 2518 1184
Size: 1334 5246
BstXI CCAn_nnnn'nTGG
Cuts at: 2866 3346 2866
Size: 480 6100
BstYI r'GATC_y
Cuts at: 1972 2217 3213 3910 4277 4662 4679 6131 6217
Size: 245 996 697 367 385 17 1452 86
Cuts at: 6217 6229 6575 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 996 697 385 367 346 245
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3021 3593 3021
Size: 572 6008
BtgI C'CryG_G
Cuts at: 513 1065 2040 2454 2775 2982 3633 513
Size: 552 975 414 321 207 651 3460
Fragments arranged by size:
3460 975 651 552 414 321 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5841
Fragments arranged by size:
5841 683 56
BtsI GCAGTG_nn'
Cuts at: 2074 3457 3720 4096 4890 4910 2074
Size: 1383 263 376 794 20 3744
Fragments arranged by size:
3744 1383 794 376 263 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6580
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6545
DraI TTT'AAA
Cuts at: 2224 3884 2224
Size: 1660 4920
DraIII CAC_nnn'GTG
Cuts at: 2423 3520 3542 2423
Size: 1097 22 5461
Fragments arranged by size:
5461 1097 22
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5587 809
Size: 507 4271 1802
Fragments arranged by size:
4271 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2053 2457 2508
Size: 54 1425 50 275 18 223 404 51
Cuts at: 2508 2985 3522 3570 3695 4027 4916 6545 8
Size: 477 537 48 125 332 889 1629 43
Fragments arranged by size:
1629 1425 889 537 477 404 332 275
223 125 54 51 50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3695 4027 1537
Size: 275 1883 332 4090
Fragments arranged by size:
4090 1883 332 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4510 1353
Size: 3157 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5167 5541 5687 387
Size: 1064 138 3578 374 146 1280
Fragments arranged by size:
3578 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2245 2249 2591 2705 3059 3176
Size: 393 509 104 4 342 114 354 117
Cuts at: 3176 3798 4643 5240 6027 6295 1239
Size: 622 845 597 787 268 1524
Fragments arranged by size:
1524 845 787 622 597 509 393 354
342 268 117 114 104 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2123 2123
Size: 6580
EcoO109I rG'GnC_Cy
Cuts at: 2029 2165 2377 3145 3190 3475 3800 3801 3833
Size: 136 212 768 45 285 325 1 32
Cuts at: 3833 3864 2029
Size: 31 4745
Fragments arranged by size:
4745 768 325 285 212 136 45 32
31 1
EcoRI G'AATT_C
Cuts at: 3889 4267 3889
Size: 378 6202
FspI TGC'GCA
Cuts at: 2960 5086 2960
Size: 2126 4454
FspAI rTGC'GCAy
Cuts at: 2960 2960
Size: 6580
HaeII r_GCGC'y
Cuts at: 1678 2145 2278 2361 5727 1678
Size: 467 133 83 3366 2531
Fragments arranged by size:
3366 2531 467 133 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2704 2736 5221 5253 5295
Size: 32 135 32 1132 32 2485 32 42
Cuts at: 5295 5327 1373
Size: 32 2626
Fragments arranged by size:
2626 2485 1132 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3924 4170 669
Size: 988 281 1986 246 3079
Fragments arranged by size:
3079 1986 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6580
HpaI GTT'AAC
Cuts at: 4170 4170
Size: 6580
KpnI G_GTAC'C
Cuts at: 3905 3905
Size: 6580
MfeI C'AATT_G
Cuts at: 3290 4179 3290
Size: 889 5691
MluI A'CGCG_T
Cuts at: 6456 6456
Size: 6580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5694 5878 1305
Size: 4389 184 2007
Fragments arranged by size:
4389 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2055 2459 2987 3524 3572
Size: 54 1425 343 223 404 528 537 48
Cuts at: 3572 10
Size: 3018
Fragments arranged by size:
3018 1425 537 528 404 343 223 54
48
MslI CAynn'nnrTG
Cuts at: 518 1166 3491 3780 4538 4897 5056 518
Size: 648 2325 289 758 359 159 2042
Fragments arranged by size:
2325 2042 758 648 359 289 159
MspA1I CmG'CkG
Cuts at: 1606 2569 2683 2950 3996 4673 5821 6066 1606
Size: 963 114 267 1046 677 1148 245 2120
Fragments arranged by size:
2120 1148 1046 963 677 267 245 114
NcoI C'CATG_G
Cuts at: 513 1065 2040 2454 2982 3633 513
Size: 552 975 414 528 651 3460
Fragments arranged by size:
3460 975 651 552 528 414
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6580
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6580
NotI GC'GGCC_GC
Cuts at: 4027 4027
Size: 6580
NspI r_CATG'y
Cuts at: 1132 1229 3164 3938 5483 1132
Size: 97 1935 774 1545 2229
Fragments arranged by size:
2229 1935 1545 774 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6580
PciI A'CATG_T
Cuts at: 5479 5479
Size: 6580
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2983 1108
Size: 1875 4705
PfoI T'CCnGG_A
Cuts at: 2880 3276 3528 2880
Size: 396 252 5932
Fragments arranged by size:
5932 396 252
PmeI GTTT'AAAC
Cuts at: 3884 3884
Size: 6580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4649 4681 6162 6194 4649
Size: 32 1481 32 5035
Fragments arranged by size:
5035 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3190 3475 3833 3864 3190
Size: 285 358 31 5906
Fragments arranged by size:
5906 358 285 31
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6580
PsiI TTA'TAA
Cuts at: 4150 4150
Size: 6580
PspOMI G'GGCC_C
Cuts at: 2029 3285 3800 2029
Size: 1256 515 4809
Fragments arranged by size:
4809 1256 515
PspXI vC'TCGA_Gb
Cuts at: 2645 2645
Size: 6580
PstI C_TGCA'G
Cuts at: 830 2107 2238 2301 2365 2592 3659 3932 830
Size: 1277 131 63 64 227 1067 273 3478
Fragments arranged by size:
3478 1277 1067 273 227 131 64 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6580
PvuII CAG'CTG
Cuts at: 2569 2950 2569
Size: 381 6199
SalI G'TCGA_C
Cuts at: 1936 3922 1936
Size: 1986 4594
SbfI CC_TGCA'GG
Cuts at: 3932 3932
Size: 6580
ScaI AGT'ACT
Cuts at: 4828 6540 4828
Size: 1712 4868
SexAI A'CCwGG_T
Cuts at: 3477 3477
Size: 6580
SfcI C'TryA_G
Cuts at: 826 1045 2103 2154 2182 2234 2297 2361 2588
Size: 219 1058 51 28 52 63 64 227
Cuts at: 2588 3655 3928 5063 5744 5935 826
Size: 1067 273 1135 681 191 1471
Fragments arranged by size:
1471 1135 1067 1058 681 273 227 219
191 64 63 52 51 28
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6580
SmaI CCC'GGG
Cuts at: 1872 2176 3416 3626 3907 1872
Size: 304 1240 210 281 4545
Fragments arranged by size:
4545 1240 304 281 210
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2555 2645 4684 5585 5847 820
Size: 197 926 612 90 2039 901 262 1553
Fragments arranged by size:
2039 1553 926 901 612 262 197 90
SnaBI TAC'GTA
Cuts at: 493 4371 493
Size: 3878 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6580
SphI G_CATG'C
Cuts at: 1132 3938 1132
Size: 2806 3774
SrfI GCCC'GGGC
Cuts at: 2176 2176
Size: 6580
SspI AAT'ATT
Cuts at: 5 52 4504 5
Size: 47 4452 2081
Fragments arranged by size:
4452 2081 47
StuI AGG'CCT
Cuts at: 1517 2672 2876 1517
Size: 1155 204 5221
Fragments arranged by size:
5221 1155 204
StyI C'CwwG_G
Cuts at: 513 1065 2040 2442 2454 2601 2982 3558 3633
Size: 552 975 402 12 147 381 576 75
Cuts at: 3633 4019 513
Size: 386 3074
Fragments arranged by size:
3074 975 576 552 402 386 381 147
75 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4607 4624 4777 4962 5407 325
Size: 1255 3027 17 153 185 445 1498
Fragments arranged by size:
3027 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2796 4826 6538
Size: 274 80 33 51 1443 819 2030 1712
Cuts at: 6538 96
Size: 138
Fragments arranged by size:
2030 1712 1443 819 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6540 6572 6540
Size: 32 6548
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6580
XbaI T'CTAG_A
Cuts at: 3916 3916
Size: 6580
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2863 3565 2863
Size: 702 5878
XhoI C'TCGA_G
Cuts at: 1943 2645 1943
Size: 702 5878
XmaI C'CCGG_G
Cuts at: 1870 2174 3414 3624 3905 1870
Size: 304 1240 210 281 4545
Fragments arranged by size:
4545 1240 304 281 210
XmnI GAAnn'nnTTC
Cuts at: 2876 4709 2876
Size: 1833 4747
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2269 4388 276
Size: 53 83 186 936 735 2119 2468
Fragments arranged by size:
2468 2119 936 735 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I EcoICRI EcoRV FalI FseI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SwaI