Restriction map for
pFN21AE0335
(Circular) (Six-base) MAPSORT of: pFN21AE0335.seq Check: 5345 from: 1 to: 7447
pFN21AE0335 7447 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5257 278
Size: 53 83 186 936 3721 2468
Fragments arranged by size:
3721 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2427 4790 1937
Size: 490 2363 4594
Fragments arranged by size:
4594 2363 490
Acc65I G'GTAC_C
Cuts at: 3900 4768 3900
Size: 868 6579
AclI AA'CG_TT
Cuts at: 1563 5574 5947 1563
Size: 4011 373 3063
Fragments arranged by size:
4011 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2414 2460 4413 4585 5510 6894 7162 1632
Size: 782 46 1953 172 925 1384 268 1917
Fragments arranged by size:
1953 1917 1384 925 782 268 172 46
AfeI AGC'GCT
Cuts at: 4022 4022
Size: 7447
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7250
AflIII A'CryG_T
Cuts at: 1203 3713 4066 6346 7323 1203
Size: 2510 353 2280 977 1327
Fragments arranged by size:
2510 2280 1327 977 353
AgeI A'CCGG_T
Cuts at: 6278 6278
Size: 7447
AhdI GACnn_n'nnGTC
Cuts at: 2434 6176 2434
Size: 3742 3705
AleI CACnn'nnGTG
Cuts at: 1166 2966 1166
Size: 1800 5647
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2984 3603 1184
Size: 1800 619 5028
Fragments arranged by size:
5028 1800 619
AlwNI CAG_nnn'CTG
Cuts at: 2073 2897 2936 5190 6762 2073
Size: 824 39 2254 1572 2758
Fragments arranged by size:
2758 2254 1572 824 39
ApaI G_GGCC'C
Cuts at: 2930 2930
Size: 7447
ApaLI G'TGCA_C
Cuts at: 2969 5504 6660 2969
Size: 2535 1156 3756
Fragments arranged by size:
3756 2535 1156
ApoI r'AATT_y
Cuts at: 1493 2223 2464 2597 2606 3792 4756 4982 5134
Size: 730 241 133 9 1186 964 226 152
Cuts at: 5134 7243 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1186 964 730 241 226 152
133 9
AseI AT'TA_AT
Cuts at: 160 1032 6001 160
Size: 872 4969 1606
Fragments arranged by size:
4969 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7447
AvaI C'yCGr_G
Cuts at: 1870 1943 4772 1870
Size: 73 2829 4545
Fragments arranged by size:
4545 2829 73
BamHI G'GATC_C
Cuts at: 2685 4777 5144 2685
Size: 2092 367 4988
Fragments arranged by size:
4988 2092 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3746 3900 4768 6223 618
Size: 325 217 627 1959 154 868 1455 1842
Fragments arranged by size:
1959 1842 1455 868 627 325 217 154
BanII G_rGCy'C
Cuts at: 1391 2930 3624 3969 4766 1391
Size: 1539 694 345 797 4072
Fragments arranged by size:
4072 1539 797 694 345
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2172 3028 3405 928
Size: 971 273 856 377 4970
Fragments arranged by size:
4970 971 856 377 273
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5638 5672 1903
Size: 34 3701 34 3678
Fragments arranged by size:
3701 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2762 3089 5339 6549 2762
Size: 327 2250 1210 3660
Fragments arranged by size:
3660 2250 1210 327
BclI T'GATC_A
Cuts at: 1548 4527 1548
Size: 2979 4468
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6058 136
Size: 107 122 71 631 605 4386 1525
Fragments arranged by size:
4386 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2575 2954 4584 7442 2575
Size: 379 1630 2858 2580
Fragments arranged by size:
2858 2580 1630 379
BlpI GC'TnA_GC
Cuts at: 4864 4864
Size: 7447
Bme1580I G_kGCm'C
Cuts at: 1790 2930 2973 5508 6664 1790
Size: 1140 43 2535 1156 2573
Fragments arranged by size:
2573 2535 1156 1140 43
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7447
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2840 2924 3837
Size: 416 53 83 399 24 1423 84 913
Cuts at: 3837 4286 4738 5178 6136 442
Size: 449 452 440 958 1753
Fragments arranged by size:
1753 1423 958 913 452 449 440 416
399 84 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7447
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4723 4723
Size: 7447
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3930 4718 6107 1239
Size: 2691 788 1389 2579
Fragments arranged by size:
2691 2579 1389 788
Bpu10I CC'TnA_GC
Cuts at: 3278 4691 3278
Size: 1413 6034
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5572 6437 6735 5572
Size: 865 298 6284
Fragments arranged by size:
6284 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2382 3431 3691 6110 882
Size: 617 883 1049 260 2419 2219
Fragments arranged by size:
2419 2219 1049 883 617 260
BsaAI yAC'GTr
Cuts at: 493 1204 4069 4668 5238 493
Size: 711 2865 599 570 2702
Fragments arranged by size:
2865 2702 711 599 570
BsaBI GATnn'nnATC
Cuts at: 7441 7441
Size: 7447
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2098 5254 5636 6271
Size: 53 83 186 936 565 3156 382 635
Cuts at: 6271 275
Size: 1451
Fragments arranged by size:
3156 1451 936 635 565 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2299 5880 6278 6552 6699 7110 2299
Size: 3581 398 274 147 411 2636
Fragments arranged by size:
3581 2636 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2799 2829 1178
Size: 30 165 30 1396 30 5796
Fragments arranged by size:
5796 1396 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2265 2549 2552 3421 1186
Size: 1079 284 3 869 5212
Fragments arranged by size:
5212 1079 869 284 3
BseYI C'CCAG_C
Cuts at: 4153 4562 6650 7146 4153
Size: 409 2088 496 4454
Fragments arranged by size:
4454 2088 496 409
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6874
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4897 5658 6686 1540
Size: 275 186 2896 761 1028 2301
Fragments arranged by size:
2896 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2973 3207 3615 3624 5508 5593 6664 2973
Size: 234 408 9 1884 85 1071 3756
Fragments arranged by size:
3756 1884 1071 408 234 85 9
BsmI GAATG_Cn'
Cuts at: 2866 3592 4958 5051 2866
Size: 726 1366 93 5262
Fragments arranged by size:
5262 1366 726 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2930 2973 3207 3615 3624 3969 4766
Size: 399 1140 43 234 408 9 345 797
Cuts at: 4766 5508 5593 6664 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1140 1071 797 742 408 399 345
234 85 43 9
BspEI T'CCGG_A
Cuts at: 2299 7110 2299
Size: 4811 2636
BspHI T'CATG_A
Cuts at: 4086 5336 7066 4086
Size: 1250 1730 4467
Fragments arranged by size:
4467 1730 1250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4802 844
Size: 3958 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5334 1394
Size: 429 3511 3507
Fragments arranged by size:
3511 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4716 5942 6116 65
Size: 1183 3468 1226 174 1396
Fragments arranged by size:
3468 1396 1226 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6091 6278 1700
Size: 110 4281 187 2869
Fragments arranged by size:
4281 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7447
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7447
BssSI C'ACGA_G
Cuts at: 5507 6519 5507
Size: 1012 6435
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3149 3274 3577 4492 794
Size: 2355 125 303 915 3749
Fragments arranged by size:
3749 2355 915 303 125
BstBI TT'CG_AA
Cuts at: 6251 6251
Size: 7447
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7447
BstXI CCAn_nnnn'nTGG
Cuts at: 3193 3193
Size: 7447
BstYI r'GATC_y
Cuts at: 1972 2575 2685 2954 3506 3831 4287 4584 4777
Size: 603 110 269 552 325 456 297 193
Cuts at: 4777 5144 5529 5546 6998 7084 7096 7442 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 603 552 456 385 367 346
325 297 269 193 110 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3931 513
Size: 552 939 1927 4029
Fragments arranged by size:
4029 1927 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6708
Fragments arranged by size:
6708 683 56
BtsI GCAGTG_nn'
Cuts at: 2022 3150 4963 5757 5777 2022
Size: 1128 1813 794 20 3692
Fragments arranged by size:
3692 1813 1128 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7447
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4186 4221 529
Size: 35 3622 35 3755
Fragments arranged by size:
3755 3622 35 35
DraI TTT'AAA
Cuts at: 4751 4751
Size: 7447
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4733 6454 809
Size: 507 3417 1721 1802
Fragments arranged by size:
3417 1802 1721 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3419 3934 4456
Size: 54 1425 50 275 18 1589 515 522
Cuts at: 4456 4894 5783 7412 8
Size: 438 889 1629 43
Fragments arranged by size:
1629 1589 1425 889 522 515 438 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4894 1537
Size: 275 3082 4090
Fragments arranged by size:
4090 3082 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2262 2439 2586 3527 5377 1353
Size: 909 177 147 941 1850 3423
Fragments arranged by size:
3423 1850 941 909 177 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6034 6408 6554 387
Size: 1064 138 4445 374 146 1280
Fragments arranged by size:
4445 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3622 3622
Size: 7447
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2414 2460 3930 4413 4585 4718 5510
Size: 393 782 46 1470 483 172 133 792
Cuts at: 5510 6107 6894 7162 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1470 792 787 782 597 483 393
268 172 133 46
EcoO109I rG'GnC_Cy
Cuts at: 3017 3017
Size: 7447
EcoRI G'AATT_C
Cuts at: 4756 5134 4756
Size: 378 7069
EcoRV GAT'ATC
Cuts at: 2617 2617
Size: 7447
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2446 3241 2446
Size: 795 6652
FspI TGC'GCA
Cuts at: 5953 5953
Size: 7447
HaeII r_GCGC'y
Cuts at: 1678 4024 6594 1678
Size: 2346 2570 2531
Fragments arranged by size:
2570 2531 2346
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2420 2452 2528 2560 3996
Size: 32 135 32 848 32 76 32 1436
Cuts at: 3996 4028 4243 4275 6088 6120 6162 6194 1373
Size: 32 215 32 1813 32 42 32 2626
Fragments arranged by size:
2626 1813 1436 848 215 135 76 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4791 5037 669
Size: 988 281 2853 246 3079
Fragments arranged by size:
3079 2853 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3296 748
Size: 2548 4899
HpaI GTT'AAC
Cuts at: 5037 5037
Size: 7447
KpnI G_GTAC'C
Cuts at: 3904 4772 3904
Size: 868 6579
MfeI C'AATT_G
Cuts at: 3604 5046 3604
Size: 1442 6005
MluI A'CGCG_T
Cuts at: 7323 7323
Size: 7447
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6561 6745 1305
Size: 5256 184 2007
Fragments arranged by size:
5256 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3421 3936 4458 10
Size: 54 1425 343 1589 515 522 2999
Fragments arranged by size:
2999 1589 1425 522 515 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2679 2966 5405 5764 5923 518
Size: 648 1513 287 2439 359 159 2042
Fragments arranged by size:
2439 2042 1513 648 359 287 159
MspA1I CmG'CkG
Cuts at: 1606 3111 4863 5540 6688 6933 1606
Size: 1505 1752 677 1148 245 2120
Fragments arranged by size:
2120 1752 1505 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5956
Fragments arranged by size:
5956 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7447
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7447
NotI GC'GGCC_GC
Cuts at: 4894 4894
Size: 7447
NspI r_CATG'y
Cuts at: 1132 1229 2699 4153 4805 6350 1132
Size: 97 1470 1454 652 1545 2229
Fragments arranged by size:
2229 1545 1470 1454 652 97
PasI CC'CwG_GG
Cuts at: 1348 2470 1348
Size: 1122 6325
PciI A'CATG_T
Cuts at: 6346 6346
Size: 7447
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2830 4374 4728 1108
Size: 1722 1544 354 3827
Fragments arranged by size:
3827 1722 1544 354
PfoI T'CCnGG_A
Cuts at: 4219 4219
Size: 7447
PmeI GTTT'AAAC
Cuts at: 4751 4751
Size: 7447
PmlI CAC'GTG
Cuts at: 4069 4069
Size: 7447
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5516 5548 7029 7061 5516
Size: 32 1481 32 5902
Fragments arranged by size:
5902 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3017 3017
Size: 7447
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7447
PsiI TTA'TAA
Cuts at: 2751 5017 2751
Size: 2266 5181
PspOMI G'GGCC_C
Cuts at: 2926 2926
Size: 7447
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4037 4069 4037
Size: 32 7415
PstI C_TGCA'G
Cuts at: 830 2902 3664 4799 830
Size: 2072 762 1135 3478
Fragments arranged by size:
3478 2072 1135 762
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7447
SacI G_AGCT'C
Cuts at: 3624 3624
Size: 7447
SalI G'TCGA_C
Cuts at: 1936 4789 1936
Size: 2853 4594
SbfI CC_TGCA'GG
Cuts at: 4799 4799
Size: 7447
ScaI AGT'ACT
Cuts at: 3047 5695 7407 3047
Size: 2648 1712 3087
Fragments arranged by size:
3087 2648 1712
SexAI A'CCwGG_T
Cuts at: 2174 2174
Size: 7447
SfcI C'TryA_G
Cuts at: 826 1045 2049 2495 2841 2898 3660 4356 4795
Size: 219 1004 446 346 57 762 696 439
Cuts at: 4795 5930 6611 6802 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1004 762 696 681 446 439
346 219 191 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7447
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7447
SmaI CCC'GGG
Cuts at: 1872 4774 1872
Size: 2902 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5551 6452 6714 820
Size: 197 926 3608 901 262 1553
Fragments arranged by size:
3608 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4668 5238 493
Size: 4175 570 2702
Fragments arranged by size:
4175 2702 570
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7447
SphI G_CATG'C
Cuts at: 1132 4805 1132
Size: 3673 3774
SspI AAT'ATT
Cuts at: 5 52 5371 5
Size: 47 5319 2081
Fragments arranged by size:
5319 2081 47
StuI AGG'CCT
Cuts at: 1517 4093 1517
Size: 2576 4871
StyI C'CwwG_G
Cuts at: 513 1065 2004 3456 4299 4886 513
Size: 552 939 1452 843 587 3074
Fragments arranged by size:
3074 1452 939 843 587 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4424 5474 5491 5644 5829 6274 325
Size: 1255 2844 1050 17 153 185 445 1498
Fragments arranged by size:
2844 1498 1255 1050 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2907 3045 4277
Size: 274 80 33 51 1443 930 138 1232
Cuts at: 4277 5693 7405 96
Size: 1416 1712 138
Fragments arranged by size:
1712 1443 1416 1232 930 274 138 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2595 2627 4058 4090 4259 4291 7407 7439 2595
Size: 32 1431 32 169 32 3116 32 2603
Fragments arranged by size:
3116 2603 1431 169 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7447
XbaI T'CTAG_A
Cuts at: 4783 4783
Size: 7447
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4570 4637 4693 4570
Size: 67 56 7324
Fragments arranged by size:
7324 67 56
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7447
XmaI C'CCGG_G
Cuts at: 1870 4772 1870
Size: 2902 4545
XmnI GAAnn'nnTTC
Cuts at: 5576 5576
Size: 7447
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5255 276
Size: 53 83 186 936 3721 2468
Fragments arranged by size:
3721 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI
PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI