Restriction map for
pFN21AE0334
(Circular) (Six-base) MAPSORT of: pFN21AE0334.seq Check: 6333 from: 1 to: 7330
pFN21AE0334 7330 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5140 278
Size: 53 83 186 936 3604 2468
Fragments arranged by size:
3604 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4673 1937
Size: 2736 4594
Acc65I G'GTAC_C
Cuts at: 4651 4651
Size: 7330
AclI AA'CG_TT
Cuts at: 1563 4629 5457 5830 1563
Size: 3066 828 373 3063
Fragments arranged by size:
3066 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2649 3579 3670 5393 6777 7045 1632
Size: 1017 930 91 1723 1384 268 1917
Fragments arranged by size:
1917 1723 1384 1017 930 268 91
AfeI AGC'GCT
Cuts at: 2709 2709
Size: 7330
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7133
AflIII A'CryG_T
Cuts at: 1203 2698 3231 4506 6229 7206 1203
Size: 1495 533 1275 1723 977 1327
Fragments arranged by size:
1723 1495 1327 1275 977 533
AgeI A'CCGG_T
Cuts at: 6161 6161
Size: 7330
AhdI GACnn_n'nnGTC
Cuts at: 6059 6059
Size: 7330
AleI CACnn'nnGTG
Cuts at: 1166 3978 1166
Size: 2812 4518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4587 1184
Size: 3403 3927
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2738 2770 2738
Size: 32 7298
AlwNI CAG_nnn'CTG
Cuts at: 3001 3478 4072 5073 6645 3001
Size: 477 594 1001 1572 3686
Fragments arranged by size:
3686 1572 1001 594 477
ApaLI G'TGCA_C
Cuts at: 5387 6543 5387
Size: 1156 6174
ApoI r'AATT_y
Cuts at: 1493 2318 2517 3903 4205 4358 4639 4865 5017
Size: 825 199 1386 302 153 281 226 152
Cuts at: 5017 7126 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1386 825 302 281 226 199
153 152
AseI AT'TA_AT
Cuts at: 160 1032 4599 5884 160
Size: 872 3567 1285 1606
Fragments arranged by size:
3567 1606 1285 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7330
AvaI C'yCGr_G
Cuts at: 1870 1943 4655 1870
Size: 73 2712 4545
Fragments arranged by size:
4545 2712 73
BamHI G'GATC_C
Cuts at: 2746 3510 4660 5027 2746
Size: 764 1150 367 5049
Fragments arranged by size:
5049 1150 764 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3646 4651 6106 618
Size: 325 217 627 1859 1005 1455 1842
Fragments arranged by size:
1859 1842 1455 1005 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2721 4649 1391
Size: 1330 1928 4072
Fragments arranged by size:
4072 1928 1330
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2586 928
Size: 971 687 5672
Fragments arranged by size:
5672 971 687
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2246 2280 5521 5555 1903
Size: 34 309 34 3241 34 3678
Fragments arranged by size:
3678 3241 309 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2675 5222 6432 2675
Size: 2547 1210 3573
Fragments arranged by size:
3573 2547 1210
BclI T'GATC_A
Cuts at: 1548 2428 4366 1548
Size: 880 1938 4512
Fragments arranged by size:
4512 1938 880
BfrBI ATG'CAT
Cuts at: 2171 2738 2171
Size: 567 6763
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5941 136
Size: 107 122 71 631 605 4269 1525
Fragments arranged by size:
4269 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7325 7325
Size: 7330
BlpI GC'TnA_GC
Cuts at: 2955 4747 2955
Size: 1792 5538
Bme1580I G_kGCm'C
Cuts at: 1790 5391 6547 1790
Size: 3601 1156 2573
Fragments arranged by size:
3601 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7330
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2125 5061 6019
Size: 416 53 83 399 24 708 2936 958
Cuts at: 6019 442
Size: 1753
Fragments arranged by size:
2936 1753 958 708 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2999 3947 5990 1239
Size: 1760 948 2043 2579
Fragments arranged by size:
2579 2043 1760 948
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2437 5455 6320 6618 2437
Size: 3018 865 298 3149
Fragments arranged by size:
3149 3018 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5993 882
Size: 617 4494 2219
Fragments arranged by size:
4494 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4507 5121 493
Size: 711 3303 614 2702
Fragments arranged by size:
3303 2702 711 614
BsaBI GATnn'nnATC
Cuts at: 3969 4251 7324 3969
Size: 282 3073 3975
Fragments arranged by size:
3975 3073 282
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5137 5519 6154 275
Size: 53 83 186 936 3604 382 635 1451
Fragments arranged by size:
3604 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3768 5763 6161 6435 6582 6993 3768
Size: 1995 398 274 147 411 4105
Fragments arranged by size:
4105 1995 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3566 3596 1178
Size: 30 165 30 2163 30 4912
Fragments arranged by size:
4912 2163 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3443 4215 1186
Size: 2257 772 4301
Fragments arranged by size:
4301 2257 772
BseYI C'CCAG_C
Cuts at: 2021 2055 4023 6533 7029 2021
Size: 34 1968 2510 496 2322
Fragments arranged by size:
2510 2322 1968 496 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2289 3116 3779 3936 1163
Size: 573 553 827 663 157 4557
Fragments arranged by size:
4557 827 663 573 553 157
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2094 4780 5541 6569 1540
Size: 275 186 93 2686 761 1028 2301
Fragments arranged by size:
2686 2301 1028 761 275 186 93
BsiHKAI G_wGCw'C
Cuts at: 2453 5391 5476 6547 2453
Size: 2938 85 1071 3236
Fragments arranged by size:
3236 2938 1071 85
BsmI GAATG_Cn'
Cuts at: 2799 4841 4934 2799
Size: 2042 93 5195
Fragments arranged by size:
5195 2042 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2453 2721 4649 5391 5476 6547 1391
Size: 399 663 268 1928 742 85 1071 2174
Fragments arranged by size:
2174 1928 1071 742 663 399 268 85
BspEI T'CCGG_A
Cuts at: 3768 6993 3768
Size: 3225 4105
BspHI T'CATG_A
Cuts at: 2431 5219 6949 2431
Size: 2788 1730 2812
Fragments arranged by size:
2812 2788 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4685 844
Size: 3841 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 3887 5217 1394
Size: 429 188 1876 1330 3507
Fragments arranged by size:
3507 1876 1330 429 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 3938 4281 5825 5999 65
Size: 1183 2690 343 1544 174 1396
Fragments arranged by size:
2690 1544 1396 1183 343 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2015 2325 5974 6161 1700
Size: 110 205 310 3649 187 2869
Fragments arranged by size:
3649 2869 310 205 187 110
BsrGI T'GTAC_A
Cuts at: 96 3362 3725 4185 96
Size: 3266 363 460 3241
Fragments arranged by size:
3266 3241 460 363
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7330
BssSI C'ACGA_G
Cuts at: 2265 5390 6402 2265
Size: 3125 1012 3193
Fragments arranged by size:
3193 3125 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2177 794
Size: 1383 5947
BstBI TT'CG_AA
Cuts at: 6134 6134
Size: 7330
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7330
BstXI CCAn_nnnn'nTGG
Cuts at: 2161 3562 2161
Size: 1401 5929
BstYI r'GATC_y
Cuts at: 1972 2746 3510 4246 4660 5027 5412 5429 6881
Size: 774 764 736 414 367 385 17 1452
Cuts at: 6881 6967 6979 7325 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 774 764 736 414 385 367
346 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2533 3549 3976 513
Size: 552 939 529 1016 427 3867
Fragments arranged by size:
3867 1016 939 552 529 427
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6591
Fragments arranged by size:
6591 683 56
BtsI GCAGTG_nn'
Cuts at: 2972 3347 3543 4846 5640 5660 2972
Size: 375 196 1303 794 20 4642
Fragments arranged by size:
4642 1303 794 375 196 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7330
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7295
DraI TTT'AAA
Cuts at: 4634 4634
Size: 7330
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4507 6337 809
Size: 507 3191 1830 1802
Fragments arranged by size:
3191 1830 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3552 4612 4777
Size: 54 1425 50 275 18 1722 1060 165
Cuts at: 4777 5666 7295 8
Size: 889 1629 43
Fragments arranged by size:
1722 1629 1425 1060 889 275 165 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4777 1537
Size: 275 2965 4090
Fragments arranged by size:
4090 2965 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2826 5260 1353
Size: 1473 2434 3423
Fragments arranged by size:
3423 2434 1473
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5917 6291 6437 387
Size: 1064 138 4328 374 146 1280
Fragments arranged by size:
4328 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2649 2999 3579 3670 3947 5393 5990
Size: 393 1017 350 580 91 277 1446 597
Cuts at: 5990 6777 7045 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1446 1017 787 597 580 393 350
277 268 91
EcoO109I rG'GnC_Cy
Cuts at: 4495 4567 4495
Size: 72 7258
EcoRI G'AATT_C
Cuts at: 4639 5017 4639
Size: 378 6952
EcoRV GAT'ATC
Cuts at: 2544 2544
Size: 7330
FseI GG_CCGG'CC
Cuts at: 2019 2019
Size: 7330
FspI TGC'GCA
Cuts at: 5836 5836
Size: 7330
HaeII r_GCGC'y
Cuts at: 1678 2711 6477 1678
Size: 1033 3766 2531
Fragments arranged by size:
3766 2531 1033
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2072 2104 2148 2180 2435
Size: 32 135 32 500 32 44 32 255
Cuts at: 2435 2467 3566 3598 3611 3643 5971 6003 6045
Size: 32 1099 32 13 32 2328 32 42
Cuts at: 6045 6077 1373
Size: 32 2626
Fragments arranged by size:
2626 2328 1099 500 255 135 44 42
32 32 32 32 32 32 32 32
32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2770 4674 4920 669
Size: 988 281 832 1904 246 3079
Fragments arranged by size:
3079 1904 988 832 281 246
HindIII A'AGCT_T
Cuts at: 748 3483 748
Size: 2735 4595
HpaI GTT'AAC
Cuts at: 4920 4920
Size: 7330
KpnI G_GTAC'C
Cuts at: 4655 4655
Size: 7330
MfeI C'AATT_G
Cuts at: 4929 4929
Size: 7330
MluI A'CGCG_T
Cuts at: 7206 7206
Size: 7330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6444 6628 1305
Size: 5139 184 2007
Fragments arranged by size:
5139 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3554 10
Size: 54 1425 343 1722 3786
Fragments arranged by size:
3786 1722 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 2493 2532 3092 3524 3978 5288
Size: 648 1035 292 39 560 432 454 1310
Cuts at: 5288 5647 5806 518
Size: 359 159 2042
Fragments arranged by size:
2042 1310 1035 648 560 454 432 359
292 159 39
MspA1I CmG'CkG
Cuts at: 1606 4746 5423 6571 6816 1606
Size: 3140 677 1148 245 2120
Fragments arranged by size:
3140 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 2017 2017
Size: 7330
NcoI C'CATG_G
Cuts at: 513 1065 2004 2533 3549 513
Size: 552 939 529 1016 4294
Fragments arranged by size:
4294 1016 939 552 529
NdeI CA'TA_TG
Cuts at: 387 2740 387
Size: 2353 4977
NgoMIV G'CCGG_C
Cuts at: 2015 2015
Size: 7330
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7330
NotI GC'GGCC_GC
Cuts at: 4777 4777
Size: 7330
NsiI A_TGCA'T
Cuts at: 2173 2740 2173
Size: 567 6763
NspI r_CATG'y
Cuts at: 1132 1229 2702 3235 3857 3880 4688 6233 1132
Size: 97 1473 533 622 23 808 1545 2229
Fragments arranged by size:
2229 1545 1473 808 622 533 97 23
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7330
PciI A'CATG_T
Cuts at: 2698 3231 6229 2698
Size: 533 2998 3799
Fragments arranged by size:
3799 2998 533
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3967 1108
Size: 2859 4471
PmeI GTTT'AAAC
Cuts at: 4634 4634
Size: 7330
PmlI CAC'GTG
Cuts at: 4507 4507
Size: 7330
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5399 5431 6912 6944 5399
Size: 32 1481 32 5785
Fragments arranged by size:
5785 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7330
PsiI TTA'TAA
Cuts at: 4220 4900 4220
Size: 680 6650
PstI C_TGCA'G
Cuts at: 830 4544 4682 830
Size: 3714 138 3478
Fragments arranged by size:
3714 3478 138
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7330
SalI G'TCGA_C
Cuts at: 1936 4672 1936
Size: 2736 4594
SbfI CC_TGCA'GG
Cuts at: 4682 4682
Size: 7330
ScaI AGT'ACT
Cuts at: 5578 7290 5578
Size: 1712 5618
SfcI C'TryA_G
Cuts at: 826 1045 3623 4170 4540 4678 5813 6494 6685
Size: 219 2578 547 370 138 1135 681 191
Cuts at: 6685 826
Size: 1471
Fragments arranged by size:
2578 1471 1135 681 547 370 219 191
138
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7330
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7330
SmaI CCC'GGG
Cuts at: 1872 4657 1872
Size: 2785 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2452 5434 6335 6597 820
Size: 197 926 509 2982 901 262 1553
Fragments arranged by size:
2982 1553 926 901 509 262 197
SnaBI TAC'GTA
Cuts at: 493 5121 493
Size: 4628 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7330
SphI G_CATG'C
Cuts at: 1132 4688 1132
Size: 3556 3774
SspI AAT'ATT
Cuts at: 5 52 5254 5
Size: 47 5202 2081
Fragments arranged by size:
5202 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7330
StyI C'CwwG_G
Cuts at: 513 1065 2004 2420 2533 3549 4769 513
Size: 552 939 416 113 1016 1220 3074
Fragments arranged by size:
3074 1220 1016 939 552 416 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2080 5357 5374 5527 5712 6157 325
Size: 1255 500 3277 17 153 185 445 1498
Fragments arranged by size:
3277 1498 1255 500 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3362 3725 4185
Size: 274 80 33 51 1443 1385 363 460
Cuts at: 4185 5576 7288 96
Size: 1391 1712 138
Fragments arranged by size:
1712 1443 1391 1385 460 363 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3258 3290 3790 3822 7290 7322 3258
Size: 32 500 32 3468 32 3266
Fragments arranged by size:
3468 3266 500 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2090 1316
Size: 774 6556
XbaI T'CTAG_A
Cuts at: 4666 4666
Size: 7330
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2158 3115 2158
Size: 957 6373
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7330
XmaI C'CCGG_G
Cuts at: 1870 4655 1870
Size: 2785 4545
XmnI GAAnn'nnTTC
Cuts at: 3474 5459 3474
Size: 1985 5345
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5138 276
Size: 53 83 186 936 3604 2468
Fragments arranged by size:
3604 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FalI FspAI KasI NarI NruI PacI PfoI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI