Restriction map for
pFN21AE0333
(Circular) (Six-base) MAPSORT of: pFN21AE0333.seq Check: 5901 from: 1 to: 6604
pFN21AE0333 6604 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4414 278
Size: 53 83 186 936 2878 2468
Fragments arranged by size:
2878 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2385 3947 1937
Size: 448 1562 4594
Fragments arranged by size:
4594 1562 448
Acc65I G'GTAC_C
Cuts at: 3925 3925
Size: 6604
AclI AA'CG_TT
Cuts at: 1563 4731 5104 1563
Size: 3168 373 3063
Fragments arranged by size:
3168 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2585 3614 4667 6051 6319 1632
Size: 953 1029 1053 1384 268 1917
Fragments arranged by size:
1917 1384 1053 1029 953 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6407
AflIII A'CryG_T
Cuts at: 1203 5503 6480 1203
Size: 4300 977 1327
Fragments arranged by size:
4300 1327 977
AgeI A'CCGG_T
Cuts at: 5435 5435
Size: 6604
AhdI GACnn_n'nnGTC
Cuts at: 5333 5333
Size: 6604
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6604
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6604
AlwNI CAG_nnn'CTG
Cuts at: 2419 2867 3058 4347 5919 2419
Size: 448 191 1289 1572 3104
Fragments arranged by size:
3104 1572 1289 448 191
ApaLI G'TGCA_C
Cuts at: 4661 5817 4661
Size: 1156 5448
ApoI r'AATT_y
Cuts at: 1493 2783 3137 3913 4139 4291 6400 1493
Size: 1290 354 776 226 152 2109 1697
Fragments arranged by size:
2109 1697 1290 776 354 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3845 5158 160
Size: 872 2813 1313 1606
Fragments arranged by size:
2813 1606 1313 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6604
AvaI C'yCGr_G
Cuts at: 1870 1943 3929 1870
Size: 73 1986 4545
Fragments arranged by size:
4545 1986 73
BamHI G'GATC_C
Cuts at: 2776 3934 4301 2776
Size: 1158 367 5079
Fragments arranged by size:
5079 1158 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3925 5380 618
Size: 325 217 627 2138 1455 1842
Fragments arranged by size:
2138 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2210 2448 3243 3875 3923 1391
Size: 819 238 795 632 48 4072
Fragments arranged by size:
4072 819 795 632 238 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3373 928
Size: 971 1474 4159
Fragments arranged by size:
4159 1474 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4795 4829 1903
Size: 34 2858 34 3678
Fragments arranged by size:
3678 2858 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4496 5706 4496
Size: 1210 5394
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6604
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5215 136
Size: 107 122 71 631 605 3543 1525
Fragments arranged by size:
3543 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6599 6599
Size: 6604
BlpI GC'TnA_GC
Cuts at: 4021 4021
Size: 6604
Bme1580I G_kGCm'C
Cuts at: 1790 2256 3300 4665 5821 1790
Size: 466 1044 1365 1156 2573
Fragments arranged by size:
2573 1365 1156 1044 466
BmgBI CAC'GTC
Cuts at: 1318 3067 1318
Size: 1749 4855
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2877 4335 5293
Size: 416 53 83 399 24 1460 1458 958
Cuts at: 5293 442
Size: 1753
Fragments arranged by size:
1753 1460 1458 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2441 2579 2624 2957 3035 3312 3416 5264
Size: 1202 138 45 333 78 277 104 1848
Cuts at: 5264 1239
Size: 2579
Fragments arranged by size:
2579 1848 1202 333 277 138 104 78
45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3543 4729 5594 5892 3543
Size: 1186 865 298 4255
Fragments arranged by size:
4255 1186 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5267 882
Size: 617 3768 2219
Fragments arranged by size:
3768 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4395 493
Size: 711 3191 2702
Fragments arranged by size:
3191 2702 711
BsaBI GATnn'nnATC
Cuts at: 3129 6598 3129
Size: 3469 3135
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4411 4793 5428 275
Size: 53 83 186 936 2878 382 635 1451
Fragments arranged by size:
2878 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5037 5435 5709 5856 6267 5037
Size: 398 274 147 411 5374
Fragments arranged by size:
5374 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2552 2582 1178
Size: 30 165 30 1149 30 5200
Fragments arranged by size:
5200 1149 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3725 1186
Size: 2539 4065
BseYI C'CCAG_C
Cuts at: 2961 3021 5807 6303 2961
Size: 60 2786 496 3262
Fragments arranged by size:
3262 2786 496 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 2392 3005 1163
Size: 573 501 155 613 4762
Fragments arranged by size:
4762 613 573 501 155
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2982 4054 4815 5843 1540
Size: 275 186 981 1072 761 1028 2301
Fragments arranged by size:
2301 1072 1028 981 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2367 2402 2448 3114 3243 3657 3875 4665 4750
Size: 35 46 666 129 414 218 790 85
Cuts at: 4750 5821 2367
Size: 1071 3150
Fragments arranged by size:
3150 1071 790 666 414 218 129 85
46 35
BsmI GAATG_Cn'
Cuts at: 2614 3724 4115 4208 2614
Size: 1110 391 93 5010
Fragments arranged by size:
5010 1110 391 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2210 2256 2367 2402 2448 3114 3243
Size: 399 420 46 111 35 46 666 129
Cuts at: 3243 3300 3657 3875 3923 4665 4750 5821 1391
Size: 57 357 218 48 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 666 420 399 357 218
129 111 85 57 48 46 46 35
BspEI T'CCGG_A
Cuts at: 6267 6267
Size: 6604
BspHI T'CATG_A
Cuts at: 3685 4493 6223 3685
Size: 808 1730 4066
Fragments arranged by size:
4066 1730 808
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3959 844
Size: 3115 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4491 1394
Size: 429 2668 3507
Fragments arranged by size:
3507 2668 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3093 5099 5273 65
Size: 1183 1845 2006 174 1396
Fragments arranged by size:
2006 1845 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2715 5248 5435 1700
Size: 110 905 2533 187 2869
Fragments arranged by size:
2869 2533 905 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6604
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6604
BssSI C'ACGA_G
Cuts at: 4664 5676 4664
Size: 1012 5592
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6604
BstBI TT'CG_AA
Cuts at: 5408 5408
Size: 6604
BstEII G'GTnAC_C
Cuts at: 1184 2324 2576 1184
Size: 1140 252 5212
Fragments arranged by size:
5212 1140 252
BstXI CCAn_nnnn'nTGG
Cuts at: 2026 2026
Size: 6604
BstYI r'GATC_y
Cuts at: 1972 2346 2776 3934 4301 4686 4703 6155 6241
Size: 374 430 1158 367 385 17 1452 86
Cuts at: 6241 6253 6599 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1158 430 385 374 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2846 2846
Size: 6604
BtgI C'CryG_G
Cuts at: 513 1065 2004 2211 513
Size: 552 939 207 4906
Fragments arranged by size:
4906 939 552 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2325 487
Size: 683 56 1099 4766
Fragments arranged by size:
4766 1099 683 56
BtsI GCAGTG_nn'
Cuts at: 2898 2931 2952 3477 4120 4914 4934 2898
Size: 33 21 525 643 794 20 4568
Fragments arranged by size:
4568 794 643 525 33 21 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6604
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2491 2526 529
Size: 35 1927 35 4607
Fragments arranged by size:
4607 1927 35 35
DraI TTT'AAA
Cuts at: 3519 3739 3883 3908 3519
Size: 220 144 25 6215
Fragments arranged by size:
6215 220 144 25
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5611 809
Size: 507 4295 1802
Fragments arranged by size:
4295 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2717 2979
Size: 54 1425 50 275 18 297 590 262
Cuts at: 2979 4051 4940 6569 8
Size: 1072 889 1629 43
Fragments arranged by size:
1629 1425 1072 889 590 297 275 262
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2979 4051 1537
Size: 275 1167 1072 4090
Fragments arranged by size:
4090 1167 1072 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3195 4534 1353
Size: 1842 1339 3423
Fragments arranged by size:
3423 1842 1339
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5191 5565 5711 387
Size: 1064 138 3602 374 146 1280
Fragments arranged by size:
3602 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2446 3241 3873 2446
Size: 795 632 5177
Fragments arranged by size:
5177 795 632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2441 2579 2585 2624 2957 3035 3312
Size: 393 809 138 6 39 333 78 277
Cuts at: 3312 3416 3614 4667 5264 6051 6319 1239
Size: 104 198 1053 597 787 268 1524
Fragments arranged by size:
1524 1053 809 787 597 393 333 277
268 198 138 104 78 39 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2476 2849 3082 2476
Size: 373 233 5998
Fragments arranged by size:
5998 373 233
EcoO109I rG'GnC_Cy
Cuts at: 2416 2416
Size: 6604
EcoRI G'AATT_C
Cuts at: 3137 3913 4291 3137
Size: 776 378 5450
Fragments arranged by size:
5450 776 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3916 3916
Size: 6604
FspI TGC'GCA
Cuts at: 5110 5110
Size: 6604
HaeII r_GCGC'y
Cuts at: 1678 5751 1678
Size: 4073 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2158 2190 2744 2776 3541
Size: 32 135 32 586 32 554 32 765
Cuts at: 3541 3573 5245 5277 5319 5351 1373
Size: 32 1672 32 42 32 2626
Fragments arranged by size:
2626 1672 765 586 554 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3948 4194 669
Size: 988 281 2010 246 3079
Fragments arranged by size:
3079 2010 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6604
HpaI GTT'AAC
Cuts at: 4194 4194
Size: 6604
KpnI G_GTAC'C
Cuts at: 3929 3929
Size: 6604
MfeI C'AATT_G
Cuts at: 4203 4203
Size: 6604
MluI A'CGCG_T
Cuts at: 6480 6480
Size: 6604
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3630 5718 5902 1305
Size: 2325 2088 184 2007
Fragments arranged by size:
2325 2088 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2129 10
Size: 54 1425 343 297 4485
Fragments arranged by size:
4485 1425 343 297 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2240 4562 4921 5080 518
Size: 648 1074 2322 359 159 2042
Fragments arranged by size:
2322 2042 1074 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2428 2978 3497 4020 4697 5845 6090 1606
Size: 822 550 519 523 677 1148 245 2120
Fragments arranged by size:
2120 1148 822 677 550 523 519 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5113
Fragments arranged by size:
5113 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6604
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6604
NotI GC'GGCC_GC
Cuts at: 4051 4051
Size: 6604
NspI r_CATG'y
Cuts at: 1132 1229 3074 3962 5507 1132
Size: 97 1845 888 1545 2229
Fragments arranged by size:
2229 1845 1545 888 97
PasI CC'CwG_GG
Cuts at: 1348 2664 1348
Size: 1316 5288
PciI A'CATG_T
Cuts at: 5503 5503
Size: 6604
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2281 2419 3016 1108
Size: 1173 138 597 4696
Fragments arranged by size:
4696 1173 597 138
PfoI T'CCnGG_A
Cuts at: 2342 2787 3051 3289 3393 2342
Size: 445 264 238 104 5553
Fragments arranged by size:
5553 445 264 238 104
PmeI GTTT'AAAC
Cuts at: 3908 3908
Size: 6604
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4673 4705 6186 6218 4673
Size: 32 1481 32 5059
Fragments arranged by size:
5059 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6604
PsiI TTA'TAA
Cuts at: 4174 4174
Size: 6604
PstI C_TGCA'G
Cuts at: 830 3195 3609 3956 830
Size: 2365 414 347 3478
Fragments arranged by size:
3478 2365 414 347
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6604
PvuII CAG'CTG
Cuts at: 2428 3497 2428
Size: 1069 5535
SacI G_AGCT'C
Cuts at: 2448 3243 3875 2448
Size: 795 632 5177
Fragments arranged by size:
5177 795 632
SalI G'TCGA_C
Cuts at: 1936 3946 1936
Size: 2010 4594
SbfI CC_TGCA'GG
Cuts at: 3956 3956
Size: 6604
ScaI AGT'ACT
Cuts at: 4852 6564 4852
Size: 1712 4892
SfcI C'TryA_G
Cuts at: 826 1045 3004 3191 3605 3952 5087 5768 5959
Size: 219 1959 187 414 347 1135 681 191
Cuts at: 5959 826
Size: 1471
Fragments arranged by size:
1959 1471 1135 681 414 347 219 191
187
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6604
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6604
SmaI CCC'GGG
Cuts at: 1872 3931 1872
Size: 2059 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3558 4708 5609 5871 820
Size: 197 926 1615 1150 901 262 1553
Fragments arranged by size:
1615 1553 1150 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4395 493
Size: 3902 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6604
SphI G_CATG'C
Cuts at: 1132 3074 3962 1132
Size: 1942 888 3774
Fragments arranged by size:
3774 1942 888
SspI AAT'ATT
Cuts at: 5 52 2749 3825 4528 5
Size: 47 2697 1076 703 2081
Fragments arranged by size:
2697 2081 1076 703 47
StuI AGG'CCT
Cuts at: 1517 2482 1517
Size: 965 5639
StyI C'CwwG_G
Cuts at: 513 1065 2004 2229 2477 3792 4043 513
Size: 552 939 225 248 1315 251 3074
Fragments arranged by size:
3074 1315 939 552 251 248 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2274 4631 4648 4801 4986 5431 325
Size: 1255 694 2357 17 153 185 445 1498
Fragments arranged by size:
2357 1498 1255 694 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4850 6562 96
Size: 274 80 33 51 1443 2873 1712 138
Fragments arranged by size:
2873 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6564 6596 6564
Size: 32 6572
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6604
XbaI T'CTAG_A
Cuts at: 3940 3940
Size: 6604
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2532 2532
Size: 6604
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6604
XmaI C'CCGG_G
Cuts at: 1870 3929 1870
Size: 2059 4545
XmnI GAAnn'nnTTC
Cuts at: 2702 3149 4733 2702
Size: 447 1584 4573
Fragments arranged by size:
4573 1584 447
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4412 276
Size: 53 83 186 936 2878 2468
Fragments arranged by size:
2878 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI