Restriction map for
pFN21AE0332
(Circular) (Six-base) MAPSORT of: pFN21AE0332.seq Check: 1550 from: 1 to: 6583
pFN21AE0332 6583 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2035 2944 2035
Size: 909 5674
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4393 278
Size: 53 83 186 936 2857 2468
Fragments arranged by size:
2857 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3926 1937
Size: 1989 4594
Acc65I G'GTAC_C
Cuts at: 3904 3904
Size: 6583
AclI AA'CG_TT
Cuts at: 1563 4710 5083 1563
Size: 3147 373 3063
Fragments arranged by size:
3147 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4646 6030 6298 1632
Size: 3014 1384 268 1917
Fragments arranged by size:
3014 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6386
AflIII A'CryG_T
Cuts at: 1203 2096 3149 5482 6459 1203
Size: 893 1053 2333 977 1327
Fragments arranged by size:
2333 1327 1053 977 893
AgeI A'CCGG_T
Cuts at: 2214 5414 2214
Size: 3200 3383
AhdI GACnn_n'nnGTC
Cuts at: 5312 5312
Size: 6583
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6583
AlwNI CAG_nnn'CTG
Cuts at: 2050 3446 4326 5898 2050
Size: 1396 880 1572 2735
Fragments arranged by size:
2735 1572 1396 880
ApaLI G'TGCA_C
Cuts at: 4640 5796 4640
Size: 1156 5427
ApoI r'AATT_y
Cuts at: 1493 3472 3892 4118 4270 6379 1493
Size: 1979 420 226 152 2109 1697
Fragments arranged by size:
2109 1979 1697 420 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2834 5137 160
Size: 872 1802 2303 1606
Fragments arranged by size:
2303 1802 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
AvaI C'yCGr_G
Cuts at: 1870 1943 3908 1870
Size: 73 1965 4545
Fragments arranged by size:
4545 1965 73
AvrII C'CTAG_G
Cuts at: 2380 2380
Size: 6583
BamHI G'GATC_C
Cuts at: 2076 3913 4280 2076
Size: 1837 367 4379
Fragments arranged by size:
4379 1837 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3904 5359 618
Size: 325 217 627 2117 1455 1842
Fragments arranged by size:
2117 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3902 1391
Size: 2511 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2042 2542 2895 928
Size: 971 143 500 353 4616
Fragments arranged by size:
4616 971 500 353 143
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4774 4808 1903
Size: 34 2837 34 3678
Fragments arranged by size:
3678 2837 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3283 4475 5685 3283
Size: 1192 1210 4181
Fragments arranged by size:
4181 1210 1192
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6583
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5194 136
Size: 107 122 71 631 605 337 3185 1525
Fragments arranged by size:
3185 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6578 6578
Size: 6583
BlpI GC'TnA_GC
Cuts at: 4000 4000
Size: 6583
Bme1580I G_kGCm'C
Cuts at: 1790 4644 5800 1790
Size: 2854 1156 2573
Fragments arranged by size:
2854 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2032 2124 2365 2557 1318
Size: 714 92 241 192 5344
Fragments arranged by size:
5344 714 241 192 92
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3008 4314 5272
Size: 416 53 83 399 24 1591 1306 958
Cuts at: 5272 442
Size: 1753
Fragments arranged by size:
1753 1591 1306 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6583
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3566 3566
Size: 6583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2212 3300 3329 3561 3640 3730 5243 1239
Size: 973 1088 29 232 79 90 1513 2579
Fragments arranged by size:
2579 1513 1088 973 232 90 79 29
Bpu10I CC'TnA_GC
Cuts at: 3084 3084
Size: 6583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2612 3219 4708 5573 5871 2612
Size: 607 1489 865 298 3324
Fragments arranged by size:
3324 1489 865 607 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5246 882
Size: 617 3747 2219
Fragments arranged by size:
3747 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2097 3152 4374 493
Size: 711 893 1055 1222 2702
Fragments arranged by size:
2702 1222 1055 893 711
BsaBI GATnn'nnATC
Cuts at: 6577 6577
Size: 6583
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2019 4390 4772 5407
Size: 53 83 186 936 486 2371 382 635
Cuts at: 5407 275
Size: 1451
Fragments arranged by size:
2371 1451 936 635 486 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2214 5016 5414 5688 5835 6246 2214
Size: 2802 398 274 147 411 2551
Fragments arranged by size:
2802 2551 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2135 2165 2663 2693 3528
Size: 30 165 30 732 30 498 30 835
Cuts at: 3528 3558 3714 3744 1178
Size: 30 156 30 4017
Fragments arranged by size:
4017 835 732 498 165 156 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6583
BseYI C'CCAG_C
Cuts at: 5786 6282 5786
Size: 496 6087
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2063 2157 2568 3756 1163
Size: 573 327 94 411 1188 3990
Fragments arranged by size:
3990 1188 573 411 327 94
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2215 3679 4033 4794 5822 1540
Size: 275 186 214 1464 354 761 1028 2301
Fragments arranged by size:
2301 1464 1028 761 354 275 214 186
BsiHKAI G_wGCw'C
Cuts at: 4644 4729 5800 4644
Size: 85 1071 5427
Fragments arranged by size:
5427 1071 85
BsmI GAATG_Cn'
Cuts at: 3203 3333 4094 4187 3203
Size: 130 761 93 5599
Fragments arranged by size:
5599 761 130 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3902 4644 4729 5800 1391
Size: 399 2112 742 85 1071 2174
Fragments arranged by size:
2174 2112 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6246 6246
Size: 6583
BspHI T'CATG_A
Cuts at: 4472 6202 4472
Size: 1730 4853
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2035 2944 3938 844
Size: 1191 909 994 3489
Fragments arranged by size:
3489 1191 994 909
BsrBI CCG'CTC
Cuts at: 1394 1823 4470 1394
Size: 429 2647 3507
Fragments arranged by size:
3507 2647 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3162 5078 5252 65
Size: 1183 1914 1916 174 1396
Fragments arranged by size:
1916 1914 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2214 5227 5414 1700
Size: 110 404 3013 187 2869
Fragments arranged by size:
3013 2869 404 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6583
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6583
BssSI C'ACGA_G
Cuts at: 2540 4643 5655 2540
Size: 2103 1012 3468
Fragments arranged by size:
3468 2103 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2050 794
Size: 1256 5327
BstBI TT'CG_AA
Cuts at: 3427 5387 3427
Size: 1960 4623
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6583
BstYI r'GATC_y
Cuts at: 1972 2076 3313 3913 4280 4665 4682 6134 6220
Size: 104 1237 600 367 385 17 1452 86
Cuts at: 6220 6232 6578 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1237 600 385 367 346 104
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2306 2306
Size: 6583
BtgI C'CryG_G
Cuts at: 513 1065 3505 513
Size: 552 2440 3591
Fragments arranged by size:
3591 2440 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5844
Fragments arranged by size:
5844 683 56
BtsI GCAGTG_nn'
Cuts at: 2140 3786 4099 4893 4913 2140
Size: 1646 313 794 20 3810
Fragments arranged by size:
3810 1646 794 313 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6583
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6548
DraI TTT'AAA
Cuts at: 3887 3887
Size: 6583
DraIII CAC_nnn'GTG
Cuts at: 2127 2127
Size: 6583
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5590 809
Size: 507 4274 1802
Fragments arranged by size:
4274 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2113 2659
Size: 54 1425 50 275 18 180 103 546
Cuts at: 2659 3484 3676 4030 4919 6548 8
Size: 825 192 354 889 1629 43
Fragments arranged by size:
1629 1425 889 825 546 354 275 192
180 103 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3676 4030 1537
Size: 275 1864 354 4090
Fragments arranged by size:
4090 1864 354 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 4513 1353
Size: 812 2348 3423
Fragments arranged by size:
3423 2348 812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3491 5170 5544 5690 387
Size: 1064 138 1902 1679 374 146 1280
Fragments arranged by size:
1902 1679 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2212 3300 3329 3561 3640 3730 4646
Size: 393 580 1088 29 232 79 90 916
Cuts at: 4646 5243 6030 6298 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1088 916 787 597 580 393 268
232 90 79 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2384 2435 2384
Size: 51 6532
EcoO109I rG'GnC_Cy
Cuts at: 2376 2376
Size: 6583
EcoRI G'AATT_C
Cuts at: 3892 4270 3892
Size: 378 6205
EcoRV GAT'ATC
Cuts at: 3760 3760
Size: 6583
FspI TGC'GCA
Cuts at: 5089 5089
Size: 6583
HaeII r_GCGC'y
Cuts at: 1678 5730 1678
Size: 4052 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 2462 2494 2950
Size: 32 135 32 563 32 295 32 456
Cuts at: 2950 2982 3278 3310 3356 3388 5224 5256 5298
Size: 32 296 32 46 32 1836 32 42
Cuts at: 5298 5330 1373
Size: 32 2626
Fragments arranged by size:
2626 1836 563 456 296 295 135 46
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2362 3927 4173 669
Size: 988 281 424 1565 246 3079
Fragments arranged by size:
3079 1565 988 424 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6583
HpaI GTT'AAC
Cuts at: 4173 4173
Size: 6583
KpnI G_GTAC'C
Cuts at: 3908 3908
Size: 6583
MfeI C'AATT_G
Cuts at: 2823 3340 4182 2823
Size: 517 842 5224
Fragments arranged by size:
5224 842 517
MluI A'CGCG_T
Cuts at: 6459 6459
Size: 6583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5697 5881 1305
Size: 4392 184 2007
Fragments arranged by size:
4392 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2012 2115 2661 3486 10
Size: 54 1425 343 180 103 546 825 3107
Fragments arranged by size:
3107 1425 825 546 343 180 103 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4541 4900 5059 518
Size: 648 3375 359 159 2042
Fragments arranged by size:
3375 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2141 3740 3999 4676 5824 6069 1606
Size: 535 1599 259 677 1148 245 2120
Fragments arranged by size:
2120 1599 1148 677 535 259 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6031
NdeI CA'TA_TG
Cuts at: 387 2977 387
Size: 2590 3993
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6583
NotI GC'GGCC_GC
Cuts at: 4030 4030
Size: 6583
NspI r_CATG'y
Cuts at: 1132 1229 2240 2406 3386 3941 5486 1132
Size: 97 1011 166 980 555 1545 2229
Fragments arranged by size:
2229 1545 1011 980 555 166 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6583
PciI A'CATG_T
Cuts at: 5482 5482
Size: 6583
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2668 1108
Size: 1560 5023
PmeI GTTT'AAAC
Cuts at: 3887 3887
Size: 6583
PmlI CAC'GTG
Cuts at: 2097 3152 2097
Size: 1055 5528
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3009 3041 4652 4684 6165 6197 3009
Size: 32 1611 32 1481 32 3395
Fragments arranged by size:
3395 1611 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2376 2376
Size: 6583
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6583
PsiI TTA'TAA
Cuts at: 4153 4153
Size: 6583
PstI C_TGCA'G
Cuts at: 830 2135 3451 3935 830
Size: 1305 1316 484 3478
Fragments arranged by size:
3478 1316 1305 484
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6583
PvuII CAG'CTG
Cuts at: 3740 3740
Size: 6583
SalI G'TCGA_C
Cuts at: 1936 3925 1936
Size: 1989 4594
SanDI GG'GwC_CC
Cuts at: 2376 2376
Size: 6583
SbfI CC_TGCA'GG
Cuts at: 3935 3935
Size: 6583
ScaI AGT'ACT
Cuts at: 4831 6543 4831
Size: 1712 4871
SfcI C'TryA_G
Cuts at: 826 1045 2131 2727 3447 3703 3931 5066 5747
Size: 219 1086 596 720 256 228 1135 681
Cuts at: 5747 5938 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1086 720 681 596 256 228
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6583
SmaI CCC'GGG
Cuts at: 1872 3910 1872
Size: 2038 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2591 3234 4687 5588 5850 820
Size: 197 926 648 643 1453 901 262 1553
Fragments arranged by size:
1553 1453 926 901 648 643 262 197
SnaBI TAC'GTA
Cuts at: 493 4374 493
Size: 3881 2702
SpeI A'CTAG_T
Cuts at: 152 3809 152
Size: 3657 2926
SphI G_CATG'C
Cuts at: 1132 3386 3941 1132
Size: 2254 555 3774
Fragments arranged by size:
3774 2254 555
SspI AAT'ATT
Cuts at: 5 52 2767 4507 5
Size: 47 2715 1740 2081
Fragments arranged by size:
2715 2081 1740 47
StuI AGG'CCT
Cuts at: 1517 2675 1517
Size: 1158 5425
StyI C'CwwG_G
Cuts at: 513 1065 2380 3408 3492 4022 513
Size: 552 1315 1028 84 530 3074
Fragments arranged by size:
3074 1315 1028 552 530 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2254 2264 4610 4627 4780 4965 5410
Size: 1255 674 10 2346 17 153 185 445
Cuts at: 5410 325
Size: 1498
Fragments arranged by size:
2346 1498 1255 674 445 185 153 17
10
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3344 4829 6541
Size: 274 80 33 51 1443 1367 1485 1712
Cuts at: 6541 96
Size: 138
Fragments arranged by size:
1712 1485 1443 1367 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2663 2695 2784 2816 3796 3828 6543 6575 2663
Size: 32 89 32 980 32 2715 32 2671
Fragments arranged by size:
2715 2671 980 89 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6583
XbaI T'CTAG_A
Cuts at: 3919 3919
Size: 6583
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3631 3631
Size: 6583
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6583
XmaI C'CCGG_G
Cuts at: 1870 3908 1870
Size: 2038 4545
XmnI GAAnn'nnTTC
Cuts at: 4712 4712
Size: 6583
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4391 276
Size: 53 83 186 936 2857 2468
Fragments arranged by size:
2857 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I EcoICRI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI