Restriction map for
pFN21AE0330
(Circular) (Six-base) MAPSORT of: pFN21AE0330.seq Check: 9425 from: 1 to: 6529
pFN21AE0330 6529 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4339 278
Size: 53 83 186 936 2803 2468
Fragments arranged by size:
2803 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3872 1937
Size: 1935 4594
Acc65I G'GTAC_C
Cuts at: 3850 3850
Size: 6529
AclI AA'CG_TT
Cuts at: 1563 4656 5029 1563
Size: 3093 373 3063
Fragments arranged by size:
3093 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2814 3098 3260 3340 4592 5976 6244 1632
Size: 1182 284 162 80 1252 1384 268 1917
Fragments arranged by size:
1917 1384 1252 1182 284 268 162 80
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6332
AflIII A'CryG_T
Cuts at: 1203 5428 6405 1203
Size: 4225 977 1327
Fragments arranged by size:
4225 1327 977
AgeI A'CCGG_T
Cuts at: 5360 5360
Size: 6529
AhdI GACnn_n'nnGTC
Cuts at: 3297 5258 3297
Size: 1961 4568
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6529
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6529
AlwNI CAG_nnn'CTG
Cuts at: 2263 2295 4272 5844 2263
Size: 32 1977 1572 2948
Fragments arranged by size:
2948 1977 1572 32
ApaI G_GGCC'C
Cuts at: 2116 2116
Size: 6529
ApaLI G'TGCA_C
Cuts at: 4586 5742 4586
Size: 1156 5373
ApoI r'AATT_y
Cuts at: 1493 2579 3305 3838 4064 4216 6325 1493
Size: 1086 726 533 226 152 2109 1697
Fragments arranged by size:
2109 1697 1086 726 533 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5083 160
Size: 872 4051 1606
Fragments arranged by size:
4051 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6529
AvaI C'yCGr_G
Cuts at: 1870 1943 2049 2206 3854 1870
Size: 73 106 157 1648 4545
Fragments arranged by size:
4545 1648 157 106 73
AvrII C'CTAG_G
Cuts at: 2584 2584
Size: 6529
BamHI G'GATC_C
Cuts at: 2030 3859 4226 2030
Size: 1829 367 4333
Fragments arranged by size:
4333 1829 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2151 3523 3684 3850 5305
Size: 325 217 627 364 1372 161 166 1455
Cuts at: 5305 618
Size: 1842
Fragments arranged by size:
1842 1455 1372 627 364 325 217 166
161
BanII G_rGCy'C
Cuts at: 1391 2116 2229 2454 2792 3318 3402 3848 1391
Size: 725 113 225 338 526 84 446 4072
Fragments arranged by size:
4072 725 526 446 338 225 113 84
BbeI G_GCGC'C
Cuts at: 2155 2155
Size: 6529
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2589 928
Size: 971 690 4868
Fragments arranged by size:
4868 971 690
BbvCI CC'TCA_GC
Cuts at: 3189 3693 3189
Size: 504 6025
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4720 4754 1903
Size: 34 50 34 2699 34 3678
Fragments arranged by size:
3678 2699 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4421 5631 4421
Size: 1210 5319
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6529
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2043 3065 5140
Size: 107 122 71 631 605 371 1022 2075
Cuts at: 5140 136
Size: 1525
Fragments arranged by size:
2075 1525 1022 631 605 371 122 107
71
BglII A'GATC_T
Cuts at: 2590 6524 2590
Size: 3934 2595
BlpI GC'TnA_GC
Cuts at: 2363 3647 3946 2363
Size: 1284 299 4946
Fragments arranged by size:
4946 1284 299
Bme1580I G_kGCm'C
Cuts at: 1790 2116 3553 4590 5746 1790
Size: 326 1437 1037 1156 2573
Fragments arranged by size:
2573 1437 1156 1037 326
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6529
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3547 4260 5218
Size: 416 53 83 399 24 2130 713 958
Cuts at: 5218 442
Size: 1753
Fragments arranged by size:
2130 1753 958 713 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2933 3332 5189 1239
Size: 1694 399 1857 2579
Fragments arranged by size:
2579 1857 1694 399
Bpu10I CC'TnA_GC
Cuts at: 3189 3693 3189
Size: 504 6025
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2377 3054 4654 5519 5817 2377
Size: 677 1600 865 298 3089
Fragments arranged by size:
3089 1600 865 677 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5192 882
Size: 617 3693 2219
Fragments arranged by size:
3693 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4320 493
Size: 711 3116 2702
Fragments arranged by size:
3116 2702 711
BsaBI GATnn'nnATC
Cuts at: 6523 6523
Size: 6529
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2152 2241 4336 4718
Size: 53 83 186 936 619 89 2095 382
Cuts at: 4718 5353 275
Size: 635 1451
Fragments arranged by size:
2095 1451 936 635 619 382 186 89
83 53
BsaWI w'CCGG_w
Cuts at: 4962 5360 5634 5781 6192 4962
Size: 398 274 147 411 5299
Fragments arranged by size:
5299 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6304
Fragments arranged by size:
6304 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 3206 3533 1186
Size: 943 1077 327 4182
Fragments arranged by size:
4182 1077 943 327
BseYI C'CCAG_C
Cuts at: 3725 5732 6228 3725
Size: 2007 496 4026
Fragments arranged by size:
4026 2007 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3771 1163
Size: 573 2035 3921
Fragments arranged by size:
3921 2035 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2107 3979 4740 5768 1540
Size: 275 186 106 1872 761 1028 2301
Fragments arranged by size:
2301 1872 1028 761 275 186 106
BsiHKAI G_wGCw'C
Cuts at: 3318 3402 3648 4590 4675 5746 3318
Size: 84 246 942 85 1071 4101
Fragments arranged by size:
4101 1071 942 246 85 84
BsmI GAATG_Cn'
Cuts at: 2707 2933 3207 3711 4040 4133 2707
Size: 226 274 504 329 93 5103
Fragments arranged by size:
5103 504 329 274 226 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2116 2229 2454 2792 3318 3402 3553
Size: 399 326 113 225 338 526 84 151
Cuts at: 3553 3648 3848 4590 4675 5746 1391
Size: 95 200 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 526 399 338 326 225
200 151 113 95 85 84
BspEI T'CCGG_A
Cuts at: 6192 6192
Size: 6529
BspHI T'CATG_A
Cuts at: 3253 4418 6148 3253
Size: 1165 1730 3634
Fragments arranged by size:
3634 1730 1165
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2158 2903 3884 844
Size: 1314 745 981 3489
Fragments arranged by size:
3489 1314 981 745
BsrBI CCG'CTC
Cuts at: 1394 1823 2054 2205 2494 3043 4416 1394
Size: 429 231 151 289 549 1373 3507
Fragments arranged by size:
3507 1373 549 429 289 231 151
BsrDI GCAATG_nn'
Cuts at: 65 1248 5024 5198 65
Size: 1183 3776 174 1396
Fragments arranged by size:
3776 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2042 2102 5173 5360 1700
Size: 110 232 60 3071 187 2869
Fragments arranged by size:
3071 2869 232 187 110 60
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6529
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6529
BssSI C'ACGA_G
Cuts at: 4589 5601 4589
Size: 1012 5517
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2263 794
Size: 1469 5060
BstBI TT'CG_AA
Cuts at: 5333 5333
Size: 6529
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6529
BstXI CCAn_nnnn'nTGG
Cuts at: 3131 3131
Size: 6529
BstYI r'GATC_y
Cuts at: 1972 2030 2292 2590 3859 4226 4611 4628 6080
Size: 58 262 298 1269 367 385 17 1452
Cuts at: 6080 6166 6178 6524 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1269 385 367 346 298 262
86 58 17 12
Bsu36I CC'TnA_GG
Cuts at: 3233 3755 3233
Size: 522 6007
BtgI C'CryG_G
Cuts at: 513 1065 2072 2107 2189 3054 513
Size: 552 1007 35 82 865 3988
Fragments arranged by size:
3988 1007 865 552 82 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2734 487
Size: 683 56 1508 4282
Fragments arranged by size:
4282 1508 683 56
BtsI GCAGTG_nn'
Cuts at: 3113 3155 4045 4839 4859 3113
Size: 42 890 794 20 4783
Fragments arranged by size:
4783 890 794 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6529
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6494
DraI TTT'AAA
Cuts at: 3833 3833
Size: 6529
DraIII CAC_nnn'GTG
Cuts at: 2907 3142 2907
Size: 235 6294
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5536 809
Size: 507 4220 1802
Fragments arranged by size:
4220 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2104 3057
Size: 54 1425 50 275 18 210 64 953
Cuts at: 3057 3976 4865 6494 8
Size: 919 889 1629 43
Fragments arranged by size:
1629 1425 953 919 889 275 210 64
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2104 3976 1537
Size: 275 292 1872 4090
Fragments arranged by size:
4090 1872 292 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4459 1353
Size: 3106 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3572 5116 5490 5636 387
Size: 1064 138 1983 1544 374 146 1280
Fragments arranged by size:
1983 1544 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3316 3400 3316
Size: 84 6445
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2814 2933 3098 3260 3332 3340 4592
Size: 393 1182 119 165 162 72 8 1252
Cuts at: 4592 5189 5976 6244 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1252 1182 787 597 393 268 165
162 119 72 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2851 3692 2851
Size: 841 5688
EcoO109I rG'GnC_Cy
Cuts at: 2112 2113 2170 2488 2856 2892 3169 2112
Size: 1 57 318 368 36 277 5472
Fragments arranged by size:
5472 368 318 277 57 36 1
EcoRI G'AATT_C
Cuts at: 3305 3838 4216 3305
Size: 533 378 5618
Fragments arranged by size:
5618 533 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3401 3401
Size: 6529
FspI TGC'GCA
Cuts at: 5035 5035
Size: 6529
HaeII r_GCGC'y
Cuts at: 1678 2155 5676 1678
Size: 477 3521 2531
Fragments arranged by size:
3521 2531 477
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2189 2221 2284 2316 2441
Size: 32 135 32 617 32 63 32 125
Cuts at: 2441 2473 3283 3315 5170 5202 5244 5276 1373
Size: 32 810 32 1855 32 42 32 2626
Fragments arranged by size:
2626 1855 810 617 135 125 63 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3873 4119 669
Size: 988 281 1935 246 3079
Fragments arranged by size:
3079 1935 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2610 3107 748
Size: 1862 497 4170
Fragments arranged by size:
4170 1862 497
HpaI GTT'AAC
Cuts at: 4119 4119
Size: 6529
KasI G'GCGC_C
Cuts at: 2151 2151
Size: 6529
KpnI G_GTAC'C
Cuts at: 3854 3854
Size: 6529
MfeI C'AATT_G
Cuts at: 4128 4128
Size: 6529
MluI A'CGCG_T
Cuts at: 6405 6405
Size: 6529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5643 5827 1305
Size: 4338 184 2007
Fragments arranged by size:
4338 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4707
Fragments arranged by size:
4707 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4487 4846 5005 518
Size: 648 3321 359 159 2042
Fragments arranged by size:
3321 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2074 2109 2191 3945 4622 5770 6015 1606
Size: 468 35 82 1754 677 1148 245 2120
Fragments arranged by size:
2120 1754 1148 677 468 245 82 35
NaeI GCC'GGC
Cuts at: 2044 2104 2044
Size: 60 6469
NarI GG'CG_CC
Cuts at: 2152 2152
Size: 6529
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5977
NdeI CA'TA_TG
Cuts at: 387 3805 387
Size: 3418 3111
NgoMIV G'CCGG_C
Cuts at: 2042 2102 2042
Size: 60 6469
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6529
NotI GC'GGCC_GC
Cuts at: 3976 3976
Size: 6529
NspI r_CATG'y
Cuts at: 1132 1229 3887 5432 1132
Size: 97 2658 1545 2229
Fragments arranged by size:
2658 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6529
PciI A'CATG_T
Cuts at: 5428 5428
Size: 6529
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2956 1108
Size: 1848 4681
PfoI T'CCnGG_A
Cuts at: 3309 3309
Size: 6529
PmeI GTTT'AAAC
Cuts at: 3833 3833
Size: 6529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4598 4630 6111 6143 4598
Size: 32 1481 32 4984
Fragments arranged by size:
4984 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2170 2488 2856 2892 3169 2170
Size: 318 368 36 277 5530
Fragments arranged by size:
5530 368 318 277 36
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6529
PsiI TTA'TAA
Cuts at: 3413 4099 3413
Size: 686 5843
PspOMI G'GGCC_C
Cuts at: 2112 2112
Size: 6529
PstI C_TGCA'G
Cuts at: 830 3202 3768 3881 830
Size: 2372 566 113 3478
Fragments arranged by size:
3478 2372 566 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6529
SacI G_AGCT'C
Cuts at: 3318 3402 3318
Size: 84 6445
SacII CC_GC'GG
Cuts at: 2075 2110 2192 2075
Size: 35 82 6412
Fragments arranged by size:
6412 82 35
SalI G'TCGA_C
Cuts at: 1936 3871 1936
Size: 1935 4594
SanDI GG'GwC_CC
Cuts at: 3169 3169
Size: 6529
SbfI CC_TGCA'GG
Cuts at: 3881 3881
Size: 6529
ScaI AGT'ACT
Cuts at: 4777 6489 4777
Size: 1712 4817
SfcI C'TryA_G
Cuts at: 826 1045 2296 3198 3764 3877 5012 5693 5884
Size: 219 1251 902 566 113 1135 681 191
Cuts at: 5884 826
Size: 1471
Fragments arranged by size:
1471 1251 1135 902 681 566 219 191
113
SfoI GGC'GCC
Cuts at: 2153 2153
Size: 6529
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6529
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6529
SmaI CCC'GGG
Cuts at: 1872 3856 1872
Size: 1984 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2049 2392 3069 4633 5534 5796
Size: 197 926 106 343 677 1564 901 262
Cuts at: 5796 820
Size: 1553
Fragments arranged by size:
1564 1553 926 901 677 343 262 197
106
SnaBI TAC'GTA
Cuts at: 493 4320 493
Size: 3827 2702
SpeI A'CTAG_T
Cuts at: 152 2060 152
Size: 1908 4621
SphI G_CATG'C
Cuts at: 1132 3887 1132
Size: 2755 3774
SspI AAT'ATT
Cuts at: 5 52 4453 5
Size: 47 4401 2081
Fragments arranged by size:
4401 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6529
StyI C'CwwG_G
Cuts at: 513 1065 2584 3968 513
Size: 552 1519 1384 3074
Fragments arranged by size:
3074 1519 1384 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4556 4573 4726 4911 5356 325
Size: 1255 2976 17 153 185 445 1498
Fragments arranged by size:
2976 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2683 3630 4775
Size: 274 80 33 51 1443 706 947 1145
Cuts at: 4775 6487 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1145 947 706 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3105 3126 3137 3158 6489 6521 3105
Size: 21 11 21 3331 32 3113
Fragments arranged by size:
3331 3113 32 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6529
XbaI T'CTAG_A
Cuts at: 3865 3865
Size: 6529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3061 3061
Size: 6529
XhoI C'TCGA_G
Cuts at: 1943 2049 1943
Size: 106 6423
XmaI C'CCGG_G
Cuts at: 1870 3854 1870
Size: 1984 4545
XmnI GAAnn'nnTTC
Cuts at: 2540 3381 4658 2540
Size: 841 1277 4411
Fragments arranged by size:
4411 1277 841
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4337 276
Size: 53 83 186 936 2803 2468
Fragments arranged by size:
2803 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI PvuII RsrII SapI SexAI SfiI
SrfI SwaI