Restriction map for
pFN21AE0327
(Circular) (Six-base) MAPSORT of: pFN21AE0327.seq Check: 7578 from: 1 to: 6559
pFN21AE0327 6559 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4369 278
Size: 53 83 186 936 2833 2468
Fragments arranged by size:
2833 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3788 3902 1937
Size: 1851 114 4594
Fragments arranged by size:
4594 1851 114
Acc65I G'GTAC_C
Cuts at: 3880 3880
Size: 6559
AclI AA'CG_TT
Cuts at: 1563 4686 5059 1563
Size: 3123 373 3063
Fragments arranged by size:
3123 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 2193 3158 3470 4622 6006 6274 1632
Size: 422 139 965 312 1152 1384 268 1917
Fragments arranged by size:
1917 1384 1152 965 422 312 268 139
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6362
AflIII A'CryG_T
Cuts at: 1203 5458 6435 1203
Size: 4255 977 1327
Fragments arranged by size:
4255 1327 977
AgeI A'CCGG_T
Cuts at: 5390 5390
Size: 6559
AhdI GACnn_n'nnGTC
Cuts at: 5288 5288
Size: 6559
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6559
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6559
AlwNI CAG_nnn'CTG
Cuts at: 2032 4302 5874 2032
Size: 2270 1572 2717
Fragments arranged by size:
2717 2270 1572
ApaI G_GGCC'C
Cuts at: 3342 3342
Size: 6559
ApaLI G'TGCA_C
Cuts at: 4616 5772 4616
Size: 1156 5403
ApoI r'AATT_y
Cuts at: 1493 2391 2941 3028 3359 3868 4094 4246 6355
Size: 898 550 87 331 509 226 152 2109
Cuts at: 6355 1493
Size: 1697
Fragments arranged by size:
2109 1697 898 550 509 331 226 152
87
AseI AT'TA_AT
Cuts at: 160 1032 2928 5113 160
Size: 872 1896 2185 1606
Fragments arranged by size:
2185 1896 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6559
AvaI C'yCGr_G
Cuts at: 1870 1943 3663 3884 1870
Size: 73 1720 221 4545
Fragments arranged by size:
4545 1720 221 73
BamHI G'GATC_C
Cuts at: 3889 4256 3889
Size: 367 6192
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2167 2794 3880 5335 618
Size: 325 217 627 380 627 1086 1455 1842
Fragments arranged by size:
1842 1455 1086 627 627 380 325 217
BanII G_rGCy'C
Cuts at: 1391 2023 3294 3342 3878 1391
Size: 632 1271 48 536 4072
Fragments arranged by size:
4072 1271 632 536 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2429 3271 928
Size: 971 530 842 4216
Fragments arranged by size:
4216 971 842 530
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2495 2529 4750 4784 1903
Size: 34 558 34 2221 34 3678
Fragments arranged by size:
3678 2221 558 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2053 3141 3363 4451 5661 2053
Size: 1088 222 1088 1210 2951
Fragments arranged by size:
2951 1210 1088 1088 222
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6559
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2037 5170 136
Size: 107 122 71 631 605 365 3133 1525
Fragments arranged by size:
3133 1525 631 605 365 122 107 71
BglII A'GATC_T
Cuts at: 3737 6554 3737
Size: 2817 3742
BlpI GC'TnA_GC
Cuts at: 3976 3976
Size: 6559
Bme1580I G_kGCm'C
Cuts at: 1790 2170 3342 4620 5776 1790
Size: 380 1172 1278 1156 2573
Fragments arranged by size:
2573 1278 1172 1156 380
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6559
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2275 4290 5248
Size: 416 53 83 399 24 858 2015 958
Cuts at: 5248 442
Size: 1753
Fragments arranged by size:
2015 1753 958 858 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2528 1056
Size: 1472 5087
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3254 5219 1239
Size: 2015 1965 2579
Fragments arranged by size:
2579 2015 1965
Bpu10I CC'TnA_GC
Cuts at: 2228 2228
Size: 6559
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4684 5549 5847 4684
Size: 865 298 5396
Fragments arranged by size:
5396 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5222 882
Size: 617 3723 2219
Fragments arranged by size:
3723 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2509 3799 4350 493
Size: 711 1305 1290 551 2702
Fragments arranged by size:
2702 1305 1290 711 551
BsaBI GATnn'nnATC
Cuts at: 3351 6553 3351
Size: 3202 3357
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4366 4748 5383 275
Size: 53 83 186 936 2833 382 635 1451
Fragments arranged by size:
2833 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2127 4992 5390 5664 5811 6222 2127
Size: 2865 398 274 147 411 2464
Fragments arranged by size:
2865 2464 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3043 3073 1178
Size: 30 165 30 1640 30 4664
Fragments arranged by size:
4664 1640 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2868 3122 1186
Size: 1682 254 4623
Fragments arranged by size:
4623 1682 254
BseYI C'CCAG_C
Cuts at: 2095 2323 2653 2666 3452 5762 6258 2095
Size: 228 330 13 786 2310 496 2396
Fragments arranged by size:
2396 2310 786 496 330 228 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2715 3153 1163
Size: 573 979 438 4569
Fragments arranged by size:
4569 979 573 438
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4009 4770 5798 1540
Size: 275 186 2008 761 1028 2301
Fragments arranged by size:
2301 2008 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2127 3240 4620 4705 5776 2127
Size: 1113 1380 85 1071 2910
Fragments arranged by size:
2910 1380 1113 1071 85
BsmI GAATG_Cn'
Cuts at: 2570 3758 4070 4163 2570
Size: 1188 312 93 4966
Fragments arranged by size:
4966 1188 312 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2127 2170 3240 3294 3342 3878
Size: 399 233 104 43 1070 54 48 536
Cuts at: 3878 4620 4705 5776 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1070 742 536 399 233 104
85 54 48 43
BspEI T'CCGG_A
Cuts at: 6222 6222
Size: 6559
BspHI T'CATG_A
Cuts at: 2733 4448 6178 2733
Size: 1715 1730 3114
Fragments arranged by size:
3114 1730 1715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3012 3914 844
Size: 2168 902 3489
Fragments arranged by size:
3489 2168 902
BsrBI CCG'CTC
Cuts at: 1394 1823 4446 1394
Size: 429 2623 3507
Fragments arranged by size:
3507 2623 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2643 2712 5054 5228 65
Size: 1183 1395 69 2342 174 1396
Fragments arranged by size:
2342 1396 1395 1183 174 69
BsrFI r'CCGG_y
Cuts at: 1700 1810 5203 5390 1700
Size: 110 3393 187 2869
Fragments arranged by size:
3393 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2595 96
Size: 2499 4060
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6559
BssSI C'ACGA_G
Cuts at: 4619 5631 4619
Size: 1012 5547
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2384 2556 794
Size: 1590 172 4797
Fragments arranged by size:
4797 1590 172
BstBI TT'CG_AA
Cuts at: 5363 5363
Size: 6559
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6559
BstYI r'GATC_y
Cuts at: 1972 2177 3737 3889 4256 4641 4658 6110 6196
Size: 205 1560 152 367 385 17 1452 86
Cuts at: 6196 6208 6554 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1560 1452 385 367 346 205 152
86 17 12
BstZ17I GTA'TAC
Cuts at: 3789 3789
Size: 6559
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5068
Fragments arranged by size:
5068 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2446 487
Size: 683 56 1220 4600
Fragments arranged by size:
4600 1220 683 56
BtsI GCAGTG_nn'
Cuts at: 2059 4075 4869 4889 2059
Size: 2016 794 20 3729
Fragments arranged by size:
3729 2016 794 20
ClaI AT'CG_AT
Cuts at: 1554 3445 1554
Size: 1891 4668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3026 3061 3538 3573 529
Size: 35 2462 35 477 35 3515
Fragments arranged by size:
3515 2462 477 35 35 35
DraI TTT'AAA
Cuts at: 3863 3863
Size: 6559
DraIII CAC_nnn'GTG
Cuts at: 3214 3214
Size: 6559
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5566 809
Size: 507 4250 1802
Fragments arranged by size:
4250 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2369 2684 3546
Size: 54 1425 50 275 18 539 315 862
Cuts at: 3546 4006 4895 6524 8
Size: 460 889 1629 43
Fragments arranged by size:
1629 1425 889 862 539 460 315 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4006 1537
Size: 275 2194 4090
Fragments arranged by size:
4090 2194 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2744 3585 4489 1353
Size: 1391 841 904 3423
Fragments arranged by size:
3423 1391 904 841
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5146 5520 5666 387
Size: 1064 138 3557 374 146 1280
Fragments arranged by size:
3557 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2193 3158 3254 3470 4622 5219
Size: 393 422 139 965 96 216 1152 597
Cuts at: 5219 6006 6274 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1152 965 787 597 422 393 268
216 139 96
EcoO109I rG'GnC_Cy
Cuts at: 3339 3539 3339
Size: 200 6359
EcoRI G'AATT_C
Cuts at: 2391 3868 4246 2391
Size: 1477 378 4704
Fragments arranged by size:
4704 1477 378
EcoRV GAT'ATC
Cuts at: 3227 3332 3613 3227
Size: 105 281 6173
Fragments arranged by size:
6173 281 105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2773 2773
Size: 6559
FspI TGC'GCA
Cuts at: 5065 5065
Size: 6559
HaeII r_GCGC'y
Cuts at: 1678 2032 3156 5706 1678
Size: 354 1124 2550 2531
Fragments arranged by size:
2550 2531 1124 354
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5200 5232 5274 5306 1373
Size: 32 135 32 3628 32 42 32 2626
Fragments arranged by size:
3628 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3903 4149 669
Size: 988 281 1965 246 3079
Fragments arranged by size:
3079 1965 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6559
HpaI GTT'AAC
Cuts at: 4149 4149
Size: 6559
KpnI G_GTAC'C
Cuts at: 3884 3884
Size: 6559
MfeI C'AATT_G
Cuts at: 3475 4158 3475
Size: 683 5876
MluI A'CGCG_T
Cuts at: 6435 6435
Size: 6559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5673 5857 1305
Size: 4368 184 2007
Fragments arranged by size:
4368 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2371 2686 3548 10
Size: 54 1425 343 539 315 862 3021
Fragments arranged by size:
3021 1425 862 539 343 315 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2549 2648 3139 4517 4876 5035 518
Size: 648 1383 99 491 1378 359 159 2042
Fragments arranged by size:
2042 1383 1378 648 491 359 159 99
MspA1I CmG'CkG
Cuts at: 1606 2043 2375 2666 3975 4652 5800 6045 1606
Size: 437 332 291 1309 677 1148 245 2120
Fragments arranged by size:
2120 1309 1148 677 437 332 291 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5068
Fragments arranged by size:
5068 939 552
NdeI CA'TA_TG
Cuts at: 387 2329 387
Size: 1942 4617
NheI G'CTAG_C
Cuts at: 1052 2524 1052
Size: 1472 5087
NotI GC'GGCC_GC
Cuts at: 4006 4006
Size: 6559
NspI r_CATG'y
Cuts at: 1132 1229 2554 3917 5462 1132
Size: 97 1325 1363 1545 2229
Fragments arranged by size:
2229 1545 1363 1325 97
PacI TTA_AT'TAA
Cuts at: 2928 2928
Size: 6559
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6559
PciI A'CATG_T
Cuts at: 5458 5458
Size: 6559
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6559
PmeI GTTT'AAAC
Cuts at: 3863 3863
Size: 6559
PmlI CAC'GTG
Cuts at: 2509 2509
Size: 6559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4628 4660 6141 6173 4628
Size: 32 1481 32 5014
Fragments arranged by size:
5014 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3539 3539
Size: 6559
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6559
PsiI TTA'TAA
Cuts at: 3080 4129 3080
Size: 1049 5510
PspOMI G'GGCC_C
Cuts at: 3338 3338
Size: 6559
PstI C_TGCA'G
Cuts at: 830 3012 3911 830
Size: 2182 899 3478
Fragments arranged by size:
3478 2182 899
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6559
PvuII CAG'CTG
Cuts at: 2375 2666 2375
Size: 291 6268
RsrII CG'GwC_CG
Cuts at: 2130 2130
Size: 6559
SalI G'TCGA_C
Cuts at: 1936 3901 1936
Size: 1965 4594
SapI GCTCTTCn'nnn_
Cuts at: 3585 3585
Size: 6559
SbfI CC_TGCA'GG
Cuts at: 3911 3911
Size: 6559
ScaI AGT'ACT
Cuts at: 4807 6519 4807
Size: 1712 4847
SfcI C'TryA_G
Cuts at: 826 1045 3008 3375 3712 3907 5042 5723 5914
Size: 219 1963 367 337 195 1135 681 191
Cuts at: 5914 826
Size: 1471
Fragments arranged by size:
1963 1471 1135 681 367 337 219 195
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6559
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6559
SmaI CCC'GGG
Cuts at: 1872 3665 3886 1872
Size: 1793 221 4545
Fragments arranged by size:
4545 1793 221
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4663 5564 5826 820
Size: 197 926 2720 901 262 1553
Fragments arranged by size:
2720 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3799 4350 493
Size: 3306 551 2702
Fragments arranged by size:
3306 2702 551
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6559
SphI G_CATG'C
Cuts at: 1132 3917 1132
Size: 2785 3774
SspI AAT'ATT
Cuts at: 5 52 2318 3677 4483 5
Size: 47 2266 1359 806 2081
Fragments arranged by size:
2266 2081 1359 806 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6559
StyI C'CwwG_G
Cuts at: 513 1065 2004 3054 3998 513
Size: 552 939 1050 944 3074
Fragments arranged by size:
3074 1050 944 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2147 4586 4603 4756 4941 5386 325
Size: 1255 567 2439 17 153 185 445 1498
Fragments arranged by size:
2439 1498 1255 567 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2595 3189 4805
Size: 274 80 33 51 1443 618 594 1616
Cuts at: 4805 6517 96
Size: 1712 138
Fragments arranged by size:
1712 1616 1443 618 594 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3835 3867 6519 6551 3835
Size: 32 2652 32 3843
Fragments arranged by size:
3843 2652 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6559
XbaI T'CTAG_A
Cuts at: 3586 3734 3895 3586
Size: 148 161 6250
Fragments arranged by size:
6250 161 148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2650 3069 2650
Size: 419 6140
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6559
XmaI C'CCGG_G
Cuts at: 1870 3663 3884 1870
Size: 1793 221 4545
Fragments arranged by size:
4545 1793 221
XmnI GAAnn'nnTTC
Cuts at: 2301 2753 4688 2301
Size: 452 1935 4172
Fragments arranged by size:
4172 1935 452
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4367 276
Size: 53 83 186 936 2833 2468
Fragments arranged by size:
2833 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI Bsu36I EcoICRI EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PfoI PspXI PsrI SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI