Restriction map for
pFN21AE0325
(Circular) (Six-base) MAPSORT of: pFN21AE0325.seq Check: 8575 from: 1 to: 6217
pFN21AE0325 6217 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2358 2358
Size: 6217
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3096 4027 278
Size: 53 83 186 936 1560 931 2468
Fragments arranged by size:
2468 1560 936 931 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3560 1937
Size: 1623 4594
Acc65I G'GTAC_C
Cuts at: 3538 3538
Size: 6217
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2746 2802 4280 5664 5932 1632
Size: 1114 56 1478 1384 268 1917
Fragments arranged by size:
1917 1478 1384 1114 268 56
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6020
AflIII A'CryG_T
Cuts at: 1203 5116 6093 1203
Size: 3913 977 1327
Fragments arranged by size:
3913 1327 977
AgeI A'CCGG_T
Cuts at: 5048 5048
Size: 6217
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6217
AlwNI CAG_nnn'CTG
Cuts at: 3190 3960 5532 3190
Size: 770 1572 3875
Fragments arranged by size:
3875 1572 770
ApaI G_GGCC'C
Cuts at: 2117 2117
Size: 6217
ApaLI G'TGCA_C
Cuts at: 4274 5430 4274
Size: 1156 5061
ApoI r'AATT_y
Cuts at: 1493 3526 3752 3904 6013 1493
Size: 2033 226 152 2109 1697
Fragments arranged by size:
2109 2033 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 2906 3542 1870
Size: 73 963 636 4545
Fragments arranged by size:
4545 963 636 73
BamHI G'GATC_C
Cuts at: 3547 3914 3547
Size: 367 5850
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2041 3311 3538 4993
Size: 325 217 627 224 30 1270 227 1455
Cuts at: 4993 618
Size: 1842
Fragments arranged by size:
1842 1455 1270 627 325 227 224 217
30
BanII G_rGCy'C
Cuts at: 1391 2117 2934 3536 1391
Size: 726 817 602 4072
Fragments arranged by size:
4072 817 726 602
BbeI G_GCGC'C
Cuts at: 2015 2015
Size: 6217
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2204 928
Size: 971 305 4941
Fragments arranged by size:
4941 971 305
BbvCI CC'TCA_GC
Cuts at: 2228 2228
Size: 6217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2099 2133 4408 4442 1903
Size: 34 162 34 2275 34 3678
Fragments arranged by size:
3678 2275 162 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4109 5319 4109
Size: 1210 5007
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6217
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4828 136
Size: 107 122 71 631 605 337 2819 1525
Fragments arranged by size:
2819 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6212 6212
Size: 6217
BlpI GC'TnA_GC
Cuts at: 3634 3634
Size: 6217
Bme1580I G_kGCm'C
Cuts at: 1790 2046 2117 2721 3150 4278 5434 1790
Size: 256 71 604 429 1128 1156 2573
Fragments arranged by size:
2573 1156 1128 604 429 256 71
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6217
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3490 3948 4906
Size: 416 53 83 399 24 2073 458 958
Cuts at: 4906 442
Size: 1753
Fragments arranged by size:
2073 1753 958 458 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6217
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 6217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2317 2441 2558 2635 4877 1239
Size: 1078 124 117 77 2242 2579
Fragments arranged by size:
2579 2242 1078 124 117 77
Bpu10I CC'TnA_GC
Cuts at: 2228 2228
Size: 6217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2342 2918 3481 4342 5207 5505 2342
Size: 576 563 861 865 298 3054
Fragments arranged by size:
3054 865 861 576 563 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4880 882
Size: 617 3381 2219
Fragments arranged by size:
3381 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2716 2770 4008 493
Size: 711 1512 54 1238 2702
Fragments arranged by size:
2702 1512 1238 711 54
BsaBI GATnn'nnATC
Cuts at: 6211 6211
Size: 6217
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 3093 4024 4406
Size: 53 83 186 936 479 1081 931 382
Cuts at: 4406 5041 275
Size: 635 1451
Fragments arranged by size:
1451 1081 936 931 635 479 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4650 5048 5322 5469 5880 4650
Size: 398 274 147 411 4987
Fragments arranged by size:
4987 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5992
Fragments arranged by size:
5992 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2555 1186
Size: 1369 4848
BseYI C'CCAG_C
Cuts at: 2162 2398 2834 5420 5916 2162
Size: 236 436 2586 496 2463
Fragments arranged by size:
2586 2463 496 436 236
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2103 2812 2813 3047 1163
Size: 573 367 709 1 234 4333
Fragments arranged by size:
4333 709 573 367 234 1
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3667 4428 5456 1540
Size: 275 186 1666 761 1028 2301
Fragments arranged by size:
2301 1666 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2934 4278 4363 5434 2934
Size: 1344 85 1071 3717
Fragments arranged by size:
3717 1344 1071 85
BsmI GAATG_Cn'
Cuts at: 3728 3821 3728
Size: 93 6124
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2117 2721 2934 3150 3536 4278
Size: 399 256 71 604 213 216 386 742
Cuts at: 4278 4363 5434 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 604 399 386 256 216
213 85 71
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 6217
BspHI T'CATG_A
Cuts at: 4106 5836 4106
Size: 1730 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2358 2791 3270 3572 844
Size: 1514 433 479 302 3489
Fragments arranged by size:
3489 1514 479 433 302
BsrBI CCG'CTC
Cuts at: 1394 1823 4104 1394
Size: 429 2281 3507
Fragments arranged by size:
3507 2281 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2239 4712 4886 65
Size: 1183 991 2473 174 1396
Fragments arranged by size:
2473 1396 1183 991 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3498 4861 5048 1700
Size: 110 1688 1363 187 2869
Fragments arranged by size:
2869 1688 1363 187 110
BsrGI T'GTAC_A
Cuts at: 96 2487 2991 96
Size: 2391 504 3322
Fragments arranged by size:
3322 2391 504
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6217
BssSI C'ACGA_G
Cuts at: 4277 5289 4277
Size: 1012 5205
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6217
BstBI TT'CG_AA
Cuts at: 5021 5021
Size: 6217
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6217
BstXI CCAn_nnnn'nTGG
Cuts at: 3289 3412 3289
Size: 123 6094
BstYI r'GATC_y
Cuts at: 1972 3395 3547 3914 4299 4316 5768 5854 5866
Size: 1423 152 367 385 17 1452 86 12
Cuts at: 5866 6212 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1423 385 367 346 152 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2365 2811 3487 2365
Size: 446 676 5095
Fragments arranged by size:
5095 676 446
BtgI C'CryG_G
Cuts at: 513 1065 2004 2088 2274 3375 513
Size: 552 939 84 186 1101 3355
Fragments arranged by size:
3355 1101 939 552 186 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2965 487
Size: 683 56 1739 3739
Fragments arranged by size:
3739 1739 683 56
BtsI GCAGTG_nn'
Cuts at: 2726 3733 4527 4547 2726
Size: 1007 794 20 4396
Fragments arranged by size:
4396 1007 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6182
DraI TTT'AAA
Cuts at: 3521 3521
Size: 6217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5224 809
Size: 507 3908 1802
Fragments arranged by size:
3908 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 2445 3117
Size: 54 1425 50 275 18 447 168 672
Cuts at: 3117 3664 4553 6182 8
Size: 547 889 1629 43
Fragments arranged by size:
1629 1425 889 672 547 447 275 168
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3664 1537
Size: 275 1852 4090
Fragments arranged by size:
4090 1852 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3023 4147 1353
Size: 1670 1124 3423
Fragments arranged by size:
3423 1670 1124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4804 5178 5324 387
Size: 1064 138 3215 374 146 1280
Fragments arranged by size:
3215 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2932 2932
Size: 6217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2317 2441 2558 2635 2746 2802 4280
Size: 393 685 124 117 77 111 56 1478
Cuts at: 4280 4877 5664 5932 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1478 787 685 597 393 268 124
117 111 77 56
EcoO109I rG'GnC_Cy
Cuts at: 2113 2114 2418 2938 2113
Size: 1 304 520 5392
Fragments arranged by size:
5392 520 304 1
EcoRI G'AATT_C
Cuts at: 3526 3904 3526
Size: 378 5839
FspI TGC'GCA
Cuts at: 4723 4723
Size: 6217
HaeII r_GCGC'y
Cuts at: 1678 2015 5364 1678
Size: 337 3349 2531
Fragments arranged by size:
3349 2531 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3470 3502 4858 4890 4932
Size: 32 135 32 1898 32 1356 32 42
Cuts at: 4932 4964 1373
Size: 32 2626
Fragments arranged by size:
2626 1898 1356 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2313 3561 3807 669
Size: 988 281 375 1248 246 3079
Fragments arranged by size:
3079 1248 988 375 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6217
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KasI G'GCGC_C
Cuts at: 2011 2011
Size: 6217
KpnI G_GTAC'C
Cuts at: 3542 3542
Size: 6217
MfeI C'AATT_G
Cuts at: 3816 3816
Size: 6217
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2994 3033 5331 5515 1305
Size: 1689 39 2298 184 2007
Fragments arranged by size:
2298 2007 1689 184 39
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2279 2447 10
Size: 54 1425 343 447 168 3780
Fragments arranged by size:
3780 1425 447 343 168 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2087 4175 4534 4693 518
Size: 648 921 2088 359 159 2042
Fragments arranged by size:
2088 2042 921 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2250 2318 2398 2655 2797 3213 3633 4310
Size: 644 68 80 257 142 416 420 677
Cuts at: 4310 5458 5703 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 644 420 416 257 245
142 80 68
NaeI GCC'GGC
Cuts at: 3500 3500
Size: 6217
NarI GG'CG_CC
Cuts at: 2012 2012
Size: 6217
NcoI C'CATG_G
Cuts at: 513 1065 2004 2088 2274 513
Size: 552 939 84 186 4456
Fragments arranged by size:
4456 939 552 186 84
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NgoMIV G'CCGG_C
Cuts at: 3498 3498
Size: 6217
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6217
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 3227 3575 5120 1132
Size: 97 1998 348 1545 2229
Fragments arranged by size:
2229 1998 1545 348 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6217
PciI A'CATG_T
Cuts at: 5116 5116
Size: 6217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6217
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PmlI CAC'GTG
Cuts at: 2716 2770 2716
Size: 54 6163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2418 2938 2418
Size: 520 5697
PshAI GACnn'nnGTC
Cuts at: 1613 2318 3131 1613
Size: 705 813 4699
Fragments arranged by size:
4699 813 705
PsiI TTA'TAA
Cuts at: 3787 3787
Size: 6217
PspOMI G'GGCC_C
Cuts at: 2113 2113
Size: 6217
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2558 2590 2558
Size: 32 6185
PstI C_TGCA'G
Cuts at: 830 2022 2802 3569 830
Size: 1192 780 767 3478
Fragments arranged by size:
3478 1192 780 767
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
PvuII CAG'CTG
Cuts at: 2318 2398 2797 3213 2318
Size: 80 399 416 5322
Fragments arranged by size:
5322 416 399 80
SacI G_AGCT'C
Cuts at: 2934 2934
Size: 6217
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SanDI GG'GwC_CC
Cuts at: 2418 2418
Size: 6217
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 4465 6177 4465
Size: 1712 4505
SexAI A'CCwGG_T
Cuts at: 2577 2577
Size: 6217
SfcI C'TryA_G
Cuts at: 826 1045 2018 2150 2798 3070 3565 4700 5381
Size: 219 973 132 648 272 495 1135 681
Cuts at: 5381 5572 826
Size: 191 1471
Fragments arranged by size:
1471 1135 973 681 648 495 272 219
191 132
SfoI GGC'GCC
Cuts at: 2013 2013
Size: 6217
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6217
SmaI CCC'GGG
Cuts at: 1872 3544 1872
Size: 1672 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2357 2933 3460 4321 5222 5484
Size: 197 926 414 576 527 861 901 262
Cuts at: 5484 820
Size: 1553
Fragments arranged by size:
1553 926 901 861 576 527 414 262
197
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6217
SphI G_CATG'C
Cuts at: 1132 3575 1132
Size: 2443 3774
SspI AAT'ATT
Cuts at: 5 52 4141 5
Size: 47 4089 2081
Fragments arranged by size:
4089 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6217
StyI C'CwwG_G
Cuts at: 513 1065 2004 2088 2274 3656 513
Size: 552 939 84 186 1382 3074
Fragments arranged by size:
3074 1382 939 552 186 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4244 4261 4414 4599 5044 325
Size: 1255 2664 17 153 185 445 1498
Fragments arranged by size:
2664 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2487 2991 3000
Size: 274 80 33 51 1443 510 504 9
Cuts at: 3000 4463 6175 96
Size: 1463 1712 138
Fragments arranged by size:
1712 1463 1443 510 504 274 138 80
51 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6177 6209 6177
Size: 32 6185
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6217
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2878 2878
Size: 6217
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 3542 1870
Size: 1672 4545
XmnI GAAnn'nnTTC
Cuts at: 4346 4346
Size: 6217
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3094 4025 276
Size: 53 83 186 936 1560 931 2468
Fragments arranged by size:
2468 1560 936 931 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PspXI RsrII SacII SapI SfiI
SrfI SwaI