Restriction map for
pFN21AE0313
(Circular) (Six-base) MAPSORT of: pFN21AE0313.seq Check: 5570 from: 1 to: 7714
pFN21AE0313 7714 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3613 3613
Size: 7714
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3447 5524 278
Size: 53 83 186 936 1911 2077 2468
Fragments arranged by size:
2468 2077 1911 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4835 5057 1937
Size: 2898 222 4594
Fragments arranged by size:
4594 2898 222
Acc65I G'GTAC_C
Cuts at: 3766 5035 3766
Size: 1269 6445
AclI AA'CG_TT
Cuts at: 1563 5841 6214 1563
Size: 4278 373 3063
Fragments arranged by size:
4278 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2218 2774 2912 3017 4416 4445 5777 7161
Size: 586 556 138 105 1399 29 1332 1384
Cuts at: 7161 7429 1632
Size: 268 1917
Fragments arranged by size:
1917 1399 1384 1332 586 556 268 138
105 29
AflII C'TTAA_G
Cuts at: 820 1017 4700 820
Size: 197 3683 3834
Fragments arranged by size:
3834 3683 197
AflIII A'CryG_T
Cuts at: 1203 6613 7590 1203
Size: 5410 977 1327
Fragments arranged by size:
5410 1327 977
AgeI A'CCGG_T
Cuts at: 6545 6545
Size: 7714
AhdI GACnn_n'nnGTC
Cuts at: 3085 6443 3085
Size: 3358 4356
AleI CACnn'nnGTG
Cuts at: 1166 3605 1166
Size: 2439 5275
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2468 4281 1184
Size: 1284 1813 4617
Fragments arranged by size:
4617 1813 1284
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2586 2618 2586
Size: 32 7682
AlwNI CAG_nnn'CTG
Cuts at: 3364 3683 5457 7029 3364
Size: 319 1774 1572 4049
Fragments arranged by size:
4049 1774 1572 319
ApaI G_GGCC'C
Cuts at: 2397 2579 4154 4929 2397
Size: 182 1575 775 5182
Fragments arranged by size:
5182 1575 775 182
ApaLI G'TGCA_C
Cuts at: 3254 5771 6927 3254
Size: 2517 1156 4041
Fragments arranged by size:
4041 2517 1156
ApoI r'AATT_y
Cuts at: 1493 4604 4882 5023 5249 5401 7510 1493
Size: 3111 278 141 226 152 2109 1697
Fragments arranged by size:
3111 2109 1697 278 226 152 141
AseI AT'TA_AT
Cuts at: 160 1032 6268 160
Size: 872 5236 1606
Fragments arranged by size:
5236 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7714
AvaI C'yCGr_G
Cuts at: 1870 1943 2220 2406 3438 4734 5039 1870
Size: 73 277 186 1032 1296 305 4545
Fragments arranged by size:
4545 1296 1032 305 277 186 73
BamHI G'GATC_C
Cuts at: 5044 5411 5044
Size: 367 7347
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3091 3433 3542 3584 3766
Size: 325 217 627 1304 342 109 42 182
Cuts at: 3766 5035 6490 618
Size: 1269 1455 1842
Fragments arranged by size:
1842 1455 1304 1269 627 342 325 217
182 109 42
BanII G_rGCy'C
Cuts at: 1391 2397 2579 4154 4475 4929 5013 5033 1391
Size: 1006 182 1575 321 454 84 20 4072
Fragments arranged by size:
4072 1575 1006 454 321 182 84 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2556 928
Size: 971 657 6086
Fragments arranged by size:
6086 971 657
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5905 5939 1903
Size: 34 50 34 3884 34 3678
Fragments arranged by size:
3884 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4094 5606 6816 4094
Size: 1512 1210 4992
Fragments arranged by size:
4992 1512 1210
BclI T'GATC_A
Cuts at: 1548 3913 1548
Size: 2365 5349
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4158 6325 136
Size: 107 122 71 631 605 2486 2167 1525
Fragments arranged by size:
2486 2167 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2331 7709 2331
Size: 5378 2336
BlpI GC'TnA_GC
Cuts at: 3284 4907 5131 3284
Size: 1623 224 5867
Fragments arranged by size:
5867 1623 224
Bme1580I G_kGCm'C
Cuts at: 1790 2397 2579 3096 3258 3385 3545 4154 4929
Size: 607 182 517 162 127 160 609 775
Cuts at: 4929 5775 6931 1790
Size: 846 1156 2573
Fragments arranged by size:
2573 1156 846 775 609 607 517 182
162 160 127
BmgBI CAC'GTC
Cuts at: 1318 2257 1318
Size: 939 6775
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2004 2479 2524
Size: 416 53 83 399 24 587 475 45
Cuts at: 2524 2582 3805 3895 4106 4165 5445 6403 442
Size: 58 1223 90 211 59 1280 958 1753
Fragments arranged by size:
1753 1280 1223 958 587 475 416 399
211 90 83 59 58 53 45 24
BmtI G_CTAG'C
Cuts at: 1056 2327 1056
Size: 1271 6443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2879 3005 3062 3104 3161 3171 3407 3515
Size: 1640 126 57 42 57 10 236 108
Cuts at: 3515 3564 4133 4829 6374 1239
Size: 49 569 696 1545 2579
Fragments arranged by size:
2579 1640 1545 696 569 236 126 108
57 57 49 42 10
Bpu10I CC'TnA_GC
Cuts at: 2844 3305 4469 2844
Size: 461 1164 6089
Fragments arranged by size:
6089 1164 461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3458 5839 6704 7002 3458
Size: 2381 865 298 4170
Fragments arranged by size:
4170 2381 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2824 3898 4035 4209 6377 882
Size: 617 1325 1074 137 174 2168 2219
Fragments arranged by size:
2219 2168 1325 1074 617 174 137
BsaAI yAC'GTr
Cuts at: 493 1204 5505 493
Size: 711 4301 2702
Fragments arranged by size:
4301 2702 711
BsaBI GATnn'nnATC
Cuts at: 7708 7708
Size: 7714
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3444 4488 5521 5903
Size: 53 83 186 936 1911 1044 1033 382
Cuts at: 5903 6538 275
Size: 635 1451
Fragments arranged by size:
1911 1451 1044 1033 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2789 3486 4503 6147 6545 6819 6966 7377 2789
Size: 697 1017 1644 398 274 147 411 3126
Fragments arranged by size:
3126 1644 1017 697 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4982 5012 1178
Size: 30 165 30 3579 30 3880
Fragments arranged by size:
3880 3579 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2158 2290 1186
Size: 972 132 6610
Fragments arranged by size:
6610 972 132
BseYI C'CCAG_C
Cuts at: 2247 2287 2635 2659 2875 2979 3746 4054 4299
Size: 40 348 24 216 104 767 308 245
Cuts at: 4299 6917 7413 2247
Size: 2618 496 2548
Fragments arranged by size:
2618 2548 767 496 348 308 245 216
104 40 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3020 4688 1163
Size: 573 1284 1668 4189
Fragments arranged by size:
4189 1668 1284 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2245 3487 5164 5925 6953 1540
Size: 275 186 244 1242 1677 761 1028 2301
Fragments arranged by size:
2301 1677 1242 1028 761 275 244 186
BsiHKAI G_wGCw'C
Cuts at: 2649 3258 5013 5775 5860 6931 2649
Size: 609 1755 762 85 1071 3432
Fragments arranged by size:
3432 1755 1071 762 609 85
BsmI GAATG_Cn'
Cuts at: 2591 5225 5318 2591
Size: 2634 93 4987
Fragments arranged by size:
4987 2634 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2397 2579 2649 3096 3258 3385 3545
Size: 399 607 182 70 447 162 127 160
Cuts at: 3545 4154 4475 4929 5013 5033 5775 5860 6931
Size: 609 321 454 84 20 742 85 1071
Cuts at: 6931 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 609 607 454 447 399
321 182 162 160 127 85 84 70
20
BspEI T'CCGG_A
Cuts at: 7377 7377
Size: 7714
BspHI T'CATG_A
Cuts at: 5603 7333 5603
Size: 1730 5984
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3613 3876 4875 5069 844
Size: 2769 263 999 194 3489
Fragments arranged by size:
3489 2769 999 263 194
BsrBI CCG'CTC
Cuts at: 1394 1823 2104 4117 4957 5601 1394
Size: 429 281 2013 840 644 3507
Fragments arranged by size:
3507 2013 840 644 429 281
BsrDI GCAATG_nn'
Cuts at: 65 1248 2703 6209 6383 65
Size: 1183 1455 3506 174 1396
Fragments arranged by size:
3506 1455 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2025 2690 3587 3881 6358 6545 1700
Size: 110 215 665 897 294 2477 187 2869
Fragments arranged by size:
2869 2477 897 665 294 215 187 110
BsrGI T'GTAC_A
Cuts at: 96 4140 96
Size: 4044 3670
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7714
BssSI C'ACGA_G
Cuts at: 5774 6786 5774
Size: 1012 6702
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4030 794
Size: 3236 4478
BstBI TT'CG_AA
Cuts at: 6518 6518
Size: 7714
BstEII G'GTnAC_C
Cuts at: 1184 2803 1184
Size: 1619 6095
BstXI CCAn_nnnn'nTGG
Cuts at: 2623 4091 4219 2623
Size: 1468 128 6118
Fragments arranged by size:
6118 1468 128
BstYI r'GATC_y
Cuts at: 1972 2331 4675 5044 5411 5796 5813 7265 7351
Size: 359 2344 369 367 385 17 1452 86
Cuts at: 7351 7363 7709 1972
Size: 12 346 1977
Fragments arranged by size:
2344 1977 1452 385 369 367 359 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 4836 4836
Size: 7714
Bsu36I CC'TnA_GG
Cuts at: 2462 2903 2955 3771 4921 2462
Size: 441 52 816 1150 5255
Fragments arranged by size:
5255 1150 816 441 52
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6975
Fragments arranged by size:
6975 683 56
BtsI GCAGTG_nn'
Cuts at: 2318 2946 3876 3949 4106 5230 6024 6044 2318
Size: 628 930 73 157 1124 794 20 3988
Fragments arranged by size:
3988 1124 930 794 628 157 73 20
ClaI AT'CG_AT
Cuts at: 1554 3936 1554
Size: 2382 5332
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7679
DraI TTT'AAA
Cuts at: 5018 5018
Size: 7714
DraIII CAC_nnn'GTG
Cuts at: 3262 3262
Size: 7714
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6721 809
Size: 507 5405 1802
Fragments arranged by size:
5405 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3946 5161 6050
Size: 54 1425 50 275 18 2116 1215 889
Cuts at: 6050 7679 8
Size: 1629 43
Fragments arranged by size:
2116 1629 1425 1215 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5161 1537
Size: 275 3349 4090
Fragments arranged by size:
4090 3349 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2513 4392 5644 1353
Size: 1160 1879 1252 3423
Fragments arranged by size:
3423 1879 1252 1160
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2417 2493 6301 6675 6821 387
Size: 1064 138 828 76 3808 374 146 1280
Fragments arranged by size:
3808 1280 1064 828 374 146 138 76
EcoICRI GAG'CTC
Cuts at: 5011 5011
Size: 7714
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2218 2774 2879 2912 3005 3017 3062
Size: 393 586 556 105 33 93 12 45
Cuts at: 3062 3104 3161 3171 3407 3515 3564 4133 4416
Size: 42 57 10 236 108 49 569 283
Cuts at: 4416 4445 4829 5777 6374 7161 7429 1239
Size: 29 384 948 597 787 268 1524
Fragments arranged by size:
1524 948 787 597 586 569 556 393
384 283 268 236 108 105 93 57
49 45 42 33 29 12 10
EcoNI CCTnn'n_nnAGG
Cuts at: 4871 4949 4871
Size: 78 7636
EcoO109I rG'GnC_Cy
Cuts at: 2148 2393 2394 2576 2663 3106 3229 4058 4150
Size: 245 1 182 87 443 123 829 92
Cuts at: 4150 4151 4744 4925 4926 4972 2148
Size: 1 593 181 1 46 4890
Fragments arranged by size:
4890 829 593 443 245 182 181 123
92 87 46 1 1 1
EcoRI G'AATT_C
Cuts at: 4604 5023 5401 4604
Size: 419 378 6917
Fragments arranged by size:
6917 419 378
EcoRV GAT'ATC
Cuts at: 3067 3067
Size: 7714
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2158 2635 3304 3358 2158
Size: 477 669 54 6514
Fragments arranged by size:
6514 669 477 54
FspI TGC'GCA
Cuts at: 6220 6220
Size: 7714
HaeII r_GCGC'y
Cuts at: 1678 2876 3403 6861 1678
Size: 1198 527 3458 2531
Fragments arranged by size:
3458 2531 1198 527
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2182 2214 3071 3103 6355
Size: 32 135 32 610 32 857 32 3252
Cuts at: 6355 6387 6429 6461 1373
Size: 32 42 32 2626
Fragments arranged by size:
3252 2626 857 610 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5058 5304 669
Size: 988 281 3120 246 3079
Fragments arranged by size:
3120 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7714
HpaI GTT'AAC
Cuts at: 5304 5304
Size: 7714
KpnI G_GTAC'C
Cuts at: 3770 5039 3770
Size: 1269 6445
MfeI C'AATT_G
Cuts at: 5313 5313
Size: 7714
MluI A'CGCG_T
Cuts at: 7590 7590
Size: 7714
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3696 6828 7012 1305
Size: 2391 3132 184 2007
Fragments arranged by size:
3132 2391 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3948 10
Size: 54 1425 343 2116 3776
Fragments arranged by size:
3776 2116 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3605 3761 4757 4935 5672 6031 6190
Size: 648 2439 156 996 178 737 359 159
Cuts at: 6190 518
Size: 2042
Fragments arranged by size:
2439 2042 996 737 648 359 178 159
156
MspA1I CmG'CkG
Cuts at: 1606 2022 2247 2752 2911 2983 3364 3451 3514
Size: 416 225 505 159 72 381 87 63
Cuts at: 3514 4027 4054 4299 4672 5130 5807 6955 7200
Size: 513 27 245 373 458 677 1148 245
Cuts at: 7200 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 513 505 458 416 381
373 245 245 225 159 87 72 63
27
NaeI GCC'GGC
Cuts at: 2027 2692 3883 2027
Size: 665 1191 5858
Fragments arranged by size:
5858 1191 665
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7162
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7714
NgoMIV G'CCGG_C
Cuts at: 2025 2690 3881 2025
Size: 665 1191 5858
Fragments arranged by size:
5858 1191 665
NheI G'CTAG_C
Cuts at: 1052 2323 1052
Size: 1271 6443
NotI GC'GGCC_GC
Cuts at: 5161 5161
Size: 7714
NruI TCG'CGA
Cuts at: 2100 2100
Size: 7714
NspI r_CATG'y
Cuts at: 1132 1229 5072 6617 1132
Size: 97 3843 1545 2229
Fragments arranged by size:
3843 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7714
PciI A'CATG_T
Cuts at: 6613 6613
Size: 7714
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3102 3655 3955 1108
Size: 1994 553 300 4867
Fragments arranged by size:
4867 1994 553 300
PfoI T'CCnGG_A
Cuts at: 2034 3148 4015 4218 4806 2034
Size: 1114 867 203 588 4942
Fragments arranged by size:
4942 1114 867 588 203
PmeI GTTT'AAAC
Cuts at: 5018 5018
Size: 7714
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5783 5815 7296 7328 5783
Size: 32 1481 32 6169
Fragments arranged by size:
6169 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2148 2663 3106 3229 2148
Size: 515 443 123 6633
Fragments arranged by size:
6633 515 443 123
PshAI GACnn'nnGTC
Cuts at: 1613 2222 3489 1613
Size: 609 1267 5838
Fragments arranged by size:
5838 1267 609
PsiI TTA'TAA
Cuts at: 2121 5284 2121
Size: 3163 4551
PspOMI G'GGCC_C
Cuts at: 2393 2575 4150 4925 2393
Size: 182 1575 775 5182
Fragments arranged by size:
5182 1575 775 182
PstI C_TGCA'G
Cuts at: 830 3054 3519 3846 4260 5066 830
Size: 2224 465 327 414 806 3478
Fragments arranged by size:
3478 2224 806 465 414 327
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7714
PvuII CAG'CTG
Cuts at: 2022 2752 2911 2983 3514 4027 4054 4672 2022
Size: 730 159 72 531 513 27 618 5064
Fragments arranged by size:
5064 730 618 531 513 159 72 27
SacI G_AGCT'C
Cuts at: 5013 5013
Size: 7714
SalI G'TCGA_C
Cuts at: 1936 5056 1936
Size: 3120 4594
SanDI GG'GwC_CC
Cuts at: 2148 3106 2148
Size: 958 6756
SbfI CC_TGCA'GG
Cuts at: 5066 5066
Size: 7714
ScaI AGT'ACT
Cuts at: 5962 7674 5962
Size: 1712 6002
SfcI C'TryA_G
Cuts at: 826 1045 3050 3515 3842 3978 4198 4256 5062
Size: 219 2005 465 327 136 220 58 806
Cuts at: 5062 6197 6878 7069 826
Size: 1135 681 191 1471
Fragments arranged by size:
2005 1471 1135 806 681 465 327 220
219 191 136 58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7714
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7714
SmaI CCC'GGG
Cuts at: 1872 4736 5041 1872
Size: 2864 305 4545
Fragments arranged by size:
4545 2864 305
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3473 4700 5818 6719 6981 820
Size: 197 926 1530 1227 1118 901 262 1553
Fragments arranged by size:
1553 1530 1227 1118 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5505 493
Size: 5012 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7714
SphI G_CATG'C
Cuts at: 1132 5072 1132
Size: 3940 3774
SspI AAT'ATT
Cuts at: 5 52 5638 5
Size: 47 5586 2081
Fragments arranged by size:
5586 2081 47
StuI AGG'CCT
Cuts at: 1517 4262 1517
Size: 2745 4969
StyI C'CwwG_G
Cuts at: 513 1065 4609 4740 5153 513
Size: 552 3544 131 413 3074
Fragments arranged by size:
3544 3074 552 413 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5741 5758 5911 6096 6541 325
Size: 1255 4161 17 153 185 445 1498
Fragments arranged by size:
4161 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4140 5960 7672
Size: 274 80 33 51 1443 2163 1820 1712
Cuts at: 7672 96
Size: 138
Fragments arranged by size:
2163 1820 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2356 2388 2784 2816 3535 3567 4982 5014 7674
Size: 32 396 32 719 32 1415 32 2660
Cuts at: 7674 7706 2356
Size: 32 2364
Fragments arranged by size:
2660 2364 1415 719 396 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3275 1316
Size: 1959 5755
XbaI T'CTAG_A
Cuts at: 5050 5050
Size: 7714
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7714
XmaI C'CCGG_G
Cuts at: 1870 4734 5039 1870
Size: 2864 305 4545
Fragments arranged by size:
4545 2864 305
XmnI GAAnn'nnTTC
Cuts at: 4802 5843 4802
Size: 1041 6673
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3445 5522 276
Size: 53 83 186 936 1911 2077 2468
Fragments arranged by size:
2468 2077 1911 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI FseI FspAI KasI NarI NsiI PacI
PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI
SfoI SrfI SwaI XcmI