Restriction map for
pFN21AE0311
(Circular) (Six-base) MAPSORT of: pFN21AE0311.seq Check: 3033 from: 1 to: 8500
pFN21AE0311 8500 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4312 4312
Size: 8500
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3696 5234 6310 278
Size: 53 83 186 936 2160 1538 1076 2468
Fragments arranged by size:
2468 2160 1538 1076 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5324 5843 1937
Size: 3387 519 4594
Fragments arranged by size:
4594 3387 519
Acc65I G'GTAC_C
Cuts at: 5821 5821
Size: 8500
AclI AA'CG_TT
Cuts at: 1563 6627 7000 1563
Size: 5064 373 3063
Fragments arranged by size:
5064 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2559 4197 5706 6563 7947 8215 1632
Size: 927 1638 1509 857 1384 268 1917
Fragments arranged by size:
1917 1638 1509 1384 927 857 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8303
AflIII A'CryG_T
Cuts at: 1203 3888 4798 7399 8376 1203
Size: 2685 910 2601 977 1327
Fragments arranged by size:
2685 2601 1327 977 910
AgeI A'CCGG_T
Cuts at: 7331 7331
Size: 8500
AhdI GACnn_n'nnGTC
Cuts at: 7229 7229
Size: 8500
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8500
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2474 1184
Size: 1290 7210
AlwNI CAG_nnn'CTG
Cuts at: 5099 6243 7815 5099
Size: 1144 1572 5784
Fragments arranged by size:
5784 1572 1144
ApaLI G'TGCA_C
Cuts at: 6557 7713 6557
Size: 1156 7344
ApoI r'AATT_y
Cuts at: 1493 2076 2215 2272 3539 3557 3574 3704 3742
Size: 583 139 57 1267 18 17 130 38
Cuts at: 3742 4586 4624 4929 5527 5597 5809 6035 6187
Size: 844 38 305 598 70 212 226 152
Cuts at: 6187 8296 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1267 844 598 583 305 226
212 152 139 130 70 57 38 38
18 17
AseI AT'TA_AT
Cuts at: 160 1032 3831 7054 160
Size: 872 2799 3223 1606
Fragments arranged by size:
3223 2799 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8500
AvaI C'yCGr_G
Cuts at: 1870 1943 5825 1870
Size: 73 3882 4545
Fragments arranged by size:
4545 3882 73
BamHI G'GATC_C
Cuts at: 5830 6197 5830
Size: 367 8133
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5821 7276 618
Size: 325 217 627 4034 1455 1842
Fragments arranged by size:
4034 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 5819 1391
Size: 4428 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2456 3866 4166 4287 4748 5258 928
Size: 971 557 1410 300 121 461 510 4170
Fragments arranged by size:
4170 1410 971 557 510 461 300 121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3112 3146 6691 6725 1903
Size: 34 1175 34 3545 34 3678
Fragments arranged by size:
3678 3545 1175 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6392 7602 6392
Size: 1210 7290
BclI T'GATC_A
Cuts at: 1548 4924 1548
Size: 3376 5124
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7111 136
Size: 107 122 71 631 605 5439 1525
Fragments arranged by size:
5439 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3308 4822 5167 5414 5713 8495 3308
Size: 1514 345 247 299 2782 3313
Fragments arranged by size:
3313 2782 1514 345 299 247
BlpI GC'TnA_GC
Cuts at: 4692 5917 4692
Size: 1225 7275
Bme1580I G_kGCm'C
Cuts at: 1790 2015 6561 7717 1790
Size: 225 4546 1156 2573
Fragments arranged by size:
4546 2573 1156 225
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8500
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2009 2764 5108
Size: 416 53 83 399 24 592 755 2344
Cuts at: 5108 6231 7189 442
Size: 1123 958 1753
Fragments arranged by size:
2344 1753 1123 958 755 592 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2424 1056
Size: 1368 7132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5256 7160 1239
Size: 4017 1904 2579
Fragments arranged by size:
4017 2579 1904
Bpu10I CC'TnA_GC
Cuts at: 2190 3399 4139 4721 2190
Size: 1209 740 582 5969
Fragments arranged by size:
5969 1209 740 582
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6625 7490 7788 6625
Size: 865 298 7337
Fragments arranged by size:
7337 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7163 882
Size: 617 5664 2219
Fragments arranged by size:
5664 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6291 493
Size: 711 5087 2702
Fragments arranged by size:
5087 2702 711
BsaBI GATnn'nnATC
Cuts at: 5100 8494 5100
Size: 3394 5106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3693 5231 6307 6689
Size: 53 83 186 936 2160 1538 1076 382
Cuts at: 6689 7324 275
Size: 635 1451
Fragments arranged by size:
2160 1538 1451 1076 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3855 5610 6933 7331 7605 7752 8163 3855
Size: 1755 1323 398 274 147 411 4192
Fragments arranged by size:
4192 1755 1323 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8275
Fragments arranged by size:
8275 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 8500
BseYI C'CCAG_C
Cuts at: 4012 5548 7703 8199 4012
Size: 1536 2155 496 4313
Fragments arranged by size:
4313 2155 1536 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3357 1163
Size: 573 1621 6306
Fragments arranged by size:
6306 1621 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5950 6711 7739 1540
Size: 275 186 3949 761 1028 2301
Fragments arranged by size:
3949 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5546 6561 6646 7717 5546
Size: 1015 85 1071 6329
Fragments arranged by size:
6329 1071 1015 85
BsmI GAATG_Cn'
Cuts at: 4868 6011 6104 4868
Size: 1143 93 7264
Fragments arranged by size:
7264 1143 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 5546 5819 6561 6646 7717 1391
Size: 399 225 3531 273 742 85 1071 2174
Fragments arranged by size:
3531 2174 1071 742 399 273 225 85
BspEI T'CCGG_A
Cuts at: 8163 8163
Size: 8500
BspHI T'CATG_A
Cuts at: 2343 6389 8119 2343
Size: 4046 1730 2724
Fragments arranged by size:
4046 2724 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4312 4399 5855 844
Size: 3468 87 1456 3489
Fragments arranged by size:
3489 3468 1456 87
BsrBI CCG'CTC
Cuts at: 1394 1823 6387 1394
Size: 429 4564 3507
Fragments arranged by size:
4564 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6995 7169 65
Size: 1183 5747 174 1396
Fragments arranged by size:
5747 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7144 7331 1700
Size: 110 5334 187 2869
Fragments arranged by size:
5334 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4501 96
Size: 4405 4095
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8500
BssSI C'ACGA_G
Cuts at: 6560 7572 6560
Size: 1012 7488
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8500
BstBI TT'CG_AA
Cuts at: 7304 7304
Size: 8500
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8500
BstXI CCAn_nnnn'nTGG
Cuts at: 2777 2777
Size: 8500
BstYI r'GATC_y
Cuts at: 1972 2986 3308 3343 4822 5167 5263 5414 5713
Size: 1014 322 35 1479 345 96 151 299
Cuts at: 5713 5830 6197 6582 6599 8051 8137 8149 8495
Size: 117 367 385 17 1452 86 12 346
Cuts at: 8495 1972
Size: 1977
Fragments arranged by size:
1977 1479 1452 1014 385 367 346 345
322 299 151 117 96 86 35 17
12
Bsu36I CC'TnA_GG
Cuts at: 2044 2044
Size: 8500
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7009
Fragments arranged by size:
7009 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7761
Fragments arranged by size:
7761 683 56
BtsI GCAGTG_nn'
Cuts at: 2534 5685 6016 6810 6830 2534
Size: 3151 331 794 20 4204
Fragments arranged by size:
4204 3151 794 331 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8500
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4306 4341 529
Size: 35 3742 35 4688
Fragments arranged by size:
4688 3742 35 35
DraI TTT'AAA
Cuts at: 2166 3256 4834 5073 5602 5767 5804 2166
Size: 1090 1578 239 529 165 37 4862
Fragments arranged by size:
4862 1578 1090 529 239 165 37
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5236 7507 809
Size: 507 3920 2271 1802
Fragments arranged by size:
3920 2271 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2779 4375 5947
Size: 54 1425 50 275 18 949 1596 1572
Cuts at: 5947 6836 8465 8
Size: 889 1629 43
Fragments arranged by size:
1629 1596 1572 1425 949 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5947 1537
Size: 275 4135 4090
Fragments arranged by size:
4135 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2578 5791 6430 1353
Size: 1225 3213 639 3423
Fragments arranged by size:
3423 3213 1225 639
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7087 7461 7607 387
Size: 1064 138 5498 374 146 1280
Fragments arranged by size:
5498 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2559 4197 5256 5706 6563 7160 7947
Size: 393 927 1638 1059 450 857 597 787
Cuts at: 7947 8215 1239
Size: 268 1524
Fragments arranged by size:
1638 1524 1059 927 857 787 597 450
393 268
EcoO109I rG'GnC_Cy
Cuts at: 3759 4056 3759
Size: 297 8203
EcoRI G'AATT_C
Cuts at: 2215 3574 5809 6187 2215
Size: 1359 2235 378 4528
Fragments arranged by size:
4528 2235 1359 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3618 5410 3618
Size: 1792 6708
FspI TGC'GCA
Cuts at: 7006 7006
Size: 8500
HaeII r_GCGC'y
Cuts at: 1678 7647 1678
Size: 5969 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2555 2587 2783 2815 2926
Size: 32 135 32 983 32 196 32 111
Cuts at: 2926 2958 3203 3235 3300 3332 4670 4702 7141
Size: 32 245 32 65 32 1338 32 2439
Cuts at: 7141 7173 7215 7247 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2439 1338 983 245 196 135 111
65 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4900 5031 5608 5844 6090 669
Size: 988 281 2962 131 577 236 246 3079
Fragments arranged by size:
3079 2962 988 577 281 246 236 131
HindIII A'AGCT_T
Cuts at: 748 2321 3600 3836 4136 748
Size: 1573 1279 236 300 5112
Fragments arranged by size:
5112 1573 1279 300 236
HpaI GTT'AAC
Cuts at: 5031 6090 5031
Size: 1059 7441
KpnI G_GTAC'C
Cuts at: 5825 5825
Size: 8500
MfeI C'AATT_G
Cuts at: 3068 6099 3068
Size: 3031 5469
MluI A'CGCG_T
Cuts at: 8376 8376
Size: 8500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7614 7798 1305
Size: 6309 184 2007
Fragments arranged by size:
6309 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4377 10
Size: 54 1425 343 2545 4133
Fragments arranged by size:
4133 2545 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2949 3891 4094 4377 6458 6817 6976
Size: 648 1783 942 203 283 2081 359 159
Cuts at: 6976 518
Size: 2042
Fragments arranged by size:
2081 2042 1783 942 648 359 283 203
159
MspA1I CmG'CkG
Cuts at: 1606 3373 3394 5916 6593 7741 7986 1606
Size: 1767 21 2522 677 1148 245 2120
Fragments arranged by size:
2522 2120 1767 1148 677 245 21
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7009
Fragments arranged by size:
7009 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8500
NheI G'CTAG_C
Cuts at: 1052 2420 1052
Size: 1368 7132
NotI GC'GGCC_GC
Cuts at: 5947 5947
Size: 8500
NspI r_CATG'y
Cuts at: 1132 1229 3892 4802 5858 7403 1132
Size: 97 2663 910 1056 1545 2229
Fragments arranged by size:
2663 2229 1545 1056 910 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8500
PciI A'CATG_T
Cuts at: 3888 4798 7399 3888
Size: 910 2601 4989
Fragments arranged by size:
4989 2601 910
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8500
PfoI T'CCnGG_A
Cuts at: 3318 5104 3318
Size: 1786 6714
PmeI GTTT'AAAC
Cuts at: 5804 5804
Size: 8500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6569 6601 8082 8114 6569
Size: 32 1481 32 6955
Fragments arranged by size:
6955 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3759 4056 3759
Size: 297 8203
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8500
PsiI TTA'TAA
Cuts at: 6070 6070
Size: 8500
PstI C_TGCA'G
Cuts at: 830 2866 5852 830
Size: 2036 2986 3478
Fragments arranged by size:
3478 2986 2036
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8500
PvuII CAG'CTG
Cuts at: 3373 3394 3373
Size: 21 8479
SalI G'TCGA_C
Cuts at: 1936 5842 1936
Size: 3906 4594
SanDI GG'GwC_CC
Cuts at: 3759 3759
Size: 8500
SbfI CC_TGCA'GG
Cuts at: 5852 5852
Size: 8500
ScaI AGT'ACT
Cuts at: 6748 8460 6748
Size: 1712 6788
SfcI C'TryA_G
Cuts at: 826 1045 2685 2862 4051 5848 6983 7664 7855
Size: 219 1640 177 1189 1797 1135 681 191
Cuts at: 7855 826
Size: 1471
Fragments arranged by size:
1797 1640 1471 1189 1135 681 219 191
177
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8500
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8500
SmaI CCC'GGG
Cuts at: 1872 5827 1872
Size: 3955 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6604 7505 7767 820
Size: 197 926 4661 901 262 1553
Fragments arranged by size:
4661 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6291 493
Size: 5798 2702
SpeI A'CTAG_T
Cuts at: 152 5084 152
Size: 4932 3568
SphI G_CATG'C
Cuts at: 1132 5858 1132
Size: 4726 3774
SspI AAT'ATT
Cuts at: 5 52 2542 6424 5
Size: 47 2490 3882 2081
Fragments arranged by size:
3882 2490 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8500
StyI C'CwwG_G
Cuts at: 513 1065 2004 5535 5939 513
Size: 552 939 3531 404 3074
Fragments arranged by size:
3531 3074 939 552 404
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4658 6527 6544 6697 6882 7327 325
Size: 1255 3078 1869 17 153 185 445 1498
Fragments arranged by size:
3078 1869 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3463 4501 5750
Size: 274 80 33 51 1443 1486 1038 1249
Cuts at: 5750 6746 8458 96
Size: 996 1712 138
Fragments arranged by size:
1712 1486 1443 1249 1038 996 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2815 2847 8460 8492 2815
Size: 32 5613 32 2823
Fragments arranged by size:
5613 2823 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8500
XbaI T'CTAG_A
Cuts at: 3605 5836 3605
Size: 2231 6269
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8500
XmaI C'CCGG_G
Cuts at: 1870 5825 1870
Size: 3955 4545
XmnI GAAnn'nnTTC
Cuts at: 3194 4458 6629 3194
Size: 1264 2171 5065
Fragments arranged by size:
5065 2171 1264
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3694 5232 6308 276
Size: 53 83 186 936 2160 1538 1076 2468
Fragments arranged by size:
2468 2160 1538 1076 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI