Restriction map for
pFN21AE0305
(Circular) (Six-base) MAPSORT of: pFN21AE0305.seq Check: 2322 from: 1 to: 6661
pFN21AE0305 6661 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3680 3680
Size: 6661
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4471 278
Size: 53 83 186 936 2935 2468
Fragments arranged by size:
2935 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3556 4004 1937
Size: 1619 448 4594
Fragments arranged by size:
4594 1619 448
Acc65I G'GTAC_C
Cuts at: 3003 3982 3003
Size: 979 5682
AclI AA'CG_TT
Cuts at: 1563 4788 5161 1563
Size: 3225 373 3063
Fragments arranged by size:
3225 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2329 2357 4724 6108 6376 1632
Size: 697 28 2367 1384 268 1917
Fragments arranged by size:
2367 1917 1384 697 268 28
AfeI AGC'GCT
Cuts at: 2140 2423 2140
Size: 283 6378
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6464
AflIII A'CryG_T
Cuts at: 1203 2982 5560 6537 1203
Size: 1779 2578 977 1327
Fragments arranged by size:
2578 1779 1327 977
AgeI A'CCGG_T
Cuts at: 5492 5492
Size: 6661
AhdI GACnn_n'nnGTC
Cuts at: 2706 5390 2706
Size: 2684 3977
AleI CACnn'nnGTG
Cuts at: 1166 3773 1166
Size: 2607 4054
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6661
AlwNI CAG_nnn'CTG
Cuts at: 2242 4404 5976 2242
Size: 2162 1572 2927
Fragments arranged by size:
2927 2162 1572
ApaI G_GGCC'C
Cuts at: 2495 2591 2768 3305 3314 3444 3521 2495
Size: 96 177 537 9 130 77 5635
Fragments arranged by size:
5635 537 177 130 96 77 9
ApaLI G'TGCA_C
Cuts at: 4718 5874 4718
Size: 1156 5505
ApoI r'AATT_y
Cuts at: 1493 3970 4196 4348 6457 1493
Size: 2477 226 152 2109 1697
Fragments arranged by size:
2477 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5215 160
Size: 872 4183 1606
Fragments arranged by size:
4183 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6661
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2125 2761 3016 3304 3521 3986
Size: 73 114 68 636 255 288 217 465
Cuts at: 3986 1870
Size: 4545
Fragments arranged by size:
4545 636 465 288 255 217 114 73
68
AvrII C'CTAG_G
Cuts at: 3763 3763
Size: 6661
BamHI G'GATC_C
Cuts at: 3991 4358 3991
Size: 367 6294
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3003 3527 3767 3982 5437
Size: 325 217 627 1216 524 240 215 1455
Cuts at: 5437 618
Size: 1842
Fragments arranged by size:
1842 1455 1216 627 524 325 240 217
215
BanII G_rGCy'C
Cuts at: 1391 2295 2400 2495 2507 2591 2768 3305 3314
Size: 904 105 95 12 84 177 537 9
Cuts at: 3314 3444 3521 3645 3980 1391
Size: 130 77 124 335 4072
Fragments arranged by size:
4072 904 537 335 177 130 124 105
95 84 77 12 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5690
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2901 2935 4852 4886 1903
Size: 34 964 34 1917 34 3678
Fragments arranged by size:
3678 1917 964 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4553 5763 4553
Size: 1210 5451
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6661
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2264 2663 3309
Size: 107 122 71 631 605 592 399 646
Cuts at: 3309 3372 3525 5272 136
Size: 63 153 1747 1525
Fragments arranged by size:
1747 1525 646 631 605 592 399 153
122 107 71 63
BglII A'GATC_T
Cuts at: 6656 6656
Size: 6661
BlpI GC'TnA_GC
Cuts at: 2135 4078 2135
Size: 1943 4718
Bme1580I G_kGCm'C
Cuts at: 1790 2495 2591 2652 2768 3070 3305 3314 3444
Size: 705 96 61 116 302 235 9 130
Cuts at: 3444 3489 3521 3530 4722 5878 1790
Size: 45 32 9 1192 1156 2573
Fragments arranged by size:
2573 1192 1156 705 302 235 130 116
96 61 45 32 9 9
BmgBI CAC'GTC
Cuts at: 1318 2218 3040 3286 1318
Size: 900 822 246 4693
Fragments arranged by size:
4693 900 822 246
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2761 3609 4392
Size: 416 53 83 399 24 1344 848 783
Cuts at: 4392 5350 442
Size: 958 1753
Fragments arranged by size:
1753 1344 958 848 783 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2279 2911 3159 3655 5321 1239
Size: 1040 632 248 496 1666 2579
Fragments arranged by size:
2579 1666 1040 632 496 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3518 3584 4786 5651 5949 3518
Size: 66 1202 865 298 4230
Fragments arranged by size:
4230 1202 865 298 66
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3403 5324 882
Size: 617 1904 1921 2219
Fragments arranged by size:
2219 1921 1904 617
BsaAI yAC'GTr
Cuts at: 493 1204 2952 2985 4452 493
Size: 711 1748 33 1467 2702
Fragments arranged by size:
2702 1748 1467 711 33
BsaBI GATnn'nnATC
Cuts at: 6655 6655
Size: 6661
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3576 3630 4468 4850
Size: 53 83 186 936 2043 54 838 382
Cuts at: 4850 5485 275
Size: 635 1451
Fragments arranged by size:
2043 1451 936 838 635 382 186 83
54 53
BsaWI w'CCGG_w
Cuts at: 2400 5094 5492 5766 5913 6324 2400
Size: 2694 398 274 147 411 2737
Fragments arranged by size:
2737 2694 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3872 3902 3917 3947 1178
Size: 30 165 30 2469 30 15 30 3892
Fragments arranged by size:
3892 2469 165 30 30 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2219 3254 3467 3691 3860 1186
Size: 1033 1035 213 224 169 3987
Fragments arranged by size:
3987 1035 1033 224 213 169
BseYI C'CCAG_C
Cuts at: 2052 2211 2235 2349 2451 2465 3111 3375 3644
Size: 159 24 114 102 14 646 264 269
Cuts at: 3644 5864 6360 2052
Size: 2220 496 2353
Fragments arranged by size:
2353 2220 646 496 269 264 159 114
102 24 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3187 1163
Size: 573 1451 4637
Fragments arranged by size:
4637 1451 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4111 4872 5900 1540
Size: 275 186 2110 761 1028 2301
Fragments arranged by size:
2301 2110 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2400 3175 3482 4722 4807 5878 2400
Size: 775 307 1240 85 1071 3183
Fragments arranged by size:
3183 1240 1071 775 307 85
BsmI GAATG_Cn'
Cuts at: 3053 3113 3877 4172 4265 3053
Size: 60 764 295 93 5449
Fragments arranged by size:
5449 764 295 93 60
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2295 2400 2495 2507 2591 2652 2768
Size: 399 505 105 95 12 84 61 116
Cuts at: 2768 3070 3175 3305 3314 3444 3482 3489 3521
Size: 302 105 130 9 130 38 7 32
Cuts at: 3521 3530 3645 3980 4722 4807 5878 1391
Size: 9 115 335 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 505 399 335 302 130
130 116 115 105 105 95 85 84
61 38 32 12 9 9 7
BspEI T'CCGG_A
Cuts at: 2400 6324 2400
Size: 3924 2737
BspHI T'CATG_A
Cuts at: 4550 6280 4550
Size: 1730 4931
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3680 4016 844
Size: 2836 336 3489
Fragments arranged by size:
3489 2836 336
BsrBI CCG'CTC
Cuts at: 1394 1823 2458 4548 1394
Size: 429 635 2090 3507
Fragments arranged by size:
3507 2090 635 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5156 5330 65
Size: 1183 3908 174 1396
Fragments arranged by size:
3908 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2568 5305 5492 1700
Size: 110 758 2737 187 2869
Fragments arranged by size:
2869 2737 758 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6661
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6661
BssSI C'ACGA_G
Cuts at: 2407 4721 5733 2407
Size: 2314 1012 3335
Fragments arranged by size:
3335 2314 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3745 794
Size: 2951 3710
BstBI TT'CG_AA
Cuts at: 5465 5465
Size: 6661
BstEII G'GTnAC_C
Cuts at: 1184 2599 2896 1184
Size: 1415 297 4949
Fragments arranged by size:
4949 1415 297
BstXI CCAn_nnnn'nTGG
Cuts at: 2017 3633 2017
Size: 1616 5045
BstYI r'GATC_y
Cuts at: 1972 3991 4358 4743 4760 6212 6298 6310 6656
Size: 2019 367 385 17 1452 86 12 346
Cuts at: 6656 1972
Size: 1977
Fragments arranged by size:
2019 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2243 2243
Size: 6661
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2968 3408 3705 3771 3921
Size: 552 939 12 952 440 297 66 150
Cuts at: 3921 513
Size: 3253
Fragments arranged by size:
3253 952 939 552 440 297 150 66
12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5922
Fragments arranged by size:
5922 683 56
BtsI GCAGTG_nn'
Cuts at: 2158 3226 4177 4971 4991 2158
Size: 1068 951 794 20 3828
Fragments arranged by size:
3828 1068 951 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6661
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6626
DraI TTT'AAA
Cuts at: 3965 3965
Size: 6661
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5668 809
Size: 507 4352 1802
Fragments arranged by size:
4352 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2184 2664
Size: 54 1425 50 275 18 177 177 480
Cuts at: 2664 3255 3708 3777 4108 4997 6626 8
Size: 591 453 69 331 889 1629 43
Fragments arranged by size:
1629 1425 889 591 480 453 331 275
177 177 69 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4108 1537
Size: 275 2296 4090
Fragments arranged by size:
4090 2296 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2189 3833 4591 1353
Size: 836 1644 758 3423
Fragments arranged by size:
3423 1644 836 758
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5248 5622 5768 387
Size: 1064 138 3659 374 146 1280
Fragments arranged by size:
3659 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2398 2398
Size: 6661
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2279 2329 2357 2911 3159 3655 4724
Size: 393 647 50 28 554 248 496 1069
Cuts at: 4724 5321 6108 6376 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1069 787 647 597 554 496 393
268 248 50 28
EcoNI CCTnn'n_nnAGG
Cuts at: 2723 2723
Size: 6661
EcoO109I rG'GnC_Cy
Cuts at: 2491 2492 2587 2734 2765 2963 3147 3310 3311
Size: 1 95 147 31 198 184 163 1
Cuts at: 3311 3348 3413 3441 3517 3518 3621 2491
Size: 37 65 28 76 1 103 5531
Fragments arranged by size:
5531 198 184 163 147 103 95 76
65 37 31 28 1 1 1
EcoRI G'AATT_C
Cuts at: 3970 4348 3970
Size: 378 6283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2569 3576 2569
Size: 1007 5654
FspI TGC'GCA
Cuts at: 5167 5167
Size: 6661
HaeII r_GCGC'y
Cuts at: 1678 2142 2425 5808 1678
Size: 464 283 3383 2531
Fragments arranged by size:
3383 2531 464 283
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2692 2724 3872 3904 5302
Size: 32 135 32 1120 32 1148 32 1398
Cuts at: 5302 5334 5376 5408 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1398 1148 1120 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3082 4005 4251 669
Size: 988 281 1144 923 246 3079
Fragments arranged by size:
3079 1144 988 923 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6661
HpaI GTT'AAC
Cuts at: 4251 4251
Size: 6661
KpnI G_GTAC'C
Cuts at: 3007 3986 3007
Size: 979 5682
MfeI C'AATT_G
Cuts at: 4260 4260
Size: 6661
MluI A'CGCG_T
Cuts at: 6537 6537
Size: 6661
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3965 5775 5959 1305
Size: 2660 1810 184 2007
Fragments arranged by size:
2660 2007 1810 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2186 3710 3779 10
Size: 54 1425 343 177 177 1524 69 2892
Fragments arranged by size:
2892 1524 1425 343 177 177 69 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2015 3773 4619 4978 5137 518
Size: 648 849 1758 846 359 159 2042
Fragments arranged by size:
2042 1758 849 846 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2335 2349 2991 3010 3025 3102 3665 4077
Size: 729 14 642 19 15 77 563 412
Cuts at: 4077 4754 5902 6147 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 729 677 642 563 412 245
77 19 15 14
NaeI GCC'GGC
Cuts at: 2570 2570
Size: 6661
NcoI C'CATG_G
Cuts at: 513 1065 2004 2016 2968 3408 3921 513
Size: 552 939 12 952 440 513 3253
Fragments arranged by size:
3253 952 939 552 513 440 12
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6661
NgoMIV G'CCGG_C
Cuts at: 2568 2568
Size: 6661
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6661
NotI GC'GGCC_GC
Cuts at: 4108 4108
Size: 6661
NspI r_CATG'y
Cuts at: 1132 1229 2566 4019 5564 1132
Size: 97 1337 1453 1545 2229
Fragments arranged by size:
2229 1545 1453 1337 97
PasI CC'CwG_GG
Cuts at: 1348 2581 3089 3196 1348
Size: 1233 508 107 4813
Fragments arranged by size:
4813 1233 508 107
PciI A'CATG_T
Cuts at: 5560 5560
Size: 6661
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2939 3286 3437 1108
Size: 1831 347 151 4332
Fragments arranged by size:
4332 1831 347 151
PfoI T'CCnGG_A
Cuts at: 3136 3136
Size: 6661
PmeI GTTT'AAAC
Cuts at: 3965 3965
Size: 6661
PmlI CAC'GTG
Cuts at: 2952 2985 2952
Size: 33 6628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4730 4762 6243 6275 4730
Size: 32 1481 32 5116
Fragments arranged by size:
5116 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2963 3348 3621 2963
Size: 385 273 6003
Fragments arranged by size:
6003 385 273
PshAI GACnn'nnGTC
Cuts at: 1613 2325 3018 1613
Size: 712 693 5256
Fragments arranged by size:
5256 712 693
PsiI TTA'TAA
Cuts at: 4231 4231
Size: 6661
PspOMI G'GGCC_C
Cuts at: 2491 2587 2764 3301 3310 3440 3517 2491
Size: 96 177 537 9 130 77 5635
Fragments arranged by size:
5635 537 177 130 96 77 9
PspXI vC'TCGA_Gb
Cuts at: 3016 3016
Size: 6661
PstI C_TGCA'G
Cuts at: 830 3691 4013 830
Size: 2861 322 3478
Fragments arranged by size:
3478 2861 322
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6661
PvuII CAG'CTG
Cuts at: 2349 3010 3025 3665 2349
Size: 661 15 640 5345
Fragments arranged by size:
5345 661 640 15
SacI G_AGCT'C
Cuts at: 2400 2400
Size: 6661
SalI G'TCGA_C
Cuts at: 1936 4003 1936
Size: 2067 4594
SanDI GG'GwC_CC
Cuts at: 2963 3621 2963
Size: 658 6003
SapI GCTCTTCn'nnn_
Cuts at: 2189 2189
Size: 6661
SbfI CC_TGCA'GG
Cuts at: 3691 4013 3691
Size: 322 6339
ScaI AGT'ACT
Cuts at: 2229 4909 6621 2229
Size: 2680 1712 2269
Fragments arranged by size:
2680 2269 1712
SexAI A'CCwGG_T
Cuts at: 2892 2892
Size: 6661
SfcI C'TryA_G
Cuts at: 826 1045 3687 4009 5144 5825 6016 826
Size: 219 2642 322 1135 681 191 1471
Fragments arranged by size:
2642 1471 1135 681 322 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3309 3309
Size: 6661
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6661
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6661
SmaI CCC'GGG
Cuts at: 1872 2059 3306 3988 1872
Size: 187 1247 682 4545
Fragments arranged by size:
4545 1247 682 187
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3016 3533 3599 4765 5666 5928
Size: 197 926 1073 517 66 1166 901 262
Cuts at: 5928 820
Size: 1553
Fragments arranged by size:
1553 1166 1073 926 901 517 262 197
66
SnaBI TAC'GTA
Cuts at: 493 4452 493
Size: 3959 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6661
SphI G_CATG'C
Cuts at: 1132 4019 1132
Size: 2887 3774
SspI AAT'ATT
Cuts at: 5 52 4585 5
Size: 47 4533 2081
Fragments arranged by size:
4533 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6661
StyI C'CwwG_G
Cuts at: 513 1065 2004 2016 2637 2968 2998 3408 3763
Size: 552 939 12 621 331 30 410 355
Cuts at: 3763 3921 4100 513
Size: 158 179 3074
Fragments arranged by size:
3074 939 621 552 410 355 331 179
158 30 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2341 3367 3559 4688 4705 4858 5043
Size: 1255 761 1026 192 1129 17 153 185
Cuts at: 5043 5488 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1129 1026 761 445 192 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2227 3933 4907
Size: 274 80 33 51 1443 250 1706 974
Cuts at: 4907 6619 96
Size: 1712 138
Fragments arranged by size:
1712 1706 1443 974 274 250 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6621 6653 6621
Size: 32 6629
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6661
XbaI T'CTAG_A
Cuts at: 3997 3997
Size: 6661
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2635 2956 2635
Size: 321 6340
XhoI C'TCGA_G
Cuts at: 1943 3016 1943
Size: 1073 5588
XmaI C'CCGG_G
Cuts at: 1870 2057 3304 3986 1870
Size: 187 1247 682 4545
Fragments arranged by size:
4545 1247 682 187
XmnI GAAnn'nnTTC
Cuts at: 2342 4790 2342
Size: 2448 4213
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4469 276
Size: 53 83 186 936 2935 2468
Fragments arranged by size:
2935 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PsrI RsrII SacII SfoI
SrfI SwaI