Restriction map for
pFN21AE0304
(Circular) (Six-base) MAPSORT of: pFN21AE0304.seq Check: 6898 from: 1 to: 6172
pFN21AE0304 6172 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3982 278
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2457 3515 1937
Size: 520 1058 4594
Fragments arranged by size:
4594 1058 520
Acc65I G'GTAC_C
Cuts at: 3493 3493
Size: 6172
AclI AA'CG_TT
Cuts at: 1563 4299 4672 1563
Size: 2736 373 3063
Fragments arranged by size:
3063 2736 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2021 3225 4235 5619 5887 1632
Size: 389 1204 1010 1384 268 1917
Fragments arranged by size:
1917 1384 1204 1010 389 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5975
AflIII A'CryG_T
Cuts at: 1203 2103 5071 6048 1203
Size: 900 2968 977 1327
Fragments arranged by size:
2968 1327 977 900
AgeI A'CCGG_T
Cuts at: 5003 5003
Size: 6172
AhdI GACnn_n'nnGTC
Cuts at: 4901 4901
Size: 6172
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6172
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6172
AlwNI CAG_nnn'CTG
Cuts at: 2146 3173 3915 5487 2146
Size: 1027 742 1572 2831
Fragments arranged by size:
2831 1572 1027 742
ApaLI G'TGCA_C
Cuts at: 4229 5385 4229
Size: 1156 5016
ApoI r'AATT_y
Cuts at: 1493 2402 2912 2938 3145 3331 3481 3707 3859
Size: 909 510 26 207 186 150 226 152
Cuts at: 3859 5968 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 909 510 226 207 186 152
150 26
AseI AT'TA_AT
Cuts at: 160 1032 2838 3408 4726 160
Size: 872 1806 570 1318 1606
Fragments arranged by size:
1806 1606 1318 872 570
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
AvaI C'yCGr_G
Cuts at: 1870 1943 3497 1870
Size: 73 1554 4545
Fragments arranged by size:
4545 1554 73
AvrII C'CTAG_G
Cuts at: 3230 3230
Size: 6172
BamHI G'GATC_C
Cuts at: 3502 3869 3502
Size: 367 5805
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3426 3493 4948 618
Size: 325 217 627 1639 67 1455 1842
Fragments arranged by size:
1842 1639 1455 627 325 217 67
BanII G_rGCy'C
Cuts at: 1391 3491 1391
Size: 2100 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5201
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4363 4397 1903
Size: 34 2426 34 3678
Fragments arranged by size:
3678 2426 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4064 5274 4064
Size: 1210 4962
BclI T'GATC_A
Cuts at: 1548 2820 1548
Size: 1272 4900
BfrBI ATG'CAT
Cuts at: 2419 2419
Size: 6172
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4783 136
Size: 107 122 71 631 605 3111 1525
Fragments arranged by size:
3111 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2851 6167 2851
Size: 3316 2856
BlpI GC'TnA_GC
Cuts at: 2601 3589 2601
Size: 988 5184
Bme1580I G_kGCm'C
Cuts at: 1790 4233 5389 1790
Size: 2443 1156 2573
Fragments arranged by size:
2573 2443 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6172
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3368 3903 4861
Size: 416 53 83 399 24 1951 535 958
Cuts at: 4861 442
Size: 1753
Fragments arranged by size:
1951 1753 958 535 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6172
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2674 2674
Size: 6172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2460 3416 4832 1239
Size: 968 253 956 1416 2579
Fragments arranged by size:
2579 1416 968 956 253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4297 5162 5460 4297
Size: 865 298 5009
Fragments arranged by size:
5009 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2318 2956 4835 882
Size: 617 819 638 1879 2219
Fragments arranged by size:
2219 1879 819 638 617
BsaAI yAC'GTr
Cuts at: 493 1204 3963 493
Size: 711 2759 2702
Fragments arranged by size:
2759 2702 711
BsaBI GATnn'nnATC
Cuts at: 2487 6166 2487
Size: 3679 2493
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3979 4361 4996 275
Size: 53 83 186 936 2446 382 635 1451
Fragments arranged by size:
2446 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2131 4605 5003 5277 5424 5835 2131
Size: 2474 398 274 147 411 2468
Fragments arranged by size:
2474 2468 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2075 2105 1178
Size: 30 165 30 672 30 5245
Fragments arranged by size:
5245 672 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 1186
Size: 871 5301
BseYI C'CCAG_C
Cuts at: 2231 2745 5375 5871 2231
Size: 514 2630 496 2532
Fragments arranged by size:
2630 2532 514 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5599
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3622 4383 5411 1540
Size: 275 186 1621 761 1028 2301
Fragments arranged by size:
2301 1621 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2607 3417 4233 4318 5389 2607
Size: 810 816 85 1071 3390
Fragments arranged by size:
3390 1071 816 810 85
BsmI GAATG_Cn'
Cuts at: 3683 3776 3683
Size: 93 6079
BsmBI CGTCTCn'nnnn_
Cuts at: 2282 2282
Size: 6172
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2607 3417 3491 4233 4318 5389 1391
Size: 399 817 810 74 742 85 1071 2174
Fragments arranged by size:
2174 1071 817 810 742 399 85 74
BspEI T'CCGG_A
Cuts at: 5835 5835
Size: 6172
BspHI T'CATG_A
Cuts at: 2584 4061 5791 2584
Size: 1477 1730 2965
Fragments arranged by size:
2965 1730 1477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3527 844
Size: 2683 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4059 1394
Size: 429 2236 3507
Fragments arranged by size:
3507 2236 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2518 4667 4841 65
Size: 1183 1270 2149 174 1396
Fragments arranged by size:
2149 1396 1270 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4816 5003 1700
Size: 110 3006 187 2869
Fragments arranged by size:
3006 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6172
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6172
BssSI C'ACGA_G
Cuts at: 4232 5244 4232
Size: 1012 5160
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6172
BstBI TT'CG_AA
Cuts at: 4976 4976
Size: 6172
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6172
BstYI r'GATC_y
Cuts at: 1972 2314 2851 3047 3502 3869 4254 4271 5723
Size: 342 537 196 455 367 385 17 1452
Cuts at: 5723 5809 5821 6167 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 537 455 385 367 346 342
196 86 17 12
BstZ17I GTA'TAC
Cuts at: 2458 2458
Size: 6172
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4681
Fragments arranged by size:
4681 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2405 487
Size: 683 56 1179 4254
Fragments arranged by size:
4254 1179 683 56
BtsI GCAGTG_nn'
Cuts at: 2027 3298 3688 4482 4502 2027
Size: 1271 390 794 20 3697
Fragments arranged by size:
3697 1271 794 390 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6172
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6137
DraI TTT'AAA
Cuts at: 2859 3476 2859
Size: 617 5555
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5179 809
Size: 507 3863 1802
Fragments arranged by size:
3863 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2706 3619 4508
Size: 54 1425 50 275 18 876 913 889
Cuts at: 4508 6137 8
Size: 1629 43
Fragments arranged by size:
1629 1425 913 889 876 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3619 1537
Size: 275 1807 4090
Fragments arranged by size:
4090 1807 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3103 4102 1353
Size: 1750 999 3423
Fragments arranged by size:
3423 1750 999
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2078 4759 5133 5279 387
Size: 1064 138 489 2681 374 146 1280
Fragments arranged by size:
2681 1280 1064 489 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2021 2207 2460 3225 3416 4235 4832
Size: 393 389 186 253 765 191 819 597
Cuts at: 4832 5619 5887 1239
Size: 787 268 1524
Fragments arranged by size:
1524 819 787 765 597 393 389 268
253 191 186
EcoRI G'AATT_C
Cuts at: 3331 3481 3859 3331
Size: 150 378 5644
Fragments arranged by size:
5644 378 150
EcoRV GAT'ATC
Cuts at: 3135 3135
Size: 6172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3165 3485 3165
Size: 320 5852
FspI TGC'GCA
Cuts at: 4678 4678
Size: 6172
HaeII r_GCGC'y
Cuts at: 1678 2509 5319 1678
Size: 831 2810 2531
Fragments arranged by size:
2810 2531 831
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2075 2107 4813 4845 4887
Size: 32 135 32 503 32 2706 32 42
Cuts at: 4887 4919 1373
Size: 32 2626
Fragments arranged by size:
2706 2626 503 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3516 3762 669
Size: 988 281 1578 246 3079
Fragments arranged by size:
3079 1578 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6172
HpaI GTT'AAC
Cuts at: 3762 3762
Size: 6172
KpnI G_GTAC'C
Cuts at: 3497 3497
Size: 6172
MfeI C'AATT_G
Cuts at: 3771 3771
Size: 6172
MluI A'CGCG_T
Cuts at: 2103 6048 2103
Size: 3945 2227
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5286 5470 1305
Size: 3981 184 2007
Fragments arranged by size:
3981 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4350
Fragments arranged by size:
4350 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2979 4130 4489 4648 518
Size: 648 1813 1151 359 159 2042
Fragments arranged by size:
2042 1813 1151 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2185 3588 4265 5413 5658 1606
Size: 579 1403 677 1148 245 2120
Fragments arranged by size:
2120 1403 1148 677 579 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4681
Fragments arranged by size:
4681 939 552
NdeI CA'TA_TG
Cuts at: 387 2415 387
Size: 2028 4144
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6172
NotI GC'GGCC_GC
Cuts at: 3619 3619
Size: 6172
NsiI A_TGCA'T
Cuts at: 2421 2421
Size: 6172
NspI r_CATG'y
Cuts at: 1132 1229 2351 2834 3530 5075 1132
Size: 97 1122 483 696 1545 2229
Fragments arranged by size:
2229 1545 1122 696 483 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6172
PciI A'CATG_T
Cuts at: 5071 5071
Size: 6172
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6172
PmeI GTTT'AAAC
Cuts at: 3476 3476
Size: 6172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4241 4273 5754 5786 4241
Size: 32 1481 32 4627
Fragments arranged by size:
4627 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6172
PsiI TTA'TAA
Cuts at: 3742 3742
Size: 6172
PstI C_TGCA'G
Cuts at: 830 3524 830
Size: 2694 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6172
PvuII CAG'CTG
Cuts at: 2185 2185
Size: 6172
SalI G'TCGA_C
Cuts at: 1936 3514 1936
Size: 1578 4594
SbfI CC_TGCA'GG
Cuts at: 3524 3524
Size: 6172
ScaI AGT'ACT
Cuts at: 4420 6132 4420
Size: 1712 4460
SfcI C'TryA_G
Cuts at: 826 1045 3520 4655 5336 5527 826
Size: 219 2475 1135 681 191 1471
Fragments arranged by size:
2475 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6172
SmaI CCC'GGG
Cuts at: 1872 3499 1872
Size: 1627 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4276 5177 5439 820
Size: 197 926 2333 901 262 1553
Fragments arranged by size:
2333 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3963 493
Size: 3470 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6172
SphI G_CATG'C
Cuts at: 1132 3530 1132
Size: 2398 3774
SspI AAT'ATT
Cuts at: 5 52 4096 5
Size: 47 4044 2081
Fragments arranged by size:
4044 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6172
StyI C'CwwG_G
Cuts at: 513 1065 2004 3053 3230 3611 513
Size: 552 939 1049 177 381 3074
Fragments arranged by size:
3074 1049 939 552 381 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2397 4199 4216 4369 4554 4999 325
Size: 1255 817 1802 17 153 185 445 1498
Fragments arranged by size:
1802 1498 1255 817 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3314 4418 6130
Size: 274 80 33 51 1443 1337 1104 1712
Cuts at: 6130 96
Size: 138
Fragments arranged by size:
1712 1443 1337 1104 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2093 2125 6132 6164 2093
Size: 32 4007 32 2101
Fragments arranged by size:
4007 2101 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6172
XbaI T'CTAG_A
Cuts at: 3508 3508
Size: 6172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2615 3194 2615
Size: 579 5593
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6172
XmaI C'CCGG_G
Cuts at: 1870 3497 1870
Size: 1627 4545
XmnI GAAnn'nnTTC
Cuts at: 2266 3118 3148 4301 2266
Size: 852 30 1153 4137
Fragments arranged by size:
4137 1153 852 30
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3980 276
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
Bpu10I BsiWI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI