Restriction map for
pFN21AE0302
(Circular) (Six-base) MAPSORT of: pFN21AE0302.seq Check: 4893 from: 1 to: 6133
pFN21AE0302 6133 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3943 278
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3476 1937
Size: 1539 4594
Acc65I G'GTAC_C
Cuts at: 2934 3454 2934
Size: 520 5613
AclI AA'CG_TT
Cuts at: 1563 4260 4633 1563
Size: 2697 373 3063
Fragments arranged by size:
3063 2697 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4196 5580 5848 1632
Size: 2564 1384 268 1917
Fragments arranged by size:
2564 1917 1384 268
AfeI AGC'GCT
Cuts at: 2123 2123
Size: 6133
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5936
AflIII A'CryG_T
Cuts at: 1203 2425 5032 6009 1203
Size: 1222 2607 977 1327
Fragments arranged by size:
2607 1327 1222 977
AgeI A'CCGG_T
Cuts at: 4964 4964
Size: 6133
AhdI GACnn_n'nnGTC
Cuts at: 4862 4862
Size: 6133
AleI CACnn'nnGTG
Cuts at: 1166 2057 1166
Size: 891 5242
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6133
AlwNI CAG_nnn'CTG
Cuts at: 2388 3876 5448 2388
Size: 1488 1572 3073
Fragments arranged by size:
3073 1572 1488
ApaLI G'TGCA_C
Cuts at: 2279 4190 5346 2279
Size: 1911 1156 3066
Fragments arranged by size:
3066 1911 1156
ApoI r'AATT_y
Cuts at: 1493 3442 3668 3820 5929 1493
Size: 1949 226 152 2109 1697
Fragments arranged by size:
2109 1949 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4687 160
Size: 872 3655 1606
Fragments arranged by size:
3655 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
AvaI C'yCGr_G
Cuts at: 1870 1943 2107 3096 3104 3458 1870
Size: 73 164 989 8 354 4545
Fragments arranged by size:
4545 989 354 164 73 8
BamHI G'GATC_C
Cuts at: 2730 2854 3463 3830 2730
Size: 124 609 367 5033
Fragments arranged by size:
5033 609 367 124
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2934 3454 4909 618
Size: 325 217 627 1147 520 1455 1842
Fragments arranged by size:
1842 1455 1147 627 520 325 217
BanII G_rGCy'C
Cuts at: 1391 2066 2078 2114 2653 3218 3296 3452 1391
Size: 675 12 36 539 565 78 156 4072
Fragments arranged by size:
4072 675 565 539 156 78 36 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2037 2428 2777 928
Size: 971 138 391 349 4284
Fragments arranged by size:
4284 971 391 349 138
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2003 2037 2878 2912 4324 4358 1903
Size: 34 66 34 841 34 1412 34 3678
Fragments arranged by size:
3678 1412 841 66 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2303 4025 5235 2303
Size: 1722 1210 3201
Fragments arranged by size:
3201 1722 1210
BclI T'GATC_A
Cuts at: 1548 2259 3321 1548
Size: 711 1062 4360
Fragments arranged by size:
4360 1062 711
BfrBI ATG'CAT
Cuts at: 3165 3165
Size: 6133
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4744 136
Size: 107 122 71 631 605 3072 1525
Fragments arranged by size:
3072 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6128 6128
Size: 6133
BlpI GC'TnA_GC
Cuts at: 3550 3550
Size: 6133
Bme1580I G_kGCm'C
Cuts at: 1790 2283 4194 5350 1790
Size: 493 1911 1156 2573
Fragments arranged by size:
2573 1911 1156 493
BmgBI CAC'GTC
Cuts at: 1318 2059 2426 1318
Size: 741 367 5025
Fragments arranged by size:
5025 741 367
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2425 3864 4822
Size: 416 53 83 399 24 1008 1439 958
Cuts at: 4822 442
Size: 1753
Fragments arranged by size:
1753 1439 1008 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3035 3281 4793 1239
Size: 1796 246 1512 2579
Fragments arranged by size:
2579 1796 1512 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4258 5123 5421 4258
Size: 865 298 4970
Fragments arranged by size:
4970 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4796 882
Size: 617 3297 2219
Fragments arranged by size:
3297 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3924 493
Size: 711 2720 2702
Fragments arranged by size:
2720 2702 711
BsaBI GATnn'nnATC
Cuts at: 3077 3326 6127 3077
Size: 249 2801 3083
Fragments arranged by size:
3083 2801 249
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2570 3315 3940 4322
Size: 53 83 186 936 1037 745 625 382
Cuts at: 4322 4957 275
Size: 635 1451
Fragments arranged by size:
1451 1037 936 745 635 625 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2400 4566 4964 5238 5385 5796 2400
Size: 2166 398 274 147 411 2737
Fragments arranged by size:
2737 2166 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2033 2063 2084 2087 2093
Size: 30 165 30 630 30 21 3 6
Cuts at: 2093 2114 2117 2585 2615 1178
Size: 21 3 468 30 4696
Fragments arranged by size:
4696 630 468 165 30 30 30 30
21 21 6 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2084 2092 1186
Size: 898 8 5227
Fragments arranged by size:
5227 898 8
BseYI C'CCAG_C
Cuts at: 2454 2458 2741 5336 5832 2454
Size: 4 283 2595 496 2755
Fragments arranged by size:
2755 2595 496 283 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5560
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3583 4344 5372 1540
Size: 275 186 1582 761 1028 2301
Fragments arranged by size:
2301 1582 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2283 2311 3111 4194 4279 5350 2283
Size: 28 800 1083 85 1071 3066
Fragments arranged by size:
3066 1083 1071 800 85 28
BsmI GAATG_Cn'
Cuts at: 3644 3737 3644
Size: 93 6040
BsmBI CGTCTCn'nnnn_
Cuts at: 2549 2549
Size: 6133
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2078 2114 2283 2311 2653 3111
Size: 399 276 12 36 169 28 342 458
Cuts at: 3111 3218 3296 3452 4194 4279 5350 1391
Size: 107 78 156 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 458 399 342 276 169
156 107 85 78 36 28 12
BspEI T'CCGG_A
Cuts at: 2400 5796 2400
Size: 3396 2737
BspHI T'CATG_A
Cuts at: 2703 4022 5752 2703
Size: 1319 1730 3084
Fragments arranged by size:
3084 1730 1319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2946 2988 3488 844
Size: 2102 42 500 3489
Fragments arranged by size:
3489 2102 500 42
BsrBI CCG'CTC
Cuts at: 1394 1823 3256 4020 1394
Size: 429 1433 764 3507
Fragments arranged by size:
3507 1433 764 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3400 4628 4802 65
Size: 1183 2152 1228 174 1396
Fragments arranged by size:
2152 1396 1228 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4777 4964 1700
Size: 110 2967 187 2869
Fragments arranged by size:
2967 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6133
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6133
BssSI C'ACGA_G
Cuts at: 4193 5205 4193
Size: 1012 5121
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6133
BstBI TT'CG_AA
Cuts at: 4937 4937
Size: 6133
BstEII G'GTnAC_C
Cuts at: 1184 3265 1184
Size: 2081 4052
BstXI CCAn_nnnn'nTGG
Cuts at: 2126 2126
Size: 6133
BstYI r'GATC_y
Cuts at: 1972 2730 2854 3030 3463 3830 4215 4232 5684
Size: 758 124 176 433 367 385 17 1452
Cuts at: 5684 5770 5782 6128 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 758 433 385 367 346 176
124 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2067 2067
Size: 6133
BtgI C'CryG_G
Cuts at: 513 1065 2052 2577 513
Size: 552 987 525 4069
Fragments arranged by size:
4069 987 552 525
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5394
Fragments arranged by size:
5394 683 56
BtsI GCAGTG_nn'
Cuts at: 2281 3649 4443 4463 2281
Size: 1368 794 20 3951
Fragments arranged by size:
3951 1368 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6098
DraI TTT'AAA
Cuts at: 2503 3437 2503
Size: 934 5199
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5140 809
Size: 507 3824 1802
Fragments arranged by size:
3824 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2274 2520 2673
Size: 54 1425 50 275 18 444 246 153
Cuts at: 2673 2691 3171 3580 4469 6098 8
Size: 18 480 409 889 1629 43
Fragments arranged by size:
1629 1425 889 480 444 409 275 246
153 54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3580 1537
Size: 275 1768 4090
Fragments arranged by size:
4090 1768 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2789 4063 1353
Size: 1436 1274 3423
Fragments arranged by size:
3423 1436 1274
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4720 5094 5240 387
Size: 1064 138 3131 374 146 1280
Fragments arranged by size:
3131 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3035 3281 4196 4793 5580 5848 1239
Size: 393 1403 246 915 597 787 268 1524
Fragments arranged by size:
1524 1403 915 787 597 393 268 246
EcoO109I rG'GnC_Cy
Cuts at: 2243 3357 2243
Size: 1114 5019
EcoRI G'AATT_C
Cuts at: 3442 3820 3442
Size: 378 5755
FspI TGC'GCA
Cuts at: 3140 4639 3140
Size: 1499 4634
FspAI rTGC'GCAy
Cuts at: 3140 3140
Size: 6133
HaeII r_GCGC'y
Cuts at: 1678 2125 3104 3234 5280 1678
Size: 447 979 130 2046 2531
Fragments arranged by size:
2531 2046 979 447 130
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2063 2065 2095 2764
Size: 32 135 32 461 30 2 30 669
Cuts at: 2764 2796 2940 2972 4774 4806 4848 4880 1373
Size: 32 144 32 1802 32 42 32 2626
Fragments arranged by size:
2626 1802 669 461 144 135 42 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3477 3723 669
Size: 988 281 1539 246 3079
Fragments arranged by size:
3079 1539 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6133
HpaI GTT'AAC
Cuts at: 3723 3723
Size: 6133
KpnI G_GTAC'C
Cuts at: 2938 3458 2938
Size: 520 5613
MfeI C'AATT_G
Cuts at: 3732 3732
Size: 6133
MluI A'CGCG_T
Cuts at: 6009 6009
Size: 6133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2061 5247 5431 1305
Size: 756 3186 184 2007
Fragments arranged by size:
3186 2007 756 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2675 2693 3173 10
Size: 54 1425 343 843 18 480 2970
Fragments arranged by size:
2970 1425 843 480 343 54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 2237 2550 2684 2822 4091 4450
Size: 648 891 180 313 134 138 1269 359
Cuts at: 4450 4609 518
Size: 159 2042
Fragments arranged by size:
2042 1269 891 648 359 313 180 159
138 134
MspA1I CmG'CkG
Cuts at: 1606 2458 3549 4226 5374 5619 1606
Size: 852 1091 677 1148 245 2120
Fragments arranged by size:
2120 1148 1091 852 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2577 513
Size: 552 1512 4069
Fragments arranged by size:
4069 1512 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6133
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6133
NotI GC'GGCC_GC
Cuts at: 3580 3580
Size: 6133
NruI TCG'CGA
Cuts at: 2587 2587
Size: 6133
NsiI A_TGCA'T
Cuts at: 3167 3167
Size: 6133
NspI r_CATG'y
Cuts at: 1132 1229 2599 3165 3491 5036 1132
Size: 97 1370 566 326 1545 2229
Fragments arranged by size:
2229 1545 1370 566 326 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6133
PciI A'CATG_T
Cuts at: 5032 5032
Size: 6133
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2991 1108
Size: 1883 4250
PfoI T'CCnGG_A
Cuts at: 3258 3258
Size: 6133
PmeI GTTT'AAAC
Cuts at: 3437 3437
Size: 6133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4202 4234 5715 5747 4202
Size: 32 1481 32 4588
Fragments arranged by size:
4588 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2243 3357 2243
Size: 1114 5019
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6133
PsiI TTA'TAA
Cuts at: 3703 3703
Size: 6133
PstI C_TGCA'G
Cuts at: 830 3485 830
Size: 2655 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6133
PvuII CAG'CTG
Cuts at: 2458 2458
Size: 6133
SalI G'TCGA_C
Cuts at: 1936 3475 1936
Size: 1539 4594
SanDI GG'GwC_CC
Cuts at: 2243 2243
Size: 6133
SbfI CC_TGCA'GG
Cuts at: 3485 3485
Size: 6133
ScaI AGT'ACT
Cuts at: 4381 6093 4381
Size: 1712 4421
SexAI A'CCwGG_T
Cuts at: 3195 3195
Size: 6133
SfcI C'TryA_G
Cuts at: 826 1045 2044 3481 4616 5297 5488 826
Size: 219 999 1437 1135 681 191 1471
Fragments arranged by size:
1471 1437 1135 999 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6133
SmaI CCC'GGG
Cuts at: 1872 2109 3460 1872
Size: 237 1351 4545
Fragments arranged by size:
4545 1351 237
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4237 5138 5400 820
Size: 197 926 2294 901 262 1553
Fragments arranged by size:
2294 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3924 493
Size: 3431 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6133
SphI G_CATG'C
Cuts at: 1132 3491 1132
Size: 2359 3774
SspI AAT'ATT
Cuts at: 5 52 4057 5
Size: 47 4005 2081
Fragments arranged by size:
4005 2081 47
StuI AGG'CCT
Cuts at: 1517 2088 1517
Size: 571 5562
StyI C'CwwG_G
Cuts at: 513 1065 2577 2754 2765 2959 3572 513
Size: 552 1512 177 11 194 613 3074
Fragments arranged by size:
3074 1512 613 552 194 177 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2468 4160 4177 4330 4515 4960 325
Size: 1255 888 1692 17 153 185 445 1498
Fragments arranged by size:
1692 1498 1255 888 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3018 4379 6091
Size: 274 80 33 51 1443 1041 1361 1712
Cuts at: 6091 96
Size: 138
Fragments arranged by size:
1712 1443 1361 1041 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6093 6125 6093
Size: 32 6101
Tth111I GACn'n_nGTC
Cuts at: 1316 3197 1316
Size: 1881 4252
XbaI T'CTAG_A
Cuts at: 3469 3469
Size: 6133
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6133
XmaI C'CCGG_G
Cuts at: 1870 2107 3458 1870
Size: 237 1351 4545
Fragments arranged by size:
4545 1351 237
XmnI GAAnn'nnTTC
Cuts at: 4262 4262
Size: 6133
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3941 276
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NruI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI KasI NaeI NarI NgoMIV PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI
SfoI SrfI SwaI XcmI