Restriction map for
pFN21AE0298
(Circular) (Six-base) MAPSORT of: pFN21AE0298.seq Check: 6408 from: 1 to: 12859
pFN21AE0298 12859 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 7636 9857 9865 7636
Size: 2221 8 10630
Fragments arranged by size:
10630 2221 8
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 10669 278
Size: 53 83 186 936 9133 2468
Fragments arranged by size:
9133 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5330 6605 7201 8880 10202 1937
Size: 3393 1275 596 1679 1322 4594
Fragments arranged by size:
4594 3393 1679 1322 1275 596
Acc65I G'GTAC_C
Cuts at: 9223 10180 9223
Size: 957 11902
AclI AA'CG_TT
Cuts at: 1563 6510 10986 11359 1563
Size: 4947 4476 373 3063
Fragments arranged by size:
4947 4476 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2201 2385 2400 3776 4704 5117 5162 5190
Size: 569 184 15 1376 928 413 45 28
Cuts at: 5190 5606 6693 7209 7620 7875 8654 9245 9419
Size: 416 1087 516 411 255 779 591 174
Cuts at: 9419 10019 10032 10093 10922 12306 12574 1632
Size: 600 13 61 829 1384 268 1917
Fragments arranged by size:
1917 1384 1376 1087 928 829 779 600
591 569 516 416 413 411 268 255
184 174 61 45 28 15 13
AflII C'TTAA_G
Cuts at: 820 1017 3721 7846 8071 820
Size: 197 2704 4125 225 5608
Fragments arranged by size:
5608 4125 2704 225 197
AflIII A'CryG_T
Cuts at: 1203 3825 5656 11758 12735 1203
Size: 2622 1831 6102 977 1327
Fragments arranged by size:
6102 2622 1831 1327 977
AgeI A'CCGG_T
Cuts at: 11690 11690
Size: 12859
AhdI GACnn_n'nnGTC
Cuts at: 11588 11588
Size: 12859
AleI CACnn'nnGTG
Cuts at: 1166 9251 9344 1166
Size: 8085 93 4681
Fragments arranged by size:
8085 4681 93
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3426 8862 9410 1184
Size: 2242 5436 548 4633
Fragments arranged by size:
5436 4633 2242 548
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3718 3750 4399 4431 9558 9590 3718
Size: 32 649 32 5127 32 6987
Fragments arranged by size:
6987 5127 649 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3413 4556 4759 5497 7795 9577 10602 12174 3413
Size: 1143 203 738 2298 1782 1025 1572 4098
Fragments arranged by size:
4098 2298 1782 1572 1143 1025 738 203
ApaI G_GGCC'C
Cuts at: 5303 5303
Size: 12859
ApaLI G'TGCA_C
Cuts at: 3551 3771 10103 10916 12072 3551
Size: 220 6332 813 1156 4338
Fragments arranged by size:
6332 4338 1156 813 220
ApoI r'AATT_y
Cuts at: 1493 2022 2468 3865 4921 4940 6631 7054 7112
Size: 529 446 1397 1056 19 1691 423 58
Cuts at: 7112 7150 7538 7574 8114 8164 8937 10168 10394
Size: 38 388 36 540 50 773 1231 226
Cuts at: 10394 10546 12655 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1691 1397 1231 1056 773 540
529 446 423 388 226 152 58 50
38 36 19
AseI AT'TA_AT
Cuts at: 160 1032 6117 6818 11413 160
Size: 872 5085 701 4595 1606
Fragments arranged by size:
5085 4595 1606 872 701
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12859
AvaI C'yCGr_G
Cuts at: 1870 1943 5565 6237 10184 1870
Size: 73 3622 672 3947 4545
Fragments arranged by size:
4545 3947 3622 672 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 9430 9463 9430
Size: 33 12826
BamHI G'GATC_C
Cuts at: 3748 5317 9213 10189 10556 3748
Size: 1569 3896 976 367 6051
Fragments arranged by size:
6051 3896 1569 976 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3500 3530 6892 9223 10180
Size: 325 217 627 1713 30 3362 2331 957
Cuts at: 10180 11635 618
Size: 1455 1842
Fragments arranged by size:
3362 2331 1842 1713 1455 957 627 325
217 30
BanII G_rGCy'C
Cuts at: 1391 2648 5303 6864 7425 8987 9709 10178 1391
Size: 1257 2655 1561 561 1562 722 469 4072
Fragments arranged by size:
4072 2655 1562 1561 1257 722 561 469
BbeI G_GCGC'C
Cuts at: 3504 3534 3504
Size: 30 12829
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2766 3480 4719 6642 8583 8819 928
Size: 971 867 714 1239 1923 1941 236 4968
Fragments arranged by size:
4968 1941 1923 1239 971 867 714 236
BbvCI CC'TCA_GC
Cuts at: 6405 6405
Size: 12859
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2143 2177 6148 6182 11050
Size: 34 50 34 122 34 3971 34 4868
Cuts at: 11050 11084 1903
Size: 34 3678
Fragments arranged by size:
4868 3971 3678 122 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8470 10751 11961 8470
Size: 2281 1210 9368
Fragments arranged by size:
9368 2281 1210
BclI T'GATC_A
Cuts at: 1548 5761 1548
Size: 4213 8646
BfrBI ATG'CAT
Cuts at: 2541 2541
Size: 12859
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 9617 11470 136
Size: 107 122 71 631 605 7945 1853 1525
Fragments arranged by size:
7945 1853 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3029 3653 6991 9639 12854 3029
Size: 624 3338 2648 3215 3034
Fragments arranged by size:
3338 3215 3034 2648 624
BlpI GC'TnA_GC
Cuts at: 9018 10276 9018
Size: 1258 11601
Bme1580I G_kGCm'C
Cuts at: 1790 3555 3775 4668 5013 5303 9258 10107 10920
Size: 1765 220 893 345 290 3955 849 813
Cuts at: 10920 12076 1790
Size: 1156 2573
Fragments arranged by size:
3955 2573 1765 1156 893 849 813 345
290 220
BmgBI CAC'GTC
Cuts at: 1318 7333 9984 10117 1318
Size: 6015 2651 133 4060
Fragments arranged by size:
6015 4060 2651 133
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3398 5202 5474
Size: 416 53 83 399 24 1981 1804 272
Cuts at: 5474 6261 6369 10590 11548 442
Size: 787 108 4221 958 1753
Fragments arranged by size:
4221 1981 1804 1753 958 787 416 399
272 108 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 8640 1056
Size: 7584 5275
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2229 2229
Size: 12859
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3312 3829 5617 5896 6481 7680 9099 9390
Size: 2073 517 1788 279 585 1199 1419 291
Cuts at: 9390 9620 9693 10035 11519 1239
Size: 230 73 342 1484 2579
Fragments arranged by size:
2579 2073 1788 1484 1419 1199 585 517
342 291 279 230 73
Bpu10I CC'TnA_GC
Cuts at: 4995 5304 5429 5457 6405 6863 8783 4995
Size: 309 125 28 948 458 1920 9071
Fragments arranged by size:
9071 1920 948 458 309 125 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2482 4256 5659 9063 10984 11849 12147 2482
Size: 1774 1403 3404 1921 865 298 3194
Fragments arranged by size:
3404 3194 1921 1774 1403 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5526 5580 5733 6148 7330 9638 11522
Size: 617 4027 54 153 415 1182 2308 1884
Cuts at: 11522 882
Size: 2219
Fragments arranged by size:
4027 2308 2219 1884 1182 617 415 153
54
BsaAI yAC'GTr
Cuts at: 493 1204 3919 9934 10650 493
Size: 711 2715 6015 716 2702
Fragments arranged by size:
6015 2715 2702 716 711
BsaBI GATnn'nnATC
Cuts at: 2443 5537 6327 12853 2443
Size: 3094 790 6526 2449
Fragments arranged by size:
6526 3094 2449 790
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3501 3531 5078 8448
Size: 53 83 186 936 1968 30 1547 3370
Cuts at: 8448 10666 11048 11683 275
Size: 2218 382 635 1451
Fragments arranged by size:
3370 2218 1968 1547 1451 936 635 382
186 83 53 30
BsaWI w'CCGG_w
Cuts at: 11292 11690 11964 12111 12522 11292
Size: 398 274 147 411 11629
Fragments arranged by size:
11629 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4399 4429 5408 5438 5908
Size: 30 165 30 2996 30 979 30 470
Cuts at: 5908 5938 7950 7980 8806 8836 9784 9814 10033
Size: 30 2012 30 826 30 948 30 219
Cuts at: 10033 10063 1178
Size: 30 3974
Fragments arranged by size:
3974 2996 2012 979 948 826 470 219
165 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3033 3506 4253 4563 4566 4825 5628 9792
Size: 1847 473 747 310 3 259 803 4164
Cuts at: 9792 1186
Size: 4253
Fragments arranged by size:
4253 4164 1847 803 747 473 310 259
3
BseYI C'CCAG_C
Cuts at: 3936 4094 5051 5793 7252 7788 9168 12062 12558
Size: 158 957 742 1459 536 1380 2894 496
Cuts at: 12558 3936
Size: 4237
Fragments arranged by size:
4237 2894 1459 1380 957 742 536 496
158
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2676 6905 8341 9296 1163
Size: 573 940 4229 1436 955 4726
Fragments arranged by size:
4726 4229 1436 955 940 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 10309 11070 12098 1540
Size: 275 186 8308 761 1028 2301
Fragments arranged by size:
8308 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2630 3555 3775 5001 6584 6864 8789 9024 9709
Size: 925 220 1226 1583 280 1925 235 685
Cuts at: 9709 10107 10920 11005 12076 2630
Size: 398 813 85 1071 3413
Fragments arranged by size:
3413 1925 1583 1226 1071 925 813 685
398 280 235 220 85
BsmI GAATG_Cn'
Cuts at: 2543 2588 2965 5194 6388 6739 7093 9163 9562
Size: 45 377 2229 1194 351 354 2070 399
Cuts at: 9562 10370 10463 2543
Size: 808 93 4939
Fragments arranged by size:
4939 2229 2070 1194 808 399 377 354
351 93 45
BsmBI CGTCTCn'nnnn_
Cuts at: 8455 8455
Size: 12859
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2630 2648 3555 3775 4668 5001 5013
Size: 399 840 18 907 220 893 333 12
Cuts at: 5013 5303 6584 6864 7425 8789 8987 9024 9258
Size: 290 1281 280 561 1364 198 37 234
Cuts at: 9258 9709 10107 10178 10920 11005 12076 1391
Size: 451 398 71 742 85 1071 2174
Fragments arranged by size:
2174 1364 1281 1071 907 893 840 742
561 451 399 398 333 290 280 234
220 198 85 71 37 18 12
BspEI T'CCGG_A
Cuts at: 12522 12522
Size: 12859
BspHI T'CATG_A
Cuts at: 10748 12478 10748
Size: 1730 11129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4564 7636 9235 9562 9857 9865 10214 844
Size: 3720 3072 1599 327 295 8 349 3489
Fragments arranged by size:
3720 3489 3072 1599 349 327 295 8
BsrBI CCG'CTC
Cuts at: 1394 1823 3282 4243 10746 1394
Size: 429 1459 961 6503 3507
Fragments arranged by size:
6503 3507 1459 961 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2616 2838 4187 7269 9653 11354 11528
Size: 1183 1368 222 1349 3082 2384 1701 174
Cuts at: 11528 65
Size: 1396
Fragments arranged by size:
3082 2384 1701 1396 1368 1349 1183 222
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 11503 11690 1700
Size: 110 9693 187 2869
Fragments arranged by size:
9693 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 6340 7667 96
Size: 6244 1327 5288
Fragments arranged by size:
6244 5288 1327
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12859
BssSI C'ACGA_G
Cuts at: 3434 10919 11931 3434
Size: 7485 1012 4362
Fragments arranged by size:
7485 4362 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3550 4556 6745 6847 6892 9738 794
Size: 2756 1006 2189 102 45 2846 3915
Fragments arranged by size:
3915 2846 2756 2189 1006 102 45
BstBI TT'CG_AA
Cuts at: 7618 11663 7618
Size: 4045 8814
BstEII G'GTnAC_C
Cuts at: 1184 4089 9810 9869 1184
Size: 2905 5721 59 4174
Fragments arranged by size:
5721 4174 2905 59
BstXI CCAn_nnnn'nTGG
Cuts at: 4790 4790
Size: 12859
BstYI r'GATC_y
Cuts at: 1972 2444 3029 3653 3748 5317 6472 6766 6991
Size: 472 585 624 95 1569 1155 294 225
Cuts at: 6991 9213 9639 10189 10556 10941 10958 12410 12496
Size: 2222 426 550 367 385 17 1452 86
Cuts at: 12496 12508 12854 1972
Size: 12 346 1977
Fragments arranged by size:
2222 1977 1569 1452 1155 624 585 550
472 426 385 367 346 294 225 95
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 9615 9804 10030 9615
Size: 189 226 12444
Fragments arranged by size:
12444 226 189
BtgI C'CryG_G
Cuts at: 513 1065 3534 3744 4310 5313 7275 10085 513
Size: 552 2469 210 566 1003 1962 2810 3287
Fragments arranged by size:
3287 2810 2469 1962 1003 566 552 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 10140 487
Size: 683 56 8914 3206
Fragments arranged by size:
8914 3206 683 56
BtsI GCAGTG_nn'
Cuts at: 2583 2997 6393 8353 9394 10375 11169 11189 2583
Size: 414 3396 1960 1041 981 794 20 4253
Fragments arranged by size:
4253 3396 1960 1041 981 794 414 20
ClaI AT'CG_AT
Cuts at: 1554 6012 1554
Size: 4458 8401
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 7923 7936 7958 7971 8092 8127 529
Size: 35 7359 13 22 13 121 35 5261
Fragments arranged by size:
7359 5261 121 35 35 22 13 13
DraI TTT'AAA
Cuts at: 3270 4369 4392 6309 10163 3270
Size: 1099 23 1917 3854 5966
Fragments arranged by size:
5966 3854 1917 1099 23
DraIII CAC_nnn'GTG
Cuts at: 2654 2654
Size: 12859
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8042 8696 11866 809
Size: 507 6726 654 3170 1802
Fragments arranged by size:
6726 3170 1802 654 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4221 9412 10306
Size: 54 1425 50 275 18 2391 5191 894
Cuts at: 10306 11195 12824 8
Size: 889 1629 43
Fragments arranged by size:
5191 2391 1629 1425 894 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 10306 1537
Size: 275 8494 4090
Fragments arranged by size:
8494 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2220 2238 2551 4723 6375 6730 8223 8251
Size: 867 18 313 2172 1652 355 1493 28
Cuts at: 8251 10789 1353
Size: 2538 3423
Fragments arranged by size:
3423 2538 2172 1652 1493 867 355 313
28 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8333 11446 11820 11966 387
Size: 1064 138 6744 3113 374 146 1280
Fragments arranged by size:
6744 3113 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 6862 9707 6862
Size: 2845 10014
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2385 2400 3312 3776 3829 4704
Size: 393 569 184 15 912 464 53 875
Cuts at: 4704 5117 5162 5190 5606 5617 5896 6481 6693
Size: 413 45 28 416 11 279 585 212
Cuts at: 6693 7209 7620 7680 7875 8654 9099 9245 9390
Size: 516 411 60 195 779 445 146 145
Cuts at: 9390 9419 9620 9693 10019 10032 10035 10093 10922
Size: 29 201 73 326 13 3 58 829
Cuts at: 10922 11519 12306 12574 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 912 875 829 787 779 597 585
569 516 464 445 416 413 411 393
326 279 268 212 201 195 184 146
145 73 60 58 53 45 29 28
15 13 11 3
EcoO109I rG'GnC_Cy
Cuts at: 3787 4649 5055 5299 5300 6401 6867 8674 8892
Size: 862 406 244 1 1101 466 1807 218
Cuts at: 8892 3787
Size: 7754
Fragments arranged by size:
7754 1807 1101 862 466 406 244 218
1
EcoRI G'AATT_C
Cuts at: 3865 8114 10168 10546 3865
Size: 4249 2054 378 6178
Fragments arranged by size:
6178 4249 2054 378
EcoRV GAT'ATC
Cuts at: 4248 6538 4248
Size: 2290 10569
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2422 4493 5577 7976 8053 2422
Size: 2071 1084 2399 77 7228
Fragments arranged by size:
7228 2399 2071 1084 77
FspI TGC'GCA
Cuts at: 11365 11365
Size: 12859
HaeII r_GCGC'y
Cuts at: 1678 2457 3504 3534 4623 7920 8762 12006 1678
Size: 779 1047 30 1089 3297 842 3244 2531
Fragments arranged by size:
3297 3244 2531 1089 1047 842 779 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4232 4264 4663 4695 5302
Size: 32 135 32 2660 32 399 32 607
Cuts at: 5302 5334 6815 6847 6976 7008 7505 7537 8806
Size: 32 1481 32 129 32 497 32 1269
Cuts at: 8806 8838 9784 9816 10033 10065 11500 11532 11574
Size: 32 946 32 217 32 1435 32 42
Cuts at: 11574 11606 1373
Size: 32 2626
Fragments arranged by size:
2660 2626 1481 1435 1269 946 607 497
399 217 135 129 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4155 5331 7202 7745 10203 10449
Size: 988 281 2217 1176 1871 543 2458 246
Cuts at: 10449 669
Size: 3079
Fragments arranged by size:
3079 2458 2217 1871 1176 988 543 281
246
HindIII A'AGCT_T
Cuts at: 748 3598 4253 4272 748
Size: 2850 655 19 9335
Fragments arranged by size:
9335 2850 655 19
HpaI GTT'AAC
Cuts at: 10449 10449
Size: 12859
KasI G'GCGC_C
Cuts at: 3500 3530 3500
Size: 30 12829
KpnI G_GTAC'C
Cuts at: 9227 10184 9227
Size: 957 11902
MfeI C'AATT_G
Cuts at: 6686 10458 6686
Size: 3772 9087
MluI A'CGCG_T
Cuts at: 12735 12735
Size: 12859
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2910 3831 6754 7422 9544 11973 12157 1305
Size: 1605 921 2923 668 2122 2429 184 2007
Fragments arranged by size:
2923 2429 2122 2007 1605 921 668 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4223 10
Size: 54 1425 343 2391 8646
Fragments arranged by size:
8646 2391 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2862 4805 7086 7274 8337 8771 9251
Size: 648 1696 1943 2281 188 1063 434 480
Cuts at: 9251 9344 10817 11176 11335 518
Size: 93 1473 359 159 2042
Fragments arranged by size:
2281 2042 1943 1696 1473 1063 648 480
434 359 188 159 93
MspA1I CmG'CkG
Cuts at: 1606 4312 6050 7511 7795 9017 9041 9826 10087
Size: 2706 1738 1461 284 1222 24 785 261
Cuts at: 10087 10275 10952 12100 12345 1606
Size: 188 677 1148 245 2120
Fragments arranged by size:
2706 2120 1738 1461 1222 1148 785 677
284 261 245 188 24
NarI GG'CG_CC
Cuts at: 3501 3531 3501
Size: 30 12829
NcoI C'CATG_G
Cuts at: 513 1065 3534 3744 5313 7275 513
Size: 552 2469 210 1569 1962 6097
Fragments arranged by size:
6097 2469 1962 1569 552 210
NdeI CA'TA_TG
Cuts at: 387 2799 387
Size: 2412 10447
NheI G'CTAG_C
Cuts at: 1052 8636 1052
Size: 7584 5275
NotI GC'GGCC_GC
Cuts at: 10306 10306
Size: 12859
NsiI A_TGCA'T
Cuts at: 2543 2543
Size: 12859
NspI r_CATG'y
Cuts at: 1132 1229 3249 3829 5660 6260 8311 9741 10217
Size: 97 2020 580 1831 600 2051 1430 476
Cuts at: 10217 11762 1132
Size: 1545 2229
Fragments arranged by size:
2229 2051 2020 1831 1545 1430 600 580
476 97
PasI CC'CwG_GG
Cuts at: 1348 3832 8754 1348
Size: 2484 4922 5453
Fragments arranged by size:
5453 4922 2484
PciI A'CATG_T
Cuts at: 3825 5656 11758 3825
Size: 1831 6102 4926
Fragments arranged by size:
6102 4926 1831
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3058 3413 7795 1108
Size: 1950 355 4382 6172
Fragments arranged by size:
6172 4382 1950 355
PfoI T'CCnGG_A
Cuts at: 5490 5912 6073 5490
Size: 422 161 12276
Fragments arranged by size:
12276 422 161
PmeI GTTT'AAAC
Cuts at: 10163 10163
Size: 12859
PmlI CAC'GTG
Cuts at: 9934 9934
Size: 12859
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4399 4431 10928 10960 12441 12473 4399
Size: 32 6497 32 1481 32 4785
Fragments arranged by size:
6497 4785 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3787 4649 5055 8674 8892 3787
Size: 862 406 3619 218 7754
Fragments arranged by size:
7754 3619 862 406 218
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 12859
PsiI TTA'TAA
Cuts at: 2982 9192 10429 2982
Size: 6210 1237 5412
Fragments arranged by size:
6210 5412 1237
PspOMI G'GGCC_C
Cuts at: 5299 5299
Size: 12859
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 9304 9336 9304
Size: 32 12827
PstI C_TGCA'G
Cuts at: 830 2418 3546 3661 4138 5025 5385 6480 6927
Size: 1588 1128 115 477 887 360 1095 447
Cuts at: 6927 9016 9573 9868 10211 830
Size: 2089 557 295 343 3478
Fragments arranged by size:
3478 2089 1588 1128 1095 887 557 477
447 360 343 295 115
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12859
PvuII CAG'CTG
Cuts at: 6050 7511 7795 9017 6050
Size: 1461 284 1222 9892
Fragments arranged by size:
9892 1461 1222 284
SacI G_AGCT'C
Cuts at: 6864 9709 6864
Size: 2845 10014
SacII CC_GC'GG
Cuts at: 4313 10088 4313
Size: 5775 7084
SalI G'TCGA_C
Cuts at: 1936 5329 7200 10201 1936
Size: 3393 1871 3001 4594
Fragments arranged by size:
4594 3393 3001 1871
SapI GCTCTTCn'nnn_
Cuts at: 6375 6730 6375
Size: 355 12504
SbfI CC_TGCA'GG
Cuts at: 9868 10211 9868
Size: 343 12516
ScaI AGT'ACT
Cuts at: 8686 11107 12819 8686
Size: 2421 1712 8726
Fragments arranged by size:
8726 2421 1712
SexAI A'CCwGG_T
Cuts at: 4198 9780 4198
Size: 5582 7277
SfcI C'TryA_G
Cuts at: 826 1045 2414 3003 3542 3644 3657 4134 5021
Size: 219 1369 589 539 102 13 477 887
Cuts at: 5021 5041 5381 6476 6923 7416 8877 9012 9104
Size: 20 340 1095 447 493 1461 135 92
Cuts at: 9104 9569 9765 9816 9864 10207 11342 12023 12214
Size: 465 196 51 48 343 1135 681 191
Cuts at: 12214 826
Size: 1471
Fragments arranged by size:
1471 1461 1369 1135 1095 887 681 589
539 493 477 465 447 343 340 219
196 191 135 102 92 51 48 20
13
SfoI GGC'GCC
Cuts at: 3502 3532 3502
Size: 30 12829
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12859
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12859
SmaI CCC'GGG
Cuts at: 1872 10186 1872
Size: 8314 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2497 3721 4235 5674 6237 7846
Size: 197 926 554 1224 514 1439 563 1609
Cuts at: 7846 8071 9078 10963 11864 12126 820
Size: 225 1007 1885 901 262 1553
Fragments arranged by size:
1885 1609 1553 1439 1224 1007 926 901
563 554 514 262 225 197
SnaBI TAC'GTA
Cuts at: 493 10650 493
Size: 10157 2702
SpeI A'CTAG_T
Cuts at: 152 6413 6979 152
Size: 6261 566 6032
Fragments arranged by size:
6261 6032 566
SphI G_CATG'C
Cuts at: 1132 9741 10217 1132
Size: 8609 476 3774
Fragments arranged by size:
8609 3774 476
SspI AAT'ATT
Cuts at: 5 52 8202 9496 10783 5
Size: 47 8150 1294 1287 2081
Fragments arranged by size:
8150 2081 1294 1287 47
StuI AGG'CCT
Cuts at: 1517 3009 5175 9432 9504 1517
Size: 1492 2166 4257 72 4872
Fragments arranged by size:
4872 4257 2166 1492 72
StyI C'CwwG_G
Cuts at: 513 1065 3534 3744 4774 5313 6871 7275 8415
Size: 552 2469 210 1030 539 1558 404 1140
Cuts at: 8415 10298 513
Size: 1883 3074
Fragments arranged by size:
3074 2469 1883 1558 1140 1030 552 539
404 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5854 6360 7802 9809 10886 10903 11056
Size: 1255 4274 506 1442 2007 1077 17 153
Cuts at: 11056 11241 11686 325
Size: 185 445 1498
Fragments arranged by size:
4274 2007 1498 1442 1255 1077 506 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6340 6416 7412
Size: 274 80 33 51 1443 4363 76 996
Cuts at: 7412 7667 8684 11105 12817 96
Size: 255 1017 2421 1712 138
Fragments arranged by size:
4363 2421 1712 1443 1017 996 274 255
138 80 76 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3350 3382 5408 5440 6867 6899 7950 7982 8396
Size: 32 2026 32 1427 32 1051 32 414
Cuts at: 8396 8428 12819 12851 3350
Size: 32 4391 32 3358
Fragments arranged by size:
4391 3358 2026 1427 1051 414 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 9796 1316
Size: 8480 4379
XbaI T'CTAG_A
Cuts at: 8657 10195 8657
Size: 1538 11321
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 9517 9517
Size: 12859
XhoI C'TCGA_G
Cuts at: 1943 6237 1943
Size: 4294 8565
XmaI C'CCGG_G
Cuts at: 1870 10184 1870
Size: 8314 4545
XmnI GAAnn'nnTTC
Cuts at: 4414 4522 5130 5175 8937 10988 4414
Size: 108 608 45 3762 2051 6285
Fragments arranged by size:
6285 3762 2051 608 108 45
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 10667 276
Size: 53 83 186 936 9133 2468
Fragments arranged by size:
9133 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BsiWI BstZ17I EcoNI FseI FspAI
NaeI NgoMIV NruI PacI PspXI RsrII SanDI SfiI
SrfI SwaI