Restriction map for
pFN21AE0297
(Circular) (Six-base) MAPSORT of: pFN21AE0297.seq Check: 2601 from: 1 to: 9463
pFN21AE0297 9463 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3582 5245 5625 6302 3582
Size: 1663 380 677 6743
Fragments arranged by size:
6743 1663 677 380
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2015 7273 278
Size: 53 83 186 936 479 5258 2468
Fragments arranged by size:
5258 2468 936 479 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5466 6806 1937
Size: 3529 1340 4594
Fragments arranged by size:
4594 3529 1340
Acc65I G'GTAC_C
Cuts at: 2220 6784 2220
Size: 4564 4899
AclI AA'CG_TT
Cuts at: 1563 7590 7963 1563
Size: 6027 373 3063
Fragments arranged by size:
6027 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3584 4300 7526 8910 9178 1632
Size: 1952 716 3226 1384 268 1917
Fragments arranged by size:
3226 1952 1917 1384 716 268
AfeI AGC'GCT
Cuts at: 4961 4961
Size: 9463
AflII C'TTAA_G
Cuts at: 820 1017 2477 820
Size: 197 1460 7806
Fragments arranged by size:
7806 1460 197
AflIII A'CryG_T
Cuts at: 1203 2646 4587 6360 8362 9339 1203
Size: 1443 1941 1773 2002 977 1327
Fragments arranged by size:
2002 1941 1773 1443 1327 977
AgeI A'CCGG_T
Cuts at: 4185 8294 4185
Size: 4109 5354
AhdI GACnn_n'nnGTC
Cuts at: 8192 8192
Size: 9463
AleI CACnn'nnGTG
Cuts at: 1166 5226 1166
Size: 4060 5403
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3827 3845 4757 5553 5837 1184
Size: 2643 18 912 796 284 4810
Fragments arranged by size:
4810 2643 912 796 284 18
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5898 5930 6728 6760 5898
Size: 32 798 32 8601
Fragments arranged by size:
8601 798 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2027 2902 3042 4694 5677 6181 6248 6517 7206
Size: 875 140 1652 983 504 67 269 689
Cuts at: 7206 8778 2027
Size: 1572 2712
Fragments arranged by size:
2712 1652 1572 983 875 689 504 269
140 67
ApaI G_GGCC'C
Cuts at: 2262 3985 4068 4341 5151 5360 5791 6322 2262
Size: 1723 83 273 810 209 431 531 5403
Fragments arranged by size:
5403 1723 810 531 431 273 209 83
ApaLI G'TGCA_C
Cuts at: 2252 2402 3479 3517 3812 7520 8676 2252
Size: 150 1077 38 295 3708 1156 3039
Fragments arranged by size:
3708 3039 1156 1077 295 150 38
ApoI r'AATT_y
Cuts at: 1493 6772 6998 7150 9259 1493
Size: 5279 226 152 2109 1697
Fragments arranged by size:
5279 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8017 160
Size: 872 6985 1606
Fragments arranged by size:
6985 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9463
AvaI C'yCGr_G
Cuts at: 1870 1943 2086 3977 4067 4288 4600 4772 5041
Size: 73 143 1891 90 221 312 172 269
Cuts at: 5041 5150 5308 5554 6128 6392 6568 6580 6788
Size: 109 158 246 574 264 176 12 208
Cuts at: 6788 1870
Size: 4545
Fragments arranged by size:
4545 1891 574 312 269 264 246 221
208 176 172 158 143 109 90 73
12
BamHI G'GATC_C
Cuts at: 2058 6793 7160 2058
Size: 4735 367 4361
Fragments arranged by size:
4735 4361 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2220 2513 2840 4529 5069
Size: 325 217 627 433 293 327 1689 540
Cuts at: 5069 5246 5401 5459 5847 5869 6283 6784 8239
Size: 177 155 58 388 22 414 501 1455
Cuts at: 8239 618
Size: 1842
Fragments arranged by size:
1842 1689 1455 627 540 501 433 414
388 327 325 293 217 177 155 58
22
BanII G_rGCy'C
Cuts at: 1391 2262 3956 3985 4008 4068 4341 4556 5151
Size: 871 1694 29 23 60 273 215 595
Cuts at: 5151 5360 5456 5638 5732 5771 5791 6066 6135
Size: 209 96 182 94 39 20 275 69
Cuts at: 6135 6322 6596 6713 6782 1391
Size: 187 274 117 69 4072
Fragments arranged by size:
4072 1694 871 595 275 274 273 215
209 187 182 117 96 94 69 69
60 39 29 23 20
BbeI G_GCGC'C
Cuts at: 4533 5073 5405 4533
Size: 540 332 8591
Fragments arranged by size:
8591 540 332
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3336 3572 928
Size: 971 1437 236 6819
Fragments arranged by size:
6819 1437 971 236
BbvCI CC'TCA_GC
Cuts at: 2020 2270 2447 2846 3294 4123 5853 2020
Size: 250 177 399 448 829 1730 5630
Fragments arranged by size:
5630 1730 829 448 399 250 177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2182 2216 3650 3684 7654 7688 1903
Size: 34 245 34 1434 34 3970 34 3678
Fragments arranged by size:
3970 3678 1434 245 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5271 7355 8565 5271
Size: 2084 1210 6169
Fragments arranged by size:
6169 2084 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9463
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4345 4907 5126
Size: 107 122 71 631 605 2673 562 219
Cuts at: 5126 5391 5592 6009 6051 6144 8074 136
Size: 265 201 417 42 93 1930 1525
Fragments arranged by size:
2673 1930 1525 631 605 562 417 265
219 201 122 107 93 71 42
BglII A'GATC_T
Cuts at: 3262 9458 3262
Size: 6196 3267
BlpI GC'TnA_GC
Cuts at: 2803 3026 4400 5765 6880 2803
Size: 223 1374 1365 1115 5386
Fragments arranged by size:
5386 1374 1365 1115 223
Bme1580I G_kGCm'C
Cuts at: 1790 2256 2262 2406 2845 3223 3483 3521 3816
Size: 466 6 144 439 378 260 38 295
Cuts at: 3816 3985 4027 4068 4341 5151 5249 5360 5791
Size: 169 42 41 273 810 98 111 431
Cuts at: 5791 5850 6255 6322 7524 8680 1790
Size: 59 405 67 1202 1156 2573
Fragments arranged by size:
2573 1202 1156 810 466 439 431 405
378 295 273 260 169 144 111 98
67 59 42 41 38 6
BmgBI CAC'GTC
Cuts at: 1318 2647 3787 3811 3931 5200 1318
Size: 1329 1140 24 120 1269 5581
Fragments arranged by size:
5581 1329 1269 1140 120 24
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3334 3979 4817
Size: 416 53 83 399 24 1917 645 838
Cuts at: 4817 5948 7194 8152 442
Size: 1131 1246 958 1753
Fragments arranged by size:
1917 1753 1246 1131 958 838 645 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 5586 1056
Size: 4530 4933
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3550 5470 3550
Size: 1920 7543
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2493 2644 3575 4139 4480 4492 4748
Size: 845 409 151 931 564 341 12 256
Cuts at: 4748 4872 5392 5955 6404 6539 8123 1239
Size: 124 520 563 449 135 1584 2579
Fragments arranged by size:
2579 1584 931 845 564 563 520 449
409 341 256 151 135 124 12
Bpu10I CC'TnA_GC
Cuts at: 2020 2270 2447 2684 2846 3294 4123 5853 2020
Size: 250 177 237 162 448 829 1730 5630
Fragments arranged by size:
5630 1730 829 448 250 237 177 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3239 4184 4199 4208 7588 8453 8751 3239
Size: 945 15 9 3380 865 298 3951
Fragments arranged by size:
3951 3380 945 865 298 15 9
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3305 4074 8126 882
Size: 617 1806 769 4052 2219
Fragments arranged by size:
4052 2219 1806 769 617
BsaAI yAC'GTr
Cuts at: 493 1204 2368 6164 7254 493
Size: 711 1164 3796 1090 2702
Fragments arranged by size:
3796 2702 1164 1090 711
BsaBI GATnn'nnATC
Cuts at: 6561 9457 6561
Size: 2896 6567
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2675 2724 4530
Size: 53 83 186 936 479 663 49 1806
Cuts at: 4530 5070 5402 7270 7652 8287 275
Size: 540 332 1868 382 635 1451
Fragments arranged by size:
1868 1806 1451 936 663 635 540 479
382 332 186 83 53 49
BsaWI w'CCGG_w
Cuts at: 3747 4185 7896 8294 8568 8715 9126 3747
Size: 438 3711 398 274 147 411 4084
Fragments arranged by size:
4084 3711 438 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2604 2634 3401 3431 4538
Size: 30 165 30 1201 30 767 30 1107
Cuts at: 4538 4562 4568 4592 6599 6629 6695 6725 6728
Size: 24 6 24 2007 30 66 30 3
Cuts at: 6728 6758 1178
Size: 30 3883
Fragments arranged by size:
3883 2007 1201 1107 767 165 66 30
30 30 30 30 30 30 24 24
6 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4002 4462 6593 6616 6749 1186
Size: 2816 460 2131 23 133 3900
Fragments arranged by size:
3900 2816 2131 460 133 23
BseYI C'CCAG_C
Cuts at: 2851 2979 3021 3737 5303 5374 5760 5771 6026
Size: 128 42 716 1566 71 386 11 255
Cuts at: 6026 6134 6291 6417 6459 6510 6596 8666 9162
Size: 108 157 126 42 51 86 2070 496
Cuts at: 9162 2851
Size: 3152
Fragments arranged by size:
3152 2070 1566 716 496 386 255 157
128 126 108 86 71 51 42 42
11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3319 3418 3700 3911 4639 4976 5225
Size: 573 1583 99 282 211 728 337 249
Cuts at: 5225 5300 5456 5546 6131 6191 1163
Size: 75 156 90 585 60 4435
Fragments arranged by size:
4435 1583 728 585 573 337 282 249
211 156 99 90 75 60
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3087 3161 4537 5087 6913 7674
Size: 275 186 1086 74 1376 550 1826 761
Cuts at: 7674 8702 1540
Size: 1028 2301
Fragments arranged by size:
2301 1826 1376 1086 1028 761 550 275
186 74
BsiHKAI G_wGCw'C
Cuts at: 2256 2388 2406 2475 3390 3483 3521 3615 3816
Size: 132 18 69 915 93 38 94 201
Cuts at: 3816 3887 3956 4556 5048 5456 6589 6713 7524
Size: 71 69 600 492 408 1133 124 811
Cuts at: 7524 7609 8680 2256
Size: 85 1071 3039
Fragments arranged by size:
3039 1133 1071 915 811 600 492 408
201 132 124 94 93 85 71 69
69 38 18
BsiWI C'GTAC_G
Cuts at: 2364 2364
Size: 9463
BsmI GAATG_Cn'
Cuts at: 6974 7067 6974
Size: 93 9370
BsmBI CGTCTCn'nnnn_
Cuts at: 2003 2003
Size: 9463
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2256 2262 2388 2406 2475 2845 3223
Size: 399 466 6 126 18 69 370 378
Cuts at: 3223 3390 3483 3521 3615 3816 3887 3956 3985
Size: 167 93 38 94 201 71 69 29
Cuts at: 3985 4008 4027 4068 4341 4556 5048 5151 5249
Size: 23 19 41 273 215 492 103 98
Cuts at: 5249 5360 5456 5638 5732 5771 5791 5850 6066
Size: 111 96 182 94 39 20 59 216
Cuts at: 6066 6135 6255 6322 6589 6596 6713 6782 7524
Size: 69 120 67 267 7 117 69 742
Cuts at: 7524 7609 8680 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 492 466 399 378 370
273 267 216 215 201 182 167 126
120 117 111 103 98 96 94 94
93 85 71 69 69 69 69 67
59 41 39 38 29 23 20 19
18 7 6
BspEI T'CCGG_A
Cuts at: 3747 9126 3747
Size: 5379 4084
BspHI T'CATG_A
Cuts at: 7352 9082 7352
Size: 1730 7733
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2589 2745 3582 5245 5625 6302 6818 844
Size: 1745 156 837 1663 380 677 516 3489
Fragments arranged by size:
3489 1745 1663 837 677 516 380 156
BsrBI CCG'CTC
Cuts at: 1394 1823 2595 4470 4506 7350 1394
Size: 429 772 1875 36 2844 3507
Fragments arranged by size:
3507 2844 1875 772 429 36
BsrDI GCAATG_nn'
Cuts at: 65 1248 3811 7958 8132 65
Size: 1183 2563 4147 174 1396
Fragments arranged by size:
4147 2563 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3160 3857 4185 4532 4651 4790 8107
Size: 110 1350 697 328 347 119 139 3317
Cuts at: 8107 8294 1700
Size: 187 2869
Fragments arranged by size:
3317 2869 1350 697 347 328 187 139
119 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9463
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9463
BssSI C'ACGA_G
Cuts at: 2549 3614 4880 7523 8535 2549
Size: 1065 1266 2643 1012 3477
Fragments arranged by size:
3477 2643 1266 1065 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2167 3367 3469 5677 794
Size: 1373 1200 102 2208 4580
Fragments arranged by size:
4580 2208 1373 1200 102
BstBI TT'CG_AA
Cuts at: 8267 8267
Size: 9463
BstEII G'GTnAC_C
Cuts at: 1184 4390 1184
Size: 3206 6257
BstXI CCAn_nnnn'nTGG
Cuts at: 2974 3197 5708 5887 6502 2974
Size: 223 2511 179 615 5935
Fragments arranged by size:
5935 2511 615 223 179
BstYI r'GATC_y
Cuts at: 1972 2058 3262 3705 6354 6793 7160 7545 7562
Size: 86 1204 443 2649 439 367 385 17
Cuts at: 7562 9014 9100 9112 9458 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2649 1977 1452 1204 443 439 385 367
346 86 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3147 5505 3147
Size: 2358 7105
BtgI C'CryG_G
Cuts at: 513 1065 2004 2245 2640 2666 2883 2988 3394
Size: 552 939 241 395 26 217 105 406
Cuts at: 3394 3769 3912 4341 4361 4581 4814 4846 4949
Size: 375 143 429 20 220 233 32 103
Cuts at: 4949 5065 5121 5265 6154 6210 513
Size: 116 56 144 889 56 3766
Fragments arranged by size:
3766 939 889 552 429 406 395 375
241 233 220 217 144 143 116 105
103 56 56 32 26 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5126 6278 487
Size: 683 56 3900 1152 3672
Fragments arranged by size:
3900 3672 1152 683 56
BtsI GCAGTG_nn'
Cuts at: 2025 3082 4027 4330 5444 6979 7773 7793 2025
Size: 1057 945 303 1114 1535 794 20 3695
Fragments arranged by size:
3695 1535 1114 1057 945 794 303 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9463
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4021 4056 529
Size: 35 3457 35 5936
Fragments arranged by size:
5936 3457 35 35
DraI TTT'AAA
Cuts at: 6767 6767
Size: 9463
DraIII CAC_nnn'GTG
Cuts at: 2251 4981 2251
Size: 2730 6733
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8470 809
Size: 507 7154 1802
Fragments arranged by size:
7154 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2808 3084 3158
Size: 54 1425 50 275 18 978 276 74
Cuts at: 3158 4534 4843 4849 5084 5320 5475 5545 5746
Size: 1376 309 6 235 236 155 70 201
Cuts at: 5746 6145 6349 6910 7799 9428 8
Size: 399 204 561 889 1629 43
Fragments arranged by size:
1629 1425 1376 978 889 561 399 309
276 275 236 235 204 201 155 74
70 54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3084 3158 4534 5084 6910 1537
Size: 275 1272 74 1376 550 1826 4090
Fragments arranged by size:
4090 1826 1376 1272 550 275 74
EarI CTCTTCn'nnn_
Cuts at: 1353 7393 1353
Size: 6040 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4911 5015 5353 8050 8424 8570
Size: 1064 138 3322 104 338 2697 374 146
Cuts at: 8570 387
Size: 1280
Fragments arranged by size:
3322 2697 1280 1064 374 338 146 138
104
EcoICRI GAG'CTC
Cuts at: 3954 4554 5454 6711 3954
Size: 600 900 1257 6706
Fragments arranged by size:
6706 1257 900 600
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2493 2644 3575 3584 4139 4300
Size: 393 452 409 151 931 9 555 161
Cuts at: 4300 4480 4492 4748 4872 5392 5955 6404 6539
Size: 180 12 256 124 520 563 449 135
Cuts at: 6539 7526 8123 8910 9178 1239
Size: 987 597 787 268 1524
Fragments arranged by size:
1524 987 931 787 597 563 555 520
452 449 409 393 268 256 180 161
151 135 124 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2508 3233 6190 6731 2508
Size: 725 2957 541 5240
Fragments arranged by size:
5240 2957 725 541
EcoO109I rG'GnC_Cy
Cuts at: 2259 2443 2578 3151 3981 4064 5147 5357 5593
Size: 184 135 573 830 83 1083 210 236
Cuts at: 5593 5787 5788 5914 6022 6318 6319 6523 2259
Size: 194 1 126 108 296 1 204 5199
Fragments arranged by size:
5199 1083 830 573 296 236 210 204
194 184 135 126 108 83 1 1
EcoRI G'AATT_C
Cuts at: 6772 7150 6772
Size: 378 9085
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5557 5557
Size: 9463
FseI GG_CCGG'CC
Cuts at: 3164 3164
Size: 9463
FspI TGC'GCA
Cuts at: 4163 7969 4163
Size: 3806 5657
HaeII r_GCGC'y
Cuts at: 1678 2604 2702 3179 4420 4533 4600 4760 4963
Size: 926 98 477 1241 113 67 160 203
Cuts at: 4963 5073 5235 5405 8610 1678
Size: 110 162 170 3205 2531
Fragments arranged by size:
3205 2531 1241 926 477 203 170 162
160 113 110 98 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3725 3757 4220 4252 4562
Size: 32 135 32 2153 32 463 32 310
Cuts at: 4562 4594 4925 4957 5878 5910 6695 6727 8104
Size: 32 331 32 921 32 785 32 1377
Cuts at: 8104 8136 8178 8210 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2153 1377 921 785 463 331 310
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3103 6484 6807 7053 669
Size: 988 281 1165 3381 323 246 3079
Fragments arranged by size:
3381 3079 1165 988 323 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9463
HpaI GTT'AAC
Cuts at: 7053 7053
Size: 9463
KasI G'GCGC_C
Cuts at: 4529 5069 5401 4529
Size: 540 332 8591
Fragments arranged by size:
8591 540 332
KpnI G_GTAC'C
Cuts at: 2224 6788 2224
Size: 4564 4899
MfeI C'AATT_G
Cuts at: 7062 7062
Size: 9463
MluI A'CGCG_T
Cuts at: 4587 9339 4587
Size: 4752 4711
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3663 3705 4350 5373 8577 8761 1305
Size: 2358 42 645 1023 3204 184 2007
Fragments arranged by size:
3204 2358 2007 1023 645 184 42
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4845 5477 5547 5748 10
Size: 54 1425 343 3013 632 70 201 3725
Fragments arranged by size:
3725 3013 1425 632 343 201 70 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3214 3473 3604 5226 5432 5885 7421
Size: 648 2048 259 131 1622 206 453 1536
Cuts at: 7421 7780 7939 518
Size: 359 159 2042
Fragments arranged by size:
2048 2042 1622 1536 648 453 359 259
206 159 131
MspA1I CmG'CkG
Cuts at: 1606 2072 2123 2135 2274 2456 2668 2743 2807
Size: 466 51 12 139 182 212 75 64
Cuts at: 2807 2814 2837 3244 3364 3423 3829 4145 4159
Size: 7 23 407 120 59 406 316 14
Cuts at: 4159 4343 4363 4408 4559 4623 4717 4816 4951
Size: 184 20 45 151 64 94 99 135
Cuts at: 4951 4996 5067 5631 5677 5764 6181 6879 7556
Size: 45 71 564 46 87 417 698 677
Cuts at: 7556 8704 8949 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 698 677 564 466 417 407
406 316 245 212 184 182 151 139
135 120 99 94 87 75 71 64
64 59 51 46 45 45 23 20
14 12 7
NaeI GCC'GGC
Cuts at: 3162 3859 4534 4653 4792 3162
Size: 697 675 119 139 7833
Fragments arranged by size:
7833 697 675 139 119
NarI GG'CG_CC
Cuts at: 4530 5070 5402 4530
Size: 540 332 8591
Fragments arranged by size:
8591 540 332
NcoI C'CATG_G
Cuts at: 513 1065 2004 2988 6210 513
Size: 552 939 984 3222 3766
Fragments arranged by size:
3766 3222 984 939 552
NdeI CA'TA_TG
Cuts at: 387 3601 387
Size: 3214 6249
NgoMIV G'CCGG_C
Cuts at: 3160 3857 4532 4651 4790 3160
Size: 697 675 119 139 7833
Fragments arranged by size:
7833 697 675 139 119
NheI G'CTAG_C
Cuts at: 1052 5582 1052
Size: 4530 4933
NotI GC'GGCC_GC
Cuts at: 6910 6910
Size: 9463
NspI r_CATG'y
Cuts at: 1132 1229 2203 3472 3487 3758 5431 5671 5824
Size: 97 974 1269 15 271 1673 240 153
Cuts at: 5824 6337 6364 6821 8366 1132
Size: 513 27 457 1545 2229
Fragments arranged by size:
2229 1673 1545 1269 974 513 457 271
240 153 97 27 15
PasI CC'CwG_GG
Cuts at: 1348 2333 4109 4525 5588 5644 5655 1348
Size: 985 1776 416 1063 56 11 5156
Fragments arranged by size:
5156 1776 1063 985 416 56 11
PciI A'CATG_T
Cuts at: 6360 8362 6360
Size: 2002 7461
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3913 5986 6211 6517 1108
Size: 2805 2073 225 306 4054
Fragments arranged by size:
4054 2805 2073 306 225
PfoI T'CCnGG_A
Cuts at: 2061 4305 6516 2061
Size: 2244 2211 5008
Fragments arranged by size:
5008 2244 2211
PmeI GTTT'AAAC
Cuts at: 6767 6767
Size: 9463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6728 6760 7532 7564 9045 9077 6728
Size: 32 772 32 1481 32 7114
Fragments arranged by size:
7114 1481 772 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2578 5914 6022 2578
Size: 3336 108 6019
Fragments arranged by size:
6019 3336 108
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9463
PsiI TTA'TAA
Cuts at: 7033 7033
Size: 9463
PspOMI G'GGCC_C
Cuts at: 2258 3981 4064 4337 5147 5356 5787 6318 2258
Size: 1723 83 273 810 209 431 531 5403
Fragments arranged by size:
5403 1723 810 531 431 273 209 83
PspXI vC'TCGA_Gb
Cuts at: 4600 6128 6568 4600
Size: 1528 440 7495
Fragments arranged by size:
7495 1528 440
PstI C_TGCA'G
Cuts at: 830 2122 2437 2742 3998 4413 5034 5622 5816
Size: 1292 315 305 1256 415 621 588 194
Cuts at: 5816 6815 830
Size: 999 3478
Fragments arranged by size:
3478 1292 1256 999 621 588 415 315
305 194
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9463
PvuII CAG'CTG
Cuts at: 3244 3364 4159 4408 5677 5764 6181 3244
Size: 120 795 249 1269 87 417 6526
Fragments arranged by size:
6526 1269 795 417 249 120 87
RsrII CG'GwC_CG
Cuts at: 3649 3649
Size: 9463
SacI G_AGCT'C
Cuts at: 3956 4556 5456 6713 3956
Size: 600 900 1257 6706
Fragments arranged by size:
6706 1257 900 600
SacII CC_GC'GG
Cuts at: 2669 4344 4364 4817 4952 5068 2669
Size: 1675 20 453 135 116 7064
Fragments arranged by size:
7064 1675 453 135 116 20
SalI G'TCGA_C
Cuts at: 1936 6805 1936
Size: 4869 4594
SanDI GG'GwC_CC
Cuts at: 5914 5914
Size: 9463
SbfI CC_TGCA'GG
Cuts at: 6815 6815
Size: 9463
ScaI AGT'ACT
Cuts at: 7711 9423 7711
Size: 1712 7751
SexAI A'CCwGG_T
Cuts at: 5181 6018 6486 5181
Size: 837 468 8158
Fragments arranged by size:
8158 837 468
SfcI C'TryA_G
Cuts at: 826 1045 2118 2433 2738 3994 4409 5030 5560
Size: 219 1073 315 305 1256 415 621 530
Cuts at: 5560 5576 5618 5812 6090 6811 7946 8627 8818
Size: 16 42 194 278 721 1135 681 191
Cuts at: 8818 826
Size: 1471
Fragments arranged by size:
1471 1256 1135 1073 721 681 621 530
415 315 305 278 219 194 191 42
16
SfoI GGC'GCC
Cuts at: 4531 5071 5403 4531
Size: 540 332 8591
Fragments arranged by size:
8591 540 332
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9463
SgrAI Cr'CCGG_yG
Cuts at: 1700 4651 1700
Size: 2951 6512
SmaI CCC'GGG
Cuts at: 1872 2088 3979 4069 4290 5310 5556 6790 1872
Size: 216 1891 90 221 1020 246 1234 4545
Fragments arranged by size:
4545 1891 1234 1020 246 221 216 90
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2477 3254 4199 4214 4223 4600
Size: 197 926 534 777 945 15 9 377
Cuts at: 4600 6128 6568 7567 8468 8730 820
Size: 1528 440 999 901 262 1553
Fragments arranged by size:
1553 1528 999 945 926 901 777 534
440 377 262 197 15 9
SnaBI TAC'GTA
Cuts at: 493 7254 493
Size: 6761 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9463
SphI G_CATG'C
Cuts at: 1132 6337 6821 1132
Size: 5205 484 3774
Fragments arranged by size:
5205 3774 484
SspI AAT'ATT
Cuts at: 5 52 7387 5
Size: 47 7335 2081
Fragments arranged by size:
7335 2081 47
StuI AGG'CCT
Cuts at: 1517 2099 3431 3542 4517 5022 5574 6381 6437
Size: 582 1332 111 975 505 552 807 56
Cuts at: 6437 1517
Size: 4543
Fragments arranged by size:
4543 1332 975 807 582 552 505 111
56
StyI C'CwwG_G
Cuts at: 513 1065 2004 2146 2988 3339 6210 6255 6902
Size: 552 939 142 842 351 2871 45 647
Cuts at: 6902 513
Size: 3074
Fragments arranged by size:
3074 2871 939 842 647 552 351 142
45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2776 2980 3035 3155 3204 3368 3689
Size: 1255 1196 204 55 120 49 164 321
Cuts at: 3689 3962 4988 5967 7490 7507 7660 7845 8290
Size: 273 1026 979 1523 17 153 185 445
Cuts at: 8290 325
Size: 1498
Fragments arranged by size:
1523 1498 1255 1196 1026 979 445 321
273 204 185 164 153 120 55 49
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3465 4662 5208
Size: 274 80 33 51 1443 1488 1197 546
Cuts at: 5208 7709 9421 96
Size: 2501 1712 138
Fragments arranged by size:
2501 1712 1488 1443 1197 546 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2604 2636 2937 2955 2969 2987 3401 3433 3563
Size: 32 301 18 14 18 414 32 130
Cuts at: 3563 3595 6599 6631 9423 9455 2604
Size: 32 3004 32 2792 32 2612
Fragments arranged by size:
3004 2792 2612 414 301 130 32 32
32 32 32 18 18 14
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9463
XbaI T'CTAG_A
Cuts at: 6799 6799
Size: 9463
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2770 2881 3910 5233 5393 5741 5906 5926 6262
Size: 111 1029 1323 160 348 165 20 336
Cuts at: 6262 2770
Size: 5971
Fragments arranged by size:
5971 1323 1029 348 336 165 160 111
20
XhoI C'TCGA_G
Cuts at: 1943 4600 6128 6568 1943
Size: 2657 1528 440 4838
Fragments arranged by size:
4838 2657 1528 440
XmaI C'CCGG_G
Cuts at: 1870 2086 3977 4067 4288 5308 5554 6788 1870
Size: 216 1891 90 221 1020 246 1234 4545
Fragments arranged by size:
4545 1891 1234 1020 246 221 216 90
XmnI GAAnn'nnTTC
Cuts at: 3452 7592 3452
Size: 4140 5323
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2013 7271 276
Size: 53 83 186 936 479 5258 2468
Fragments arranged by size:
5258 2468 936 479 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BstZ17I EcoRV FspAI NruI
NsiI PacI PmlI PsrI SapI SfiI SrfI SwaI