Restriction map for
pFN21AE0296
(Circular) (Six-base) MAPSORT of: pFN21AE0296.seq Check: 610 from: 1 to: 5668
pFN21AE0296 5668 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3478 278
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3011 1937
Size: 1074 4594
Acc65I G'GTAC_C
Cuts at: 2645 2989 2645
Size: 344 5324
AclI AA'CG_TT
Cuts at: 1563 3795 4168 1563
Size: 2232 373 3063
Fragments arranged by size:
3063 2232 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2515 2846 3731 5115 5383 1632
Size: 883 331 885 1384 268 1917
Fragments arranged by size:
1917 1384 885 883 331 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5471
AflIII A'CryG_T
Cuts at: 1203 4567 5544 1203
Size: 3364 977 1327
Fragments arranged by size:
3364 1327 977
AgeI A'CCGG_T
Cuts at: 4499 4499
Size: 5668
AhdI GACnn_n'nnGTC
Cuts at: 2734 2896 4397 2734
Size: 162 1501 4005
Fragments arranged by size:
4005 1501 162
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5668
AlwNI CAG_nnn'CTG
Cuts at: 2074 2341 3411 4983 2074
Size: 267 1070 1572 2759
Fragments arranged by size:
2759 1572 1070 267
ApaI G_GGCC'C
Cuts at: 2280 2439 2280
Size: 159 5509
ApaLI G'TGCA_C
Cuts at: 3725 4881 3725
Size: 1156 4512
ApoI r'AATT_y
Cuts at: 1493 2977 3203 3355 5464 1493
Size: 1484 226 152 2109 1697
Fragments arranged by size:
2109 1697 1484 226 152
AscI GG'CGCG_CC
Cuts at: 2148 2148
Size: 5668
AseI AT'TA_AT
Cuts at: 160 1032 4222 160
Size: 872 3190 1606
Fragments arranged by size:
3190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
AvaI C'yCGr_G
Cuts at: 1870 1943 2153 2438 2759 2993 1870
Size: 73 210 285 321 234 4545
Fragments arranged by size:
4545 321 285 234 210 73
AvrII C'CTAG_G
Cuts at: 2806 2806
Size: 5668
BamHI G'GATC_C
Cuts at: 2998 3365 2998
Size: 367 5301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2250 2282 2348 2594 2645
Size: 325 217 627 463 32 66 246 51
Cuts at: 2645 2810 2989 4444 618
Size: 165 179 1455 1842
Fragments arranged by size:
1842 1455 627 463 325 246 217 179
165 66 51 32
BanII G_rGCy'C
Cuts at: 1391 2179 2280 2439 2493 2987 1391
Size: 788 101 159 54 494 4072
Fragments arranged by size:
4072 788 494 159 101 54
BbeI G_GCGC'C
Cuts at: 2254 2286 2352 2814 2254
Size: 32 66 462 5108
Fragments arranged by size:
5108 462 66 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2290 2834 928
Size: 971 391 544 3762
Fragments arranged by size:
3762 971 544 391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3859 3893 1903
Size: 34 1922 34 3678
Fragments arranged by size:
3678 1922 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3560 4770 3560
Size: 1210 4458
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5668
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2063 2165
Size: 107 122 71 631 605 337 54 102
Cuts at: 2165 2346 4279 136
Size: 181 1933 1525
Fragments arranged by size:
1933 1525 631 605 337 181 122 107
102 71 54
BglII A'GATC_T
Cuts at: 5663 5663
Size: 5668
BlpI GC'TnA_GC
Cuts at: 2051 3085 2051
Size: 1034 4634
Bme1580I G_kGCm'C
Cuts at: 1790 2068 2280 2439 2597 3729 4885 1790
Size: 278 212 159 158 1132 1156 2573
Fragments arranged by size:
2573 1156 1132 278 212 159 158
BmgBI CAC'GTC
Cuts at: 1318 2086 2497 1318
Size: 768 411 4489
Fragments arranged by size:
4489 768 411
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3399 4357 442
Size: 416 53 83 399 24 1982 958 1753
Fragments arranged by size:
1982 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2312 2423 2507 4328 1239
Size: 1073 111 84 1821 2579
Fragments arranged by size:
2579 1821 1073 111 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2642 3793 4658 4956 2642
Size: 1151 865 298 3354
Fragments arranged by size:
3354 1151 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2126 2775 4331 882
Size: 617 627 649 1556 2219
Fragments arranged by size:
2219 1556 649 627 617
BsaAI yAC'GTr
Cuts at: 493 1204 2371 3459 493
Size: 711 1167 1088 2702
Fragments arranged by size:
2702 1167 1088 711
BsaBI GATnn'nnATC
Cuts at: 5662 5662
Size: 5668
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2034 2251 2283 2349
Size: 53 83 186 936 501 217 32 66
Cuts at: 2349 2415 2811 3475 3857 4492 275
Size: 66 396 664 382 635 1451
Fragments arranged by size:
1451 936 664 635 501 396 382 217
186 83 66 66 53 32
BsaWI w'CCGG_w
Cuts at: 2846 4101 4499 4773 4920 5331 2846
Size: 1255 398 274 147 411 3183
Fragments arranged by size:
3183 1255 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5443
Fragments arranged by size:
5443 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5668
BseYI C'CCAG_C
Cuts at: 2374 2383 2682 2711 4871 5367 2374
Size: 9 299 29 2160 496 2675
Fragments arranged by size:
2675 2160 496 299 29 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2299 2459 1163
Size: 573 563 160 4372
Fragments arranged by size:
4372 573 563 160
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2632 3118 3879 4907 1540
Size: 275 186 631 486 761 1028 2301
Fragments arranged by size:
2301 1028 761 631 486 275 186
BsiHKAI G_wGCw'C
Cuts at: 3729 3814 4885 3729
Size: 85 1071 4512
Fragments arranged by size:
4512 1071 85
BsmI GAATG_Cn'
Cuts at: 3179 3272 3179
Size: 93 5575
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2179 2280 2439 2493 2597 2987
Size: 399 278 111 101 159 54 104 390
Cuts at: 2987 3729 3814 4885 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 390 278 159 111
104 101 85 54
BspEI T'CCGG_A
Cuts at: 5331 5331
Size: 5668
BspHI T'CATG_A
Cuts at: 3557 5287 3557
Size: 1730 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2337 2574 3023 844
Size: 1493 237 449 3489
Fragments arranged by size:
3489 1493 449 237
BsrBI CCG'CTC
Cuts at: 1394 1823 2062 3555 1394
Size: 429 239 1493 3507
Fragments arranged by size:
3507 1493 429 239
BsrDI GCAATG_nn'
Cuts at: 65 1248 4163 4337 65
Size: 1183 2915 174 1396
Fragments arranged by size:
2915 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2021 2345 2610 4312 4499 1700
Size: 110 211 324 265 1702 187 2869
Fragments arranged by size:
2869 1702 324 265 211 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5668
BssHII G'CGCG_C
Cuts at: 1925 2148 2500 1925
Size: 223 352 5093
Fragments arranged by size:
5093 352 223
BssSI C'ACGA_G
Cuts at: 2588 3728 4740 2588
Size: 1140 1012 3516
Fragments arranged by size:
3516 1140 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5668
BstBI TT'CG_AA
Cuts at: 4472 4472
Size: 5668
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5668
BstYI r'GATC_y
Cuts at: 1972 2998 3365 3750 3767 5219 5305 5317 5663
Size: 1026 367 385 17 1452 86 12 346
Cuts at: 5663 1972
Size: 1977
Fragments arranged by size:
1977 1452 1026 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2179 2271 2394 2409 2667 513
Size: 552 939 175 92 123 15 258 3514
Fragments arranged by size:
3514 939 552 258 175 123 92 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2623 487
Size: 683 56 1397 3532
Fragments arranged by size:
3532 1397 683 56
BtsI GCAGTG_nn'
Cuts at: 2444 3184 3978 3998 2444
Size: 740 794 20 4114
Fragments arranged by size:
4114 794 740 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5633
DraI TTT'AAA
Cuts at: 2972 2972
Size: 5668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4675 809
Size: 507 3359 1802
Fragments arranged by size:
3359 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2343 2391
Size: 54 1425 50 275 18 249 264 48
Cuts at: 2391 2629 2676 3115 4004 5633 8
Size: 238 47 439 889 1629 43
Fragments arranged by size:
1629 1425 889 439 275 264 249 238
54 50 48 47 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2629 3115 1537
Size: 275 817 486 4090
Fragments arranged by size:
4090 817 486 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3598 1353
Size: 2245 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2030 4255 4629 4775 387
Size: 1064 138 441 2225 374 146 1280
Fragments arranged by size:
2225 1280 1064 441 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 2423 2507 2515 2846 3731 4328
Size: 393 680 111 84 8 331 885 597
Cuts at: 4328 5115 5383 1239
Size: 787 268 1524
Fragments arranged by size:
1524 885 787 680 597 393 331 268
111 84 8
EcoO109I rG'GnC_Cy
Cuts at: 2158 2276 2435 2459 2554 2158
Size: 118 159 24 95 5272
Fragments arranged by size:
5272 159 118 95 24
EcoRI G'AATT_C
Cuts at: 2977 3355 2977
Size: 378 5290
FspI TGC'GCA
Cuts at: 4174 4174
Size: 5668
HaeII r_GCGC'y
Cuts at: 1678 2058 2075 2171 2191 2254 2286 2341 2352
Size: 380 17 96 20 63 32 55 11
Cuts at: 2352 2689 2814 4815 1678
Size: 337 125 2001 2531
Fragments arranged by size:
2531 2001 380 337 125 96 63 55
32 20 17 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2720 2752 2882 2914 4309
Size: 32 135 32 1148 32 130 32 1395
Cuts at: 4309 4341 4383 4415 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1395 1148 135 130 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2932 3012 3258 669
Size: 988 281 994 80 246 3079
Fragments arranged by size:
3079 994 988 281 246 80
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5668
HpaI GTT'AAC
Cuts at: 3258 3258
Size: 5668
KasI G'GCGC_C
Cuts at: 2250 2282 2348 2810 2250
Size: 32 66 462 5108
Fragments arranged by size:
5108 462 66 32
KpnI G_GTAC'C
Cuts at: 2649 2993 2649
Size: 344 5324
MfeI C'AATT_G
Cuts at: 3267 3267
Size: 5668
MluI A'CGCG_T
Cuts at: 5544 5544
Size: 5668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4782 4966 1305
Size: 3477 184 2007
Fragments arranged by size:
3477 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3846
Fragments arranged by size:
3846 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2600 2771 3626 3985 4144 518
Size: 648 1434 171 855 359 159 2042
Fragments arranged by size:
2042 1434 855 648 359 171 159
MspA1I CmG'CkG
Cuts at: 1606 2040 2181 2273 2396 2485 3084 3761 4909
Size: 434 141 92 123 89 599 677 1148
Cuts at: 4909 5154 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 599 434 245 141 123
92 89
NaeI GCC'GGC
Cuts at: 2347 2347
Size: 5668
NarI GG'CG_CC
Cuts at: 2251 2283 2349 2811 2251
Size: 32 66 462 5108
Fragments arranged by size:
5108 462 66 32
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4177
Fragments arranged by size:
4177 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5668
NgoMIV G'CCGG_C
Cuts at: 2345 2345
Size: 5668
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5668
NotI GC'GGCC_GC
Cuts at: 3115 3115
Size: 5668
NspI r_CATG'y
Cuts at: 1132 1229 3026 4571 1132
Size: 97 1797 1545 2229
Fragments arranged by size:
2229 1797 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5668
PciI A'CATG_T
Cuts at: 4567 4567
Size: 5668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2341 1108
Size: 1233 4435
PmeI GTTT'AAAC
Cuts at: 2972 2972
Size: 5668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 5250 5282 3737
Size: 32 1481 32 4123
Fragments arranged by size:
4123 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2459 2459
Size: 5668
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5668
PsiI TTA'TAA
Cuts at: 3238 3238
Size: 5668
PspOMI G'GGCC_C
Cuts at: 2276 2435 2276
Size: 159 5509
PstI C_TGCA'G
Cuts at: 830 2451 2571 3020 830
Size: 1621 120 449 3478
Fragments arranged by size:
3478 1621 449 120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5668
SacII CC_GC'GG
Cuts at: 2182 2274 2397 2182
Size: 92 123 5453
Fragments arranged by size:
5453 123 92
SalI G'TCGA_C
Cuts at: 1936 3010 1936
Size: 1074 4594
SanDI GG'GwC_CC
Cuts at: 2459 2459
Size: 5668
SbfI CC_TGCA'GG
Cuts at: 3020 3020
Size: 5668
ScaI AGT'ACT
Cuts at: 3916 5628 3916
Size: 1712 3956
SfcI C'TryA_G
Cuts at: 826 1045 2447 2567 3016 4151 4832 5023 826
Size: 219 1402 120 449 1135 681 191 1471
Fragments arranged by size:
1471 1402 1135 681 449 219 191 120
SfoI GGC'GCC
Cuts at: 2252 2284 2350 2812 2252
Size: 32 66 462 5108
Fragments arranged by size:
5108 462 66 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5668
SmaI CCC'GGG
Cuts at: 1872 2995 1872
Size: 1123 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2621 3772 4673 4935 820
Size: 197 926 678 1151 901 262 1553
Fragments arranged by size:
1553 1151 926 901 678 262 197
SnaBI TAC'GTA
Cuts at: 493 3459 493
Size: 2966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5668
SphI G_CATG'C
Cuts at: 1132 3026 1132
Size: 1894 3774
SspI AAT'ATT
Cuts at: 5 52 3592 5
Size: 47 3540 2081
Fragments arranged by size:
3540 2081 47
StuI AGG'CCT
Cuts at: 1517 2366 1517
Size: 849 4819
StyI C'CwwG_G
Cuts at: 513 1065 2004 2361 2550 2806 3107 513
Size: 552 939 357 189 256 301 3074
Fragments arranged by size:
3074 939 552 357 301 256 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3695 3712 3865 4050 4495 325
Size: 1255 2115 17 153 185 445 1498
Fragments arranged by size:
2115 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3914 5626 96
Size: 274 80 33 51 1443 1937 1712 138
Fragments arranged by size:
1937 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5628 5660 5628
Size: 32 5636
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5668
XbaI T'CTAG_A
Cuts at: 3004 3004
Size: 5668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2428 2428
Size: 5668
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5668
XmaI C'CCGG_G
Cuts at: 1870 2993 1870
Size: 1123 4545
XmnI GAAnn'nnTTC
Cuts at: 3797 3797
Size: 5668
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3476 276
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SapI SexAI
SfiI SrfI SwaI