Restriction map for
pFN21AE0295
(Circular) (Six-base) MAPSORT of: pFN21AE0295.seq Check: 7322 from: 1 to: 5413
pFN21AE0295 5413 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3223 278
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2756 1937
Size: 819 4594
Acc65I G'GTAC_C
Cuts at: 2734 2734
Size: 5413
AclI AA'CG_TT
Cuts at: 1563 3540 3913 1563
Size: 1977 373 3063
Fragments arranged by size:
3063 1977 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3476 4860 5128 1632
Size: 1844 1384 268 1917
Fragments arranged by size:
1917 1844 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5216
AflIII A'CryG_T
Cuts at: 1203 4312 5289 1203
Size: 3109 977 1327
Fragments arranged by size:
3109 1327 977
AgeI A'CCGG_T
Cuts at: 4244 4244
Size: 5413
AhdI GACnn_n'nnGTC
Cuts at: 4142 4142
Size: 5413
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5413
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5413
AlwNI CAG_nnn'CTG
Cuts at: 3156 4728 3156
Size: 1572 3841
ApaI G_GGCC'C
Cuts at: 2337 2497 2665 2337
Size: 160 168 5085
Fragments arranged by size:
5085 168 160
ApaLI G'TGCA_C
Cuts at: 2585 3470 4626 2585
Size: 885 1156 3372
Fragments arranged by size:
3372 1156 885
ApoI r'AATT_y
Cuts at: 1493 2722 2948 3100 5209 1493
Size: 1229 226 152 2109 1697
Fragments arranged by size:
2109 1697 1229 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3967 160
Size: 872 2935 1606
Fragments arranged by size:
2935 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
AvaI C'yCGr_G
Cuts at: 1870 1943 2019 2078 2166 2421 2490 2496 2738
Size: 73 76 59 88 255 69 6 242
Cuts at: 2738 1870
Size: 4545
Fragments arranged by size:
4545 255 242 88 76 73 69 59
6
BamHI G'GATC_C
Cuts at: 2743 3110 2743
Size: 367 5046
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 2150 2288 2400 2408
Size: 325 217 627 227 136 138 112 8
Cuts at: 2408 2486 2734 4189 618
Size: 78 248 1455 1842
Fragments arranged by size:
1842 1455 627 325 248 227 217 138
136 112 78 8
BanII G_rGCy'C
Cuts at: 1391 2064 2079 2337 2497 2665 2676 2732 1391
Size: 673 15 258 160 168 11 56 4072
Fragments arranged by size:
4072 673 258 168 160 56 15 11
BbeI G_GCGC'C
Cuts at: 2292 2404 2292
Size: 112 5301
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2029 928
Size: 971 130 4312
Fragments arranged by size:
4312 971 130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2132 2166 3604 3638 1903
Size: 34 195 34 1438 34 3678
Fragments arranged by size:
3678 1438 195 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3305 4515 3305
Size: 1210 4203
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5413
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4024 136
Size: 107 122 71 631 605 2352 1525
Fragments arranged by size:
2352 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5408 5408
Size: 5413
BlpI GC'TnA_GC
Cuts at: 2830 2830
Size: 5413
Bme1580I G_kGCm'C
Cuts at: 1790 2155 2201 2337 2413 2497 2589 2665 3474
Size: 365 46 136 76 84 92 76 809
Cuts at: 3474 4630 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 809 365 136 92 84 76
76 46
BmgBI CAC'GTC
Cuts at: 1318 2584 1318
Size: 1266 4147
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2061 2067 3144
Size: 416 53 83 399 24 644 6 1077
Cuts at: 3144 4102 442
Size: 958 1753
Fragments arranged by size:
1753 1077 958 644 416 399 83 53
24 6
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 2726 4073 1239
Size: 905 582 1347 2579
Fragments arranged by size:
2579 1347 905 582
Bpu10I CC'TnA_GC
Cuts at: 2243 2318 2243
Size: 75 5338
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3538 4403 4701 3538
Size: 865 298 4250
Fragments arranged by size:
4250 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2021 4076 882
Size: 617 522 2055 2219
Fragments arranged by size:
2219 2055 617 522
BsaAI yAC'GTr
Cuts at: 493 1204 3204 493
Size: 711 2000 2702
Fragments arranged by size:
2702 2000 711
BsaBI GATnn'nnATC
Cuts at: 5407 5407
Size: 5413
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2289 2401 3220 3602
Size: 53 83 186 936 756 112 819 382
Cuts at: 3602 4237 275
Size: 635 1451
Fragments arranged by size:
1451 936 819 756 635 382 186 112
83 53
BsaWI w'CCGG_w
Cuts at: 3846 4244 4518 4665 5076 3846
Size: 398 274 147 411 4183
Fragments arranged by size:
4183 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2102 2132 1178
Size: 30 165 30 699 30 4459
Fragments arranged by size:
4459 699 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2107 1186
Size: 921 4492
BseYI C'CCAG_C
Cuts at: 2699 4616 5112 2699
Size: 1917 496 3000
Fragments arranged by size:
3000 1917 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2594 1163
Size: 573 858 3982
Fragments arranged by size:
3982 858 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2863 3624 4652 1540
Size: 275 186 862 761 1028 2301
Fragments arranged by size:
2301 1028 862 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2139 2589 3474 3559 4630 2139
Size: 450 885 85 1071 2922
Fragments arranged by size:
2922 1071 885 450 85
BsmI GAATG_Cn'
Cuts at: 2924 3017 2924
Size: 93 5320
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2079 2139 2155 2201 2337 2413
Size: 399 274 15 60 16 46 136 76
Cuts at: 2413 2497 2589 2665 2676 2732 3474 3559 4630
Size: 84 92 76 11 56 742 85 1071
Cuts at: 4630 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 274 136 92 85
84 76 76 60 56 46 16 15
11
BspEI T'CCGG_A
Cuts at: 5076 5076
Size: 5413
BspHI T'CATG_A
Cuts at: 3302 5032 3302
Size: 1730 3683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2768 844
Size: 1924 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2115 3300 1394
Size: 429 292 1185 3507
Fragments arranged by size:
3507 1185 429 292
BsrDI GCAATG_nn'
Cuts at: 65 1248 3908 4082 65
Size: 1183 2660 174 1396
Fragments arranged by size:
2660 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2237 2449 4057 4244 1700
Size: 110 427 212 1608 187 2869
Fragments arranged by size:
2869 1608 427 212 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5413
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5413
BssSI C'ACGA_G
Cuts at: 3473 4485 3473
Size: 1012 4401
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2593 794
Size: 1799 3614
BstBI TT'CG_AA
Cuts at: 4217 4217
Size: 5413
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5413
BstYI r'GATC_y
Cuts at: 1972 2743 3110 3495 3512 4964 5050 5062 5408
Size: 771 367 385 17 1452 86 12 346
Cuts at: 5408 1972
Size: 1977
Fragments arranged by size:
1977 1452 771 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2292 2482 2653 513
Size: 552 1227 190 171 3273
Fragments arranged by size:
3273 1227 552 190 171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2278 487
Size: 683 56 1052 3622
Fragments arranged by size:
3622 1052 683 56
BtsI GCAGTG_nn'
Cuts at: 2214 2254 2929 3723 3743 2214
Size: 40 675 794 20 3884
Fragments arranged by size:
3884 794 675 40 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5413
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5378
DraI TTT'AAA
Cuts at: 2717 2717
Size: 5413
DraIII CAC_nnn'GTG
Cuts at: 2190 2190
Size: 5413
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4420 809
Size: 507 3104 1802
Fragments arranged by size:
3104 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2860 3749 5378
Size: 54 1425 50 275 18 1030 889 1629
Cuts at: 5378 8
Size: 43
Fragments arranged by size:
1629 1425 1030 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2860 1537
Size: 275 1048 4090
Fragments arranged by size:
4090 1048 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2534 3343 1353
Size: 1181 809 3423
Fragments arranged by size:
3423 1181 809
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4000 4374 4520 387
Size: 1064 138 2411 374 146 1280
Fragments arranged by size:
2411 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2726 3476 4073 4860 5128 1239
Size: 393 512 582 750 597 787 268 1524
Fragments arranged by size:
1524 787 750 597 582 512 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2170 2254 2333 2461 2170
Size: 84 79 128 5122
Fragments arranged by size:
5122 128 84 79
EcoRI G'AATT_C
Cuts at: 2722 3100 2722
Size: 378 5035
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2296 2296
Size: 5413
FspI TGC'GCA
Cuts at: 3919 3919
Size: 5413
HaeII r_GCGC'y
Cuts at: 1678 2209 2292 2404 4560 1678
Size: 531 83 112 2156 2531
Fragments arranged by size:
2531 2156 531 112 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4054 4086 4128 4160 1373
Size: 32 135 32 2482 32 42 32 2626
Fragments arranged by size:
2626 2482 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2757 3003 669
Size: 988 281 819 246 3079
Fragments arranged by size:
3079 988 819 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5413
HpaI GTT'AAC
Cuts at: 3003 3003
Size: 5413
KasI G'GCGC_C
Cuts at: 2288 2400 2288
Size: 112 5301
KpnI G_GTAC'C
Cuts at: 2738 2738
Size: 5413
MfeI C'AATT_G
Cuts at: 3012 3012
Size: 5413
MluI A'CGCG_T
Cuts at: 5289 5289
Size: 5413
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4527 4711 1305
Size: 3222 184 2007
Fragments arranged by size:
3222 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3591
Fragments arranged by size:
3591 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3371 3730 3889 518
Size: 648 2205 359 159 2042
Fragments arranged by size:
2205 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2829 3506 4654 4899 1606
Size: 1223 677 1148 245 2120
Fragments arranged by size:
2120 1223 1148 677 245
NaeI GCC'GGC
Cuts at: 2451 2451
Size: 5413
NarI GG'CG_CC
Cuts at: 2289 2401 2289
Size: 112 5301
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4861
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5413
NgoMIV G'CCGG_C
Cuts at: 2449 2449
Size: 5413
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5413
NotI GC'GGCC_GC
Cuts at: 2860 2860
Size: 5413
NspI r_CATG'y
Cuts at: 1132 1229 2197 2771 4316 1132
Size: 97 968 574 1545 2229
Fragments arranged by size:
2229 1545 968 574 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5413
PciI A'CATG_T
Cuts at: 4312 4312
Size: 5413
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5413
PfoI T'CCnGG_A
Cuts at: 2040 2172 2040
Size: 132 5281
PmeI GTTT'AAAC
Cuts at: 2717 2717
Size: 5413
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 4995 5027 3482
Size: 32 1481 32 3868
Fragments arranged by size:
3868 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2170 2170
Size: 5413
PshAI GACnn'nnGTC
Cuts at: 1613 2033 1613
Size: 420 4993
PsiI TTA'TAA
Cuts at: 2983 2983
Size: 5413
PspOMI G'GGCC_C
Cuts at: 2333 2493 2661 2333
Size: 160 168 5085
Fragments arranged by size:
5085 168 160
PstI C_TGCA'G
Cuts at: 830 2310 2765 830
Size: 1480 455 3478
Fragments arranged by size:
3478 1480 455
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5413
SalI G'TCGA_C
Cuts at: 1936 2755 1936
Size: 819 4594
SbfI CC_TGCA'GG
Cuts at: 2765 2765
Size: 5413
ScaI AGT'ACT
Cuts at: 3661 5373 3661
Size: 1712 3701
SfcI C'TryA_G
Cuts at: 826 1045 2306 2761 3896 4577 4768 826
Size: 219 1261 455 1135 681 191 1471
Fragments arranged by size:
1471 1261 1135 681 455 219 191
SfoI GGC'GCC
Cuts at: 2290 2402 2290
Size: 112 5301
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5413
SmaI CCC'GGG
Cuts at: 1872 2021 2168 2423 2492 2498 2740 1872
Size: 149 147 255 69 6 242 4545
Fragments arranged by size:
4545 255 242 149 147 69 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3517 4418 4680 820
Size: 197 926 1574 901 262 1553
Fragments arranged by size:
1574 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3204 493
Size: 2711 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5413
SphI G_CATG'C
Cuts at: 1132 2771 1132
Size: 1639 3774
SspI AAT'ATT
Cuts at: 5 52 3337 5
Size: 47 3285 2081
Fragments arranged by size:
3285 2081 47
StuI AGG'CCT
Cuts at: 1517 2312 1517
Size: 795 4618
StyI C'CwwG_G
Cuts at: 513 1065 2220 2852 513
Size: 552 1155 632 3074
Fragments arranged by size:
3074 1155 632 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2396 2646 3440 3457 3610 3795 4240
Size: 1255 816 250 794 17 153 185 445
Cuts at: 4240 325
Size: 1498
Fragments arranged by size:
1498 1255 816 794 445 250 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2679 3659 5371
Size: 274 80 33 51 1443 702 980 1712
Cuts at: 5371 96
Size: 138
Fragments arranged by size:
1712 1443 980 702 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2102 2134 5373 5405 2102
Size: 32 3239 32 2110
Fragments arranged by size:
3239 2110 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2429 1316
Size: 1113 4300
XbaI T'CTAG_A
Cuts at: 2749 2749
Size: 5413
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2344 2344
Size: 5413
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5413
XmaI C'CCGG_G
Cuts at: 1870 2019 2166 2421 2490 2496 2738 1870
Size: 149 147 255 69 6 242 4545
Fragments arranged by size:
4545 255 242 149 147 69 6
XmnI GAAnn'nnTTC
Cuts at: 3542 3542
Size: 5413
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3221 276
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI