Restriction map for
pFN21AE0294
(Circular) (Six-base) MAPSORT of: pFN21AE0294.seq Check: 5959 from: 1 to: 5647
pFN21AE0294 5647 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2268 2268
Size: 5647
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2871 2990 1937
Size: 934 119 4594
Fragments arranged by size:
4594 934 119
Acc65I G'GTAC_C
Cuts at: 2968 2968
Size: 5647
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2143 2705 3710 5094 5362 1632
Size: 511 562 1005 1384 268 1917
Fragments arranged by size:
1917 1384 1005 562 511 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 4546 5523 1203
Size: 3343 977 1327
Fragments arranged by size:
3343 1327 977
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 4376 4376
Size: 5647
AleI CACnn'nnGTG
Cuts at: 1166 2585 1166
Size: 1419 4228
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5647
AlwNI CAG_nnn'CTG
Cuts at: 2083 2251 3390 4962 2083
Size: 168 1139 1572 2768
Fragments arranged by size:
2768 1572 1139 168
ApaLI G'TGCA_C
Cuts at: 3704 4860 3704
Size: 1156 4491
ApoI r'AATT_y
Cuts at: 1493 2956 3182 3334 5443 1493
Size: 1463 226 152 2109 1697
Fragments arranged by size:
2109 1697 1463 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4201 160
Size: 872 3169 1606
Fragments arranged by size:
3169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2972 1870
Size: 73 1029 4545
Fragments arranged by size:
4545 1029 73
BamHI G'GATC_C
Cuts at: 2977 3344 2977
Size: 367 5280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2551 2632 2968 4423 618
Size: 325 217 627 764 81 336 1455 1842
Fragments arranged by size:
1842 1455 764 627 336 325 217 81
BanII G_rGCy'C
Cuts at: 1391 2076 2966 1391
Size: 685 890 4072
Fragments arranged by size:
4072 890 685
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4676
BbvCI CC'TCA_GC
Cuts at: 2244 2612 2695 2700 2244
Size: 368 83 5 5191
Fragments arranged by size:
5191 368 83 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3838 3872 1903
Size: 34 50 34 1817 34 3678
Fragments arranged by size:
3678 1817 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3539 4749 3539
Size: 1210 4437
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4258 136
Size: 107 122 71 631 605 2586 1525
Fragments arranged by size:
2586 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5642 5642
Size: 5647
BlpI GC'TnA_GC
Cuts at: 3064 3064
Size: 5647
Bme1580I G_kGCm'C
Cuts at: 1790 3708 4864 1790
Size: 1918 1156 2573
Fragments arranged by size:
2573 1918 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5647
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3378 4336 442
Size: 416 53 83 399 24 1961 958 1753
Fragments arranged by size:
1961 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2044 2060 4307 1239
Size: 805 16 2247 2579
Fragments arranged by size:
2579 2247 805 16
Bpu10I CC'TnA_GC
Cuts at: 2244 2612 2695 2700 2244
Size: 368 83 5 5191
Fragments arranged by size:
5191 368 83 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3772 4637 4935 3772
Size: 865 298 4484
Fragments arranged by size:
4484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4310 882
Size: 617 2811 2219
Fragments arranged by size:
2811 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3438 493
Size: 711 2234 2702
Fragments arranged by size:
2702 2234 711
BsaBI GATnn'nnATC
Cuts at: 5641 5641
Size: 5647
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3454 3836 4471 275
Size: 53 83 186 936 1921 382 635 1451
Fragments arranged by size:
1921 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4080 4478 4752 4899 5310 4080
Size: 398 274 147 411 4417
Fragments arranged by size:
4417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5422
Fragments arranged by size:
5422 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2336 1186
Size: 1150 4497
BseYI C'CCAG_C
Cuts at: 2304 2425 4850 5346 2304
Size: 121 2425 496 2605
Fragments arranged by size:
2605 2425 496 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2557 1163
Size: 573 821 4253
Fragments arranged by size:
4253 821 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3097 3858 4886 1540
Size: 275 186 1096 761 1028 2301
Fragments arranged by size:
2301 1096 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 3708 3793 4864 2076
Size: 1632 85 1071 2859
Fragments arranged by size:
2859 1632 1071 85
BsmI GAATG_Cn'
Cuts at: 2928 3158 3251 2928
Size: 230 93 5324
Fragments arranged by size:
5324 230 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2966 3708 3793 4864 1391
Size: 399 286 890 742 85 1071 2174
Fragments arranged by size:
2174 1071 890 742 399 286 85
BspEI T'CCGG_A
Cuts at: 5310 5310
Size: 5647
BspHI T'CATG_A
Cuts at: 3536 5266 3536
Size: 1730 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2268 3002 844
Size: 1424 734 3489
Fragments arranged by size:
3489 1424 734
BsrBI CCG'CTC
Cuts at: 1394 1823 3534 1394
Size: 429 1711 3507
Fragments arranged by size:
3507 1711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2349 4142 4316 65
Size: 1183 1101 1793 174 1396
Fragments arranged by size:
1793 1396 1183 1101 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4291 4478 1700
Size: 110 2481 187 2869
Fragments arranged by size:
2869 2481 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5647
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 3707 4719 3707
Size: 1012 4635
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2340 794
Size: 1546 4101
BstBI TT'CG_AA
Cuts at: 4451 4451
Size: 5647
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5647
BstYI r'GATC_y
Cuts at: 1972 2688 2977 3344 3729 3746 5198 5284 5296
Size: 716 289 367 385 17 1452 86 12
Cuts at: 5296 5642 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 716 385 367 346 289 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5095
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4908
Fragments arranged by size:
4908 683 56
BtsI GCAGTG_nn'
Cuts at: 2835 3163 3957 3977 2835
Size: 328 794 20 4505
Fragments arranged by size:
4505 794 328 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2106 2141 529
Size: 35 1542 35 4035
Fragments arranged by size:
4035 1542 35 35
DraI TTT'AAA
Cuts at: 2951 2951
Size: 5647
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4654 809
Size: 507 3338 1802
Fragments arranged by size:
3338 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2145 2481
Size: 54 1425 50 275 18 297 18 336
Cuts at: 2481 3094 3983 5612 8
Size: 613 889 1629 43
Fragments arranged by size:
1629 1425 889 613 336 297 275 54
50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3094 1537
Size: 275 1282 4090
Fragments arranged by size:
4090 1282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3577 1353
Size: 2224 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4234 4608 4754 387
Size: 1064 138 2645 374 146 1280
Fragments arranged by size:
2645 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2074 2074
Size: 5647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2044 2060 2143 2705 3710 4307 5094
Size: 393 412 16 83 562 1005 597 787
Cuts at: 5094 5362 1239
Size: 268 1524
Fragments arranged by size:
1524 1005 787 597 562 412 393 268
83 16
EcoRI G'AATT_C
Cuts at: 2956 3334 2956
Size: 378 5269
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2731 2731
Size: 5647
FspI TGC'GCA
Cuts at: 4153 4153
Size: 5647
HaeII r_GCGC'y
Cuts at: 1678 4794 1678
Size: 3116 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4288 4320 4362 4394 1373
Size: 32 135 32 2716 32 42 32 2626
Fragments arranged by size:
2716 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2530 2674 2991 3237 669
Size: 988 281 592 144 317 246 3079
Fragments arranged by size:
3079 988 592 317 281 246 144
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5647
HpaI GTT'AAC
Cuts at: 2530 2674 3237 2530
Size: 144 563 4940
Fragments arranged by size:
4940 563 144
KpnI G_GTAC'C
Cuts at: 2972 2972
Size: 5647
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 5523 5523
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2412 4761 4945 1305
Size: 1107 2349 184 2007
Fragments arranged by size:
2349 2007 1107 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2129 2483 10
Size: 54 1425 343 297 354 3174
Fragments arranged by size:
3174 1425 354 343 297 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2048 2180 2354 2585 3605 3964 4123
Size: 648 882 132 174 231 1020 359 159
Cuts at: 4123 518
Size: 2042
Fragments arranged by size:
2042 1020 882 648 359 231 174 159
132
MspA1I CmG'CkG
Cuts at: 1606 2080 2163 2248 2308 2699 3063 3740 4888
Size: 474 83 85 60 391 364 677 1148
Cuts at: 4888 5133 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 474 391 364 245 85
83 60
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5095
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5647
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 2506 3005 4550 1132
Size: 97 1277 499 1545 2229
Fragments arranged by size:
2229 1545 1277 499 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5647
PciI A'CATG_T
Cuts at: 4546 4546
Size: 5647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5647
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 5229 5261 3716
Size: 32 1481 32 4102
Fragments arranged by size:
4102 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5647
PsiI TTA'TAA
Cuts at: 3217 3217
Size: 5647
PstI C_TGCA'G
Cuts at: 830 2999 830
Size: 2169 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
PvuII CAG'CTG
Cuts at: 2080 2163 2248 2308 2699 2080
Size: 83 85 60 391 5028
Fragments arranged by size:
5028 391 85 83 60
SacI G_AGCT'C
Cuts at: 2076 2076
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SbfI CC_TGCA'GG
Cuts at: 2999 2999
Size: 5647
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SfcI C'TryA_G
Cuts at: 826 1045 2872 2995 4130 4811 5002 826
Size: 219 1827 123 1135 681 191 1471
Fragments arranged by size:
1827 1471 1135 681 219 191 123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2974 1872
Size: 1102 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3751 4652 4914 820
Size: 197 926 1808 901 262 1553
Fragments arranged by size:
1808 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 3005 1132
Size: 1873 3774
SspI AAT'ATT
Cuts at: 5 52 3571 5
Size: 47 3519 2081
Fragments arranged by size:
3519 2081 47
StuI AGG'CCT
Cuts at: 1517 2714 1517
Size: 1197 4450
StyI C'CwwG_G
Cuts at: 513 1065 2320 3086 513
Size: 552 1255 766 3074
Fragments arranged by size:
3074 1255 766 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2318 2597 3674 3691 3844 4029 4474
Size: 1255 738 279 1077 17 153 185 445
Cuts at: 4474 325
Size: 1498
Fragments arranged by size:
1498 1255 1077 738 445 279 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3893 5605 96
Size: 274 80 33 51 1443 1916 1712 138
Fragments arranged by size:
1916 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2028 2060 2913 2945 5607 5639 2028
Size: 32 853 32 2662 32 2036
Fragments arranged by size:
2662 2036 853 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5647
XbaI T'CTAG_A
Cuts at: 2983 2983
Size: 5647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2287 2287
Size: 5647
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5647
XmaI C'CCGG_G
Cuts at: 1870 2972 1870
Size: 1102 4545
XmnI GAAnn'nnTTC
Cuts at: 3776 3776
Size: 5647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI