Restriction map for
pFN21AE0293
(Circular) (Six-base) MAPSORT of: pFN21AE0293.seq Check: 5129 from: 1 to: 5692
pFN21AE0293 5692 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 278
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2574 3035 1937
Size: 637 461 4594
Fragments arranged by size:
4594 637 461
Acc65I G'GTAC_C
Cuts at: 3013 3013
Size: 5692
AclI AA'CG_TT
Cuts at: 1563 3819 4192 1563
Size: 2256 373 3063
Fragments arranged by size:
3063 2256 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2366 3755 5139 5407 1632
Size: 734 1389 1384 268 1917
Fragments arranged by size:
1917 1389 1384 734 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5495
AflIII A'CryG_T
Cuts at: 1203 4591 5568 1203
Size: 3388 977 1327
Fragments arranged by size:
3388 1327 977
AgeI A'CCGG_T
Cuts at: 4523 4523
Size: 5692
AhdI GACnn_n'nnGTC
Cuts at: 4421 4421
Size: 5692
AleI CACnn'nnGTG
Cuts at: 1166 2141 1166
Size: 975 4717
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2525 1184
Size: 1341 4351
AlwNI CAG_nnn'CTG
Cuts at: 2586 3435 5007 2586
Size: 849 1572 3271
Fragments arranged by size:
3271 1572 849
ApaI G_GGCC'C
Cuts at: 2559 2559
Size: 5692
ApaLI G'TGCA_C
Cuts at: 3749 4905 3749
Size: 1156 4536
ApoI r'AATT_y
Cuts at: 1493 3001 3227 3379 5488 1493
Size: 1508 226 152 2109 1697
Fragments arranged by size:
2109 1697 1508 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 2071 3017 1870
Size: 73 128 946 4545
Fragments arranged by size:
4545 946 128 73
BamHI G'GATC_C
Cuts at: 3022 3389 3022
Size: 367 5325
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2300 2379 3013 4468 618
Size: 325 217 627 513 79 634 1455 1842
Fragments arranged by size:
1842 1455 634 627 513 325 217 79
BanII G_rGCy'C
Cuts at: 1391 2078 2559 2683 3011 1391
Size: 687 481 124 328 4072
Fragments arranged by size:
4072 687 481 328 124
BbeI G_GCGC'C
Cuts at: 2304 2304
Size: 5692
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4721
BbvCI CC'TCA_GC
Cuts at: 2830 2830
Size: 5692
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2710 2744 3883 3917 1903
Size: 34 773 34 1139 34 3678
Fragments arranged by size:
3678 1139 773 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3584 4794 3584
Size: 1210 4482
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4303 136
Size: 107 122 71 631 605 2631 1525
Fragments arranged by size:
2631 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5687 5687
Size: 5692
BlpI GC'TnA_GC
Cuts at: 2387 2454 3109 2387
Size: 67 655 4970
Fragments arranged by size:
4970 655 67
Bme1580I G_kGCm'C
Cuts at: 1790 2559 3753 4909 1790
Size: 769 1194 1156 2573
Fragments arranged by size:
2573 1194 1156 769
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2474 3423 4381
Size: 416 53 83 399 24 1057 949 958
Cuts at: 4381 442
Size: 1753
Fragments arranged by size:
1753 1057 958 949 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2480 2780 4352 1239
Size: 1241 300 1572 2579
Fragments arranged by size:
2579 1572 1241 300
Bpu10I CC'TnA_GC
Cuts at: 2830 2830
Size: 5692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3817 4682 4980 3817
Size: 865 298 4529
Fragments arranged by size:
4529 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4355 882
Size: 617 2856 2219
Fragments arranged by size:
2856 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3483 493
Size: 711 2279 2702
Fragments arranged by size:
2702 2279 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2301 3499 3881 4516
Size: 53 83 186 936 768 1198 382 635
Cuts at: 4516 275
Size: 1451
Fragments arranged by size:
1451 1198 936 768 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4125 4523 4797 4944 5355 4125
Size: 398 274 147 411 4462
Fragments arranged by size:
4462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5467
Fragments arranged by size:
5467 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2764 1186
Size: 1578 4114
BseYI C'CCAG_C
Cuts at: 2596 4895 5391 2596
Size: 2299 496 2897
Fragments arranged by size:
2897 2299 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2415 1163
Size: 573 679 4440
Fragments arranged by size:
4440 679 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2052 2904 3142 3903 4931 1540
Size: 275 186 51 852 238 761 1028 2301
Fragments arranged by size:
2301 1028 852 761 275 238 186 51
BsiHKAI G_wGCw'C
Cuts at: 2715 3753 3838 4909 2715
Size: 1038 85 1071 3498
Fragments arranged by size:
3498 1071 1038 85
BsmI GAATG_Cn'
Cuts at: 3203 3296 3203
Size: 93 5599
BsmBI CGTCTCn'nnnn_
Cuts at: 2911 2911
Size: 5692
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2559 2683 2715 3011 3753 3838
Size: 399 288 481 124 32 296 742 85
Cuts at: 3838 4909 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 481 399 296 288 124
85 32
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 3581 5311 3581
Size: 1730 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3047 844
Size: 2203 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3579 1394
Size: 429 1756 3507
Fragments arranged by size:
3507 1756 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2235 4187 4361 65
Size: 1183 987 1952 174 1396
Fragments arranged by size:
1952 1396 1183 987 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2545 4336 4523 1700
Size: 110 735 1791 187 2869
Fragments arranged by size:
2869 1791 735 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5692
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 3752 4764 3752
Size: 1012 4680
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5692
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 2287 1184
Size: 1103 4589
BstXI CCAn_nnnn'nTGG
Cuts at: 2284 2569 2284
Size: 285 5407
BstYI r'GATC_y
Cuts at: 1972 3022 3389 3774 3791 5243 5329 5341 5687
Size: 1050 367 385 17 1452 86 12 346
Cuts at: 5687 1972
Size: 1977
Fragments arranged by size:
1977 1452 1050 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2277 2551 2910 513
Size: 552 939 273 274 359 3295
Fragments arranged by size:
3295 939 552 359 274 273
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4953
Fragments arranged by size:
4953 683 56
BtsI GCAGTG_nn'
Cuts at: 3208 4002 4022 3208
Size: 794 20 4878
Fragments arranged by size:
4878 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2996 2996
Size: 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2106 2239
Size: 54 1425 50 275 18 219 57 133
Cuts at: 2239 2706 3139 4028 5657 8
Size: 467 433 889 1629 43
Fragments arranged by size:
1629 1425 889 467 433 275 219 133
57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2049 3139 1537
Size: 275 237 1090 4090
Fragments arranged by size:
4090 1090 275 237
EarI CTCTTCn'nnn_
Cuts at: 1353 2846 3622 1353
Size: 1493 776 3423
Fragments arranged by size:
3423 1493 776
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4279 4653 4799 387
Size: 1064 138 2690 374 146 1280
Fragments arranged by size:
2690 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2366 2480 2780 3755 4352 5139 5407
Size: 393 734 114 300 975 597 787 268
Cuts at: 5407 1239
Size: 1524
Fragments arranged by size:
1524 975 787 734 597 393 300 268
114
EcoNI CCTnn'n_nnAGG
Cuts at: 2927 2927
Size: 5692
EcoRI G'AATT_C
Cuts at: 3001 3379 3001
Size: 378 5314
FspI TGC'GCA
Cuts at: 4198 4198
Size: 5692
HaeII r_GCGC'y
Cuts at: 1678 2304 2759 4839 1678
Size: 626 455 2080 2531
Fragments arranged by size:
2531 2080 626 455
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4333 4365 4407 4439 1373
Size: 32 135 32 2761 32 42 32 2626
Fragments arranged by size:
2761 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3036 3282 669
Size: 988 281 1098 246 3079
Fragments arranged by size:
3079 1098 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KasI G'GCGC_C
Cuts at: 2300 2300
Size: 5692
KpnI G_GTAC'C
Cuts at: 3017 3017
Size: 5692
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4806 4990 1305
Size: 3501 184 2007
Fragments arranged by size:
3501 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2241 10
Size: 54 1425 343 409 3461
Fragments arranged by size:
3461 1425 409 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 3650 4009 4168 518
Size: 648 975 1509 359 159 2042
Fragments arranged by size:
2042 1509 975 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2027 3108 3785 4933 5178 1606
Size: 412 9 1081 677 1148 245 2120
Fragments arranged by size:
2120 1148 1081 677 412 245 9
NaeI GCC'GGC
Cuts at: 2547 2547
Size: 5692
NarI GG'CG_CC
Cuts at: 2301 2301
Size: 5692
NcoI C'CATG_G
Cuts at: 513 1065 2004 2551 513
Size: 552 939 547 3654
Fragments arranged by size:
3654 939 552 547
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5692
NgoMIV G'CCGG_C
Cuts at: 2545 2545
Size: 5692
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 3139 3139
Size: 5692
NspI r_CATG'y
Cuts at: 1132 1229 3050 4595 1132
Size: 97 1821 1545 2229
Fragments arranged by size:
2229 1821 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2296 1348
Size: 948 4744
PciI A'CATG_T
Cuts at: 4591 4591
Size: 5692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5692
PfoI T'CCnGG_A
Cuts at: 2844 2844
Size: 5692
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5692
PsiI TTA'TAA
Cuts at: 3262 3262
Size: 5692
PspOMI G'GGCC_C
Cuts at: 2555 2555
Size: 5692
PstI C_TGCA'G
Cuts at: 830 2017 2723 3044 830
Size: 1187 706 321 3478
Fragments arranged by size:
3478 1187 706 321
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
PvuII CAG'CTG
Cuts at: 2018 2018
Size: 5692
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SbfI CC_TGCA'GG
Cuts at: 3044 3044
Size: 5692
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SfcI C'TryA_G
Cuts at: 826 1045 2013 2147 2719 3040 4175 4856 5047
Size: 219 968 134 572 321 1135 681 191
Cuts at: 5047 826
Size: 1471
Fragments arranged by size:
1471 1135 968 681 572 321 219 191
134
SfoI GGC'GCC
Cuts at: 2302 2302
Size: 5692
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 3019 1872
Size: 1147 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3796 4697 4959 820
Size: 197 926 1853 901 262 1553
Fragments arranged by size:
1853 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 3050 1132
Size: 1918 3774
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5692
StyI C'CwwG_G
Cuts at: 513 1065 2004 2305 2551 2622 2956 3131 513
Size: 552 939 301 246 71 334 175 3074
Fragments arranged by size:
3074 939 552 334 301 246 175 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3719 3736 3889 4074 4519 325
Size: 1255 2139 17 153 185 445 1498
Fragments arranged by size:
2139 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3938 5650 96
Size: 274 80 33 51 1443 1961 1712 138
Fragments arranged by size:
1961 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2875 2907 5652 5684 2875
Size: 32 2745 32 2883
Fragments arranged by size:
2883 2745 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5692
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2566 2566
Size: 5692
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 3017 1870
Size: 1147 4545
XmnI GAAnn'nnTTC
Cuts at: 3821 3821
Size: 5692
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 276
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI