Restriction map for
pFN21AE0292
(Circular) (Six-base) MAPSORT of: pFN21AE0292.seq Check: 1422 from: 1 to: 5461
pFN21AE0292 5461 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2305 2305
Size: 5461
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3271 278
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2493 2804 1937
Size: 556 311 4594
Fragments arranged by size:
4594 556 311
Acc65I G'GTAC_C
Cuts at: 2782 2782
Size: 5461
AclI AA'CG_TT
Cuts at: 1563 3588 3961 1563
Size: 2025 373 3063
Fragments arranged by size:
3063 2025 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2710 3524 4908 5176 1632
Size: 1078 814 1384 268 1917
Fragments arranged by size:
1917 1384 1078 814 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5264
AflIII A'CryG_T
Cuts at: 1203 4360 5337 1203
Size: 3157 977 1327
Fragments arranged by size:
3157 1327 977
AgeI A'CCGG_T
Cuts at: 4292 4292
Size: 5461
AhdI GACnn_n'nnGTC
Cuts at: 4190 4190
Size: 5461
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5461
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2624 1184
Size: 1440 4021
AlwNI CAG_nnn'CTG
Cuts at: 2623 3204 4776 2623
Size: 581 1572 3308
Fragments arranged by size:
3308 1572 581
ApaLI G'TGCA_C
Cuts at: 2084 2513 3518 4674 2084
Size: 429 1005 1156 2871
Fragments arranged by size:
2871 1156 1005 429
ApoI r'AATT_y
Cuts at: 1493 2770 2996 3148 5257 1493
Size: 1277 226 152 2109 1697
Fragments arranged by size:
2109 1697 1277 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4015 160
Size: 872 2983 1606
Fragments arranged by size:
2983 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
AvaI C'yCGr_G
Cuts at: 1870 1943 2060 2124 2175 2280 2786 1870
Size: 73 117 64 51 105 506 4545
Fragments arranged by size:
4545 506 117 105 73 64 51
BamHI G'GATC_C
Cuts at: 2791 3158 2791
Size: 367 5094
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2276 2330 2410 2444 2482
Size: 325 217 627 489 54 80 34 38
Cuts at: 2482 2782 4237 618
Size: 300 1455 1842
Fragments arranged by size:
1842 1455 627 489 325 300 217 80
54 38 34
BanII G_rGCy'C
Cuts at: 1391 2780 1391
Size: 1389 4072
BbeI G_GCGC'C
Cuts at: 2280 2334 2448 2280
Size: 54 114 5293
Fragments arranged by size:
5293 114 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4490
BbvCI CC'TCA_GC
Cuts at: 2501 2501
Size: 5461
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3652 3686 1903
Size: 34 1715 34 3678
Fragments arranged by size:
3678 1715 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3353 4563 3353
Size: 1210 4251
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5461
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2074 2292 2301
Size: 107 122 71 631 605 402 218 9
Cuts at: 2301 4072 136
Size: 1771 1525
Fragments arranged by size:
1771 1525 631 605 402 218 122 107
71 9
BglII A'GATC_T
Cuts at: 5456 5456
Size: 5461
BlpI GC'TnA_GC
Cuts at: 2878 2878
Size: 5461
Bme1580I G_kGCm'C
Cuts at: 1790 2088 2415 2487 2517 3522 4678 1790
Size: 298 327 72 30 1005 1156 2573
Fragments arranged by size:
2573 1156 1005 327 298 72 30
BmgBI CAC'GTC
Cuts at: 1318 2345 1318
Size: 1027 4434
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3192 4150 442
Size: 416 53 83 399 24 1775 958 1753
Fragments arranged by size:
1775 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5461
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4121 1239
Size: 2882 2579
Bpu10I CC'TnA_GC
Cuts at: 2501 2501
Size: 5461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3586 4451 4749 3586
Size: 865 298 4298
Fragments arranged by size:
4298 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2528 2703 4124 882
Size: 617 1029 175 1421 2219
Fragments arranged by size:
2219 1421 1029 617 175
BsaAI yAC'GTr
Cuts at: 493 1204 3252 493
Size: 711 2048 2702
Fragments arranged by size:
2702 2048 711
BsaBI GATnn'nnATC
Cuts at: 5455 5455
Size: 5461
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2232 2277 2331 2445
Size: 53 83 186 936 699 45 54 114
Cuts at: 2445 2490 2649 3268 3650 4285 275
Size: 45 159 619 382 635 1451
Fragments arranged by size:
1451 936 699 635 619 382 186 159
114 83 54 53 45 45
BsaWI w'CCGG_w
Cuts at: 3894 4292 4566 4713 5124 3894
Size: 398 274 147 411 4231
Fragments arranged by size:
4231 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2065 2095 1178
Size: 30 165 30 662 30 4544
Fragments arranged by size:
4544 662 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5461
BseYI C'CCAG_C
Cuts at: 2591 4664 5160 2591
Size: 2073 496 2892
Fragments arranged by size:
2892 2073 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2369 2461 2528 1163
Size: 573 633 92 67 4096
Fragments arranged by size:
4096 633 573 92 67
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2574 2680 2751 2911 3672 4700
Size: 275 186 573 106 71 160 761 1028
Cuts at: 4700 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 573 275 186 160 106
71
BsiHKAI G_wGCw'C
Cuts at: 2088 2517 2580 2706 3522 3607 4678 2088
Size: 429 63 126 816 85 1071 2871
Fragments arranged by size:
2871 1071 816 429 126 85 63
BsmI GAATG_Cn'
Cuts at: 2972 3065 2972
Size: 93 5368
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2415 2487 2517 2580 2706 2780
Size: 399 298 327 72 30 63 126 74
Cuts at: 2780 3522 3607 4678 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 327 298 126 85
74 72 63 30
BspEI T'CCGG_A
Cuts at: 5124 5124
Size: 5461
BspHI T'CATG_A
Cuts at: 3350 5080 3350
Size: 1730 3731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2305 2550 2816 844
Size: 1461 245 266 3489
Fragments arranged by size:
3489 1461 266 245
BsrBI CCG'CTC
Cuts at: 1394 1823 2256 3348 1394
Size: 429 433 1092 3507
Fragments arranged by size:
3507 1092 433 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3956 4130 65
Size: 1183 2708 174 1396
Fragments arranged by size:
2708 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2228 2246 2370 2559 2746 4105 4292
Size: 110 418 18 124 189 187 1359 187
Cuts at: 4292 1700
Size: 2869
Fragments arranged by size:
2869 1359 418 189 187 187 124 110
18
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5461
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5461
BssSI C'ACGA_G
Cuts at: 3521 4533 3521
Size: 1012 4449
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2551 794
Size: 1757 3704
BstBI TT'CG_AA
Cuts at: 4265 4265
Size: 5461
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5461
BstYI r'GATC_y
Cuts at: 1972 2791 3158 3543 3560 5012 5098 5110 5456
Size: 819 367 385 17 1452 86 12 346
Cuts at: 5456 1972
Size: 1977
Fragments arranged by size:
1977 1452 819 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2046 2364 2460 2568 513
Size: 552 939 42 318 96 108 3406
Fragments arranged by size:
3406 939 552 318 108 96 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4722
Fragments arranged by size:
4722 683 56
BtsI GCAGTG_nn'
Cuts at: 2033 2086 2977 3771 3791 2033
Size: 53 891 794 20 3703
Fragments arranged by size:
3703 891 794 53 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5461
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5426
DraI TTT'AAA
Cuts at: 2765 2765
Size: 5461
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4468 809
Size: 507 3152 1802
Fragments arranged by size:
3152 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2457 2571 2731
Size: 54 1425 50 275 18 627 114 160
Cuts at: 2731 2748 2908 3797 5426 8
Size: 17 160 889 1629 43
Fragments arranged by size:
1629 1425 889 627 275 160 160 114
54 50 43 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 2571 2748 2908 1537
Size: 275 759 177 160 4090
Fragments arranged by size:
4090 759 275 177 160
EarI CTCTTCn'nnn_
Cuts at: 1353 3391 1353
Size: 2038 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4048 4422 4568 387
Size: 1064 138 2459 374 146 1280
Fragments arranged by size:
2459 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2710 3524 4121 4908 5176 1239
Size: 393 1078 814 597 787 268 1524
Fragments arranged by size:
1524 1078 814 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2052 2076 2140 2155 2284 2380 2052
Size: 24 64 15 129 96 5133
Fragments arranged by size:
5133 129 96 64 24 15
EcoRI G'AATT_C
Cuts at: 2770 3148 2770
Size: 378 5083
FspI TGC'GCA
Cuts at: 2663 3967 2663
Size: 1304 4157
FspAI rTGC'GCAy
Cuts at: 2663 2663
Size: 5461
HaeII r_GCGC'y
Cuts at: 1678 2280 2334 2448 4608 1678
Size: 602 54 114 2160 2531
Fragments arranged by size:
2531 2160 602 114 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4102 4134 4176 4208 1373
Size: 32 135 32 2530 32 42 32 2626
Fragments arranged by size:
2626 2530 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2805 3051 669
Size: 988 281 867 246 3079
Fragments arranged by size:
3079 988 867 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5461
HpaI GTT'AAC
Cuts at: 3051 3051
Size: 5461
KasI G'GCGC_C
Cuts at: 2276 2330 2444 2276
Size: 54 114 5293
Fragments arranged by size:
5293 114 54
KpnI G_GTAC'C
Cuts at: 2786 2786
Size: 5461
MfeI C'AATT_G
Cuts at: 3060 3060
Size: 5461
MluI A'CGCG_T
Cuts at: 5337 5337
Size: 5461
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2418 4575 4759 1305
Size: 1113 2157 184 2007
Fragments arranged by size:
2157 2007 1113 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3639
Fragments arranged by size:
3639 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3419 3778 3937 518
Size: 648 2253 359 159 2042
Fragments arranged by size:
2253 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2462 2570 2730 2877 3554 4702 4947 1606
Size: 856 108 160 147 677 1148 245 2120
Fragments arranged by size:
2120 1148 856 677 245 160 147 108
NaeI GCC'GGC
Cuts at: 2230 2248 2372 2748 2230
Size: 18 124 376 4943
Fragments arranged by size:
4943 376 124 18
NarI GG'CG_CC
Cuts at: 2277 2331 2445 2277
Size: 54 114 5293
Fragments arranged by size:
5293 114 54
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3970
Fragments arranged by size:
3970 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5461
NgoMIV G'CCGG_C
Cuts at: 2228 2246 2370 2746 2228
Size: 18 124 376 4943
Fragments arranged by size:
4943 376 124 18
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5461
NotI GC'GGCC_GC
Cuts at: 2908 2908
Size: 5461
NspI r_CATG'y
Cuts at: 1132 1229 2668 2819 4364 1132
Size: 97 1439 151 1545 2229
Fragments arranged by size:
2229 1545 1439 151 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5461
PciI A'CATG_T
Cuts at: 4360 4360
Size: 5461
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5461
PmeI GTTT'AAAC
Cuts at: 2765 2765
Size: 5461
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3530 3562 5043 5075 3530
Size: 32 1481 32 3916
Fragments arranged by size:
3916 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2140 2155 2140
Size: 15 5446
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5461
PsiI TTA'TAA
Cuts at: 3031 3031
Size: 5461
PspXI vC'TCGA_Gb
Cuts at: 2175 2175
Size: 5461
PstI C_TGCA'G
Cuts at: 830 2292 2813 830
Size: 1462 521 3478
Fragments arranged by size:
3478 1462 521
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5461
SacII CC_GC'GG
Cuts at: 2463 2571 2463
Size: 108 5353
SalI G'TCGA_C
Cuts at: 1936 2803 1936
Size: 867 4594
SbfI CC_TGCA'GG
Cuts at: 2292 2813 2292
Size: 521 4940
ScaI AGT'ACT
Cuts at: 3709 5421 3709
Size: 1712 3749
SexAI A'CCwGG_T
Cuts at: 2736 2736
Size: 5461
SfcI C'TryA_G
Cuts at: 826 1045 2190 2288 2809 3944 4625 4816 826
Size: 219 1145 98 521 1135 681 191 1471
Fragments arranged by size:
1471 1145 1135 681 521 219 191 98
SfiI GGCCn_nnn'nGGCC
Cuts at: 2292 2301 2292
Size: 9 5452
SfoI GGC'GCC
Cuts at: 2278 2332 2446 2278
Size: 54 114 5293
Fragments arranged by size:
5293 114 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5461
SmaI CCC'GGG
Cuts at: 1872 2062 2126 2282 2788 1872
Size: 190 64 156 506 4545
Fragments arranged by size:
4545 506 190 156 64
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2175 3565 4466 4728 820
Size: 197 926 232 1390 901 262 1553
Fragments arranged by size:
1553 1390 926 901 262 232 197
SnaBI TAC'GTA
Cuts at: 493 3252 493
Size: 2759 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5461
SphI G_CATG'C
Cuts at: 1132 2819 1132
Size: 1687 3774
SrfI GCCC'GGGC
Cuts at: 2062 2062
Size: 5461
SspI AAT'ATT
Cuts at: 5 52 3385 5
Size: 47 3333 2081
Fragments arranged by size:
3333 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5461
StyI C'CwwG_G
Cuts at: 513 1065 2004 2900 513
Size: 552 939 896 3074
Fragments arranged by size:
3074 939 896 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2647 3488 3505 3658 3843 4288 325
Size: 1255 1067 841 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1067 841 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3707 5419 96
Size: 274 80 33 51 1443 1730 1712 138
Fragments arranged by size:
1730 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2065 2097 5421 5453 2065
Size: 32 3324 32 2073
Fragments arranged by size:
3324 2073 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2651 1316
Size: 1335 4126
XbaI T'CTAG_A
Cuts at: 2797 2797
Size: 5461
XhoI C'TCGA_G
Cuts at: 1943 2175 1943
Size: 232 5229
XmaI C'CCGG_G
Cuts at: 1870 2060 2124 2280 2786 1870
Size: 190 64 156 506 4545
Fragments arranged by size:
4545 506 190 156 64
XmnI GAAnn'nnTTC
Cuts at: 3590 3590
Size: 5461
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3269 276
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacII
SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI NruI NsiI PacI PfoI PmlI
PspOMI PsrI PvuII RsrII SacI SanDI SapI SwaI
XcmI