Restriction map for
pFN21AE0290
(Circular) (Six-base) MAPSORT of: pFN21AE0290.seq Check: 5309 from: 1 to: 5581
pFN21AE0290 5581 bp
With 182 enzymes: SgfI *
December 8, 2014 13:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2629 2629
Size: 5581
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3391 278
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2924 1937
Size: 987 4594
Acc65I G'GTAC_C
Cuts at: 2902 2902
Size: 5581
AclI AA'CG_TT
Cuts at: 1563 3708 4081 1563
Size: 2145 373 3063
Fragments arranged by size:
3063 2145 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2107 2645 3644 5028 5296 1632
Size: 475 538 999 1384 268 1917
Fragments arranged by size:
1917 1384 999 538 475 268
AfeI AGC'GCT
Cuts at: 2444 2444
Size: 5581
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5384
AflIII A'CryG_T
Cuts at: 1203 2745 2747 4480 5457 1203
Size: 1542 2 1733 977 1327
Fragments arranged by size:
1733 1542 1327 977 2
AgeI A'CCGG_T
Cuts at: 4412 4412
Size: 5581
AhdI GACnn_n'nnGTC
Cuts at: 4310 4310
Size: 5581
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5581
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2891 1184
Size: 1707 3874
AlwNI CAG_nnn'CTG
Cuts at: 2130 3324 4896 2130
Size: 1194 1572 2815
Fragments arranged by size:
2815 1572 1194
ApaI G_GGCC'C
Cuts at: 2105 2417 2563 2708 2105
Size: 312 146 145 4978
Fragments arranged by size:
4978 312 146 145
ApaLI G'TGCA_C
Cuts at: 3638 4794 3638
Size: 1156 4425
ApoI r'AATT_y
Cuts at: 1493 2890 3116 3268 5377 1493
Size: 1397 226 152 2109 1697
Fragments arranged by size:
2109 1697 1397 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4135 160
Size: 872 3103 1606
Fragments arranged by size:
3103 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
AvaI C'yCGr_G
Cuts at: 1870 1943 2098 2358 2630 2906 1870
Size: 73 155 260 272 276 4545
Fragments arranged by size:
4545 276 272 260 155 73
BamHI G'GATC_C
Cuts at: 2911 3278 2911
Size: 367 5214
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2023 2762 2902 4357 618
Size: 325 217 627 236 739 140 1455 1842
Fragments arranged by size:
1842 1455 739 627 325 236 217 140
BanII G_rGCy'C
Cuts at: 1391 2105 2151 2417 2462 2563 2583 2708 2900
Size: 714 46 266 45 101 20 125 192
Cuts at: 2900 1391
Size: 4072
Fragments arranged by size:
4072 714 266 192 125 101 46 45
20
BbeI G_GCGC'C
Cuts at: 2027 2766 2027
Size: 739 4842
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3772 3806 1903
Size: 34 1835 34 3678
Fragments arranged by size:
3678 1835 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3473 4683 3473
Size: 1210 4371
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5581
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2603 2865
Size: 107 122 71 631 605 337 594 262
Cuts at: 2865 4192 136
Size: 1327 1525
Fragments arranged by size:
1525 1327 631 605 594 337 262 122
107 71
BglII A'GATC_T
Cuts at: 5576 5576
Size: 5581
BlpI GC'TnA_GC
Cuts at: 2456 2998 2456
Size: 542 5039
Bme1580I G_kGCm'C
Cuts at: 1790 2076 2105 2417 2563 2708 3642 4798 1790
Size: 286 29 312 146 145 934 1156 2573
Fragments arranged by size:
2573 1156 934 312 286 146 145 29
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5581
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3312 4270 442
Size: 416 53 83 399 24 1895 958 1753
Fragments arranged by size:
1895 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2420 2456 4241 1239
Size: 1181 36 1785 2579
Fragments arranged by size:
2579 1785 1181 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2684 3706 4571 4869 2684
Size: 1022 865 298 3396
Fragments arranged by size:
3396 1022 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4244 882
Size: 617 2745 2219
Fragments arranged by size:
2745 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2089 2748 3372 493
Size: 711 885 659 624 2702
Fragments arranged by size:
2702 885 711 659 624
BsaBI GATnn'nnATC
Cuts at: 5575 5575
Size: 5581
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2024 2543 2763 3388
Size: 53 83 186 936 491 519 220 625
Cuts at: 3388 3770 4405 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 635 625 519 491 382 220
186 83 53
BsaWI w'CCGG_w
Cuts at: 4014 4412 4686 4833 5244 4014
Size: 398 274 147 411 4351
Fragments arranged by size:
4351 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2582 2612 1178
Size: 30 165 30 1179 30 4147
Fragments arranged by size:
4147 1179 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2227 2230 2233 2453 2567 1186
Size: 1041 3 3 220 114 4200
Fragments arranged by size:
4200 1041 220 114 3 3
BseYI C'CCAG_C
Cuts at: 2075 2290 2302 2491 4784 5280 2075
Size: 215 12 189 2293 496 2376
Fragments arranged by size:
2376 2293 496 215 189 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2573 1163
Size: 573 837 4171
Fragments arranged by size:
4171 837 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2047 2577 2613 3031 3792 4820
Size: 275 186 46 530 36 418 761 1028
Cuts at: 4820 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 530 418 275 186 46
36
BsiHKAI G_wGCw'C
Cuts at: 3642 3727 4798 3642
Size: 85 1071 4425
Fragments arranged by size:
4425 1071 85
BsmI GAATG_Cn'
Cuts at: 3092 3185 3092
Size: 93 5488
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2105 2151 2417 2462 2563 2583
Size: 399 286 29 46 266 45 101 20
Cuts at: 2583 2708 2900 3642 3727 4798 1391
Size: 125 192 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 286 266 192 125
101 85 46 45 29 20
BspEI T'CCGG_A
Cuts at: 5244 5244
Size: 5581
BspHI T'CATG_A
Cuts at: 3470 5200 3470
Size: 1730 3851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2629 2936 844
Size: 1785 307 3489
Fragments arranged by size:
3489 1785 307
BsrBI CCG'CTC
Cuts at: 1394 1823 2031 2557 2803 3468 1394
Size: 429 208 526 246 665 3507
Fragments arranged by size:
3507 665 526 429 246 208
BsrDI GCAATG_nn'
Cuts at: 65 1248 4076 4250 65
Size: 1183 2828 174 1396
Fragments arranged by size:
2828 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2296 4225 4412 1700
Size: 110 486 1929 187 2869
Fragments arranged by size:
2869 1929 486 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5581
BssHII G'CGCG_C
Cuts at: 1925 2379 2392 2394 2499 1925
Size: 454 13 2 105 5007
Fragments arranged by size:
5007 454 105 13 2
BssSI C'ACGA_G
Cuts at: 2069 3641 4653 2069
Size: 1572 1012 2997
Fragments arranged by size:
2997 1572 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5581
BstBI TT'CG_AA
Cuts at: 4385 4385
Size: 5581
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5581
BstXI CCAn_nnnn'nTGG
Cuts at: 2173 2173
Size: 5581
BstYI r'GATC_y
Cuts at: 1972 2911 3278 3663 3680 5132 5218 5230 5576
Size: 939 367 385 17 1452 86 12 346
Cuts at: 5576 1972
Size: 1977
Fragments arranged by size:
1977 1452 939 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2041 2061 2203 513
Size: 552 939 37 20 142 3891
Fragments arranged by size:
3891 939 552 142 37 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2687 487
Size: 683 56 1461 3381
Fragments arranged by size:
3381 1461 683 56
BtsI GCAGTG_nn'
Cuts at: 3097 3891 3911 3097
Size: 794 20 4767
Fragments arranged by size:
4767 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5581
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5546
DraI TTT'AAA
Cuts at: 2885 2885
Size: 5581
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4588 809
Size: 507 3272 1802
Fragments arranged by size:
3272 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2044 2574 2610
Size: 54 1425 50 275 18 214 530 36
Cuts at: 2610 3028 3917 5546 8
Size: 418 889 1629 43
Fragments arranged by size:
1629 1425 889 530 418 275 214 54
50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2044 2574 2610 3028 1537
Size: 275 232 530 36 418 4090
Fragments arranged by size:
4090 530 418 275 232 36
EarI CTCTTCn'nnn_
Cuts at: 1353 3511 1353
Size: 2158 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4168 4542 4688 387
Size: 1064 138 2579 374 146 1280
Fragments arranged by size:
2579 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2107 2420 2456 2645 3644 4241 5028
Size: 393 475 313 36 189 999 597 787
Cuts at: 5028 5296 1239
Size: 268 1524
Fragments arranged by size:
1524 999 787 597 475 393 313 268
189 36
EcoO109I rG'GnC_Cy
Cuts at: 2355 2414 2560 2704 2705 2355
Size: 59 146 144 1 5231
Fragments arranged by size:
5231 146 144 59 1
EcoRI G'AATT_C
Cuts at: 2890 3268 2890
Size: 378 5203
FspI TGC'GCA
Cuts at: 4087 4087
Size: 5581
HaeII r_GCGC'y
Cuts at: 1678 2027 2069 2082 2097 2291 2303 2446 2681
Size: 349 42 13 15 194 12 143 235
Cuts at: 2681 2766 2850 4728 1678
Size: 85 84 1878 2531
Fragments arranged by size:
2531 1878 349 235 194 143 85 84
42 15 13 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2339 2371 4222 4254 4296
Size: 32 135 32 767 32 1851 32 42
Cuts at: 4296 4328 1373
Size: 32 2626
Fragments arranged by size:
2626 1851 767 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2925 3171 669
Size: 988 281 987 246 3079
Fragments arranged by size:
3079 988 987 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5581
HpaI GTT'AAC
Cuts at: 3171 3171
Size: 5581
KasI G'GCGC_C
Cuts at: 2023 2762 2023
Size: 739 4842
KpnI G_GTAC'C
Cuts at: 2906 2906
Size: 5581
MfeI C'AATT_G
Cuts at: 3180 3180
Size: 5581
MluI A'CGCG_T
Cuts at: 5457 5457
Size: 5581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4695 4879 1305
Size: 3390 184 2007
Fragments arranged by size:
3390 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3759
Fragments arranged by size:
3759 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3539 3898 4057 518
Size: 648 2373 359 159 2042
Fragments arranged by size:
2373 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2043 2205 2769 2997 3674 4822 5067 1606
Size: 437 162 564 228 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 564 437 245 228 162
NaeI GCC'GGC
Cuts at: 2298 2298
Size: 5581
NarI GG'CG_CC
Cuts at: 2024 2763 2024
Size: 739 4842
NcoI C'CATG_G
Cuts at: 513 1065 2004 2061 513
Size: 552 939 57 4033
Fragments arranged by size:
4033 939 552 57
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5581
NgoMIV G'CCGG_C
Cuts at: 2296 2296
Size: 5581
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5581
NotI GC'GGCC_GC
Cuts at: 2044 2610 3028 2044
Size: 566 418 4597
Fragments arranged by size:
4597 566 418
NspI r_CATG'y
Cuts at: 1132 1229 2939 4484 1132
Size: 97 1710 1545 2229
Fragments arranged by size:
2229 1710 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2019 1348
Size: 671 4910
PciI A'CATG_T
Cuts at: 4480 4480
Size: 5581
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5581
PfoI T'CCnGG_A
Cuts at: 2129 2129
Size: 5581
PmeI GTTT'AAAC
Cuts at: 2885 2885
Size: 5581
PmlI CAC'GTG
Cuts at: 2748 2748
Size: 5581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3650 3682 5163 5195 3650
Size: 32 1481 32 4036
Fragments arranged by size:
4036 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2355 2355
Size: 5581
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5581
PsiI TTA'TAA
Cuts at: 3151 3151
Size: 5581
PspOMI G'GGCC_C
Cuts at: 2101 2413 2559 2704 2101
Size: 312 146 145 4978
Fragments arranged by size:
4978 312 146 145
PstI C_TGCA'G
Cuts at: 830 2640 2933 830
Size: 1810 293 3478
Fragments arranged by size:
3478 1810 293
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5581
SacII CC_GC'GG
Cuts at: 2044 2206 2044
Size: 162 5419
SalI G'TCGA_C
Cuts at: 1936 2923 1936
Size: 987 4594
SanDI GG'GwC_CC
Cuts at: 2355 2355
Size: 5581
SbfI CC_TGCA'GG
Cuts at: 2933 2933
Size: 5581
ScaI AGT'ACT
Cuts at: 2795 3829 5541 2795
Size: 1034 1712 2835
Fragments arranged by size:
2835 1712 1034
SfcI C'TryA_G
Cuts at: 826 1045 2636 2929 4064 4745 4936 826
Size: 219 1591 293 1135 681 191 1471
Fragments arranged by size:
1591 1471 1135 681 293 219 191
SfoI GGC'GCC
Cuts at: 2025 2764 2025
Size: 739 4842
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5581
SmaI CCC'GGG
Cuts at: 1872 2100 2360 2908 1872
Size: 228 260 548 4545
Fragments arranged by size:
4545 548 260 228
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2699 3685 4586 4848 820
Size: 197 926 756 986 901 262 1553
Fragments arranged by size:
1553 986 926 901 756 262 197
SnaBI TAC'GTA
Cuts at: 493 3372 493
Size: 2879 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5581
SphI G_CATG'C
Cuts at: 1132 2939 1132
Size: 1807 3774
SspI AAT'ATT
Cuts at: 5 52 3505 5
Size: 47 3453 2081
Fragments arranged by size:
3453 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5581
StyI C'CwwG_G
Cuts at: 513 1065 2004 2061 3020 513
Size: 552 939 57 959 3074
Fragments arranged by size:
3074 959 939 552 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2705 3608 3625 3778 3963 4408 325
Size: 1255 1125 903 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1125 903 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2793 3827 5539
Size: 274 80 33 51 1443 816 1034 1712
Cuts at: 5539 96
Size: 138
Fragments arranged by size:
1712 1443 1034 816 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2582 2614 5541 5573 2582
Size: 32 2927 32 2590
Fragments arranged by size:
2927 2590 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5581
XbaI T'CTAG_A
Cuts at: 2917 2917
Size: 5581
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5581
XmaI C'CCGG_G
Cuts at: 1870 2098 2358 2906 1870
Size: 228 260 548 4545
Fragments arranged by size:
4545 548 260 228
XmnI GAAnn'nnTTC
Cuts at: 3710 3710
Size: 5581
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3389 276
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PspXI PsrI
PvuII RsrII SacI SapI SexAI SfiI SrfI SwaI
XcmI