Restriction map for
pFN21AE0283
(Circular) (Six-base) MAPSORT of: pFN21AE0283.seq Check: 6170 from: 1 to: 5674
pFN21AE0283 5674 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3484 278
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3017 1937
Size: 1080 4594
Acc65I G'GTAC_C
Cuts at: 2397 2995 2397
Size: 598 5076
AclI AA'CG_TT
Cuts at: 1563 3801 4174 1563
Size: 2238 373 3063
Fragments arranged by size:
3063 2238 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2579 3737 5121 5389 1632
Size: 947 1158 1384 268 1917
Fragments arranged by size:
1917 1384 1158 947 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5477
AflIII A'CryG_T
Cuts at: 1203 2577 2723 4573 5550 1203
Size: 1374 146 1850 977 1327
Fragments arranged by size:
1850 1374 1327 977 146
AgeI A'CCGG_T
Cuts at: 4505 4505
Size: 5674
AhdI GACnn_n'nnGTC
Cuts at: 2263 4403 2263
Size: 2140 3534
AleI CACnn'nnGTG
Cuts at: 1166 2780 1166
Size: 1614 4060
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5674
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2914 2946 2914
Size: 32 5642
AlwNI CAG_nnn'CTG
Cuts at: 3417 4989 3417
Size: 1572 4102
ApaLI G'TGCA_C
Cuts at: 3731 4887 3731
Size: 1156 4518
ApoI r'AATT_y
Cuts at: 1493 2336 2983 3209 3361 5470 1493
Size: 843 647 226 152 2109 1697
Fragments arranged by size:
2109 1697 843 647 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4228 160
Size: 872 3196 1606
Fragments arranged by size:
3196 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
AvaI C'yCGr_G
Cuts at: 1870 1943 2078 2517 2999 1870
Size: 73 135 439 482 4545
Fragments arranged by size:
4545 482 439 135 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2215 2248 2215
Size: 33 5641
BamHI G'GATC_C
Cuts at: 3004 3371 3004
Size: 367 5307
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2397 2995 4450 618
Size: 325 217 627 610 598 1455 1842
Fragments arranged by size:
1842 1455 627 610 598 325 217
BanII G_rGCy'C
Cuts at: 1391 2993 1391
Size: 1602 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2012 2092 928
Size: 971 113 80 4510
Fragments arranged by size:
4510 971 113 80
BbvCI CC'TCA_GC
Cuts at: 2697 2697
Size: 5674
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3865 3899 1903
Size: 34 1928 34 3678
Fragments arranged by size:
3678 1928 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2209 3566 4776 2209
Size: 1357 1210 3107
Fragments arranged by size:
3107 1357 1210
BclI T'GATC_A
Cuts at: 1548 2961 1548
Size: 1413 4261
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4285 136
Size: 107 122 71 631 605 2613 1525
Fragments arranged by size:
2613 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5669 5669
Size: 5674
BlpI GC'TnA_GC
Cuts at: 2195 3091 2195
Size: 896 4778
Bme1580I G_kGCm'C
Cuts at: 1790 3735 4891 1790
Size: 1945 1156 2573
Fragments arranged by size:
2573 1945 1156
BmgBI CAC'GTC
Cuts at: 1318 2580 1318
Size: 1262 4412
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3405 4363 442
Size: 416 53 83 399 24 1988 958 1753
Fragments arranged by size:
1988 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2009 2237 2790 4334 1239
Size: 770 228 553 1544 2579
Fragments arranged by size:
2579 1544 770 553 228
Bpu10I CC'TnA_GC
Cuts at: 2697 2697
Size: 5674
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2899 3799 4664 4962 2899
Size: 900 865 298 3611
Fragments arranged by size:
3611 900 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2357 2585 4337 882
Size: 617 858 228 1752 2219
Fragments arranged by size:
2219 1752 858 617 228
BsaAI yAC'GTr
Cuts at: 493 1204 2724 3465 493
Size: 711 1520 741 2702
Fragments arranged by size:
2702 1520 741 711
BsaBI GATnn'nnATC
Cuts at: 5668 5668
Size: 5674
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3481 3863 4498 275
Size: 53 83 186 936 1948 382 635 1451
Fragments arranged by size:
1948 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4107 4505 4779 4926 5337 4107
Size: 398 274 147 411 4444
Fragments arranged by size:
4444 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2147 2177 2914 2944 1178
Size: 30 165 30 744 30 737 30 3908
Fragments arranged by size:
3908 744 737 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2396 1186
Size: 1210 4464
BseYI C'CCAG_C
Cuts at: 2347 2548 4877 5373 2347
Size: 201 2329 496 2648
Fragments arranged by size:
2648 2329 496 201
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2319 1163
Size: 573 583 4518
Fragments arranged by size:
4518 583 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3124 3885 4913 1540
Size: 275 186 1123 761 1028 2301
Fragments arranged by size:
2301 1123 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3735 3820 4891 3735
Size: 85 1071 4518
Fragments arranged by size:
4518 1071 85
BsmI GAATG_Cn'
Cuts at: 3185 3278 3185
Size: 93 5581
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2993 3735 3820 4891 1391
Size: 399 1203 742 85 1071 2174
Fragments arranged by size:
2174 1203 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5337 5337
Size: 5674
BspHI T'CATG_A
Cuts at: 2139 2409 3563 5293 2139
Size: 270 1154 1730 2520
Fragments arranged by size:
2520 1730 1154 270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3029 844
Size: 2185 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3561 1394
Size: 429 1738 3507
Fragments arranged by size:
3507 1738 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4169 4343 65
Size: 1183 2921 174 1396
Fragments arranged by size:
2921 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2703 4318 4505 1700
Size: 110 893 1615 187 2869
Fragments arranged by size:
2869 1615 893 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5674
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5674
BssSI C'ACGA_G
Cuts at: 3734 4746 3734
Size: 1012 4662
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2424 794
Size: 1630 4044
BstBI TT'CG_AA
Cuts at: 4478 4478
Size: 5674
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5674
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2113 2011
Size: 102 5572
BstYI r'GATC_y
Cuts at: 1972 2513 2544 3004 3371 3756 3773 5225 5311
Size: 541 31 460 367 385 17 1452 86
Cuts at: 5311 5323 5669 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 541 460 385 367 346 86
31 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4183
Fragments arranged by size:
4183 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4935
Fragments arranged by size:
4935 683 56
BtsI GCAGTG_nn'
Cuts at: 3190 3984 4004 3190
Size: 794 20 4860
Fragments arranged by size:
4860 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5639
DraI TTT'AAA
Cuts at: 2978 2978
Size: 5674
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4681 809
Size: 507 3365 1802
Fragments arranged by size:
3365 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3121 4010 5639
Size: 54 1425 50 275 18 1291 889 1629
Cuts at: 5639 8
Size: 43
Fragments arranged by size:
1629 1425 1291 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3121 1537
Size: 275 1309 4090
Fragments arranged by size:
4090 1309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3604 1353
Size: 2251 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4261 4635 4781 387
Size: 1064 138 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2009 2237 2579 2790 3737 4334 5121
Size: 393 377 228 342 211 947 597 787
Cuts at: 5121 5389 1239
Size: 268 1524
Fragments arranged by size:
1524 947 787 597 393 377 342 268
228 211
EcoNI CCTnn'n_nnAGG
Cuts at: 2267 2267
Size: 5674
EcoO109I rG'GnC_Cy
Cuts at: 2257 2257
Size: 5674
EcoRI G'AATT_C
Cuts at: 2336 2983 3361 2336
Size: 647 378 4649
Fragments arranged by size:
4649 647 378
FspI TGC'GCA
Cuts at: 4180 4180
Size: 5674
HaeII r_GCGC'y
Cuts at: 1678 4821 1678
Size: 3143 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2249 2281 4315 4347 4389
Size: 32 135 32 677 32 2034 32 42
Cuts at: 4389 4421 1373
Size: 32 2626
Fragments arranged by size:
2626 2034 677 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3018 3264 669
Size: 988 281 1080 246 3079
Fragments arranged by size:
3079 1080 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5674
HpaI GTT'AAC
Cuts at: 3264 3264
Size: 5674
KpnI G_GTAC'C
Cuts at: 2401 2999 2401
Size: 598 5076
MfeI C'AATT_G
Cuts at: 3273 3273
Size: 5674
MluI A'CGCG_T
Cuts at: 5550 5550
Size: 5674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4788 4972 1305
Size: 3483 184 2007
Fragments arranged by size:
3483 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3852
Fragments arranged by size:
3852 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2111 2156 2180 2648 2741 2780
Size: 648 843 102 45 24 468 93 39
Cuts at: 2780 2852 2948 3632 3991 4150 518
Size: 72 96 684 359 159 2042
Fragments arranged by size:
2042 843 684 648 468 359 159 102
96 93 72 45 39 24
MspA1I CmG'CkG
Cuts at: 1606 3090 3767 4915 5160 1606
Size: 1484 677 1148 245 2120
Fragments arranged by size:
2120 1484 1148 677 245
NaeI GCC'GGC
Cuts at: 2705 2705
Size: 5674
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4183
Fragments arranged by size:
4183 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5674
NgoMIV G'CCGG_C
Cuts at: 2703 2703
Size: 5674
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5674
NotI GC'GGCC_GC
Cuts at: 3121 3121
Size: 5674
NspI r_CATG'y
Cuts at: 1132 1229 3032 4577 1132
Size: 97 1803 1545 2229
Fragments arranged by size:
2229 1803 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5674
PciI A'CATG_T
Cuts at: 4573 4573
Size: 5674
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2740 1108
Size: 1632 4042
PfoI T'CCnGG_A
Cuts at: 2253 2253
Size: 5674
PmeI GTTT'AAAC
Cuts at: 2978 2978
Size: 5674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2914 2946 3743 3775 5256 5288 2914
Size: 32 797 32 1481 32 3300
Fragments arranged by size:
3300 1481 797 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2257 2257
Size: 5674
PshAI GACnn'nnGTC
Cuts at: 1613 2486 1613
Size: 873 4801
PsiI TTA'TAA
Cuts at: 3244 3244
Size: 5674
PstI C_TGCA'G
Cuts at: 830 2902 3026 830
Size: 2072 124 3478
Fragments arranged by size:
3478 2072 124
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5674
SalI G'TCGA_C
Cuts at: 1936 3016 1936
Size: 1080 4594
SbfI CC_TGCA'GG
Cuts at: 3026 3026
Size: 5674
ScaI AGT'ACT
Cuts at: 2916 3922 5634 2916
Size: 1006 1712 2956
Fragments arranged by size:
2956 1712 1006
SfcI C'TryA_G
Cuts at: 826 1045 2509 2872 2898 3022 4157 4838 5029
Size: 219 1464 363 26 124 1135 681 191
Cuts at: 5029 826
Size: 1471
Fragments arranged by size:
1471 1464 1135 681 363 219 191 124
26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5674
SmaI CCC'GGG
Cuts at: 1872 2080 3001 1872
Size: 208 921 4545
Fragments arranged by size:
4545 921 208
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2878 3778 4679 4941 820
Size: 197 926 935 900 901 262 1553
Fragments arranged by size:
1553 935 926 901 900 262 197
SnaBI TAC'GTA
Cuts at: 493 3465 493
Size: 2972 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5674
SphI G_CATG'C
Cuts at: 1132 3032 1132
Size: 1900 3774
SspI AAT'ATT
Cuts at: 5 52 3598 5
Size: 47 3546 2081
Fragments arranged by size:
3546 2081 47
StuI AGG'CCT
Cuts at: 1517 2055 1517
Size: 538 5136
StyI C'CwwG_G
Cuts at: 513 1065 2004 2268 2463 3113 513
Size: 552 939 264 195 650 3074
Fragments arranged by size:
3074 939 650 552 264 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2078 2194 3701 3718 3871 4056 4501
Size: 1255 498 116 1507 17 153 185 445
Cuts at: 4501 325
Size: 1498
Fragments arranged by size:
1507 1498 1255 498 445 185 153 116
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 2914 3920
Size: 274 80 33 51 1443 207 730 1006
Cuts at: 3920 5632 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1006 730 274 207 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2147 2179 5634 5666 2147
Size: 32 3455 32 2155
Fragments arranged by size:
3455 2155 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5674
XbaI T'CTAG_A
Cuts at: 3010 3010
Size: 5674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2113 2287 2113
Size: 174 5500
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5674
XmaI C'CCGG_G
Cuts at: 1870 2078 2999 1870
Size: 208 921 4545
Fragments arranged by size:
4545 921 208
XmnI GAAnn'nnTTC
Cuts at: 3803 3803
Size: 5674
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3482 276
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI