Restriction map for
pFN21AE0277
(Circular) (Six-base) MAPSORT of: pFN21AE0277.seq Check: 1207 from: 1 to: 5668
pFN21AE0277 5668 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3478 278
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3011 1937
Size: 1074 4594
Acc65I G'GTAC_C
Cuts at: 2989 2989
Size: 5668
AclI AA'CG_TT
Cuts at: 1563 3795 4168 1563
Size: 2232 373 3063
Fragments arranged by size:
3063 2232 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2016 3731 5115 5383 1632
Size: 384 1715 1384 268 1917
Fragments arranged by size:
1917 1715 1384 384 268
AflII C'TTAA_G
Cuts at: 820 1017 2696 820
Size: 197 1679 3792
Fragments arranged by size:
3792 1679 197
AflIII A'CryG_T
Cuts at: 1203 4567 5544 1203
Size: 3364 977 1327
Fragments arranged by size:
3364 1327 977
AgeI A'CCGG_T
Cuts at: 2394 4499 2394
Size: 2105 3563
AhdI GACnn_n'nnGTC
Cuts at: 4397 4397
Size: 5668
AleI CACnn'nnGTG
Cuts at: 1166 2766 2810 1166
Size: 1600 44 4024
Fragments arranged by size:
4024 1600 44
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5668
AlwNI CAG_nnn'CTG
Cuts at: 3411 4983 3411
Size: 1572 4096
ApaLI G'TGCA_C
Cuts at: 2813 3725 4881 2813
Size: 912 1156 3600
Fragments arranged by size:
3600 1156 912
ApoI r'AATT_y
Cuts at: 1493 2056 2522 2977 3203 3355 5464 1493
Size: 563 466 455 226 152 2109 1697
Fragments arranged by size:
2109 1697 563 466 455 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4222 160
Size: 872 3190 1606
Fragments arranged by size:
3190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
AvaI C'yCGr_G
Cuts at: 1870 1943 2993 1870
Size: 73 1050 4545
Fragments arranged by size:
4545 1050 73
BamHI G'GATC_C
Cuts at: 2280 2998 3365 2280
Size: 718 367 4583
Fragments arranged by size:
4583 718 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2989 4444 618
Size: 325 217 627 1202 1455 1842
Fragments arranged by size:
1842 1455 1202 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2469 2987 1391
Size: 1078 518 4072
Fragments arranged by size:
4072 1078 518
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4697
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3859 3893 1903
Size: 34 1922 34 3678
Fragments arranged by size:
3678 1922 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3560 4770 3560
Size: 1210 4458
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5668
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4279 136
Size: 107 122 71 631 605 2607 1525
Fragments arranged by size:
2607 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5663 5663
Size: 5668
BlpI GC'TnA_GC
Cuts at: 2590 3085 2590
Size: 495 5173
Bme1580I G_kGCm'C
Cuts at: 1790 2817 3729 4885 1790
Size: 1027 912 1156 2573
Fragments arranged by size:
2573 1156 1027 912
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5668
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2323 3399 4357
Size: 416 53 83 399 24 906 1076 958
Cuts at: 4357 442
Size: 1753
Fragments arranged by size:
1753 1076 958 906 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4328 1239
Size: 3089 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3793 4658 4956 3793
Size: 865 298 4505
Fragments arranged by size:
4505 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4331 882
Size: 617 2832 2219
Fragments arranged by size:
2832 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3459 493
Size: 711 2255 2702
Fragments arranged by size:
2702 2255 711
BsaBI GATnn'nnATC
Cuts at: 5662 5662
Size: 5668
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3475 3857 4492 275
Size: 53 83 186 936 1942 382 635 1451
Fragments arranged by size:
1942 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2394 4101 4499 4773 4920 5331 2394
Size: 1707 398 274 147 411 2731
Fragments arranged by size:
2731 1707 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5443
Fragments arranged by size:
5443 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2116 2366 1186
Size: 930 250 4488
Fragments arranged by size:
4488 930 250
BseYI C'CCAG_C
Cuts at: 2275 4871 5367 2275
Size: 2596 496 2576
Fragments arranged by size:
2596 2576 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2180 2555 1163
Size: 573 444 375 4276
Fragments arranged by size:
4276 573 444 375
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3118 3879 4907 1540
Size: 275 186 1117 761 1028 2301
Fragments arranged by size:
2301 1117 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2817 3729 3814 4885 2817
Size: 912 85 1071 3600
Fragments arranged by size:
3600 1071 912 85
BsmI GAATG_Cn'
Cuts at: 2943 3179 3272 2943
Size: 236 93 5339
Fragments arranged by size:
5339 236 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2469 2817 2987 3729 3814 4885 1391
Size: 399 679 348 170 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 679 399 348 170 85
BspEI T'CCGG_A
Cuts at: 5331 5331
Size: 5668
BspHI T'CATG_A
Cuts at: 3557 5287 3557
Size: 1730 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2759 3023 844
Size: 1915 264 3489
Fragments arranged by size:
3489 1915 264
BsrBI CCG'CTC
Cuts at: 1394 1823 3555 1394
Size: 429 1732 3507
Fragments arranged by size:
3507 1732 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2322 2400 4163 4337 65
Size: 1183 1074 78 1763 174 1396
Fragments arranged by size:
1763 1396 1183 1074 174 78
BsrFI r'CCGG_y
Cuts at: 1700 1810 2394 4312 4499 1700
Size: 110 584 1918 187 2869
Fragments arranged by size:
2869 1918 584 187 110
BsrGI T'GTAC_A
Cuts at: 96 2265 96
Size: 2169 3499
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5668
BssSI C'ACGA_G
Cuts at: 3728 4740 3728
Size: 1012 4656
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5668
BstBI TT'CG_AA
Cuts at: 4472 4472
Size: 5668
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5668
BstYI r'GATC_y
Cuts at: 1972 2280 2578 2998 3365 3750 3767 5219 5305
Size: 308 298 420 367 385 17 1452 86
Cuts at: 5305 5317 5663 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 420 385 367 346 308 298
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2139 2344 2841 513
Size: 552 1074 205 497 3340
Fragments arranged by size:
3340 1074 552 497 205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4929
Fragments arranged by size:
4929 683 56
BtsI GCAGTG_nn'
Cuts at: 3184 3978 3998 3184
Size: 794 20 4854
Fragments arranged by size:
4854 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5633
DraI TTT'AAA
Cuts at: 2972 2972
Size: 5668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4675 809
Size: 507 3359 1802
Fragments arranged by size:
3359 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3115 4004 5633
Size: 54 1425 50 275 18 1285 889 1629
Cuts at: 5633 8
Size: 43
Fragments arranged by size:
1629 1425 1285 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3115 1537
Size: 275 1303 4090
Fragments arranged by size:
4090 1303 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2721 3598 1353
Size: 1368 877 3423
Fragments arranged by size:
3423 1368 877
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4255 4629 4775 387
Size: 1064 138 2666 374 146 1280
Fragments arranged by size:
2666 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2016 3731 4328 5115 5383 1239
Size: 393 384 1715 597 787 268 1524
Fragments arranged by size:
1715 1524 787 597 393 384 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2573 2573
Size: 5668
EcoRI G'AATT_C
Cuts at: 2056 2977 3355 2056
Size: 921 378 4369
Fragments arranged by size:
4369 921 378
FspI TGC'GCA
Cuts at: 4174 4174
Size: 5668
HaeII r_GCGC'y
Cuts at: 1678 4815 1678
Size: 3137 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4309 4341 4383 4415 1373
Size: 32 135 32 2737 32 42 32 2626
Fragments arranged by size:
2737 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3012 3258 669
Size: 988 281 1074 246 3079
Fragments arranged by size:
3079 1074 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5668
HpaI GTT'AAC
Cuts at: 3258 3258
Size: 5668
KpnI G_GTAC'C
Cuts at: 2993 2993
Size: 5668
MfeI C'AATT_G
Cuts at: 2155 3267 2155
Size: 1112 4556
MluI A'CGCG_T
Cuts at: 5544 5544
Size: 5668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2637 4782 4966 1305
Size: 1332 2145 184 2007
Fragments arranged by size:
2145 2007 1332 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3846
Fragments arranged by size:
3846 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2766 2810 3626 3985 4144 518
Size: 648 1600 44 816 359 159 2042
Fragments arranged by size:
2042 1600 816 648 359 159 44
MspA1I CmG'CkG
Cuts at: 1606 3084 3761 4909 5154 1606
Size: 1478 677 1148 245 2120
Fragments arranged by size:
2120 1478 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2139 2344 513
Size: 552 1074 205 3837
Fragments arranged by size:
3837 1074 552 205
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5668
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5668
NotI GC'GGCC_GC
Cuts at: 3115 3115
Size: 5668
NspI r_CATG'y
Cuts at: 1132 1229 3026 4571 1132
Size: 97 1797 1545 2229
Fragments arranged by size:
2229 1797 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5668
PciI A'CATG_T
Cuts at: 4567 4567
Size: 5668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5668
PmeI GTTT'AAAC
Cuts at: 2972 2972
Size: 5668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 5250 5282 3737
Size: 32 1481 32 4123
Fragments arranged by size:
4123 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5668
PsiI TTA'TAA
Cuts at: 3238 3238
Size: 5668
PstI C_TGCA'G
Cuts at: 830 3020 830
Size: 2190 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5668
SalI G'TCGA_C
Cuts at: 1936 3010 1936
Size: 1074 4594
SbfI CC_TGCA'GG
Cuts at: 3020 3020
Size: 5668
ScaI AGT'ACT
Cuts at: 3916 5628 3916
Size: 1712 3956
SfcI C'TryA_G
Cuts at: 826 1045 2905 3016 4151 4832 5023 826
Size: 219 1860 111 1135 681 191 1471
Fragments arranged by size:
1860 1471 1135 681 219 191 111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5668
SmaI CCC'GGG
Cuts at: 1872 2995 1872
Size: 1123 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2696 3772 4673 4935 820
Size: 197 926 753 1076 901 262 1553
Fragments arranged by size:
1553 1076 926 901 753 262 197
SnaBI TAC'GTA
Cuts at: 493 3459 493
Size: 2966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5668
SphI G_CATG'C
Cuts at: 1132 3026 1132
Size: 1894 3774
SspI AAT'ATT
Cuts at: 5 52 3592 5
Size: 47 3540 2081
Fragments arranged by size:
3540 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5668
StyI C'CwwG_G
Cuts at: 513 1065 2139 2344 3107 513
Size: 552 1074 205 763 3074
Fragments arranged by size:
3074 1074 763 552 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3695 3712 3865 4050 4495 325
Size: 1255 2115 17 153 185 445 1498
Fragments arranged by size:
2115 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2265 3914 5626
Size: 274 80 33 51 1443 288 1649 1712
Cuts at: 5626 96
Size: 138
Fragments arranged by size:
1712 1649 1443 288 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5628 5660 5628
Size: 32 5636
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5668
XbaI T'CTAG_A
Cuts at: 3004 3004
Size: 5668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2146 2855 2146
Size: 709 4959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5668
XmaI C'CCGG_G
Cuts at: 1870 2993 1870
Size: 1123 4545
XmnI GAAnn'nnTTC
Cuts at: 3797 3797
Size: 5668
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3476 276
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI