Restriction map for
pFN21AE0274
(Circular) (Six-base) MAPSORT of: pFN21AE0274.seq Check: 6151 from: 1 to: 5032
pFN21AE0274 5032 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2105 2842 278
Size: 53 83 186 936 569 737 2468
Fragments arranged by size:
2468 936 737 569 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2375 1937
Size: 438 4594
Acc65I G'GTAC_C
Cuts at: 2353 2353
Size: 5032
AclI AA'CG_TT
Cuts at: 1563 3159 3532 1563
Size: 1596 373 3063
Fragments arranged by size:
3063 1596 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3095 4479 4747 1632
Size: 1463 1384 268 1917
Fragments arranged by size:
1917 1463 1384 268
AfeI AGC'GCT
Cuts at: 2049 2049
Size: 5032
AflII C'TTAA_G
Cuts at: 820 1017 2195 820
Size: 197 1178 3657
Fragments arranged by size:
3657 1178 197
AflIII A'CryG_T
Cuts at: 1203 3931 4908 1203
Size: 2728 977 1327
Fragments arranged by size:
2728 1327 977
AgeI A'CCGG_T
Cuts at: 3863 3863
Size: 5032
AhdI GACnn_n'nnGTC
Cuts at: 3761 3761
Size: 5032
AleI CACnn'nnGTG
Cuts at: 1166 2018 1166
Size: 852 4180
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5032
AlwNI CAG_nnn'CTG
Cuts at: 2775 4347 2775
Size: 1572 3460
ApaLI G'TGCA_C
Cuts at: 3089 4245 3089
Size: 1156 3876
ApoI r'AATT_y
Cuts at: 1493 2090 2309 2341 2567 2719 4828 1493
Size: 597 219 32 226 152 2109 1697
Fragments arranged by size:
2109 1697 597 226 219 152 32
AseI AT'TA_AT
Cuts at: 160 1032 3586 160
Size: 872 2554 1606
Fragments arranged by size:
2554 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5032
AvaI C'yCGr_G
Cuts at: 1870 1943 2357 1870
Size: 73 414 4545
Fragments arranged by size:
4545 414 73
BamHI G'GATC_C
Cuts at: 2362 2729 2362
Size: 367 4665
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2225 2353 3808 618
Size: 325 217 627 438 128 1455 1842
Fragments arranged by size:
1842 1455 627 438 325 217 128
BanII G_rGCy'C
Cuts at: 1391 2351 1391
Size: 960 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4061
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3223 3257 1903
Size: 34 1286 34 3678
Fragments arranged by size:
3678 1286 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2924 4134 2924
Size: 1210 3822
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5032
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3643 136
Size: 107 122 71 631 605 1971 1525
Fragments arranged by size:
1971 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5027 5027
Size: 5032
BlpI GC'TnA_GC
Cuts at: 2449 2449
Size: 5032
Bme1580I G_kGCm'C
Cuts at: 1790 3093 4249 1790
Size: 1303 1156 2573
Fragments arranged by size:
2573 1303 1156
BmgBI CAC'GTC
Cuts at: 1318 2020 1318
Size: 702 4330
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2763 3721 442
Size: 416 53 83 399 24 1346 958 1753
Fragments arranged by size:
1753 1346 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5032
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2192 3692 1239
Size: 797 156 1500 2579
Fragments arranged by size:
2579 1500 797 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3157 4022 4320 3157
Size: 865 298 3869
Fragments arranged by size:
3869 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3695 882
Size: 617 2196 2219
Fragments arranged by size:
2219 2196 617
BsaAI yAC'GTr
Cuts at: 493 1204 2823 493
Size: 711 1619 2702
Fragments arranged by size:
2702 1619 711
BsaBI GATnn'nnATC
Cuts at: 5026 5026
Size: 5032
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2102 2839 3221 3856
Size: 53 83 186 936 569 737 382 635
Cuts at: 3856 275
Size: 1451
Fragments arranged by size:
1451 936 737 635 569 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2148 3465 3863 4137 4284 4695 2148
Size: 1317 398 274 147 411 2485
Fragments arranged by size:
2485 1317 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4807
Fragments arranged by size:
4807 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5032
BseYI C'CCAG_C
Cuts at: 4235 4731 4235
Size: 496 4536
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4459
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2482 3243 4271 1540
Size: 275 186 481 761 1028 2301
Fragments arranged by size:
2301 1028 761 481 275 186
BsiHKAI G_wGCw'C
Cuts at: 2025 3093 3178 4249 2025
Size: 1068 85 1071 2808
Fragments arranged by size:
2808 1071 1068 85
BsmI GAATG_Cn'
Cuts at: 2063 2326 2543 2636 2063
Size: 263 217 93 4459
Fragments arranged by size:
4459 263 217 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2351 3093 3178 4249 1391
Size: 399 235 326 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 326 235 85
BspEI T'CCGG_A
Cuts at: 4695 4695
Size: 5032
BspHI T'CATG_A
Cuts at: 2921 4651 2921
Size: 1730 3302
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2387 844
Size: 1543 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2172 2919 1394
Size: 429 349 747 3507
Fragments arranged by size:
3507 747 429 349
BsrDI GCAATG_nn'
Cuts at: 65 1248 2037 3527 3701 65
Size: 1183 789 1490 174 1396
Fragments arranged by size:
1490 1396 1183 789 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3676 3863 1700
Size: 110 1866 187 2869
Fragments arranged by size:
2869 1866 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5032
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5032
BssSI C'ACGA_G
Cuts at: 3092 4104 3092
Size: 1012 4020
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5032
BstBI TT'CG_AA
Cuts at: 3836 3836
Size: 5032
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5032
BstYI r'GATC_y
Cuts at: 1972 2362 2729 3114 3131 4583 4669 4681 5027
Size: 390 367 385 17 1452 86 12 346
Cuts at: 5027 1972
Size: 1977
Fragments arranged by size:
1977 1452 390 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4480
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4293
Fragments arranged by size:
4293 683 56
BtsI GCAGTG_nn'
Cuts at: 2548 3342 3362 2548
Size: 794 20 4218
Fragments arranged by size:
4218 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5032
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4997
DraI TTT'AAA
Cuts at: 2336 2336
Size: 5032
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4039 809
Size: 507 2723 1802
Fragments arranged by size:
2723 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2479 3368 4997
Size: 54 1425 50 275 18 649 889 1629
Cuts at: 4997 8
Size: 43
Fragments arranged by size:
1629 1425 889 649 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2479 1537
Size: 275 667 4090
Fragments arranged by size:
4090 667 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2184 2962 1353
Size: 831 778 3423
Fragments arranged by size:
3423 831 778
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2288 3619 3993 4139 387
Size: 1064 138 699 1331 374 146 1280
Fragments arranged by size:
1331 1280 1064 699 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2192 3095 3692 4479 4747 1239
Size: 393 404 156 903 597 787 268 1524
Fragments arranged by size:
1524 903 787 597 404 393 268 156
EcoRI G'AATT_C
Cuts at: 2309 2341 2719 2309
Size: 32 378 4622
Fragments arranged by size:
4622 378 32
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2210 2210
Size: 5032
FspI TGC'GCA
Cuts at: 3538 3538
Size: 5032
HaeII r_GCGC'y
Cuts at: 1678 2051 4179 1678
Size: 373 2128 2531
Fragments arranged by size:
2531 2128 373
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2161 2193 3673 3705 3747
Size: 32 135 32 589 32 1480 32 42
Cuts at: 3747 3779 1373
Size: 32 2626
Fragments arranged by size:
2626 1480 589 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2376 2622 669
Size: 988 281 438 246 3079
Fragments arranged by size:
3079 988 438 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5032
HpaI GTT'AAC
Cuts at: 2622 2622
Size: 5032
KpnI G_GTAC'C
Cuts at: 2357 2357
Size: 5032
MfeI C'AATT_G
Cuts at: 2631 2631
Size: 5032
MluI A'CGCG_T
Cuts at: 4908 4908
Size: 5032
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4146 4330 1305
Size: 2841 184 2007
Fragments arranged by size:
2841 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3210
Fragments arranged by size:
3210 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2018 2990 3349 3508 518
Size: 648 852 972 359 159 2042
Fragments arranged by size:
2042 972 852 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2448 3125 4273 4518 1606
Size: 842 677 1148 245 2120
Fragments arranged by size:
2120 1148 842 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4480
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5032
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5032
NotI GC'GGCC_GC
Cuts at: 2479 2479
Size: 5032
NspI r_CATG'y
Cuts at: 1132 1229 2390 3935 1132
Size: 97 1161 1545 2229
Fragments arranged by size:
2229 1545 1161 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5032
PciI A'CATG_T
Cuts at: 3931 3931
Size: 5032
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2320 1108
Size: 1212 3820
PfoI T'CCnGG_A
Cuts at: 2052 2313 2052
Size: 261 4771
PmeI GTTT'AAAC
Cuts at: 2336 2336
Size: 5032
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3101 3133 4614 4646 3101
Size: 32 1481 32 3487
Fragments arranged by size:
3487 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5032
PsiI TTA'TAA
Cuts at: 2602 2602
Size: 5032
PstI C_TGCA'G
Cuts at: 830 2384 830
Size: 1554 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5032
SalI G'TCGA_C
Cuts at: 1936 2374 1936
Size: 438 4594
SapI GCTCTTCn'nnn_
Cuts at: 2184 2184
Size: 5032
SbfI CC_TGCA'GG
Cuts at: 2384 2384
Size: 5032
ScaI AGT'ACT
Cuts at: 3280 4992 3280
Size: 1712 3320
SfcI C'TryA_G
Cuts at: 826 1045 2380 3515 4196 4387 826
Size: 219 1335 1135 681 191 1471
Fragments arranged by size:
1471 1335 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5032
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5032
SmaI CCC'GGG
Cuts at: 1872 2359 1872
Size: 487 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2195 3136 4037 4299 820
Size: 197 926 252 941 901 262 1553
Fragments arranged by size:
1553 941 926 901 262 252 197
SnaBI TAC'GTA
Cuts at: 493 2823 493
Size: 2330 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5032
SphI G_CATG'C
Cuts at: 1132 2390 1132
Size: 1258 3774
SspI AAT'ATT
Cuts at: 5 52 2956 5
Size: 47 2904 2081
Fragments arranged by size:
2904 2081 47
StuI AGG'CCT
Cuts at: 1517 2109 1517
Size: 592 4440
StyI C'CwwG_G
Cuts at: 513 1065 2471 513
Size: 552 1406 3074
Fragments arranged by size:
3074 1406 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2255 3059 3076 3229 3414 3859 325
Size: 1255 675 804 17 153 185 445 1498
Fragments arranged by size:
1498 1255 804 675 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3278 4990 96
Size: 274 80 33 51 1443 1301 1712 138
Fragments arranged by size:
1712 1443 1301 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4992 5024 4992
Size: 32 5000
Tth111I GACn'n_nGTC
Cuts at: 1316 2242 1316
Size: 926 4106
XbaI T'CTAG_A
Cuts at: 2368 2368
Size: 5032
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2260 2260
Size: 5032
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5032
XmaI C'CCGG_G
Cuts at: 1870 2357 1870
Size: 487 4545
XmnI GAAnn'nnTTC
Cuts at: 3161 3161
Size: 5032
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2103 2840 276
Size: 53 83 186 936 569 737 2468
Fragments arranged by size:
2468 936 737 569 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI