Restriction map for
pFN21AE0269
(Circular) (Six-base) MAPSORT of: pFN21AE0269.seq Check: 3424 from: 1 to: 6004
pFN21AE0269 6004 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3060 3060
Size: 6004
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3814 278
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3347 1937
Size: 1410 4594
Acc65I G'GTAC_C
Cuts at: 3325 3325
Size: 6004
AclI AA'CG_TT
Cuts at: 1563 4131 4504 1563
Size: 2568 373 3063
Fragments arranged by size:
3063 2568 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4067 5451 5719 1632
Size: 2435 1384 268 1917
Fragments arranged by size:
2435 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5807
AflIII A'CryG_T
Cuts at: 1203 4903 5880 1203
Size: 3700 977 1327
Fragments arranged by size:
3700 1327 977
AgeI A'CCGG_T
Cuts at: 4835 4835
Size: 6004
AhdI GACnn_n'nnGTC
Cuts at: 4733 4733
Size: 6004
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2522 2951 1184
Size: 1338 429 4237
Fragments arranged by size:
4237 1338 429
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2127 2159 2127
Size: 32 5972
AlwNI CAG_nnn'CTG
Cuts at: 2138 2519 2543 3022 3221 3747 5319 2138
Size: 381 24 479 199 526 1572 2823
Fragments arranged by size:
2823 1572 526 479 381 199 24
ApaI G_GGCC'C
Cuts at: 3185 3185
Size: 6004
ApaLI G'TGCA_C
Cuts at: 4061 5217 4061
Size: 1156 4848
ApoI r'AATT_y
Cuts at: 1493 2026 2785 3313 3539 3691 5800 1493
Size: 533 759 528 226 152 2109 1697
Fragments arranged by size:
2109 1697 759 533 528 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4558 160
Size: 872 3526 1606
Fragments arranged by size:
3526 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
AvaI C'yCGr_G
Cuts at: 1870 1943 3087 3286 3329 1870
Size: 73 1144 199 43 4545
Fragments arranged by size:
4545 1144 199 73 43
AvrII C'CTAG_G
Cuts at: 3173 3173
Size: 6004
BamHI G'GATC_C
Cuts at: 3107 3334 3701 3107
Size: 227 367 5410
Fragments arranged by size:
5410 367 227
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2873 3048 3325 4780 618
Size: 325 217 627 1086 175 277 1455 1842
Fragments arranged by size:
1842 1455 1086 627 325 277 217 175
BanII G_rGCy'C
Cuts at: 1391 3185 3323 1391
Size: 1794 138 4072
Fragments arranged by size:
4072 1794 138
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3298 928
Size: 971 1399 3634
Fragments arranged by size:
3634 1399 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4195 4229 1903
Size: 34 50 34 2174 34 3678
Fragments arranged by size:
3678 2174 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3896 5106 3896
Size: 1210 4794
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6004
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4615 136
Size: 107 122 71 631 605 2943 1525
Fragments arranged by size:
2943 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5999 5999
Size: 6004
BlpI GC'TnA_GC
Cuts at: 3421 3421
Size: 6004
Bme1580I G_kGCm'C
Cuts at: 1790 2852 3185 4065 5221 1790
Size: 1062 333 880 1156 2573
Fragments arranged by size:
2573 1156 1062 880 333
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6004
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2529 2627 3735
Size: 416 53 83 399 24 1112 98 1108
Cuts at: 3735 4693 442
Size: 958 1753
Fragments arranged by size:
1753 1112 1108 958 416 399 98 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6004
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2164 2519 2164
Size: 355 5649
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2975 3032 4664 1239
Size: 1736 57 1632 2579
Fragments arranged by size:
2579 1736 1632 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4129 4994 5292 4129
Size: 865 298 4841
Fragments arranged by size:
4841 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3138 4667 882
Size: 617 1639 1529 2219
Fragments arranged by size:
2219 1639 1529 617
BsaAI yAC'GTr
Cuts at: 493 1204 3795 493
Size: 711 2591 2702
Fragments arranged by size:
2702 2591 711
BsaBI GATnn'nnATC
Cuts at: 2283 5998 2283
Size: 3715 2289
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3811 4193 4828 275
Size: 53 83 186 936 2278 382 635 1451
Fragments arranged by size:
2278 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4437 4835 5109 5256 5667 4437
Size: 398 274 147 411 4774
Fragments arranged by size:
4774 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2100 2130 1178
Size: 30 165 30 697 30 5052
Fragments arranged by size:
5052 697 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2309 1186
Size: 1123 4881
BseYI C'CCAG_C
Cuts at: 2894 3036 3178 5207 5703 2894
Size: 142 142 2029 496 3195
Fragments arranged by size:
3195 2029 496 142 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2852 1163
Size: 573 1116 4315
Fragments arranged by size:
4315 1116 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3454 4215 5243 1540
Size: 275 186 1453 761 1028 2301
Fragments arranged by size:
2301 1453 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2998 3168 4065 4150 5221 2998
Size: 170 897 85 1071 3781
Fragments arranged by size:
3781 1071 897 170 85
BsmI GAATG_Cn'
Cuts at: 3515 3608 3515
Size: 93 5911
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2852 2998 3168 3185 3323 4065 4150
Size: 399 1062 146 170 17 138 742 85
Cuts at: 4150 5221 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1062 742 399 170 146 138
85 17
BspEI T'CCGG_A
Cuts at: 5667 5667
Size: 6004
BspHI T'CATG_A
Cuts at: 2454 3893 5623 2454
Size: 1439 1730 2835
Fragments arranged by size:
2835 1730 1439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3060 3359 844
Size: 2216 299 3489
Fragments arranged by size:
3489 2216 299
BsrBI CCG'CTC
Cuts at: 1394 1823 3891 1394
Size: 429 2068 3507
Fragments arranged by size:
3507 2068 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4499 4673 65
Size: 1183 3251 174 1396
Fragments arranged by size:
3251 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3027 3045 4648 4835 1700
Size: 110 1217 18 1603 187 2869
Fragments arranged by size:
2869 1603 1217 187 110 18
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6004
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6004
BssSI C'ACGA_G
Cuts at: 2153 2396 4064 5076 2153
Size: 243 1668 1012 3081
Fragments arranged by size:
3081 1668 1012 243
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2543 794
Size: 1749 4255
BstBI TT'CG_AA
Cuts at: 2092 4808 2092
Size: 2716 3288
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6004
BstXI CCAn_nnnn'nTGG
Cuts at: 2422 2617 2422
Size: 195 5809
BstYI r'GATC_y
Cuts at: 1972 2728 3107 3334 3701 4086 4103 5555 5641
Size: 756 379 227 367 385 17 1452 86
Cuts at: 5641 5653 5999 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 756 385 379 367 346 227
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5452
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2269 487
Size: 683 56 1043 4222
Fragments arranged by size:
4222 1043 683 56
BtsI GCAGTG_nn'
Cuts at: 3520 4314 4334 3520
Size: 794 20 5190
Fragments arranged by size:
5190 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5969
DraI TTT'AAA
Cuts at: 3308 3308
Size: 6004
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5011 809
Size: 507 3695 1802
Fragments arranged by size:
3695 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2424 2748 2793
Size: 54 1425 50 275 18 594 324 45
Cuts at: 2793 2859 3238 3451 4340 5969 8
Size: 66 379 213 889 1629 43
Fragments arranged by size:
1629 1425 889 594 379 324 275 213
66 54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3451 1537
Size: 275 1639 4090
Fragments arranged by size:
4090 1639 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2238 2437 3934 1353
Size: 885 199 1497 3423
Fragments arranged by size:
3423 1497 885 199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4591 4965 5111 387
Size: 1064 138 3002 374 146 1280
Fragments arranged by size:
3002 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2975 3032 4067 4664 5451 5719 1239
Size: 393 1343 57 1035 597 787 268 1524
Fragments arranged by size:
1524 1343 1035 787 597 393 268 57
EcoO109I rG'GnC_Cy
Cuts at: 2621 3182 3283 2621
Size: 561 101 5342
Fragments arranged by size:
5342 561 101
EcoRI G'AATT_C
Cuts at: 3313 3691 3313
Size: 378 5626
FspI TGC'GCA
Cuts at: 4510 4510
Size: 6004
HaeII r_GCGC'y
Cuts at: 1678 5151 1678
Size: 3473 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2100 2132 4645 4677 4719
Size: 32 135 32 528 32 2513 32 42
Cuts at: 4719 4751 1373
Size: 32 2626
Fragments arranged by size:
2626 2513 528 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2196 2386 3348 3594 669
Size: 988 281 258 190 962 246 3079
Fragments arranged by size:
3079 988 962 281 258 246 190
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6004
HpaI GTT'AAC
Cuts at: 3594 3594
Size: 6004
KpnI G_GTAC'C
Cuts at: 3329 3329
Size: 6004
MfeI C'AATT_G
Cuts at: 3603 3603
Size: 6004
MluI A'CGCG_T
Cuts at: 5880 5880
Size: 6004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2153 2781 5118 5302 1305
Size: 848 628 2337 184 2007
Fragments arranged by size:
2337 2007 848 628 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2426 2750 2795 2861 3240
Size: 54 1425 343 594 324 45 66 379
Cuts at: 3240 10
Size: 2774
Fragments arranged by size:
2774 1425 594 379 343 324 66 54
45
MslI CAynn'nnrTG
Cuts at: 518 1166 2208 2774 3962 4321 4480 518
Size: 648 1042 566 1188 359 159 2042
Fragments arranged by size:
2042 1188 1042 648 566 359 159
MspA1I CmG'CkG
Cuts at: 1606 2543 3420 4097 5245 5490 1606
Size: 937 877 677 1148 245 2120
Fragments arranged by size:
2120 1148 937 877 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5452
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6004
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6004
NotI GC'GGCC_GC
Cuts at: 3451 3451
Size: 6004
NspI r_CATG'y
Cuts at: 1132 1229 3362 4907 1132
Size: 97 2133 1545 2229
Fragments arranged by size:
2229 2133 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6004
PciI A'CATG_T
Cuts at: 4903 4903
Size: 6004
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6004
PmeI GTTT'AAAC
Cuts at: 3308 3308
Size: 6004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4073 4105 5586 5618 4073
Size: 32 1481 32 4459
Fragments arranged by size:
4459 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6004
PsiI TTA'TAA
Cuts at: 3574 3574
Size: 6004
PspOMI G'GGCC_C
Cuts at: 3181 3181
Size: 6004
PstI C_TGCA'G
Cuts at: 830 2940 3356 830
Size: 2110 416 3478
Fragments arranged by size:
3478 2110 416
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6004
PvuII CAG'CTG
Cuts at: 2543 2543
Size: 6004
SalI G'TCGA_C
Cuts at: 1936 3346 1936
Size: 1410 4594
SapI GCTCTTCn'nnn_
Cuts at: 2238 2437 2238
Size: 199 5805
SbfI CC_TGCA'GG
Cuts at: 3356 3356
Size: 6004
ScaI AGT'ACT
Cuts at: 4252 5964 4252
Size: 1712 4292
SfcI C'TryA_G
Cuts at: 826 1045 2936 3352 4487 5168 5359 826
Size: 219 1891 416 1135 681 191 1471
Fragments arranged by size:
1891 1471 1135 681 416 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6004
SmaI CCC'GGG
Cuts at: 1872 3089 3288 3331 1872
Size: 1217 199 43 4545
Fragments arranged by size:
4545 1217 199 43
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4108 5009 5271 820
Size: 197 926 2165 901 262 1553
Fragments arranged by size:
2165 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3795 493
Size: 3302 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6004
SphI G_CATG'C
Cuts at: 1132 3362 1132
Size: 2230 3774
SspI AAT'ATT
Cuts at: 5 52 3928 5
Size: 47 3876 2081
Fragments arranged by size:
3876 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6004
StyI C'CwwG_G
Cuts at: 513 1065 2046 3173 3227 3443 513
Size: 552 981 1127 54 216 3074
Fragments arranged by size:
3074 1127 981 552 216 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2869 2876 3050 4031 4048 4201 4386
Size: 1255 1289 7 174 981 17 153 185
Cuts at: 4386 4831 325
Size: 445 1498
Fragments arranged by size:
1498 1289 1255 981 445 185 174 153
17 7
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4250 5962 96
Size: 274 80 33 51 1443 2273 1712 138
Fragments arranged by size:
2273 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3099 3131 5964 5996 3099
Size: 32 2833 32 3107
Fragments arranged by size:
3107 2833 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6004
XbaI T'CTAG_A
Cuts at: 3340 3340
Size: 6004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2416 2416
Size: 6004
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6004
XmaI C'CCGG_G
Cuts at: 1870 3087 3286 3329 1870
Size: 1217 199 43 4545
Fragments arranged by size:
4545 1217 199 43
XmnI GAAnn'nnTTC
Cuts at: 2587 4133 2587
Size: 1546 4458
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3812 276
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI