Restriction map for
pFN21AE0268
(Circular) (Six-base) MAPSORT of: pFN21AE0268.seq Check: 753 from: 1 to: 5365
pFN21AE0268 5365 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3175 278
Size: 53 83 186 936 1639 2468
Fragments arranged by size:
2468 1639 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2631 2708 1937
Size: 694 77 4594
Fragments arranged by size:
4594 694 77
Acc65I G'GTAC_C
Cuts at: 2686 2686
Size: 5365
AclI AA'CG_TT
Cuts at: 1563 3492 3865 1563
Size: 1929 373 3063
Fragments arranged by size:
3063 1929 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3428 4812 5080 1632
Size: 1796 1384 268 1917
Fragments arranged by size:
1917 1796 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5168
AflIII A'CryG_T
Cuts at: 1203 4264 5241 1203
Size: 3061 977 1327
Fragments arranged by size:
3061 1327 977
AgeI A'CCGG_T
Cuts at: 4196 4196
Size: 5365
AhdI GACnn_n'nnGTC
Cuts at: 4094 4094
Size: 5365
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5365
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2204 1184
Size: 1020 4345
AlwNI CAG_nnn'CTG
Cuts at: 3108 4680 3108
Size: 1572 3793
ApaI G_GGCC'C
Cuts at: 2042 2429 2042
Size: 387 4978
ApaLI G'TGCA_C
Cuts at: 3422 4578 3422
Size: 1156 4209
ApoI r'AATT_y
Cuts at: 1493 2674 2900 3052 5161 1493
Size: 1181 226 152 2109 1697
Fragments arranged by size:
2109 1697 1181 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3919 160
Size: 872 2887 1606
Fragments arranged by size:
2887 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5365
AvaI C'yCGr_G
Cuts at: 1870 1943 2234 2661 2690 1870
Size: 73 291 427 29 4545
Fragments arranged by size:
4545 427 291 73 29
BamHI G'GATC_C
Cuts at: 2695 3062 2695
Size: 367 4998
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2031 2315 2686 4141 618
Size: 325 217 627 244 284 371 1455 1842
Fragments arranged by size:
1842 1455 627 371 325 284 244 217
BanII G_rGCy'C
Cuts at: 1391 2042 2084 2361 2429 2535 2684 1391
Size: 651 42 277 68 106 149 4072
Fragments arranged by size:
4072 651 277 149 106 68 42
BbeI G_GCGC'C
Cuts at: 2035 2035
Size: 5365
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2412 928
Size: 971 513 3881
Fragments arranged by size:
3881 971 513
BbvCI CC'TCA_GC
Cuts at: 2570 2570
Size: 5365
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3556 3590 1903
Size: 34 1619 34 3678
Fragments arranged by size:
3678 1619 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3257 4467 3257
Size: 1210 4155
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5365
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2549 3976 136
Size: 107 122 71 631 605 877 1427 1525
Fragments arranged by size:
1525 1427 877 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2367 5360 2367
Size: 2993 2372
BlpI GC'TnA_GC
Cuts at: 2782 2782
Size: 5365
Bme1580I G_kGCm'C
Cuts at: 1790 2042 2429 3426 4582 1790
Size: 252 387 997 1156 2573
Fragments arranged by size:
2573 1156 997 387 252
BmgBI CAC'GTC
Cuts at: 1318 2605 1318
Size: 1287 4078
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3096 4054 442
Size: 416 53 83 399 24 1679 958 1753
Fragments arranged by size:
1753 1679 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2086 2582 4025 1239
Size: 847 496 1443 2579
Fragments arranged by size:
2579 1443 847 496
Bpu10I CC'TnA_GC
Cuts at: 2570 2570
Size: 5365
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2531 3490 4355 4653 2531
Size: 959 865 298 3243
Fragments arranged by size:
3243 959 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2274 4028 882
Size: 617 775 1754 2219
Fragments arranged by size:
2219 1754 775 617
BsaAI yAC'GTr
Cuts at: 493 1204 3156 493
Size: 711 1952 2702
Fragments arranged by size:
2702 1952 711
BsaBI GATnn'nnATC
Cuts at: 5359 5359
Size: 5365
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2032 2136 2481 3172
Size: 53 83 186 936 499 104 345 691
Cuts at: 3172 3554 4189 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 691 635 499 382 345 186
104 83 53
BsaWI w'CCGG_w
Cuts at: 3798 4196 4470 4617 5028 3798
Size: 398 274 147 411 4135
Fragments arranged by size:
4135 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5140
Fragments arranged by size:
5140 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2252 1186
Size: 1066 4299
BseYI C'CCAG_C
Cuts at: 2090 2096 2195 2311 2557 4568 5064 2090
Size: 6 99 116 246 2011 496 2391
Fragments arranged by size:
2391 2011 496 246 116 99 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4792
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2221 2515 2815 3576 4604 1540
Size: 275 186 220 294 300 761 1028 2301
Fragments arranged by size:
2301 1028 761 300 294 275 220 186
BsiHKAI G_wGCw'C
Cuts at: 2157 2361 2535 3426 3511 4582 2157
Size: 204 174 891 85 1071 2940
Fragments arranged by size:
2940 1071 891 204 174 85
BsmI GAATG_Cn'
Cuts at: 2876 2969 2876
Size: 93 5272
BsmBI CGTCTCn'nnnn_
Cuts at: 2438 2438
Size: 5365
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2084 2157 2361 2429 2535 2684
Size: 399 252 42 73 204 68 106 149
Cuts at: 2684 3426 3511 4582 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 252 204 149 106
85 73 68 42
BspEI T'CCGG_A
Cuts at: 5028 5028
Size: 5365
BspHI T'CATG_A
Cuts at: 3254 4984 3254
Size: 1730 3635
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2245 2720 844
Size: 1401 475 3489
Fragments arranged by size:
3489 1401 475
BsrBI CCG'CTC
Cuts at: 1394 1823 3252 1394
Size: 429 1429 3507
Fragments arranged by size:
3507 1429 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2199 3860 4034 65
Size: 1183 951 1661 174 1396
Fragments arranged by size:
1661 1396 1183 951 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4009 4196 1700
Size: 110 2199 187 2869
Fragments arranged by size:
2869 2199 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5365
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5365
BssSI C'ACGA_G
Cuts at: 3425 4437 3425
Size: 1012 4353
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5365
BstBI TT'CG_AA
Cuts at: 2637 4169 2637
Size: 1532 3833
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5365
BstXI CCAn_nnnn'nTGG
Cuts at: 2353 2437 2353
Size: 84 5281
BstYI r'GATC_y
Cuts at: 1972 2367 2592 2695 3062 3447 3464 4916 5002
Size: 395 225 103 367 385 17 1452 86
Cuts at: 5002 5014 5360 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 395 385 367 346 225 103
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2043 2043
Size: 5365
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3874
Fragments arranged by size:
3874 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4626
Fragments arranged by size:
4626 683 56
BtsI GCAGTG_nn'
Cuts at: 2063 2881 3675 3695 2063
Size: 818 794 20 3733
Fragments arranged by size:
3733 818 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5365
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5330
DraI TTT'AAA
Cuts at: 2669 2669
Size: 5365
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2627 4372 809
Size: 507 1311 1745 1802
Fragments arranged by size:
1802 1745 1311 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2512 2812 3701
Size: 54 1425 50 275 18 682 300 889
Cuts at: 3701 5330 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 682 300 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2512 2812 1537
Size: 275 700 300 4090
Fragments arranged by size:
4090 700 300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2241 2634 3295 1353
Size: 888 393 661 3423
Fragments arranged by size:
3423 888 661 393
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2403 3952 4326 4472 387
Size: 1064 138 814 1549 374 146 1280
Fragments arranged by size:
1549 1280 1064 814 374 146 138
EcoICRI GAG'CTC
Cuts at: 2359 2533 2359
Size: 174 5191
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2086 2582 3428 4025 4812 5080 1239
Size: 393 454 496 846 597 787 268 1524
Fragments arranged by size:
1524 846 787 597 496 454 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2038 2039 2047 2122 2171 2426 2551 2038
Size: 1 8 75 49 255 125 4852
Fragments arranged by size:
4852 255 125 75 49 8 1
EcoRI G'AATT_C
Cuts at: 2674 3052 2674
Size: 378 4987
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2416 2068
Size: 348 5017
FspI TGC'GCA
Cuts at: 3871 3871
Size: 5365
HaeII r_GCGC'y
Cuts at: 1678 2035 2569 4512 1678
Size: 357 534 1943 2531
Fragments arranged by size:
2531 1943 534 357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4006 4038 4080 4112 1373
Size: 32 135 32 2434 32 42 32 2626
Fragments arranged by size:
2626 2434 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2632 2709 2955 669
Size: 988 281 694 77 246 3079
Fragments arranged by size:
3079 988 694 281 246 77
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5365
HpaI GTT'AAC
Cuts at: 2955 2955
Size: 5365
KasI G'GCGC_C
Cuts at: 2031 2031
Size: 5365
KpnI G_GTAC'C
Cuts at: 2690 2690
Size: 5365
MfeI C'AATT_G
Cuts at: 2964 2964
Size: 5365
MluI A'CGCG_T
Cuts at: 5241 5241
Size: 5365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4479 4663 1305
Size: 3174 184 2007
Fragments arranged by size:
3174 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3543
Fragments arranged by size:
3543 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 2522 3323 3682 3841 518
Size: 648 1185 171 801 359 159 2042
Fragments arranged by size:
2042 1185 801 648 359 171 159
MspA1I CmG'CkG
Cuts at: 1606 2578 2781 3458 4606 4851 1606
Size: 972 203 677 1148 245 2120
Fragments arranged by size:
2120 1148 972 677 245 203
NarI GG'CG_CC
Cuts at: 2032 2032
Size: 5365
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3874
Fragments arranged by size:
3874 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5365
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5365
NotI GC'GGCC_GC
Cuts at: 2812 2812
Size: 5365
NspI r_CATG'y
Cuts at: 1132 1229 2326 2344 2723 4268 1132
Size: 97 1097 18 379 1545 2229
Fragments arranged by size:
2229 1545 1097 379 97 18
PasI CC'CwG_GG
Cuts at: 1348 2332 1348
Size: 984 4381
PciI A'CATG_T
Cuts at: 4264 4264
Size: 5365
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2605 1108
Size: 1497 3868
PfoI T'CCnGG_A
Cuts at: 2265 2466 2487 2598 2265
Size: 201 21 111 5032
Fragments arranged by size:
5032 201 111 21
PmeI GTTT'AAAC
Cuts at: 2669 2669
Size: 5365
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3434 3466 4947 4979 3434
Size: 32 1481 32 3820
Fragments arranged by size:
3820 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2047 2122 2047
Size: 75 5290
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5365
PsiI TTA'TAA
Cuts at: 2935 2935
Size: 5365
PspOMI G'GGCC_C
Cuts at: 2038 2425 2038
Size: 387 4978
PstI C_TGCA'G
Cuts at: 830 2256 2717 830
Size: 1426 461 3478
Fragments arranged by size:
3478 1426 461
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5365
SacI G_AGCT'C
Cuts at: 2361 2535 2361
Size: 174 5191
SalI G'TCGA_C
Cuts at: 1936 2630 2707 1936
Size: 694 77 4594
Fragments arranged by size:
4594 694 77
SanDI GG'GwC_CC
Cuts at: 2122 2122
Size: 5365
SapI GCTCTTCn'nnn_
Cuts at: 2241 2241
Size: 5365
SbfI CC_TGCA'GG
Cuts at: 2717 2717
Size: 5365
ScaI AGT'ACT
Cuts at: 3613 5325 3613
Size: 1712 3653
SfcI C'TryA_G
Cuts at: 826 1045 2252 2713 3848 4529 4720 826
Size: 219 1207 461 1135 681 191 1471
Fragments arranged by size:
1471 1207 1135 681 461 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2549 2549
Size: 5365
SfoI GGC'GCC
Cuts at: 2033 2033
Size: 5365
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5365
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5365
SmaI CCC'GGG
Cuts at: 1872 2692 1872
Size: 820 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2546 3469 4370 4632 820
Size: 197 926 603 923 901 262 1553
Fragments arranged by size:
1553 926 923 901 603 262 197
SnaBI TAC'GTA
Cuts at: 493 3156 493
Size: 2663 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5365
SphI G_CATG'C
Cuts at: 1132 2326 2723 1132
Size: 1194 397 3774
Fragments arranged by size:
3774 1194 397
SspI AAT'ATT
Cuts at: 5 52 3289 5
Size: 47 3237 2081
Fragments arranged by size:
3237 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5365
StyI C'CwwG_G
Cuts at: 513 1065 2004 2075 2109 2804 513
Size: 552 939 71 34 695 3074
Fragments arranged by size:
3074 939 695 552 71 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2225 2292 2303 3392 3409 3562 3747
Size: 1255 645 67 11 1089 17 153 185
Cuts at: 3747 4192 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1089 645 445 185 153 67
17 11
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3611 5323 96
Size: 274 80 33 51 1443 1634 1712 138
Fragments arranged by size:
1712 1634 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5325 5357 5325
Size: 32 5333
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5365
XbaI T'CTAG_A
Cuts at: 2701 2701
Size: 5365
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5365
XmaI C'CCGG_G
Cuts at: 1870 2690 1870
Size: 820 4545
XmnI GAAnn'nnTTC
Cuts at: 3494 3494
Size: 5365
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3173 276
Size: 53 83 186 936 1639 2468
Fragments arranged by size:
2468 1639 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII
RsrII SacII SexAI SrfI SwaI XcmI