Restriction map for
pFN21AE0254
(Circular) (Six-base) MAPSORT of: pFN21AE0254.seq Check: 876 from: 1 to: 5683
pFN21AE0254 5683 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 278
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2046 3026 1937
Size: 109 980 4594
Fragments arranged by size:
4594 980 109
Acc65I G'GTAC_C
Cuts at: 3004 3004
Size: 5683
AclI AA'CG_TT
Cuts at: 1563 3810 4183 1563
Size: 2247 373 3063
Fragments arranged by size:
3063 2247 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2756 3746 5130 5398 1632
Size: 1124 990 1384 268 1917
Fragments arranged by size:
1917 1384 1124 990 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5486
AflIII A'CryG_T
Cuts at: 1203 4582 5559 1203
Size: 3379 977 1327
Fragments arranged by size:
3379 1327 977
AgeI A'CCGG_T
Cuts at: 4514 4514
Size: 5683
AhdI GACnn_n'nnGTC
Cuts at: 4412 4412
Size: 5683
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5683
AlwNI CAG_nnn'CTG
Cuts at: 2531 2884 3426 4998 2531
Size: 353 542 1572 3216
Fragments arranged by size:
3216 1572 542 353
ApaLI G'TGCA_C
Cuts at: 3740 4896 3740
Size: 1156 4527
ApoI r'AATT_y
Cuts at: 1493 2015 2110 2992 3218 3370 5479 1493
Size: 522 95 882 226 152 2109 1697
Fragments arranged by size:
2109 1697 882 522 226 152 95
AseI AT'TA_AT
Cuts at: 160 1032 4237 160
Size: 872 3205 1606
Fragments arranged by size:
3205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
AvaI C'yCGr_G
Cuts at: 1870 1943 3008 1870
Size: 73 1065 4545
Fragments arranged by size:
4545 1065 73
BamHI G'GATC_C
Cuts at: 3013 3380 3013
Size: 367 5316
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3004 4459 618
Size: 325 217 627 1217 1455 1842
Fragments arranged by size:
1842 1455 1217 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2811 2927 3002 1391
Size: 1420 116 75 4072
Fragments arranged by size:
4072 1420 116 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4712
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3874 3908 1903
Size: 34 1937 34 3678
Fragments arranged by size:
3678 1937 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3575 4785 3575
Size: 1210 4473
BclI T'GATC_A
Cuts at: 1548 2149 2947 1548
Size: 601 798 4284
Fragments arranged by size:
4284 798 601
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4294 136
Size: 107 122 71 631 605 2622 1525
Fragments arranged by size:
2622 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2719 5678 2719
Size: 2959 2724
BlpI GC'TnA_GC
Cuts at: 3100 3100
Size: 5683
Bme1580I G_kGCm'C
Cuts at: 1790 3744 4900 1790
Size: 1954 1156 2573
Fragments arranged by size:
2573 1954 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5683
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3414 4372 442
Size: 416 53 83 399 24 1997 958 1753
Fragments arranged by size:
1997 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5683
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2941 2941
Size: 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2399 2906 4343 1239
Size: 1160 507 1437 2579
Fragments arranged by size:
2579 1437 1160 507
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2849 3808 4673 4971 2849
Size: 959 865 298 3561
Fragments arranged by size:
3561 959 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4346 882
Size: 617 2847 2219
Fragments arranged by size:
2847 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3474 493
Size: 711 2270 2702
Fragments arranged by size:
2702 2270 711
BsaBI GATnn'nnATC
Cuts at: 5677 5677
Size: 5683
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3490 3872 4507 275
Size: 53 83 186 936 1957 382 635 1451
Fragments arranged by size:
1957 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4116 4514 4788 4935 5346 4116
Size: 398 274 147 411 4453
Fragments arranged by size:
4453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2487 2517 2613 2643 1178
Size: 30 165 30 1084 30 96 30 4218
Fragments arranged by size:
4218 1084 165 96 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2402 1186
Size: 1216 4467
BseYI C'CCAG_C
Cuts at: 4886 5382 4886
Size: 496 5187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5110
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3133 3894 4922 1540
Size: 275 186 1132 761 1028 2301
Fragments arranged by size:
2301 1132 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2865 2927 3744 3829 4900 2865
Size: 62 817 85 1071 3648
Fragments arranged by size:
3648 1071 817 85 62
BsmI GAATG_Cn'
Cuts at: 2263 3194 3287 2263
Size: 931 93 4659
Fragments arranged by size:
4659 931 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2811 2865 2927 3002 3744 3829 4900
Size: 399 1021 54 62 75 742 85 1071
Cuts at: 4900 1391
Size: 2174
Fragments arranged by size:
2174 1071 1021 742 399 85 75 62
54
BspEI T'CCGG_A
Cuts at: 5346 5346
Size: 5683
BspHI T'CATG_A
Cuts at: 2604 3572 5302 2604
Size: 968 1730 2985
Fragments arranged by size:
2985 1730 968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3038 844
Size: 2194 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3570 1394
Size: 429 1747 3507
Fragments arranged by size:
3507 1747 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2301 2965 4178 4352 65
Size: 1183 1053 664 1213 174 1396
Fragments arranged by size:
1396 1213 1183 1053 664 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4327 4514 1700
Size: 110 2517 187 2869
Fragments arranged by size:
2869 2517 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5683
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5683
BssSI C'ACGA_G
Cuts at: 3743 4755 3743
Size: 1012 4671
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2531 794
Size: 1737 3946
BstBI TT'CG_AA
Cuts at: 4487 4487
Size: 5683
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5683
BstXI CCAn_nnnn'nTGG
Cuts at: 2032 2296 2032
Size: 264 5419
BstYI r'GATC_y
Cuts at: 1972 2247 2656 2719 3013 3380 3765 3782 5234
Size: 275 409 63 294 367 385 17 1452
Cuts at: 5234 5320 5332 5678 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 409 385 367 346 294 275
86 63 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2624 513
Size: 552 1559 3572
Fragments arranged by size:
3572 1559 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4944
Fragments arranged by size:
4944 683 56
BtsI GCAGTG_nn'
Cuts at: 3199 3993 4013 3199
Size: 794 20 4869
Fragments arranged by size:
4869 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5648
DraI TTT'AAA
Cuts at: 2587 2987 2587
Size: 400 5283
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4690 809
Size: 507 3374 1802
Fragments arranged by size:
3374 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2286 3130 4019
Size: 54 1425 50 275 18 456 844 889
Cuts at: 4019 5648 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 844 456 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3130 1537
Size: 275 1318 4090
Fragments arranged by size:
4090 1318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2111 2156 3613 1353
Size: 758 45 1457 3423
Fragments arranged by size:
3423 1457 758 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4270 4644 4790 387
Size: 1064 138 2681 374 146 1280
Fragments arranged by size:
2681 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2925 2925
Size: 5683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2399 2756 2906 3746 4343 5130 5398
Size: 393 767 357 150 840 597 787 268
Cuts at: 5398 1239
Size: 1524
Fragments arranged by size:
1524 840 787 767 597 393 357 268
150
EcoRI G'AATT_C
Cuts at: 2992 3370 2992
Size: 378 5305
FspI TGC'GCA
Cuts at: 4189 4189
Size: 5683
HaeII r_GCGC'y
Cuts at: 1678 4830 1678
Size: 3152 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2711 2743 4324 4356 4398
Size: 32 135 32 1139 32 1581 32 42
Cuts at: 4398 4430 1373
Size: 32 2626
Fragments arranged by size:
2626 1581 1139 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3027 3273 669
Size: 988 281 1089 246 3079
Fragments arranged by size:
3079 1089 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5683
HpaI GTT'AAC
Cuts at: 3273 3273
Size: 5683
KpnI G_GTAC'C
Cuts at: 3008 3008
Size: 5683
MfeI C'AATT_G
Cuts at: 3282 3282
Size: 5683
MluI A'CGCG_T
Cuts at: 5559 5559
Size: 5683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2452 4797 4981 1305
Size: 1147 2345 184 2007
Fragments arranged by size:
2345 2007 1147 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2288 10
Size: 54 1425 343 456 3405
Fragments arranged by size:
3405 1425 456 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3641 4000 4159 518
Size: 648 2475 359 159 2042
Fragments arranged by size:
2475 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2945 3099 3776 4924 5169 1606
Size: 1339 154 677 1148 245 2120
Fragments arranged by size:
2120 1339 1148 677 245 154
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5131
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5683
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5683
NotI GC'GGCC_GC
Cuts at: 3130 3130
Size: 5683
NspI r_CATG'y
Cuts at: 1132 1229 2053 3041 4586 1132
Size: 97 824 988 1545 2229
Fragments arranged by size:
2229 1545 988 824 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5683
PciI A'CATG_T
Cuts at: 4582 4582
Size: 5683
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5683
PmeI GTTT'AAAC
Cuts at: 2987 2987
Size: 5683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 5265 5297 3752
Size: 32 1481 32 4138
Fragments arranged by size:
4138 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5683
PsiI TTA'TAA
Cuts at: 2234 3253 2234
Size: 1019 4664
PstI C_TGCA'G
Cuts at: 830 2256 3035 830
Size: 1426 779 3478
Fragments arranged by size:
3478 1426 779
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5683
PvuII CAG'CTG
Cuts at: 2945 2945
Size: 5683
SacI G_AGCT'C
Cuts at: 2927 2927
Size: 5683
SalI G'TCGA_C
Cuts at: 1936 3025 1936
Size: 1089 4594
SbfI CC_TGCA'GG
Cuts at: 3035 3035
Size: 5683
ScaI AGT'ACT
Cuts at: 3931 5643 3931
Size: 1712 3971
SfcI C'TryA_G
Cuts at: 826 1045 2064 2252 3031 4166 4847 5038 826
Size: 219 1019 188 779 1135 681 191 1471
Fragments arranged by size:
1471 1135 1019 779 681 219 191 188
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5683
SmaI CCC'GGG
Cuts at: 1872 3010 1872
Size: 1138 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2864 3787 4688 4950 820
Size: 197 926 921 923 901 262 1553
Fragments arranged by size:
1553 926 923 921 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3474 493
Size: 2981 2702
SpeI A'CTAG_T
Cuts at: 152 2824 152
Size: 2672 3011
SphI G_CATG'C
Cuts at: 1132 3041 1132
Size: 1909 3774
SspI AAT'ATT
Cuts at: 5 52 2706 3607 5
Size: 47 2654 901 2081
Fragments arranged by size:
2654 2081 901 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5683
StyI C'CwwG_G
Cuts at: 513 1065 3122 513
Size: 552 2057 3074
Fragments arranged by size:
3074 2057 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3710 3727 3880 4065 4510 325
Size: 1255 2130 17 153 185 445 1498
Fragments arranged by size:
2130 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3929 5641 96
Size: 274 80 33 51 1443 1952 1712 138
Fragments arranged by size:
1952 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2613 2645 5643 5675 2613
Size: 32 2998 32 2621
Fragments arranged by size:
2998 2621 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5683
XbaI T'CTAG_A
Cuts at: 3019 3019
Size: 5683
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5683
XmaI C'CCGG_G
Cuts at: 1870 3008 1870
Size: 1138 4545
XmnI GAAnn'nnTTC
Cuts at: 2396 3812 2396
Size: 1416 4267
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 276
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI