Restriction map for
pFN21AE0243
(Circular) (Six-base) MAPSORT of: pFN21AE0243.seq Check: 1040 from: 1 to: 5869
pFN21AE0243 5869 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3679 278
Size: 53 83 186 936 2143 2468
Fragments arranged by size:
2468 2143 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2319 2649 3212 1937
Size: 382 330 563 4594
Fragments arranged by size:
4594 563 382 330
Acc65I G'GTAC_C
Cuts at: 3190 3190
Size: 5869
AclI AA'CG_TT
Cuts at: 1563 3996 4369 1563
Size: 2433 373 3063
Fragments arranged by size:
3063 2433 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2138 2150 3932 5316 5584 1632
Size: 506 12 1782 1384 268 1917
Fragments arranged by size:
1917 1782 1384 506 268 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5672
AflIII A'CryG_T
Cuts at: 1203 4768 5745 1203
Size: 3565 977 1327
Fragments arranged by size:
3565 1327 977
AgeI A'CCGG_T
Cuts at: 4700 4700
Size: 5869
AhdI GACnn_n'nnGTC
Cuts at: 4598 4598
Size: 5869
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5869
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2864 1184
Size: 1680 4189
AlwNI CAG_nnn'CTG
Cuts at: 2206 2623 3612 5184 2206
Size: 417 989 1572 2891
Fragments arranged by size:
2891 1572 989 417
ApaLI G'TGCA_C
Cuts at: 3926 5082 3926
Size: 1156 4713
ApoI r'AATT_y
Cuts at: 1493 3178 3404 3556 5665 1493
Size: 1685 226 152 2109 1697
Fragments arranged by size:
2109 1697 1685 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4423 160
Size: 872 3391 1606
Fragments arranged by size:
3391 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
AvaI C'yCGr_G
Cuts at: 1870 1943 3194 1870
Size: 73 1251 4545
Fragments arranged by size:
4545 1251 73
BamHI G'GATC_C
Cuts at: 3087 3199 3566 3087
Size: 112 367 5390
Fragments arranged by size:
5390 367 112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2308 2417 2734 2840 3190
Size: 325 217 627 521 109 317 106 350
Cuts at: 3190 4645 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 521 350 325 317 217
109 106
BanII G_rGCy'C
Cuts at: 1391 3188 1391
Size: 1797 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2959 3094 3118 928
Size: 971 1060 135 24 3679
Fragments arranged by size:
3679 1060 971 135 24
BbvCI CC'TCA_GC
Cuts at: 2654 2811 2654
Size: 157 5712
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4060 4094 1903
Size: 34 2123 34 3678
Fragments arranged by size:
3678 2123 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2438 3761 4971 2438
Size: 1323 1210 3336
Fragments arranged by size:
3336 1323 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5869
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3164 4480 136
Size: 107 122 71 631 605 1492 1316 1525
Fragments arranged by size:
1525 1492 1316 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2487 5864 2487
Size: 3377 2492
BlpI GC'TnA_GC
Cuts at: 2938 3286 2938
Size: 348 5521
Bme1580I G_kGCm'C
Cuts at: 1790 2313 3930 5086 1790
Size: 523 1617 1156 2573
Fragments arranged by size:
2573 1617 1156 523
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5869
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2164 2740 2997
Size: 416 53 83 399 24 747 576 257
Cuts at: 2997 3600 4558 442
Size: 603 958 1753
Fragments arranged by size:
1753 958 747 603 576 416 399 257
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5869
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 5869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2228 2311 3096 4529 1239
Size: 989 83 785 1433 2579
Fragments arranged by size:
2579 1433 989 785 83
Bpu10I CC'TnA_GC
Cuts at: 2498 2654 2811 2498
Size: 156 157 5556
Fragments arranged by size:
5556 157 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2107 3994 4859 5157 2107
Size: 1887 865 298 2819
Fragments arranged by size:
2819 1887 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4532 882
Size: 617 3033 2219
Fragments arranged by size:
3033 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3660 493
Size: 711 2456 2702
Fragments arranged by size:
2702 2456 711
BsaBI GATnn'nnATC
Cuts at: 5863 5863
Size: 5869
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3676 4058 4693 275
Size: 53 83 186 936 2143 382 635 1451
Fragments arranged by size:
2143 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4302 4700 4974 5121 5532 4302
Size: 398 274 147 411 4639
Fragments arranged by size:
4639 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2276 2306 1178
Size: 30 165 30 873 30 4741
Fragments arranged by size:
4741 873 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2284 2569 1186
Size: 1098 285 4486
Fragments arranged by size:
4486 1098 285
BseYI C'CCAG_C
Cuts at: 2023 2149 2567 5072 5568 2023
Size: 126 418 2505 496 2324
Fragments arranged by size:
2505 2324 496 418 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2323 1163
Size: 573 587 4709
Fragments arranged by size:
4709 587 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3319 4080 5108 1540
Size: 275 186 1318 761 1028 2301
Fragments arranged by size:
2301 1318 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3930 4015 5086 3930
Size: 85 1071 4713
Fragments arranged by size:
4713 1071 85
BsmI GAATG_Cn'
Cuts at: 3380 3473 3380
Size: 93 5776
BsmBI CGTCTCn'nnnn_
Cuts at: 2498 2498
Size: 5869
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2313 3188 3930 4015 5086 1391
Size: 399 523 875 742 85 1071 2174
Fragments arranged by size:
2174 1071 875 742 523 399 85
BspEI T'CCGG_A
Cuts at: 5532 5532
Size: 5869
BspHI T'CATG_A
Cuts at: 3758 5488 3758
Size: 1730 4139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3224 844
Size: 2380 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3756 1394
Size: 429 1933 3507
Fragments arranged by size:
3507 1933 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2871 4364 4538 65
Size: 1183 1623 1493 174 1396
Fragments arranged by size:
1623 1493 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2288 2420 2837 2855 4513 4700 1700
Size: 110 478 132 417 18 1658 187 2869
Fragments arranged by size:
2869 1658 478 417 187 132 110 18
BsrGI T'GTAC_A
Cuts at: 96 2055 96
Size: 1959 3910
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5869
BssSI C'ACGA_G
Cuts at: 3929 4941 3929
Size: 1012 4857
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5869
BstBI TT'CG_AA
Cuts at: 4673 4673
Size: 5869
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5869
BstYI r'GATC_y
Cuts at: 1972 2487 3087 3199 3566 3951 3968 5420 5506
Size: 515 600 112 367 385 17 1452 86
Cuts at: 5506 5518 5864 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 600 515 385 367 346 112
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2364 2364
Size: 5869
BtgI C'CryG_G
Cuts at: 513 1065 2769 3162 513
Size: 552 1704 393 3220
Fragments arranged by size:
3220 1704 552 393
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2785 487
Size: 683 56 1559 3571
Fragments arranged by size:
3571 1559 683 56
BtsI GCAGTG_nn'
Cuts at: 3385 4179 4199 3385
Size: 794 20 5055
Fragments arranged by size:
5055 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5869
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5834
DraI TTT'AAA
Cuts at: 3173 3173
Size: 5869
DraIII CAC_nnn'GTG
Cuts at: 3005 3005
Size: 5869
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2927 4876 809
Size: 507 1611 1949 1802
Fragments arranged by size:
1949 1802 1611 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3316 4205 5834
Size: 54 1425 50 275 18 1486 889 1629
Cuts at: 5834 8
Size: 43
Fragments arranged by size:
1629 1486 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3316 1537
Size: 275 1504 4090
Fragments arranged by size:
4090 1504 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2645 2710 3799 1353
Size: 1292 65 1089 3423
Fragments arranged by size:
3423 1292 1089 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3098 4456 4830 4976 387
Size: 1064 138 1509 1358 374 146 1280
Fragments arranged by size:
1509 1358 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2150 2228 2311 3096 3932 4529
Size: 393 506 12 78 83 785 836 597
Cuts at: 4529 5316 5584 1239
Size: 787 268 1524
Fragments arranged by size:
1524 836 787 785 597 506 393 268
83 78 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2117 2117
Size: 5869
EcoO109I rG'GnC_Cy
Cuts at: 2062 2620 2635 2062
Size: 558 15 5296
Fragments arranged by size:
5296 558 15
EcoRI G'AATT_C
Cuts at: 3178 3556 3178
Size: 378 5491
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 5869
FspI TGC'GCA
Cuts at: 4375 4375
Size: 5869
HaeII r_GCGC'y
Cuts at: 1678 2039 2661 5016 1678
Size: 361 622 2355 2531
Fragments arranged by size:
2531 2355 622 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2276 2308 2627 2659 2784
Size: 32 135 32 704 32 319 32 125
Cuts at: 2784 2816 4510 4542 4584 4616 1373
Size: 32 1694 32 42 32 2626
Fragments arranged by size:
2626 1694 704 319 135 125 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2833 3213 3459 669
Size: 988 281 895 380 246 3079
Fragments arranged by size:
3079 988 895 380 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5869
HpaI GTT'AAC
Cuts at: 3459 3459
Size: 5869
KpnI G_GTAC'C
Cuts at: 3194 3194
Size: 5869
MfeI C'AATT_G
Cuts at: 3468 3468
Size: 5869
MluI A'CGCG_T
Cuts at: 5745 5745
Size: 5869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2322 2443 4983 5167 1305
Size: 1017 121 2540 184 2007
Fragments arranged by size:
2540 2007 1017 184 121
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4047
Fragments arranged by size:
4047 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2783 3827 4186 4345 518
Size: 648 1617 1044 359 159 2042
Fragments arranged by size:
2042 1617 1044 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2934 3285 3962 5110 5355 1606
Size: 1328 351 677 1148 245 2120
Fragments arranged by size:
2120 1328 1148 677 351 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5317
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5869
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5869
NotI GC'GGCC_GC
Cuts at: 3316 3316
Size: 5869
NspI r_CATG'y
Cuts at: 1132 1229 3227 4772 1132
Size: 97 1998 1545 2229
Fragments arranged by size:
2229 1998 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5869
PciI A'CATG_T
Cuts at: 4768 4768
Size: 5869
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2304 2623 1108
Size: 902 294 319 4354
Fragments arranged by size:
4354 902 319 294
PmeI GTTT'AAAC
Cuts at: 3173 3173
Size: 5869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3938 3970 5451 5483 3938
Size: 32 1481 32 4324
Fragments arranged by size:
4324 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2062 2635 2062
Size: 573 5296
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5869
PsiI TTA'TAA
Cuts at: 3439 3439
Size: 5869
PstI C_TGCA'G
Cuts at: 830 3021 3221 830
Size: 2191 200 3478
Fragments arranged by size:
3478 2191 200
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5869
PvuII CAG'CTG
Cuts at: 2934 2934
Size: 5869
SalI G'TCGA_C
Cuts at: 1936 3211 1936
Size: 1275 4594
SbfI CC_TGCA'GG
Cuts at: 3221 3221
Size: 5869
ScaI AGT'ACT
Cuts at: 2162 2231 3116 4117 5829 2162
Size: 69 885 1001 1712 2202
Fragments arranged by size:
2202 1712 1001 885 69
SexAI A'CCwGG_T
Cuts at: 3144 3144
Size: 5869
SfcI C'TryA_G
Cuts at: 826 1045 3017 3217 4352 5033 5224 826
Size: 219 1972 200 1135 681 191 1471
Fragments arranged by size:
1972 1471 1135 681 219 200 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5869
SmaI CCC'GGG
Cuts at: 1872 3196 1872
Size: 1324 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2086 3973 4874 5136 820
Size: 197 926 143 1887 901 262 1553
Fragments arranged by size:
1887 1553 926 901 262 197 143
SnaBI TAC'GTA
Cuts at: 493 3660 493
Size: 3167 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5869
SphI G_CATG'C
Cuts at: 1132 3227 1132
Size: 2095 3774
SspI AAT'ATT
Cuts at: 5 52 3793 5
Size: 47 3741 2081
Fragments arranged by size:
3741 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5869
StyI C'CwwG_G
Cuts at: 513 1065 3308 513
Size: 552 2243 3074
Fragments arranged by size:
3074 2243 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2296 2722 3016 3083 3896 3913 4066
Size: 1255 716 426 294 67 813 17 153
Cuts at: 4066 4251 4696 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 813 716 445 426 294 185
153 67 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2055 2160 2229
Size: 274 80 33 51 1443 78 105 69
Cuts at: 2229 3114 4115 5827 96
Size: 885 1001 1712 138
Fragments arranged by size:
1712 1443 1001 885 274 138 105 80
78 69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3079 3111 5829 5861 3079
Size: 32 2718 32 3087
Fragments arranged by size:
3087 2718 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2453 2963 1316
Size: 1137 510 4222
Fragments arranged by size:
4222 1137 510
XbaI T'CTAG_A
Cuts at: 3205 3205
Size: 5869
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5869
XmaI C'CCGG_G
Cuts at: 1870 3194 1870
Size: 1324 4545
XmnI GAAnn'nnTTC
Cuts at: 2123 3998 2123
Size: 1875 3994
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3677 276
Size: 53 83 186 936 2143 2468
Fragments arranged by size:
2468 2143 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstXI BstZ17I EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI