(Circular) (Six-base) MAPSORT of: pFN21AE0243.seq Check: 1040 from: 1 to: 5869 pFN21AE0243 5869 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3679 278 Size: 53 83 186 936 2143 2468 Fragments arranged by size: 2468 2143 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2319 2649 3212 1937 Size: 382 330 563 4594 Fragments arranged by size: 4594 563 382 330 Acc65I G'GTAC_C Cuts at: 3190 3190 Size: 5869 AclI AA'CG_TT Cuts at: 1563 3996 4369 1563 Size: 2433 373 3063 Fragments arranged by size: 3063 2433 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2138 2150 3932 5316 5584 1632 Size: 506 12 1782 1384 268 1917 Fragments arranged by size: 1917 1782 1384 506 268 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5672 AflIII A'CryG_T Cuts at: 1203 4768 5745 1203 Size: 3565 977 1327 Fragments arranged by size: 3565 1327 977 AgeI A'CCGG_T Cuts at: 4700 4700 Size: 5869 AhdI GACnn_n'nnGTC Cuts at: 4598 4598 Size: 5869 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5869 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2864 1184 Size: 1680 4189 AlwNI CAG_nnn'CTG Cuts at: 2206 2623 3612 5184 2206 Size: 417 989 1572 2891 Fragments arranged by size: 2891 1572 989 417 ApaLI G'TGCA_C Cuts at: 3926 5082 3926 Size: 1156 4713 ApoI r'AATT_y Cuts at: 1493 3178 3404 3556 5665 1493 Size: 1685 226 152 2109 1697 Fragments arranged by size: 2109 1697 1685 226 152 AseI AT'TA_AT Cuts at: 160 1032 4423 160 Size: 872 3391 1606 Fragments arranged by size: 3391 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 AvaI C'yCGr_G Cuts at: 1870 1943 3194 1870 Size: 73 1251 4545 Fragments arranged by size: 4545 1251 73 BamHI G'GATC_C Cuts at: 3087 3199 3566 3087 Size: 112 367 5390 Fragments arranged by size: 5390 367 112 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2308 2417 2734 2840 3190 Size: 325 217 627 521 109 317 106 350 Cuts at: 3190 4645 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 521 350 325 317 217 109 106 BanII G_rGCy'C Cuts at: 1391 3188 1391 Size: 1797 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2959 3094 3118 928 Size: 971 1060 135 24 3679 Fragments arranged by size: 3679 1060 971 135 24 BbvCI CC'TCA_GC Cuts at: 2654 2811 2654 Size: 157 5712 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4060 4094 1903 Size: 34 2123 34 3678 Fragments arranged by size: 3678 2123 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2438 3761 4971 2438 Size: 1323 1210 3336 Fragments arranged by size: 3336 1323 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5869 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3164 4480 136 Size: 107 122 71 631 605 1492 1316 1525 Fragments arranged by size: 1525 1492 1316 631 605 122 107 71 BglII A'GATC_T Cuts at: 2487 5864 2487 Size: 3377 2492 BlpI GC'TnA_GC Cuts at: 2938 3286 2938 Size: 348 5521 Bme1580I G_kGCm'C Cuts at: 1790 2313 3930 5086 1790 Size: 523 1617 1156 2573 Fragments arranged by size: 2573 1617 1156 523 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5869 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2164 2740 2997 Size: 416 53 83 399 24 747 576 257 Cuts at: 2997 3600 4558 442 Size: 603 958 1753 Fragments arranged by size: 1753 958 747 603 576 416 399 257 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5869 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2419 2419 Size: 5869 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2228 2311 3096 4529 1239 Size: 989 83 785 1433 2579 Fragments arranged by size: 2579 1433 989 785 83 Bpu10I CC'TnA_GC Cuts at: 2498 2654 2811 2498 Size: 156 157 5556 Fragments arranged by size: 5556 157 156 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2107 3994 4859 5157 2107 Size: 1887 865 298 2819 Fragments arranged by size: 2819 1887 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4532 882 Size: 617 3033 2219 Fragments arranged by size: 3033 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3660 493 Size: 711 2456 2702 Fragments arranged by size: 2702 2456 711 BsaBI GATnn'nnATC Cuts at: 5863 5863 Size: 5869 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3676 4058 4693 275 Size: 53 83 186 936 2143 382 635 1451 Fragments arranged by size: 2143 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4302 4700 4974 5121 5532 4302 Size: 398 274 147 411 4639 Fragments arranged by size: 4639 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2276 2306 1178 Size: 30 165 30 873 30 4741 Fragments arranged by size: 4741 873 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2284 2569 1186 Size: 1098 285 4486 Fragments arranged by size: 4486 1098 285 BseYI C'CCAG_C Cuts at: 2023 2149 2567 5072 5568 2023 Size: 126 418 2505 496 2324 Fragments arranged by size: 2505 2324 496 418 126 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2323 1163 Size: 573 587 4709 Fragments arranged by size: 4709 587 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3319 4080 5108 1540 Size: 275 186 1318 761 1028 2301 Fragments arranged by size: 2301 1318 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3930 4015 5086 3930 Size: 85 1071 4713 Fragments arranged by size: 4713 1071 85 BsmI GAATG_Cn' Cuts at: 3380 3473 3380 Size: 93 5776 BsmBI CGTCTCn'nnnn_ Cuts at: 2498 2498 Size: 5869 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2313 3188 3930 4015 5086 1391 Size: 399 523 875 742 85 1071 2174 Fragments arranged by size: 2174 1071 875 742 523 399 85 BspEI T'CCGG_A Cuts at: 5532 5532 Size: 5869 BspHI T'CATG_A Cuts at: 3758 5488 3758 Size: 1730 4139 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3224 844 Size: 2380 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3756 1394 Size: 429 1933 3507 Fragments arranged by size: 3507 1933 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2871 4364 4538 65 Size: 1183 1623 1493 174 1396 Fragments arranged by size: 1623 1493 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2288 2420 2837 2855 4513 4700 1700 Size: 110 478 132 417 18 1658 187 2869 Fragments arranged by size: 2869 1658 478 417 187 132 110 18 BsrGI T'GTAC_A Cuts at: 96 2055 96 Size: 1959 3910 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5869 BssSI C'ACGA_G Cuts at: 3929 4941 3929 Size: 1012 4857 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5869 BstBI TT'CG_AA Cuts at: 4673 4673 Size: 5869 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5869 BstYI r'GATC_y Cuts at: 1972 2487 3087 3199 3566 3951 3968 5420 5506 Size: 515 600 112 367 385 17 1452 86 Cuts at: 5506 5518 5864 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 600 515 385 367 346 112 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2364 2364 Size: 5869 BtgI C'CryG_G Cuts at: 513 1065 2769 3162 513 Size: 552 1704 393 3220 Fragments arranged by size: 3220 1704 552 393 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2785 487 Size: 683 56 1559 3571 Fragments arranged by size: 3571 1559 683 56 BtsI GCAGTG_nn' Cuts at: 3385 4179 4199 3385 Size: 794 20 5055 Fragments arranged by size: 5055 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5869 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5834 DraI TTT'AAA Cuts at: 3173 3173 Size: 5869 DraIII CAC_nnn'GTG Cuts at: 3005 3005 Size: 5869 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2927 4876 809 Size: 507 1611 1949 1802 Fragments arranged by size: 1949 1802 1611 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3316 4205 5834 Size: 54 1425 50 275 18 1486 889 1629 Cuts at: 5834 8 Size: 43 Fragments arranged by size: 1629 1486 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3316 1537 Size: 275 1504 4090 Fragments arranged by size: 4090 1504 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2645 2710 3799 1353 Size: 1292 65 1089 3423 Fragments arranged by size: 3423 1292 1089 65 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3098 4456 4830 4976 387 Size: 1064 138 1509 1358 374 146 1280 Fragments arranged by size: 1509 1358 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2150 2228 2311 3096 3932 4529 Size: 393 506 12 78 83 785 836 597 Cuts at: 4529 5316 5584 1239 Size: 787 268 1524 Fragments arranged by size: 1524 836 787 785 597 506 393 268 83 78 12 EcoNI CCTnn'n_nnAGG Cuts at: 2117 2117 Size: 5869 EcoO109I rG'GnC_Cy Cuts at: 2062 2620 2635 2062 Size: 558 15 5296 Fragments arranged by size: 5296 558 15 EcoRI G'AATT_C Cuts at: 3178 3556 3178 Size: 378 5491 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2101 2101 Size: 5869 FspI TGC'GCA Cuts at: 4375 4375 Size: 5869 HaeII r_GCGC'y Cuts at: 1678 2039 2661 5016 1678 Size: 361 622 2355 2531 Fragments arranged by size: 2531 2355 622 361 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2276 2308 2627 2659 2784 Size: 32 135 32 704 32 319 32 125 Cuts at: 2784 2816 4510 4542 4584 4616 1373 Size: 32 1694 32 42 32 2626 Fragments arranged by size: 2626 1694 704 319 135 125 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2833 3213 3459 669 Size: 988 281 895 380 246 3079 Fragments arranged by size: 3079 988 895 380 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5869 HpaI GTT'AAC Cuts at: 3459 3459 Size: 5869 KpnI G_GTAC'C Cuts at: 3194 3194 Size: 5869 MfeI C'AATT_G Cuts at: 3468 3468 Size: 5869 MluI A'CGCG_T Cuts at: 5745 5745 Size: 5869 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2322 2443 4983 5167 1305 Size: 1017 121 2540 184 2007 Fragments arranged by size: 2540 2007 1017 184 121 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4047 Fragments arranged by size: 4047 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2783 3827 4186 4345 518 Size: 648 1617 1044 359 159 2042 Fragments arranged by size: 2042 1617 1044 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2934 3285 3962 5110 5355 1606 Size: 1328 351 677 1148 245 2120 Fragments arranged by size: 2120 1328 1148 677 351 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5317 NdeI CA'TA_TG Cuts at: 387 387 Size: 5869 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5869 NotI GC'GGCC_GC Cuts at: 3316 3316 Size: 5869 NspI r_CATG'y Cuts at: 1132 1229 3227 4772 1132 Size: 97 1998 1545 2229 Fragments arranged by size: 2229 1998 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5869 PciI A'CATG_T Cuts at: 4768 4768 Size: 5869 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2304 2623 1108 Size: 902 294 319 4354 Fragments arranged by size: 4354 902 319 294 PmeI GTTT'AAAC Cuts at: 3173 3173 Size: 5869 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3938 3970 5451 5483 3938 Size: 32 1481 32 4324 Fragments arranged by size: 4324 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2062 2635 2062 Size: 573 5296 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5869 PsiI TTA'TAA Cuts at: 3439 3439 Size: 5869 PstI C_TGCA'G Cuts at: 830 3021 3221 830 Size: 2191 200 3478 Fragments arranged by size: 3478 2191 200 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5869 PvuII CAG'CTG Cuts at: 2934 2934 Size: 5869 SalI G'TCGA_C Cuts at: 1936 3211 1936 Size: 1275 4594 SbfI CC_TGCA'GG Cuts at: 3221 3221 Size: 5869 ScaI AGT'ACT Cuts at: 2162 2231 3116 4117 5829 2162 Size: 69 885 1001 1712 2202 Fragments arranged by size: 2202 1712 1001 885 69 SexAI A'CCwGG_T Cuts at: 3144 3144 Size: 5869 SfcI C'TryA_G Cuts at: 826 1045 3017 3217 4352 5033 5224 826 Size: 219 1972 200 1135 681 191 1471 Fragments arranged by size: 1972 1471 1135 681 219 200 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5869 SmaI CCC'GGG Cuts at: 1872 3196 1872 Size: 1324 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2086 3973 4874 5136 820 Size: 197 926 143 1887 901 262 1553 Fragments arranged by size: 1887 1553 926 901 262 197 143 SnaBI TAC'GTA Cuts at: 493 3660 493 Size: 3167 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5869 SphI G_CATG'C Cuts at: 1132 3227 1132 Size: 2095 3774 SspI AAT'ATT Cuts at: 5 52 3793 5 Size: 47 3741 2081 Fragments arranged by size: 3741 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5869 StyI C'CwwG_G Cuts at: 513 1065 3308 513 Size: 552 2243 3074 Fragments arranged by size: 3074 2243 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2296 2722 3016 3083 3896 3913 4066 Size: 1255 716 426 294 67 813 17 153 Cuts at: 4066 4251 4696 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 813 716 445 426 294 185 153 67 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2055 2160 2229 Size: 274 80 33 51 1443 78 105 69 Cuts at: 2229 3114 4115 5827 96 Size: 885 1001 1712 138 Fragments arranged by size: 1712 1443 1001 885 274 138 105 80 78 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3079 3111 5829 5861 3079 Size: 32 2718 32 3087 Fragments arranged by size: 3087 2718 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2453 2963 1316 Size: 1137 510 4222 Fragments arranged by size: 4222 1137 510 XbaI T'CTAG_A Cuts at: 3205 3205 Size: 5869 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5869 XmaI C'CCGG_G Cuts at: 1870 3194 1870 Size: 1324 4545 XmnI GAAnn'nnTTC Cuts at: 2123 3998 2123 Size: 1875 3994 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3677 276 Size: 53 83 186 936 2143 2468 Fragments arranged by size: 2468 2143 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI