Restriction map for
pFN21AE0241
(Circular) (Six-base) MAPSORT of: pFN21AE0241.seq Check: 3386 from: 1 to: 5986
pFN21AE0241 5986 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3796 278
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3329 1937
Size: 1392 4594
Acc65I G'GTAC_C
Cuts at: 2205 3307 2205
Size: 1102 4884
AclI AA'CG_TT
Cuts at: 1563 4113 4486 1563
Size: 2550 373 3063
Fragments arranged by size:
3063 2550 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2028 2572 2739 4049 5433 5701 1632
Size: 396 544 167 1310 1384 268 1917
Fragments arranged by size:
1917 1384 1310 544 396 268 167
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5789
AflIII A'CryG_T
Cuts at: 1203 2228 4885 5862 1203
Size: 1025 2657 977 1327
Fragments arranged by size:
2657 1327 1025 977
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 4715 4715
Size: 5986
AleI CACnn'nnGTG
Cuts at: 1166 2231 1166
Size: 1065 4921
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5986
AlwNI CAG_nnn'CTG
Cuts at: 3729 5301 3729
Size: 1572 4414
ApaLI G'TGCA_C
Cuts at: 4043 5199 4043
Size: 1156 4830
ApoI r'AATT_y
Cuts at: 1493 3118 3295 3521 3673 5782 1493
Size: 1625 177 226 152 2109 1697
Fragments arranged by size:
2109 1697 1625 226 177 152
AseI AT'TA_AT
Cuts at: 160 1032 4540 160
Size: 872 3508 1606
Fragments arranged by size:
3508 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 3311 1870
Size: 73 1368 4545
Fragments arranged by size:
4545 1368 73
BamHI G'GATC_C
Cuts at: 3316 3683 3316
Size: 367 5619
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2205 3307 4762 618
Size: 325 217 627 418 1102 1455 1842
Fragments arranged by size:
1842 1455 1102 627 418 325 217
BanII G_rGCy'C
Cuts at: 1391 3258 3305 1391
Size: 1867 47 4072
Fragments arranged by size:
4072 1867 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2058 2578 2835 3171 928
Size: 971 159 520 257 336 3743
Fragments arranged by size:
3743 971 520 336 257 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2112 2146 4177 4211 1903
Size: 34 175 34 2031 34 3678
Fragments arranged by size:
3678 2031 175 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3878 5088 3878
Size: 1210 4776
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5986
BfrBI ATG'CAT
Cuts at: 2348 2348
Size: 5986
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2075 2552 3268
Size: 107 122 71 631 605 403 477 716
Cuts at: 3268 4597 136
Size: 1329 1525
Fragments arranged by size:
1525 1329 716 631 605 477 403 122
107 71
BglII A'GATC_T
Cuts at: 5981 5981
Size: 5986
BlpI GC'TnA_GC
Cuts at: 3403 3403
Size: 5986
Bme1580I G_kGCm'C
Cuts at: 1790 4047 5203 1790
Size: 2257 1156 2573
Fragments arranged by size:
2573 2257 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5986
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3717 4675 442
Size: 416 53 83 399 24 2300 958 1753
Fragments arranged by size:
2300 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5986
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2711 2711
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4646 1239
Size: 3407 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4111 4976 5274 4111
Size: 865 298 4823
Fragments arranged by size:
4823 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2411 4649 882
Size: 617 912 2238 2219
Fragments arranged by size:
2238 2219 912 617
BsaAI yAC'GTr
Cuts at: 493 1204 2229 3777 493
Size: 711 1025 1548 2702
Fragments arranged by size:
2702 1548 1025 711
BsaBI GATnn'nnATC
Cuts at: 5980 5980
Size: 5986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3793 4175 4810 275
Size: 53 83 186 936 2260 382 635 1451
Fragments arranged by size:
2260 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4419 4817 5091 5238 5649 4419
Size: 398 274 147 411 4756
Fragments arranged by size:
4756 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5761
Fragments arranged by size:
5761 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2687 1186
Size: 1501 4485
BseYI C'CCAG_C
Cuts at: 5189 5685 5189
Size: 496 5490
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2152 1163
Size: 573 416 4997
Fragments arranged by size:
4997 573 416
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2782 3436 4197 5225 1540
Size: 275 186 781 654 761 1028 2301
Fragments arranged by size:
2301 1028 781 761 654 275 186
BsiHKAI G_wGCw'C
Cuts at: 2692 2994 4047 4132 5203 2692
Size: 302 1053 85 1071 3475
Fragments arranged by size:
3475 1071 1053 302 85
BsmI GAATG_Cn'
Cuts at: 2468 3497 3590 2468
Size: 1029 93 4864
Fragments arranged by size:
4864 1029 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2044 2610 2044
Size: 566 5420
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2692 2994 3258 3305 4047 4132 5203
Size: 399 902 302 264 47 742 85 1071
Cuts at: 5203 1391
Size: 2174
Fragments arranged by size:
2174 1071 902 742 399 302 264 85
47
BspEI T'CCGG_A
Cuts at: 5649 5649
Size: 5986
BspHI T'CATG_A
Cuts at: 3875 5605 3875
Size: 1730 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3341 844
Size: 2497 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3873 1394
Size: 429 2050 3507
Fragments arranged by size:
3507 2050 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4481 4655 65
Size: 1183 3233 174 1396
Fragments arranged by size:
3233 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4630 4817 1700
Size: 110 2820 187 2869
Fragments arranged by size:
2869 2820 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5986
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5986
BssSI C'ACGA_G
Cuts at: 4046 5058 4046
Size: 1012 4974
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5986
BstBI TT'CG_AA
Cuts at: 4790 4790
Size: 5986
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5986
BstXI CCAn_nnnn'nTGG
Cuts at: 2233 2233
Size: 5986
BstYI r'GATC_y
Cuts at: 1972 3316 3683 4068 4085 5537 5623 5635 5981
Size: 1344 367 385 17 1452 86 12 346
Cuts at: 5981 1972
Size: 1977
Fragments arranged by size:
1977 1452 1344 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2073 2332 513
Size: 552 939 69 259 4167
Fragments arranged by size:
4167 939 552 259 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5247
Fragments arranged by size:
5247 683 56
BtsI GCAGTG_nn'
Cuts at: 2017 2916 3502 4296 4316 2017
Size: 899 586 794 20 3687
Fragments arranged by size:
3687 899 794 586 20
ClaI AT'CG_AT
Cuts at: 1554 2517 2778 1554
Size: 963 261 4762
Fragments arranged by size:
4762 963 261
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5951
DraI TTT'AAA
Cuts at: 3290 3290
Size: 5986
DraIII CAC_nnn'GTG
Cuts at: 2229 2671 2229
Size: 442 5544
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4993 809
Size: 507 3677 1802
Fragments arranged by size:
3677 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2158 2544
Size: 54 1425 50 275 18 237 91 386
Cuts at: 2544 3433 4322 5951 8
Size: 889 889 1629 43
Fragments arranged by size:
1629 1425 889 889 386 275 237 91
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3433 1537
Size: 275 1621 4090
Fragments arranged by size:
4090 1621 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2047 2099 2411 2561 2994 3916 1353
Size: 694 52 312 150 433 922 3423
Fragments arranged by size:
3423 922 694 433 312 150 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4573 4947 5093 387
Size: 1064 138 2984 374 146 1280
Fragments arranged by size:
2984 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2028 2572 2739 4049 4646 5433 5701
Size: 393 396 544 167 1310 597 787 268
Cuts at: 5701 1239
Size: 1524
Fragments arranged by size:
1524 1310 787 597 544 396 393 268
167
EcoO109I rG'GnC_Cy
Cuts at: 2338 3261 2338
Size: 923 5063
EcoRI G'AATT_C
Cuts at: 3295 3673 3295
Size: 378 5608
FspI TGC'GCA
Cuts at: 4492 4492
Size: 5986
HaeII r_GCGC'y
Cuts at: 1678 5133 1678
Size: 3455 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4627 4659 4701 4733 1373
Size: 32 135 32 3055 32 42 32 2626
Fragments arranged by size:
3055 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2383 3330 3576 669
Size: 988 281 445 947 246 3079
Fragments arranged by size:
3079 988 947 445 281 246
HindIII A'AGCT_T
Cuts at: 748 2364 748
Size: 1616 4370
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KpnI G_GTAC'C
Cuts at: 2209 3311 2209
Size: 1102 4884
MfeI C'AATT_G
Cuts at: 2469 3585 2469
Size: 1116 4870
MluI A'CGCG_T
Cuts at: 5862 5862
Size: 5986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3258 5100 5284 1305
Size: 1953 1842 184 2007
Fragments arranged by size:
2007 1953 1842 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2069 2160 2546 10
Size: 54 1425 343 237 91 386 3450
Fragments arranged by size:
3450 1425 386 343 237 91 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2166 2183 2231 2486 2921 2979 3944
Size: 648 1000 17 48 255 435 58 965
Cuts at: 3944 4303 4462 518
Size: 359 159 2042
Fragments arranged by size:
2042 1000 965 648 435 359 255 159
58 48 17
MspA1I CmG'CkG
Cuts at: 1606 3402 4079 5227 5472 1606
Size: 1796 677 1148 245 2120
Fragments arranged by size:
2120 1796 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2073 2332 513
Size: 552 939 69 259 4167
Fragments arranged by size:
4167 939 552 259 69
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5986
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5986
NotI GC'GGCC_GC
Cuts at: 3433 3433
Size: 5986
NsiI A_TGCA'T
Cuts at: 2350 2350
Size: 5986
NspI r_CATG'y
Cuts at: 1132 1229 3344 4889 1132
Size: 97 2115 1545 2229
Fragments arranged by size:
2229 2115 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5986
PciI A'CATG_T
Cuts at: 4885 4885
Size: 5986
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2671 1108
Size: 1563 4423
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PmlI CAC'GTG
Cuts at: 2229 2229
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 5568 5600 4055
Size: 32 1481 32 4441
Fragments arranged by size:
4441 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2338 2338
Size: 5986
PshAI GACnn'nnGTC
Cuts at: 1613 2582 1613
Size: 969 5017
PsiI TTA'TAA
Cuts at: 3556 3556
Size: 5986
PstI C_TGCA'G
Cuts at: 830 3338 830
Size: 2508 3478
PvuI CG_AT'CG
Cuts at: 2001 2782 2001
Size: 781 5205
SalI G'TCGA_C
Cuts at: 1936 3328 1936
Size: 1392 4594
SbfI CC_TGCA'GG
Cuts at: 3338 3338
Size: 5986
ScaI AGT'ACT
Cuts at: 4234 5946 4234
Size: 1712 4274
SfcI C'TryA_G
Cuts at: 826 1045 2029 2808 3334 4469 5150 5341 826
Size: 219 984 779 526 1135 681 191 1471
Fragments arranged by size:
1471 1135 984 779 681 526 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5986
SmaI CCC'GGG
Cuts at: 1872 3313 1872
Size: 1441 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4090 4991 5253 820
Size: 197 926 2147 901 262 1553
Fragments arranged by size:
2147 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3777 493
Size: 3284 2702
SpeI A'CTAG_T
Cuts at: 152 3197 152
Size: 3045 2941
SphI G_CATG'C
Cuts at: 1132 3344 1132
Size: 2212 3774
SspI AAT'ATT
Cuts at: 5 52 3910 5
Size: 47 3858 2081
Fragments arranged by size:
3858 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5986
StyI C'CwwG_G
Cuts at: 513 1065 2004 2073 2332 3425 513
Size: 552 939 69 259 1093 3074
Fragments arranged by size:
3074 1093 939 552 259 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2177 4013 4030 4183 4368 4813 325
Size: 1255 597 1836 17 153 185 445 1498
Fragments arranged by size:
1836 1498 1255 597 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4232 5944 96
Size: 274 80 33 51 1443 2255 1712 138
Fragments arranged by size:
2255 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5946 5978 5946
Size: 32 5954
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5986
XbaI T'CTAG_A
Cuts at: 3250 3322 3250
Size: 72 5914
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5986
XmaI C'CCGG_G
Cuts at: 1870 3311 1870
Size: 1441 4545
XmnI GAAnn'nnTTC
Cuts at: 2713 4115 2713
Size: 1402 4584
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3794 276
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI