Restriction map for
pFN21AE0239
(Circular) (Six-base) MAPSORT of: pFN21AE0239.seq Check: 7312 from: 1 to: 5530
pFN21AE0239 5530 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3340 278
Size: 53 83 186 936 1804 2468
Fragments arranged by size:
2468 1804 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2873 1937
Size: 936 4594
Acc65I G'GTAC_C
Cuts at: 2851 2851
Size: 5530
AclI AA'CG_TT
Cuts at: 1563 3657 4030 1563
Size: 2094 373 3063
Fragments arranged by size:
3063 2094 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2354 2601 3593 4977 5245 1632
Size: 722 247 992 1384 268 1917
Fragments arranged by size:
1917 1384 992 722 268 247
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5333
AflIII A'CryG_T
Cuts at: 1203 4429 5406 1203
Size: 3226 977 1327
Fragments arranged by size:
3226 1327 977
AgeI A'CCGG_T
Cuts at: 4361 4361
Size: 5530
AhdI GACnn_n'nnGTC
Cuts at: 4259 4259
Size: 5530
AleI CACnn'nnGTG
Cuts at: 1166 2467 1166
Size: 1301 4229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5530
AlwNI CAG_nnn'CTG
Cuts at: 3273 4845 3273
Size: 1572 3958
ApaLI G'TGCA_C
Cuts at: 2126 2336 3587 4743 2126
Size: 210 1251 1156 2913
Fragments arranged by size:
2913 1251 1156 210
ApoI r'AATT_y
Cuts at: 1493 2519 2839 3065 3217 5326 1493
Size: 1026 320 226 152 2109 1697
Fragments arranged by size:
2109 1697 1026 320 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4084 160
Size: 872 3052 1606
Fragments arranged by size:
3052 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5530
AvaI C'yCGr_G
Cuts at: 1870 1943 2855 1870
Size: 73 912 4545
Fragments arranged by size:
4545 912 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2019 2052 2019
Size: 33 5497
BamHI G'GATC_C
Cuts at: 2860 3227 2860
Size: 367 5163
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2663 2851 4306 618
Size: 325 217 627 876 188 1455 1842
Fragments arranged by size:
1842 1455 876 627 325 217 188
BanII G_rGCy'C
Cuts at: 1391 2028 2849 1391
Size: 637 821 4072
Fragments arranged by size:
4072 821 637
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2360 928
Size: 971 461 4098
Fragments arranged by size:
4098 971 461
BbvCI CC'TCA_GC
Cuts at: 2387 2387
Size: 5530
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3721 3755 1903
Size: 34 1784 34 3678
Fragments arranged by size:
3678 1784 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3422 4632 3422
Size: 1210 4320
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5530
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4141 136
Size: 107 122 71 631 605 2469 1525
Fragments arranged by size:
2469 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5525 5525
Size: 5530
BlpI GC'TnA_GC
Cuts at: 2249 2947 2249
Size: 698 4832
Bme1580I G_kGCm'C
Cuts at: 1790 2130 2340 2668 3591 4747 1790
Size: 340 210 328 923 1156 2573
Fragments arranged by size:
2573 1156 923 340 328 210
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5530
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3261 4219 442
Size: 416 53 83 399 24 1844 958 1753
Fragments arranged by size:
1844 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2172 4190 1239
Size: 933 2018 2579
Fragments arranged by size:
2579 2018 933
Bpu10I CC'TnA_GC
Cuts at: 2387 2387
Size: 5530
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3655 4520 4818 3655
Size: 865 298 4367
Fragments arranged by size:
4367 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2106 4193 882
Size: 617 607 2087 2219
Fragments arranged by size:
2219 2087 617 607
BsaAI yAC'GTr
Cuts at: 493 1204 3321 493
Size: 711 2117 2702
Fragments arranged by size:
2702 2117 711
BsaBI GATnn'nnATC
Cuts at: 5524 5524
Size: 5530
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3337 3719 4354 275
Size: 53 83 186 936 1804 382 635 1451
Fragments arranged by size:
1804 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3963 4361 4635 4782 5193 3963
Size: 398 274 147 411 4300
Fragments arranged by size:
4300 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5305
Fragments arranged by size:
5305 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2815 2818 1186
Size: 1629 3 3898
Fragments arranged by size:
3898 1629 3
BseYI C'CCAG_C
Cuts at: 2173 4733 5229 2173
Size: 2560 496 2474
Fragments arranged by size:
2560 2474 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2260 1163
Size: 573 524 4433
Fragments arranged by size:
4433 573 524
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2980 3741 4769 1540
Size: 275 186 979 761 1028 2301
Fragments arranged by size:
2301 1028 979 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2028 2130 2340 2506 3591 3676 4747 2028
Size: 102 210 166 1085 85 1071 2811
Fragments arranged by size:
2811 1085 1071 210 166 102 85
BsmI GAATG_Cn'
Cuts at: 2253 3041 3134 2253
Size: 788 93 4649
Fragments arranged by size:
4649 788 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2130 2340 2506 2668 2849 3591
Size: 399 238 102 210 166 162 181 742
Cuts at: 3591 3676 4747 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 238 210 181 166
162 102 85
BspEI T'CCGG_A
Cuts at: 5193 5193
Size: 5530
BspHI T'CATG_A
Cuts at: 3419 5149 3419
Size: 1730 3800
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2282 2885 844
Size: 1438 603 3489
Fragments arranged by size:
3489 1438 603
BsrBI CCG'CTC
Cuts at: 1394 1823 3417 1394
Size: 429 1594 3507
Fragments arranged by size:
3507 1594 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4025 4199 65
Size: 1183 2777 174 1396
Fragments arranged by size:
2777 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4174 4361 1700
Size: 110 2364 187 2869
Fragments arranged by size:
2869 2364 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5530
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5530
BssSI C'ACGA_G
Cuts at: 3590 4602 3590
Size: 1012 4518
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5530
BstBI TT'CG_AA
Cuts at: 4334 4334
Size: 5530
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5530
BstYI r'GATC_y
Cuts at: 1972 2860 3227 3612 3629 5081 5167 5179 5525
Size: 888 367 385 17 1452 86 12 346
Cuts at: 5525 1972
Size: 1977
Fragments arranged by size:
1977 1452 888 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2077 2077
Size: 5530
BtgI C'CryG_G
Cuts at: 513 1065 2344 513
Size: 552 1279 3699
Fragments arranged by size:
3699 1279 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4791
Fragments arranged by size:
4791 683 56
BtsI GCAGTG_nn'
Cuts at: 2028 2277 2338 3046 3840 3860 2028
Size: 249 61 708 794 20 3698
Fragments arranged by size:
3698 794 708 249 61 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5530
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5495
DraI TTT'AAA
Cuts at: 2834 2834
Size: 5530
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4537 809
Size: 507 3221 1802
Fragments arranged by size:
3221 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2977 3866 5495
Size: 54 1425 50 275 18 1147 889 1629
Cuts at: 5495 8
Size: 43
Fragments arranged by size:
1629 1425 1147 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2977 1537
Size: 275 1165 4090
Fragments arranged by size:
4090 1165 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 2421 2809 3460 1353
Size: 728 340 388 651 3423
Fragments arranged by size:
3423 728 651 388 340
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4117 4491 4637 387
Size: 1064 138 2528 374 146 1280
Fragments arranged by size:
2528 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2026 2026
Size: 5530
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2172 2354 2601 3593 4190 4977 5245
Size: 393 540 182 247 992 597 787 268
Cuts at: 5245 1239
Size: 1524
Fragments arranged by size:
1524 992 787 597 540 393 268 247
182
EcoNI CCTnn'n_nnAGG
Cuts at: 2076 2076
Size: 5530
EcoRI G'AATT_C
Cuts at: 2519 2839 3217 2519
Size: 320 378 4832
Fragments arranged by size:
4832 378 320
EcoRV GAT'ATC
Cuts at: 2296 2296
Size: 5530
FspI TGC'GCA
Cuts at: 4036 4036
Size: 5530
HaeII r_GCGC'y
Cuts at: 1678 4677 1678
Size: 2999 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4171 4203 4245 4277 1373
Size: 32 135 32 2599 32 42 32 2626
Fragments arranged by size:
2626 2599 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2874 3120 669
Size: 988 281 936 246 3079
Fragments arranged by size:
3079 988 936 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5530
HpaI GTT'AAC
Cuts at: 3120 3120
Size: 5530
KpnI G_GTAC'C
Cuts at: 2855 2855
Size: 5530
MfeI C'AATT_G
Cuts at: 3129 3129
Size: 5530
MluI A'CGCG_T
Cuts at: 5406 5406
Size: 5530
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4644 4828 1305
Size: 3339 184 2007
Fragments arranged by size:
3339 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3708
Fragments arranged by size:
3708 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2421 2467 2535 3488 3847 4006 518
Size: 648 1255 46 68 953 359 159 2042
Fragments arranged by size:
2042 1255 953 648 359 159 68 46
MspA1I CmG'CkG
Cuts at: 1606 2946 3623 4771 5016 1606
Size: 1340 677 1148 245 2120
Fragments arranged by size:
2120 1340 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4978
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5530
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5530
NotI GC'GGCC_GC
Cuts at: 2977 2977
Size: 5530
NspI r_CATG'y
Cuts at: 1132 1229 2888 4433 1132
Size: 97 1659 1545 2229
Fragments arranged by size:
2229 1659 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5530
PciI A'CATG_T
Cuts at: 4429 4429
Size: 5530
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5530
PmeI GTTT'AAAC
Cuts at: 2834 2834
Size: 5530
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3599 3631 5112 5144 3599
Size: 32 1481 32 3985
Fragments arranged by size:
3985 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5530
PsiI TTA'TAA
Cuts at: 2421 2597 3100 2421
Size: 176 503 4851
Fragments arranged by size:
4851 503 176
PstI C_TGCA'G
Cuts at: 830 2035 2882 830
Size: 1205 847 3478
Fragments arranged by size:
3478 1205 847
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5530
SacI G_AGCT'C
Cuts at: 2028 2028
Size: 5530
SalI G'TCGA_C
Cuts at: 1936 2872 1936
Size: 936 4594
SapI GCTCTTCn'nnn_
Cuts at: 2809 2809
Size: 5530
SbfI CC_TGCA'GG
Cuts at: 2882 2882
Size: 5530
ScaI AGT'ACT
Cuts at: 3778 5490 3778
Size: 1712 3818
SfcI C'TryA_G
Cuts at: 826 1045 2031 2101 2560 2730 2878 4013 4694
Size: 219 986 70 459 170 148 1135 681
Cuts at: 4694 4885 826
Size: 191 1471
Fragments arranged by size:
1471 1135 986 681 459 219 191 170
148 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5530
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5530
SmaI CCC'GGG
Cuts at: 1872 2857 1872
Size: 985 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3634 4535 4797 820
Size: 197 926 1691 901 262 1553
Fragments arranged by size:
1691 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3321 493
Size: 2828 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5530
SphI G_CATG'C
Cuts at: 1132 2888 1132
Size: 1756 3774
SspI AAT'ATT
Cuts at: 5 52 3454 5
Size: 47 3402 2081
Fragments arranged by size:
3402 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5530
StyI C'CwwG_G
Cuts at: 513 1065 2969 513
Size: 552 1904 3074
Fragments arranged by size:
3074 1904 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2175 3557 3574 3727 3912 4357 325
Size: 1255 595 1382 17 153 185 445 1498
Fragments arranged by size:
1498 1382 1255 595 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3776 5488 96
Size: 274 80 33 51 1443 1799 1712 138
Fragments arranged by size:
1799 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5490 5522 5490
Size: 32 5498
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5530
XbaI T'CTAG_A
Cuts at: 2866 2866
Size: 5530
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5530
XmaI C'CCGG_G
Cuts at: 1870 2855 1870
Size: 985 4545
XmnI GAAnn'nnTTC
Cuts at: 3659 3659
Size: 5530
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3338 276
Size: 53 83 186 936 1804 2468
Fragments arranged by size:
2468 1804 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoO109I FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI