Restriction map for
pFN21AE0237
(Circular) (Six-base) MAPSORT of: pFN21AE0237.seq Check: 992 from: 1 to: 5179
pFN21AE0237 5179 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2989 278
Size: 53 83 186 936 1453 2468
Fragments arranged by size:
2468 1453 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2522 1937
Size: 585 4594
Acc65I G'GTAC_C
Cuts at: 2500 2500
Size: 5179
AclI AA'CG_TT
Cuts at: 1563 3306 3679 1563
Size: 1743 373 3063
Fragments arranged by size:
3063 1743 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2394 3242 4626 4894 1632
Size: 762 848 1384 268 1917
Fragments arranged by size:
1917 1384 848 762 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4982
AflIII A'CryG_T
Cuts at: 1203 4078 5055 1203
Size: 2875 977 1327
Fragments arranged by size:
2875 1327 977
AgeI A'CCGG_T
Cuts at: 4010 4010
Size: 5179
AhdI GACnn_n'nnGTC
Cuts at: 3908 3908
Size: 5179
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5179
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5179
AlwNI CAG_nnn'CTG
Cuts at: 2922 4494 2922
Size: 1572 3607
ApaI G_GGCC'C
Cuts at: 2079 2261 2079
Size: 182 4997
ApaLI G'TGCA_C
Cuts at: 3236 4392 3236
Size: 1156 4023
ApoI r'AATT_y
Cuts at: 1493 2488 2714 2866 4975 1493
Size: 995 226 152 2109 1697
Fragments arranged by size:
2109 1697 995 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3733 160
Size: 872 2701 1606
Fragments arranged by size:
2701 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5179
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2140 2353 2402 2504 1870
Size: 73 114 83 213 49 102 4545
Fragments arranged by size:
4545 213 114 102 83 73 49
BamHI G'GATC_C
Cuts at: 2509 2876 2509
Size: 367 4812
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2116 2161 2188 2500 3955
Size: 325 217 627 329 45 27 312 1455
Cuts at: 3955 618
Size: 1842
Fragments arranged by size:
1842 1455 627 329 325 312 217 45
27
BanII G_rGCy'C
Cuts at: 1391 2079 2261 2271 2498 1391
Size: 688 182 10 227 4072
Fragments arranged by size:
4072 688 227 182 10
BbeI G_GCGC'C
Cuts at: 2165 2192 2165
Size: 27 5152
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4208
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3370 3404 1903
Size: 34 1433 34 3678
Fragments arranged by size:
3678 1433 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3071 4281 3071
Size: 1210 3969
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5179
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2246 2407 3790
Size: 107 122 71 631 605 574 161 1383
Cuts at: 3790 136
Size: 1525
Fragments arranged by size:
1525 1383 631 605 574 161 122 107
71
BglII A'GATC_T
Cuts at: 5174 5174
Size: 5179
BlpI GC'TnA_GC
Cuts at: 2596 2596
Size: 5179
Bme1580I G_kGCm'C
Cuts at: 1790 2079 2261 3240 4396 1790
Size: 289 182 979 1156 2573
Fragments arranged by size:
2573 1156 979 289 182
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5179
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2910 3868 442
Size: 416 53 83 399 24 1493 958 1753
Fragments arranged by size:
1753 1493 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 3839 1239
Size: 875 1725 2579
Fragments arranged by size:
2579 1725 875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3304 4169 4467 3304
Size: 865 298 4016
Fragments arranged by size:
4016 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3842 882
Size: 617 2343 2219
Fragments arranged by size:
2343 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2970 493
Size: 711 1766 2702
Fragments arranged by size:
2702 1766 711
BsaBI GATnn'nnATC
Cuts at: 5173 5173
Size: 5179
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2162 2189 2986 3368
Size: 53 83 186 936 629 27 797 382
Cuts at: 3368 4003 275
Size: 635 1451
Fragments arranged by size:
1451 936 797 635 629 382 186 83
53 27
BsaWI w'CCGG_w
Cuts at: 3612 4010 4284 4431 4842 3612
Size: 398 274 147 411 3949
Fragments arranged by size:
3949 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4954
Fragments arranged by size:
4954 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2171 1186
Size: 985 4194
BseYI C'CCAG_C
Cuts at: 2177 2284 4382 4878 2177
Size: 107 2098 496 2478
Fragments arranged by size:
2478 2098 496 107
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4606
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2133 2170 2310 2392 2629 3390
Size: 275 186 132 37 140 82 237 761
Cuts at: 3390 4418 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 275 237 186 140 132
82 37
BsiHKAI G_wGCw'C
Cuts at: 2342 3240 3325 4396 2342
Size: 898 85 1071 3125
Fragments arranged by size:
3125 1071 898 85
BsmI GAATG_Cn'
Cuts at: 2690 2783 2690
Size: 93 5086
BsmBI CGTCTCn'nnnn_
Cuts at: 2204 2204
Size: 5179
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2261 2271 2342 2498 3240 3325
Size: 399 289 182 10 71 156 742 85
Cuts at: 3325 4396 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 289 182 156 85
71 10
BspEI T'CCGG_A
Cuts at: 4842 4842
Size: 5179
BspHI T'CATG_A
Cuts at: 3068 4798 3068
Size: 1730 3449
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2534 844
Size: 1690 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2084 2361 2464 3066 1394
Size: 429 261 277 103 602 3507
Fragments arranged by size:
3507 602 429 277 261 103
BsrDI GCAATG_nn'
Cuts at: 65 1248 2371 3674 3848 65
Size: 1183 1123 1303 174 1396
Fragments arranged by size:
1396 1303 1183 1123 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2109 2119 2410 3823 4010 1700
Size: 110 299 10 291 1413 187 2869
Fragments arranged by size:
2869 1413 299 291 187 110 10
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5179
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5179
BssSI C'ACGA_G
Cuts at: 3239 4251 3239
Size: 1012 4167
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5179
BstBI TT'CG_AA
Cuts at: 3983 3983
Size: 5179
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5179
BstXI CCAn_nnnn'nTGG
Cuts at: 2185 2185
Size: 5179
BstYI r'GATC_y
Cuts at: 1972 2509 2876 3261 3278 4730 4816 4828 5174
Size: 537 367 385 17 1452 86 12 346
Cuts at: 5174 1972
Size: 1977
Fragments arranged by size:
1977 1452 537 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 2151 2184 2304 513
Size: 552 939 66 81 33 120 3388
Fragments arranged by size:
3388 939 552 120 81 66 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4440
Fragments arranged by size:
4440 683 56
BtsI GCAGTG_nn'
Cuts at: 2695 3489 3509 2695
Size: 794 20 4365
Fragments arranged by size:
4365 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5179
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5144
DraI TTT'AAA
Cuts at: 2483 2483
Size: 5179
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4186 809
Size: 507 2870 1802
Fragments arranged by size:
2870 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 2167 2238
Size: 54 1425 50 275 18 300 37 71
Cuts at: 2238 2626 3515 5144 8
Size: 388 889 1629 43
Fragments arranged by size:
1629 1425 889 388 300 275 71 54
50 43 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 2130 2167 2626 1537
Size: 275 318 37 459 4090
Fragments arranged by size:
4090 459 318 275 37
EarI CTCTTCn'nnn_
Cuts at: 1353 2433 3109 1353
Size: 1080 676 3423
Fragments arranged by size:
3423 1080 676
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3766 4140 4286 387
Size: 1064 138 2177 374 146 1280
Fragments arranged by size:
2177 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2394 3242 3839 4626 4894 1239
Size: 393 482 280 848 597 787 268 1524
Fragments arranged by size:
1524 848 787 597 482 393 280 268
EcoO109I rG'GnC_Cy
Cuts at: 2075 2103 2137 2257 2258 2075
Size: 28 34 120 1 4996
Fragments arranged by size:
4996 120 34 28 1
EcoRI G'AATT_C
Cuts at: 2488 2866 2488
Size: 378 4801
FspI TGC'GCA
Cuts at: 2329 3685 2329
Size: 1356 3823
HaeII r_GCGC'y
Cuts at: 1678 2165 2192 4326 1678
Size: 487 27 2134 2531
Fragments arranged by size:
2531 2134 487 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3820 3852 3894 3926 1373
Size: 32 135 32 2248 32 42 32 2626
Fragments arranged by size:
2626 2248 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2523 2769 669
Size: 988 281 585 246 3079
Fragments arranged by size:
3079 988 585 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5179
HpaI GTT'AAC
Cuts at: 2769 2769
Size: 5179
KasI G'GCGC_C
Cuts at: 2161 2188 2161
Size: 27 5152
KpnI G_GTAC'C
Cuts at: 2504 2504
Size: 5179
MfeI C'AATT_G
Cuts at: 2778 2778
Size: 5179
MluI A'CGCG_T
Cuts at: 5055 5055
Size: 5179
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2320 4293 4477 1305
Size: 1015 1973 184 2007
Fragments arranged by size:
2007 1973 1015 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3357
Fragments arranged by size:
3357 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3137 3496 3655 518
Size: 648 1971 359 159 2042
Fragments arranged by size:
2042 1971 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2013 2072 2153 2306 2388 2595 3272 4420
Size: 407 59 81 153 82 207 677 1148
Cuts at: 4420 4665 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 407 245 207 153 82
81 59
NaeI GCC'GGC
Cuts at: 2111 2412 2111
Size: 301 4878
NarI GG'CG_CC
Cuts at: 2162 2189 2162
Size: 27 5152
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3688
Fragments arranged by size:
3688 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5179
NgoMIV G'CCGG_C
Cuts at: 2109 2410 2109
Size: 301 4878
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5179
NotI GC'GGCC_GC
Cuts at: 2626 2626
Size: 5179
NspI r_CATG'y
Cuts at: 1132 1229 2537 4082 1132
Size: 97 1308 1545 2229
Fragments arranged by size:
2229 1545 1308 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5179
PciI A'CATG_T
Cuts at: 4078 4078
Size: 5179
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5179
PmeI GTTT'AAAC
Cuts at: 2483 2483
Size: 5179
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3248 3280 4761 4793 3248
Size: 32 1481 32 3634
Fragments arranged by size:
3634 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2103 2137 2103
Size: 34 5145
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5179
PsiI TTA'TAA
Cuts at: 2749 2749
Size: 5179
PspOMI G'GGCC_C
Cuts at: 2075 2257 2075
Size: 182 4997
PstI C_TGCA'G
Cuts at: 830 2531 830
Size: 1701 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5179
SacII CC_GC'GG
Cuts at: 2073 2154 2307 2073
Size: 81 153 4945
Fragments arranged by size:
4945 153 81
SalI G'TCGA_C
Cuts at: 1936 2521 1936
Size: 585 4594
SanDI GG'GwC_CC
Cuts at: 2103 2103
Size: 5179
SbfI CC_TGCA'GG
Cuts at: 2531 2531
Size: 5179
ScaI AGT'ACT
Cuts at: 2471 3427 5139 2471
Size: 956 1712 2511
Fragments arranged by size:
2511 1712 956
SfcI C'TryA_G
Cuts at: 826 1045 2527 3662 4343 4534 826
Size: 219 1482 1135 681 191 1471
Fragments arranged by size:
1482 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2163 2190 2163
Size: 27 5152
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5179
SgrAI Cr'CCGG_yG
Cuts at: 1700 2109 1700
Size: 409 4770
SmaI CCC'GGG
Cuts at: 1872 2142 2355 2506 1872
Size: 270 213 151 4545
Fragments arranged by size:
4545 270 213 151
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3283 4184 4446 820
Size: 197 926 1340 901 262 1553
Fragments arranged by size:
1553 1340 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2970 493
Size: 2477 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5179
SphI G_CATG'C
Cuts at: 1132 2537 1132
Size: 1405 3774
SspI AAT'ATT
Cuts at: 5 52 3103 5
Size: 47 3051 2081
Fragments arranged by size:
3051 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5179
StyI C'CwwG_G
Cuts at: 513 1065 2004 2618 513
Size: 552 939 614 3074
Fragments arranged by size:
3074 939 614 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3206 3223 3376 3561 4006 325
Size: 1255 1626 17 153 185 445 1498
Fragments arranged by size:
1626 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2469 3425 5137
Size: 274 80 33 51 1443 492 956 1712
Cuts at: 5137 96
Size: 138
Fragments arranged by size:
1712 1443 956 492 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5139 5171 5139
Size: 32 5147
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5179
XbaI T'CTAG_A
Cuts at: 2515 2515
Size: 5179
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5179
XmaI C'CCGG_G
Cuts at: 1870 2140 2353 2504 1870
Size: 270 213 151 4545
Fragments arranged by size:
4545 270 213 151
XmnI GAAnn'nnTTC
Cuts at: 3308 3308
Size: 5179
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2987 276
Size: 53 83 186 936 1453 2468
Fragments arranged by size:
2468 1453 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SapI SexAI
SfiI SrfI SwaI XcmI